Exploring the Genetic Landscape of Mycobacterium tuberculosis: Unlocking the Differences in Between Latent and Active Tuberculosis by Ali Akbar Velayati, Satoshi Mitaria, Parissa Farnia, Poopak Farnia, Jaladein Ghanavi, Mohsan Maleknia, Yoshiro Murase, Sven Hoffner

“…., H37Rv and Mycobacterium bovis bacillus Calmette–Guérin. Each strain was subcultured in a controlled laboratory environment to analyze two distinct phases: one maintained in vitro under a double-stress dormancy model using the Wayne model (characterized by the absence of oxygen and nutrients) for 12 months, and the other examined during its exponential growth phase. …”
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Efficient Micropropagation of Bistorta amplexicaulis (D.Don) Greene: An Important Medicinal Plant of Western Himalaya by Mudasir Fayaz, Seema Singh, Irshad Ahmad Bhat, Musadiq Hussain Bhat, Firdous Ahmad Khanday, Alamgir Ahmad Dar

“…Shoot multiplication was widely affected by the kind and concentration of PGRs, and the subculturing of in vitro regenerated shootlets on fresh medium. …”
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Testing and identification of bovine viral diarrhea virus isolates recovered in Russia between 2019 and 2022 by R. I. Bubyakin, S. V. Kononova, I. N. Shumilova, O. P. Byadovskaya, A. O. Krotova, A. V. Kononov

“…The recovery of these isolates in the lamb testicle cell subculture revealed that Bashkiria/2019, Kirov/2020 and Samara/2020 isolates belong to non-cytopathic bovine viral diarrhea virus biotypes, Chelyabinsk/2021 isolate demonstrated the characteristic cytopathic effect in the monolayer and was classified as a cytopathic variant of thevirus, the adaptation of Belgorod/2021 and Udmurtiya/2020 isolates to this cell system was not possible. …”
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Studies on banana for propagation, conservation and genome analysis by Susmita Shukla, Ritambhara Bhutani, Nibha Gupta, Shiv Kant Shukla, Taramla Raman, Mohamed A. El-Sheikh, Hosam O. Elansary, Ihab Mohamed Moussa

“…The highest number of shoots were obtained in 6th subculturing cycle thereafter each in vitro shoot of Musa acuminata var. hazari, Musa paradisica var. kashkol, Musa paradisica var. sanikol, and Musa acuminata var. hondakol were inoculated in 1/4th MS medium supplemented with 2 mgL-1 indole-3-butyric acid (IBA) and 1 mgL-1 BAP resulted in successful rooting of 43%, 45%, 60%, and 33% respectively. …”
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In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications by Guoguang Niu, John P. McQuilling, Yu Zhou, Emmanuel C. Opara, Giuseppe Orlando, Shay Soker

“…The cells proliferated well in tissue culture plates and were subcultured for no more than 5 passages. Only 10% of insulin expression, as measured by PCR, was preserved in each passage. …”
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Microbiological Profile of Neonatal Sepsis at a Maternity Hospital in Omdurman, Sudan by Manal Abdelaziz, Yassir Hamadalnil, Omaima Hashim, Tahane Bashir, E. S. Mahjoub

“…Specimens were cultured in Brain Heart Infusion broth followed by subculture of isolates on blood agar, MacConkey agar, and Chocolate agar and incubated aerobically at 37ºC for 24 h. …”
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SOCIAL ATTITUDES TO DRUGS ABUSE AMONG YOUTH AND DRUG ABUSE PREVENTION by Y. N. Nakhimova, G. F. Romashkina

“…Methodological basis of work involves classical theories of social installation; anomies; cultural conflict; subcultures; stigmatizations; social control; social space. …”
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Transmission of potato spindle tuber viroid between <i>Phytophthora infestans</i> and host plants by O. S. Afanasenko, A. V. Khiutti, N. V. Mironenko, N. M. Lashina

“…PSTVd was not detected after the eighth passage on rye agar in P. infestans subculture. These results are initial evidence of potato viroid PSTVd being bidirectionally transferred between P. infestans and host plants.…”
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Comparison of Rapidly Proliferating, Multipotent Aortic Valve-Derived Stromal Cells and Valve Interstitial Cells in the Human Aortic Valve by Yuming Huang, Kang Xu, Tingwen Zhou, Peng Zhu, Nianguo Dong, Jiawei Shi

“…In this study, culture conditions for mesenchymal stromal cells were used to selectively isolate valve-derived stromal cells (VDSCs). After subculturing, the morphology, proliferation, multidifferentiation, immunophenotype, and gene expression profiling in isolated VDSCs were compared with those in conventional cultured VICs. …”
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Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase by Amrit Koirala, Nabilah Ali Alshibli, Bikram K. Das, Volker S. Brözel

“…Putative nitrogen fixers were confirmed by subculturing in liquid nitrogen-free media and PCR amplification of the <i>nifH</i> genes. …”
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Assessing the Efficacy of Broad-Spectrum Antibiotics in Controlling Bacterial Contamination in the In Vitro Micropropagation of Ginger (Zingiber officinale Rosc) by Selam Tewelde, Subban Patharajan, Zenebe Teka, Desta Berhe Sbhatu

“…But, they turned green and grew normal after subcultured into fresh, antibiotics-free culture media. …”
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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…These strains demonstrated a high bioluminescence level (108–109 Photons/s/cm2/sr) and were tested for growth and plasmid stability by daily subculturing in Luria–Bertani medium with and without antibiotics. …”
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Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia by Mohabaw Jemal, Teshiwal Deress, Teshome Belachew, Yesuf Adem

“…If samples are positive for bacterial agents, they were subcultured to solid media such as blood agar plate, chocolate agar plate, and MacConkey agar plates. …”
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Induction of Somatic Embryogenesis and Organogenesis in Zimbabwean Sweet Potato (cv Brondal) by Rose T. Masekesa, Edmore Gasura, Gaudencia T. Kujeke, Elizabeth Ngadze, Farisai Chidzwondo

“…In contrast, plantlets produced under organogenesis were strong, grew vigorously, and could be subcultured several times. This disparity may be accounted for by the fact that the cv Brondal embryos that developed under SE were not exposed to an embryo maturation staged before plantlet germination was initiated. …”
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Establishment and characterization of a new mouse gastric carcinoma cell line, MCC by Yushen Wang, Xianju Li, Yi Wang, Jun Qin

“…Fresh gastric cancer tissue samples were cultured in 1640 medium supplemented with 10% fetal bovine serum for primary culture and subculture. Cellular morphology was assessed via light microscopy, and a cell growth curve was established. …”
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The rapid detection of a neonatal unit outbreak of a wild-type Klebsiella variicola using decentralized Oxford Nanopore sequencing by Rhys T. White, Michelle Balm, Megan Burton, Samantha Hutton, Jamaal Jeram, Matthew Kelly, Donia Macartney-Coxson, Tanya Sinha, Henrietta Sushames, David J. Winter, Maxim G. Bloomfield

“…Results Within 48 h of notification isolates had been subcultured and sequenced. This showed that three of four isolates were in fact K. variicola, and two of these were sequence type (ST)6385. …”
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Vertical Transmission of Group B Streptococcus, Prevalence, Associated Factors, and Antimicrobial Susceptibility Profile among Newborns Delivered at Health Facilities in Jigjiga Ci... by Addisu Tesfaye, Mahlet Fekede, Fasil Getu, Surafel Mekuria, Tigist Abebe, Daniel Tollosa, Shamil Barsenga, Bawlah Tahir, Abdurahman Kedir Roble, Sara Tesfaye, Muluken Walle

“…Samples were inoculated on Todd Hewitt broth media supplemented with gentamycin and nalidixic acid and then subcultured on blood agar. Colony characteristics, Gram stain, and catalase test were used for identification. …”
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Designing an educational model based on identity development with an Iranian-Islamic approach for primary school students. by Ali Oladhamzehzadeh, masoumeh oladiyan, Mahmoud Safari

“…The findings of the research showed several concepts and categories, including individual factors (developmental tasks and self-confidence), family factors (family growth-oriented functioning, efficient family characteristics and dysfunctional relationships in the family), cultural factors (opportunities and threats of the educational system, and cultural facilitator factors), social factors (respect for subcultures and identity-building patterns), and political-economic factors (weakness of political trust and macrosystem support). …”
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