SPA14 liposomes combining saponin with fully synthetic TLR4 agonist provide adjuvanticity to hCMV vaccine candidate by Ernesto Luna, Sophie Ruiz, Marie Garinot, Cyril Chavagnac, Pankaj Agrawal, John Escobar, Laurent Revet, Marie-Jeanne Asensio, Fabienne Piras, Francis G. Fang, Donald R. Drake, Bachra Rokbi, Daniel Larocque, Jean Haensler

“…The resulting adjuvant system, termed SPA14, appeared as a long-term stable and homogeneous suspension of mostly unilamellar and a few multilamellar vesicles, with an average hydrodynamic diameter of 93 nm, when formulated in citrate buffer at pH 6.0-6.5. …”
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Integrate mathematical modeling for heat dynamics in two-phase casson fluid flow through renal tubes with variable wall properties by P. Deepalakshmi, Adil Darvesh, Hakim AL Garalleh, Manuel Sánchez-Chero, G. Shankar, E.P. Siva

“…The particle phase models the behavior of stiff calculi suspensions due to lack of filtration process. The simulations illustrate that electromagnetic radiation can effectively manipulate the pumping properties in urology. …”
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Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice by Ali Yousefian-Jazi, Suhyun Kim, Jiyeon Chu, Seung-Hye Choi, Phuong Thi Thanh Nguyen, Uiyeol Park, Min-gyeong Kim, Hongik Hwang, Kyungeun Lee, Yeyun Kim, Seung Jae Hyeon, Hyewhon Rhim, Hannah L. Ryu, Grewo Lim, Thor D. Stein, Kayeong Lim, Hoon Ryu, Junghee Lee

“…Behavioral changes of mice were examined through longitudinal study by tail suspension, inverted grid test and automated gait analysis. …”
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Deciphering molecular and cellular ex vivo responses to bispecific antibodies PD1-TIM3 and PD1-LAG3 in human tumors by Alfred Zippelius, Petra Herzig, Pratiksha Gulati, Christian Klein, Marta Trüb, Kirsten D Mertz, Robert Rosenberg, Viola Heinzelmann-Schwarz, Mark Wiese, Didier Lardinois, Pablo Umana, Marina Natoli, Klas Hatje, Fabian Junker, Zhiwen Jiang, Iakov I Davydov, Markus Germann, Daniel Marbach, Adrian Zwick, Patrick Weber, Stefan Seeber, Lothar Tietze, Laura Codarri-Deak, Henry Kao

“…Similarly, little is known on the differential effects of dual, compared with single, checkpoint inhibition.Methods We here performed in-depth characterization, including multicolor flow cytometry, single cell RNA sequencing and multiplex supernatant analysis, using tumor single cell suspensions from patients with cancer treated ex vivo with novel bispecific antibodies targeting programmed cell death protein 1 (PD-1) and TIM-3 (PD1-TIM3), PD-1 and LAG-3 (PD1-LAG3), or with anti-PD-1.Results We identified patient samples which were responsive to PD1-TIM3, PD1-LAG3 or anti-PD-1 using an in vitro approach, validated by the analysis of 659 soluble proteins and enrichment for an anti-PD-1 responder signature. …”
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Development and evaluation of optimized PCR and indirect ELISA for the detection of Morganella morganii in dairy cows by Meihua Zhang, Jiayi Li, Jianfeng Xue, Huiling Xu, Muzi Li, Yibo Xia, Changxi Qi, Pu Zhang, Yongxia Liu, Jianzhu Liu

“…Currently, detection methods for M. morganii are underdeveloped, prompting us to develop both pathogenic and serological detection methods, including an optimized PCR technique and an indirect enzyme-linked immunosorbent assay (I-ELISA).MethodsThe optimized PCR method utilized bacterial suspensions directly as templates, bypassing the need for DNA extraction and thereby allowing the direct detection of M. morganii in fecal samples. …”
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Implementation of Accurate and Fast DNA Cytometry by Confocal Microscopy in 3D by Lennert S. Ploeger, André Huisman, Jurryt van der Gugten, Dionne M. van der Giezen, Jeroen A. M. Beliën, Abdelhadi Y. Abbaker, Hub F. J. Dullens, William Grizzle, Neal M. Poulin, Gerrit A. Meijer, Paul J. van Diest

“…This provides a suitable method for 3D DNA ploidy assessment of selected rare cells based on morphologic characteristics and of clinical samples that are too small to prepare adequate cell suspensions.…”
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Visual analysis of research hotspots and trends in traditional Chinese medicine for depression in the 21st century: A bibliometric study based on citespace and VOSviewer by Chengcheng Song, Kelong Chen, Yongxi Jin, Ling Chen, Zuxiu Huang

“…Before 2010, these keywords included the forced swimming test, Tail Suspension Test, Chronic Cold Stress, Neural Regeneration, and Banxia Houpu Decoction. …”
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Le lymphosarcome fausse les résultats du test de la tuberculose effectué sur le sang by Cheick Coulibaly, Julia Seidl, Bettina Bernhard, Roland Plesker

“…Les institutions dont les colonies sont testées positives risquent la suspension ou la perte de l’autorisation de détenir des primates. …”
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Therapeutic Effect of Core Stability Training Combined with Extracorporeal Shock Wave on Lumbar Disc Herniation by Shuo LI, Lishuang LIU, Jinna ZHOU, Qiaoyun XIANG, Tingting LUAN, Wenwen WANG

“…SPS-F11 multi-point multi-axis suspension rehabilitation training system was applied, under the guidance of the same rehabilitation doctor. …”
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Betulinic Acid Inhibits the Metastasis of Colon Cancer Cells through Regulating TGF-β/Smad Signaling Pathway by Xiaoying LIN, Aling SHEN, Meizhu WU, Ying CHENG, Xiaoping CHEN, Huixin LIU, Liya LIU, Zhiqing SHEN, Xiangyan WU, Nanhui XU, Jianfeng CHU, Youqin CHEN, Jun PENG

“…Then the cells were collected by digestion and centrifugation after treating with different concentrations (0,10,20, and 40 μmol/L) of BA for 48 h.1.0×10⁵cells/mL cell suspension was used for the Transwell experiment of cell migration and invasion, respectively. …”
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April 15th war and hemodialysis patients in Sudan: a cross-sectional study by Malaz Hassan Dafaalla Idrees, Moez Mohammed Ibrahim Bashir, Balqees Abdeen Ahmed Mohamed, Amal Essam Ali Ahmed, Hamid Mohieldin Mohamed Abdalla, Kamil Mirghani Ali Shaaban

“…Hemodialysis patients are among the most affected by this war’s aftermath, which ranges from obvious dangers like violence-related injuries and deaths to more indirect effects like the suspension of access to preventive and curative medical care. …”
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Clinical Observation on Chronic Low Back Pain Treated by Sling and Tuina Exercise Therapy by Ning WANG, Yi DING, Qiangqiang XU, Jiapeng ZHANG, LI Li

“…The sling exercise therapy group received active sports training under limb suspension with sling in the lateral position, the prone position and the supine position. …”
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Optimization and Evaluation of Conditions for Orthotopic Nude Mouse Models of Human Liver Tumor Cells by MENG Yu, LIANG Dongli, ZHENG Linlin, ZHOU Yuanyuan, WANG Zhaoxia

“…Different concentrations (8×106, 2.4×107, 7.2×107 cells/mL) and resuspension media (PBS, Matrigel) of human liver tumor cell suspensions HepG2-LUC and Hep3B-LUC were orthotopically inoculated into the liver lobes of 5-week-old female BALB/c nude mice (12 groups, 7 mice each) to construct human liver tumor nude mouse orthotopic cancer models. …”
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hBMSC-EVs alleviate weightlessness-induced skeletal muscle atrophy by suppressing oxidative stress and inflammation by Mengyuan Chang, Ruiqi Liu, Bingqian Chen, Jin Xu, Wei Wang, Yanan Ji, Zihui Gao, Boya Liu, Xinlei Yao, Hualin Sun, Feng Xu, Yuntian Shen

“…C2C12 myotube nutrition-deprivation and mice tail suspension models were established. Subsequently, the diameter of C2C12 myotubes, Soleus mass, cross-sectional area (CSA) of muscle fibers, and grip strength in mice were assessed to investigate the impact of hBMSC-EVs on muscle atrophy. …”
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Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies by Paula L. Marcet, Brandon Short, Ashley Deas, Hong Sun, Chelsea Harrington, Shahzad Shaukat, Muhammad Masroor Alam, Marycelin Baba, Adedayo Faneye, Prossy Namuwulya, Lea Necitas Apostol, Tamer Elshaarawy, John Kofi Odoom, Peter Borus, Shelina Moonsamy, Yogolelo Riziki, Marie Claire Endegue Zanga, Mesfin Tefera, Anfumbom K. W. Kfutwah, Salmaan Sharif, Varja Grabovac, Cara C. Burns, Nancy Gerloff

“…Strategically selected laboratories were tasked to simultaneously process stool suspensions with the current gold-standard VI method and the new DD-ITD strategy. …”
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<i>Cichorium intybus</i> L. Oligo-Polysaccharides (CIO) Exerts Antianxiety and Antidepressant Effects on Mice Experiencing Behavioral Despair and Chronic Unpredicted Mild Stress by Yanqin Luo, Xueyi Bei, Yiwen Zhang, Xinran Sun, Yongzhi Zhao, Fang Chen, Ruile Pan, Qi Chang, Qinghu He, Xinmin Liu, Ning Jiang

“…They were randomized into seven groups (Control, Citalopram, CIO 12.5 mg/kg, CIO 25 mg/kg, CIO 100 mg/kg, and CIO 200 mg/kg) based on body weight; they were administered with the corresponding medication daily for 7 days; and behavioral tests were conducted on them (forced swimming test (FST) and tail suspension test (TST)) after 7 days. Seventy male C57BL/6N mice were adopted in the next part of the experiment and randomly divided into seven groups (Control, CUMS, Fluoxetine, MOO, CIO 25 mg/kg, and CIO 100 mg/kg) based on the sucrose preference index. …”
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Inhalation d'aérosols liquides ou solides chez les Primates Non Humains by Jérôme Montharu, Chrystophe Aubert, Georges Roseau, Laurent Vecellio, Antoine Guillon, Michèle de Monte

“…L'administration d'aérosols médicamenteux liquides et solides - « particules suffisamment fines pour rester en suspension dans l'air », connaît depuis quelques années une forte expansion. …”
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Development of Solid Self-Nanoemulsifying Drug Delivery System of Rhein to Improve Biopharmaceutical Performance: Physiochemical Characterization, and Pharmacokinetic Evaluation by More SM, Rashid MA, Kharwade RS, Taha M, Alhamhoom Y, Elhassan GO, Gangane P, Asar TO, Pise A, Kaleem M, Mujtaba MA

“…Moreover, the in vivo pharmacokinetic study showed a significantly higher (p ≤ 0.05) value of Cmax and AUC0-t of RS-SNEDDS (8 ± 0.930 μg/mL and 37.79 ± 2.01 μg/mL&ast;hr) compared to free rhein suspension (1.96 ± 0.712 μg/mL and 7.32 ± 0.946 μg/mL&ast;hr) which indicated the enhancement of bioavailability of RS-SNEDDS. …”
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Unveiling the Mechanisms of a Remission in Major Depressive Disorder (MDD)-like Syndrome: The Role of Hippocampal Palmitoyltransferase Expression and Stress Susceptibility by Careen A. Schroeter, Anna Gorlova, Michael Sicker, Aleksei Umriukhin, Alisa Burova, Boris Shulgin, Sergey Morozov, Joao P. Costa-Nunes, Tatyana Strekalova

“…The stress protocol consisted of rat exposure, social defeat, restraint stress, and tail suspension. Mice from the stress group were not treated, received imipramine via drinking water (7 mg/kg/day), or received intraperitoneal injections of dicholine succinate (DS; 25 mg/kg/day) starting 7 days prior to stress and continuing during a 14-day stress procedure. …”
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Geopolitical (non) opportunities vs. "late" response of the foreign political service of North Macedonia in the process of European integration by Jelisavac-Trošić Sanja, Arnaudov Mitko

“…The postponement of North Macedonia's accession to NATO in 2008, due to Greece's express refusal, with a focus on connecting the Macedonian nation with the heritage of the ancient Macedonians, with the simultaneous suspension of democratic capacities and principles that represent significant determinants in the process of joining the European Union, leads to missing out and moving away from the path accelerated European integration of North Macedonia. …”
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