Comparison of Hyperbaric 0.5% Levobupivacaine and Hyperbaric 0.75% Ropivacaine for Intrathecal Use in Infraumbilical Surgeries: A Randomised Clinical Study by Tejash H Sharma, Jagrati Jain, Priya Kishnani, Richa Tailor, Sara Mary Thomas

“…It has less affinity and strength of depressant effects on myocardial and Central Nervous System (CNS) vital centers in pharmacodynamic studies, along with a superior pharmacokinetic profile. …”
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Large-scale manufacturing and characterization of CMV-CD19CAR T cells by Stephen J Forman, Xiuli Wang, Ryan Urak, Miriam Walter, Min Guan, Tianxu Han, Vibhuti Vyas, Sheng-Hsuan Chien, Brenna Gittins, Mary C Clark, Sally Mokhtari, Angelo Cardoso, Don J Diamond, John Zaia, Ryotaro Nakamura

“…Here we developed a clinical platform for generating CMV-CD19CAR T cells.Methods Peripheral blood mononuclear cells (PBMCs) collected from CMV-seropositive healthy donors were stimulated with a good manufacturing practices-grade PepTivator overlapping CMVpp65 peptide pool and enriched for CMV-responsive interferon γ (IFNγ)+T cells using IFNγ Catchmatrix, within the CliniMACS Prodigy Cytokine Capture System (Miltenyi Biotec). Resulting CMV-specific T cells were transduced with a lentiviral vector encoding a second generation CD19R:CD28:ζ/EGFRt CAR and expanded with interleukin 2 (IL-2) and IL-15 for 15 days before characterization.Results CMV-specific T cells were enriched from 0.8%±0.5 of input PBMC to 76.3%±11.6 in nine full-scale qualification runs (absolute yield of 4.2±3.3×106 IFNγ+T cells from an input of 1×109 PBMCs). …”
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Clinical Outcomes of Transcatheter Aortic Valve Replacement in Nonagenarians: A Systematic Review and Meta-Analysis by Yan Liu, Yu Du, Mingjie Fu, Yue Ma, Deguang Wang, Jinglin Zhang, Wei Liu, Yingxin Zhao, Yujie Zhou

“…Compared with younger patients who underwent TAVR, nonagenarians had a significantly higher mean Society of Thoracic Surgeons score (STS score) (MD, 2.80; 95%CI: 2.58, 3.30; P<0.00001) and logistic European System for Cardiac Operative Risk Evaluation (logistic EuroSCORE) (MD, 2.72; 95%CI: 1.01, 4.43; P=0.002). …”
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The transcription factor combination MEF2 and KLF7 promotes axonal sprouting in the injured spinal cord with functional improvement and regeneration-associated gene expression by Callan L. Attwell, Inés Maldonado-Lasunción, Ruben Eggers, Bastiaan A. Bijleveld, Ward M. Ellenbroek, Natascha Siersema, Lotte Razenberg, Dédé Lamme, Nitish D. Fagoe, Ronald E. van Kesteren, August B. Smit, Joost Verhaagen, Matthew R. J. Mason

“…Abstract Background Axon regeneration after injury to the central nervous system (CNS) is limited by an inhibitory environment but also because injured neurons fail to initiate expression of regeneration associated genes (RAGs). …”
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FTO rs17817449 Variant Increases the Risk of Severe Obesity in a Brazilian Cohort: A Case-Control Study by Salum KCR, Assis ISS, Kopke ÚA, Palhinha L, Abreu GM, Gouvêa LW, Teixeira MR, Mattos FCC, Nogueira Neto JF, Felício RFM, Rosado EL, Zembrzuski VM, Campos Junior M, Maya-Monteiro CM, Cabello PH, Carneiro JRI, Bozza PT, Kohlrausch FB, da Fonseca ACP

“…Kaio Cezar Rodrigues Salum,1,2 Izadora Sthephanie da Silva Assis,1,2 Úrsula de Almeida Kopke,3 Lohanna Palhinha,4 Gabriella de Medeiros Abreu,2,5 Laura Wendling Gouvêa,1,2 Myrela Ribeiro Teixeira,2,6,7 Fernanda Cristina C Mattos,5 José Firmino Nogueira Neto,8 Rafaela de Freitas Martins Felício,9,10 Eliane Lopes Rosado,5 Verônica Marques Zembrzuski,2 Mario Campos Junior,2 Clarissa Menezes Maya-Monteiro,4 Pedro Hernán Cabello,2 João Regis Ivar Carneiro,1 Patrícia Torres Bozza,4 Fabiana Barzotto Kohlrausch,6 Ana Carolina Proença da Fonseca2,4,11 1Medical Clinic Department, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; 2Human Genetics Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil; 3Chester Medical School, University of Chester, Chester, UK; 4Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Rio de Janeiro, Brazil; 5Institute of Nutrition Josué de Castro, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; 6Human Genetics Laboratory, Institute of Biology, Federal Fluminense University Niterói, Rio de Janeiro, Brazil; 7Postgraduate in Sciences and Biotechnology, Fluminense Federal University Niterói, Rio de Janeiro, Brazil; 8Department of Pathology and Laboratory, Rio de Janeiro State University, Rio de Janeiro, Brazil; 9Birth Defect Epidemiology laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil; 10Health Care Network for Congenital Anomalies of the Central Nervous System, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil; 11Genetics Laboratory, Grande Rio University, Rio de Janeiro, BrazilCorrespondence: Ana Carolina Proença da Fonseca, Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), 4365 Brasil Avenue, 108 Building – Office 44, Rio de Janeiro, 21040-360, Brazil, Tel +55(21)38658192, Email ana.proenca@ioc.fiocruz.br Kaio Cezar Rodrigues Salum, Human Genetics Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz), 4365 Brasil Avenue, Leônidas Deane Building – Office 611/615, Rio de Janeiro, 21040-360, Brazil, Tel +55 21 38658213, Fax +55 21 38658239, Email kaio.salum@hotmail.comPurpose: Obesity is a complex disease caused by a combination of genetic, environmental, and epigenetic factors, and is associated with an increased risk of chronic diseases. …”
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Effects of fine particulate matter and its chemical constituents on influenza-like illness in Guangzhou, China by Keyi Wu, Weidong Fan, Jing Wei, Jianyun Lu, Xiaowei Ma, Zelin Yuan, Zhiwei Huang, Qi Zhong, Yining Huang, Fei Zou, Xianbo Wu

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A Pool of Bacterium-like Particles Displaying African Swine Fever Virus Antigens Induces Both Humoral and Cellular Immune Responses in Pigs by Jingshan Huang, Hongxia Wu, Tianqi Gao, Huanjie Zhai, Assad Moon, Xin Song, Shuwen Li, Zhanhao Lu, Jing Lan, Dailang Zhong, Xinyu Zhang, Hua-Ji Qiu, Yongfeng Li, Yuan Sun

“…Methods: Here, we constructed five bacterium-like particles (BLPs) that each display one of the five ASFV antigens (F317L, H171R, D117L, B602L, and p54) based on the Gram-positive enhancer matrix-protein anchor (GEM-PA) system. GEM is a bacterial particle that contains only peptidoglycan, while PA is composed of three lysin motifs (Lysm) derived from the C-terminus of the AcmA protein, capable of non-covalently binding to GEM. …”
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Clinical Significance of Perioperative Minimal Residual Disease Detected by Circulating Tumor DNA in Patients With Lung Cancer With a Long Follow-up Data: An Exploratory Study by Shuta Ohara, MD, PhD, Kenichi Suda, MD, PhD, Sumedha Sudhaman, PhD, Akira Hamada, MD, PhD, Masato Chiba, MD, PhD, Masaki Shimoji, MD, PhD, Toshiki Takemoto, MD, PhD, Ekaterina Kalashnikova, PhD, Samantha K. Cheung, PhD, Michael Krainock, MD, PhD, Jordan Feeney, BS, Himanshu Sethi, MPH, Minetta C. Liu, MD, Junichi Soh, MD, PhD, Yasuhiro Tsutani, MD, PhD, Tetsuya Mitsudomi, MD, PhD

“…Three patients who had central nervous system–only metastases did not have detectable ctDNA. …”
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Efek Pengurangan Durasi Tidur terhadap Jumlah dan Hitung Jenis Leukosit pada Tikus Wistar by Wahyu Tri Novriansyah, Ellyza Nasrul, Rosfita Rasyid

“…Sleep deprivation affects the immune system as well as the disruption of circadian rhythms. …”
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Cancer care in German centers of excellence during the first 2 years of the COVID-19 pandemic by Volker Arndt, Daniela Doege, Stefan Fröhling, Peter Albers, Hana Algül, Ralf Bargou, Carsten Bokemeyer, Martin Bornhäuser, Christian H. Brandts, Peter Brossart, Sara Yvonne Brucker, Tim H. Brümmendorf, Hartmut Döhner, Norbert Gattermann, Michael Hallek, Volker Heinemann, Ulrich Keilholz, Thomas Kindler, Cornelia von Levetzow, Florian Lordick, Ulf Peter Neumann, Christoph Peters, Dirk Schadendorf, Stephan Stilgenbauer, Thomas Zander, Daniel Zips, Delia Braun, Thomas Seufferlein, Gerd Nettekoven, Michael Baumann

“…Results The COVID-19 pandemic substantially affected the oncological care system in Germany during the first 2 years. Persistent limitations of care in CCCs primarily affected follow-up (− 21%) and psycho-oncologic care (− 12%), but also tumor surgery (− 9%). …”
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Adhesive micro-liquid for efficient removal of bacterial biofilm infection by Ying Wang, Zhibang Li, Lingli Ji, Jiao Sun, Fei Gao, Ruiqing Yu, Kai Li, Wenjun Wang, Weiwei Zhao, Qi-Zhi Zhong, Shaohua Ge, Jianhua Li

“…The proposed adhesive micro-liquid system offers a promising strategy for noninvasive and efficient removal of bacterial biofilm infection.…”
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Integrated microfluidics-based construction of anti-BTN2A2 gel droplet cell preparations for non-invasive tumor-infiltrating lymphocyte therapy by Yishen Tian, Jingxuan Li, Na Yang, Yang Zhao, Jiancao Zuo, Hang Xiong, Yiwen Pan, Li Xiao, Min Su, Feng Han, Zhixu He, Rong Hu

“…In the complex immunosuppressive microenvironment, the synergistic action of these components markedly enhanced the clearance efficacy of the immune system. Experimental results demonstrated that this cellular preparation exhibited promising therapeutic effects in both melanoma and pancreatic cancer models. …”
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Zolpidem: Efficacy and Side Effects for Insomnia by Amber N. Edinoff, Natalie Wu, Yahya T. Ghaffar, Rosemary Prejean, Rachel Gremillion, Mark Cogburn, Azem A. Chami, Adam M. Kaye, Alan D. Kaye

“…A case series of 119 inpatients aged 50 or older demonstrated that a majority (80.8%) of ADRs were central nervous system (CNS)-related such as confusion, dizziness, and daytime sleepiness. …”
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Macrophages facilitate tumor cell PD‐L1 expression via an IL‐1β‐centered loop to attenuate immune checkpoint blockade by Cheng Xu, Yu Xia, Bai‐Wei Zhang, Emmanuel Kwateng Drokow, Hua‐Yi Li, Sen Xu, Zhen Wang, Si‐Yuan Wang, Ping Jin, Tian Fang, Xiao‐Ming Xiong, Pu Huang, Ning Jin, Jia‐Hong Tan, Qing Zhong, Yu‐Xin Chen, Qi Zhang, Yong Fang, Fei Ye, Qing‐Lei Gao

“…Here, we revealed that interleukin (IL)‐1β was among the most abundant cytokines within the in vitro tumor‐macrophage coculture system, and enhanced IL‐1β expression was associated with impaired cytotoxicity of CD8+ T cells in human ovarian cancer, indicating the possibility that IL‐1β mediated immunosuppression during tumor‐TAMs crosstalk. …”
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Kontrol Level Kecepatan Kipas Melalui Deteksi Gestur Jari Tangan Menggunakan MediaPipe dan Faster-RCNN by Muhammad Aldi Fakhruddin, Heri Pratikno, Musayyanah, Weny Indah Kusumawati

“…In this study, an interaction control system was applied between the user and the computer to adjust the fan speed level automatically and in real time based on computer vision technology for deep learning through the detection of the shape of the right hand finger gesture and the left hand finger gesture using a webcam. …”
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Synaptotagmin-1 attenuates myocardial programmed necrosis and ischemia/reperfusion injury through the mitochondrial pathway by Teng Sun, Jialei Li, Shuang Wang, Yu Han, Xiangyu Tao, Min Yuan, Zhijie Jing, Ting Liu, Yuehong Qi, Siqi Liu, Yanlin Feng, Jiasong Chang, Lan Zhou, Lijuan Gao, Jianyun Shi, Ruihong Ning, Jimin Cao

“…Synaptotagmin-1 (Syt1), a Ca2+ sensor, is originally identified in nervous system and mediates synchronous neurotransmitter release. …”
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Saturated hydraulic conductivity and steady-state infiltration rate database for Brazilian soils by Marta Vasconcelos Ottoni, Wenceslau Geraldes Teixeira, Aline Mari Huf dos Reis, Letícia Guimarães Pimentel, Luciana Rodrigues Souza, Jackson Adriano Albuquerque, Valdinar Ferreira Melo, Karina Maria Vieira Cavalieri-Polizeli, José Miguel Reichert, João Herbert Moreira Viana, Ademir Fontana, Lucas de Castro Medrado, Glenio Guimarães Santos, Luís Gustavo Henriques do Amaral, Lúcia Helena Cunha dos Anjos, José Coelho de Araújo Filho, Silvio Barge Bhering, Gabrielle Fernandes de Brito, Pedro Gomes de Campos do Valle, Pablo Nieto Campos, Adriana Monteiro da Costa, Jean Dalmo de Oliveira Marques, Alba Leonor da Silva Martins, Michele Bruna de Souza do Nascimento, Norberto Cornejo Noronha, Ricardo Duarte de Oliveira, Jeane Cruz Portela, Milson Evaldo Serafim, Marlen Barros e Silva, Sueli Rodrigues, Wilk Sampaio de Almeida, Margareth Lopes de Moraes, Nilton Curi

“…The two groupings that presented the lowest variability in terms of Ksat standard deviation values were the combination of textural classes with soil classes at the second category level of SiBCS (Brazilian Soil Classification System), and porosity classes with soil classes at the second category level of SiBCS. …”
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Effects of taurine on growth performance, body composition and physiological responses of western white shrimp, Litopenaeus vannamei broodstock by Vahid Morshedi, Mahmoud Nafisi Bahabadi, Mansour Torfi Mozanzadeh, Amin Oujifard, Naser Agh, Khalegh Maneii, Ahmad Qasemi, Nehzat Bakhshi, Hadi Ebrahimi, Rezvan Tamadoni, Shirin Hamedi, Reza Bagherpour, Fatemeh Solimani

“…Following two weeks of acclimatization to the husbandry system, the shrimp broodstock were placed in two 4000-L circular fiberglass tanks. …”
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The Impact of the COVID-19 Pandemic on Health and Health Care Experience in Those With Polycystic Ovary Syndrome by Sydora BC, Wilke MS, Ghosh M, Vine DF

“…COVID-19 caused unprecedented challenges to the health care system but also presented opportunities for alternative health care delivery models. …”
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Targeted allele-specific FGFR2 knockdown via human recombinant ferritin nanoparticles for personalized treatment of Crouzon syndrome by Federica Tiberio, Martina Salvati, Luca Polito, Giada Tisci, Alessia Vita, Ornella Parolini, Luca Massimi, Lorena Di Pietro, Pierpaolo Ceci, Gianpiero Tamburrini, Alessandro Arcovito, Elisabetta Falvo, Wanda Lattanzi

“…We developed a highly biocompatible delivery system for siRNAs, based on human recombinant ferritin nanoparticles (NPs), combining cell targeting with improved nucleic acid encapsulation and endosomal escape properties. …”
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