Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Influence of catheter ablation for atrial fibrillation on atrial and ventricular functional mitral regurgitation by Masaharu Masuda, Kimiko Sekiya, Mitsutoshi Asai, Osamu Iida, Shin Okamoto, Takayuki Ishihara, Kiyonori Nanto, Takashi Kanda, Takuya Tsujimura, Yasuhiro Matsuda, Yosuke Hata, Hiroyuki Uematsu, Taku Toyoshima, Naoko Higashino, Toshiaki Mano

“…Abstract Aims The purpose of this study was to compare the impact of catheter ablation on cardiac structural reverse remodelling and atrial (AFMR) and ventricular (VFMR) functional mitral regurgitation (MR), and the long‐term prognosis of patients with AFMR and VFMR. …”
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Investigating slope stability of multiple stopes prone to instability in the Ziluoyi iron ore mining site by Zhongao Yang, Ziling Song, Xiaohua Ding, Mvondo Nga Michele Victoire, Ali Moussa Abdoul Wahab, Barry Oumar, Fanghui Yang, Abdullahi Yusuf Ibrahim, Zhen Gao, Zhaoxi Long

“…An effective algorithm based on the Morgenstern–Price method is employed and its effectiveness is also proved by comparing its factor of safety results with those of Geo-Slope for various slope structures subjected to different loads. Subsequently, the stability analyses of various slopes are methodically examined by predicting the factor of safety values under multiple types of loading (i.e., self-weight + groundwater, slope self-weight + groundwater + blasting vibration force, and self-weight + groundwater + seismic force). …”
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Sex-dependent efficacy of sphingosine-1-phosphate receptor agonist FTY720 in mitigating Huntington’s disease by Jingyun Wu, Irene Santos-Garcia, Ivan Eiriz, Thomas Brüning, Aleš Kvasnička, David Friedecký, Tuula A. Nyman, Jens Pahnke

“…FTY intervention also rescued testicular atrophy, restored testis tissue structure in male mice suggesting a broader impact on peripheral tissues affected by huntingtin pathology. …”
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Unveiling the ultimate advantage: a meta-analysis of 3D visualization and problem-based learning in orthopedic education by Ting Li, Yidan Hu, Ruohong Song, Hangyu Liu, Xin Gao, Fei Wang, Chengwei Xiao, Xilin Liu

“…Furthermore, students exposed to 3D + PBL teaching exhibited a significantly better understanding of orthopedic anatomical structures compared to those receiving LBL (SMD = 1.18, 95% CI: 0.71–1.65, P < 0.00001). …”
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Inhibition Mechanism of Pleurotus citrinipileatus Sing. Ergothioneine on Pancreatic Lipase Activity by Zhangchen XIA, Xiaohui MENG, Ru FANG, Yanqing MAO, Junwen CHENG, Yanbin WANG, Juan XU, Liang HE

“…Fluorescence spectra data revealed PCEGT could efficiently static-quench the intrinsic fluorescence of PL by changing its space structure. Based on the Lineweaver-Burk equation, the binding constants (Ka) under three different temperatures were obtained. …”
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Green Synthesis of Nanostructure CeO2 Using Tea Extract: Characterization and Adsorption of Dye from Aqueous Phase by Chengshun Liu, Xiyao Liu, Yilin Wu, Zhuotong Chen, Zhuanrong Wu, Shumao Wang, Hua Han, Zhenbang Xie, Yixuan Wang, Tzu-Hsing Ko

“…Based on the TGA/DTG analysis, the intermediates of cerium chloride hydrates (CeCl3.4H2O and CeCl3.H2O) and cerium anhydrous (CeCl3) were produced, and the formation temperature of CeO2 was estimated to be 773 K. The cubic fluorite structure of CeO2 was detected to be the predominant species and was completely formed at the calcination temperature of 773K–1073 K with a crystal size between 8.8 and 11.4 nm based on the XRD measurement. …”
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Geometrical Features of Subbasal Corneal Whorl-like Nerve Patterns in Dry Eye Disease by Ziqing Feng, MD, Kang Yu, MD, Yupei Chen, MS, Gengyuan Wang, MS, Yuqing Deng, MD, Wei Wang, MD, Ruiwen Xu, MD, Yimin Zhang, MD, Peng Xiao, PhD, Jin Yuan, MD, PhD

“…Methods: All subjects underwent comprehensive ophthalmic examinations, dry eye tests, and in vivo confocal microscopy to visualize the whorl-like corneal nerve at the inferior whorl (IW) region and the straight nerve at the central cornea. The structure of the corneal nerve was extracted and characterized using the fractal dimension (CNDf), multifractal dimension (CND0), tortuosity (CNTor), fiber length (CNFL), and numbers of branching points. …”
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Effects of adding a kind of compound bio-enzyme to the diet on the production performance, serum immunity, and intestinal health of Pekin ducks by Yuxiao Li, Jie Zhou, Tong Guo, Huiya Zhang, Chang Cao, Yingjie Cai, Jiqiao Zhang, Tao Li, Jianqin Zhang

“…In conclusion, this bio-enzyme can improve production performance, intestinal wall structure, and microbiota in Pekin ducks. A 0.1 % concentration of this bio-enzyme is optimal for Pekin duck production.…”
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Bis(7)-harmine derivatives as potential multi-target anti-Alzheimer agents by Hongtao Du, Hongtao Du, Hongtao Du, Fang Ma, Fang Ma, Yuanyuan Cao, Miaoyan Bai, Xinyi Gao, Ziyi Yang, Yang Xu, Yan Yan, Yan Yan

“…In this study, a series of bis(7)-harmine derivatives were designed and synthesized as multifunctional drugs for the treatment of AD.MethodsThe derivatives were synthesized by chemical methods and their structure was confirmed by nuclear magnetic resonance (NMR). …”
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Sandpiper Optimization Algorithm With Region Growing Based Robust Retinal Blood Vessel Segmentation Approach by Ibrahim AlMohimeed, Mohamed Yacin Sikkandar, A. Mohanarathinam, Velmurugan Subbiah Parvathy, Mohamad Khairi Ishak, Faten Khalid Karim, Samih M. Mostafa

“…A wide-ranging experimental analysis is executed and the outcomes are examined on three benchmark databases such as Digital Retinal Images for Vessel Extraction (DRIVE), Structured Analysis of the Retina (STARE), and CHASE&#x005F;DB1 (CHASE). …”
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Synthesis and Characterization of Modified Kaolin-Bentonite Composites for Enhanced Fluoride Removal from Drinking Water by Ebenezer Annan, Emmanuel Nyankson, Benjamin Agyei-Tuffour, Stephen Kofi Armah, George Nkrumah-Buandoh, Joanna Aba Modupeh Hodasi, Michael Oteng-Peprah

“…The FTIR analyses of bentonite (3550 cm−1–4000 cm−1) and kaolin (400–1200 cm−1) correspond to the structural hydroxyl groups and water molecules in the interlayer space of bentonites and the vibrational modes of SiO4 tetrahedron of kaolin, respectively. …”
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Predicting risk factors for Epstein-Barr virus reactivation using Bayesian network analysis: a population-based study of high-risk areas for nasopharyngeal cancer by Zhiwen Zeng, Kena Lin, Xueqi Li, Xueqi Li, Tong Li, Xiaoman Li, Jiayi Li, Zule Ning, Qinxian Liu, Shanghang Xie, Sumei Cao, Sumei Cao, Jinlin Du

“…This study aimed to investigate the risk factors for EBV reactivation associated with NPC in high-prevalence areas in China using a Bayesian network (BN) model combined with structural equation modeling tools.MethodsThe baseline information for this study was derived from NPC screening data from a population-based prospective cohort in Sihui City, Guangdong Province, China. …”
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Chemical Fingerprints of M Dwarfs: High-resolution Spectroscopy on 31 M Dwarfs with SPIRou by Farbod Jahandar, René Doyon, Étienne Artigau, Neil J. Cook, Charles Cadieux, Jean-François Donati, Nicolas B. Cowan, Ryan Cloutier, Stefan Pelletier, Alan Alves-Brito, Jorge H. C. Martins, Hsien Shang, Andrés Carmona

“…We present elemental abundances for up to 10 different elements, including refractory elements such as Si, Mg, and Fe, which are important for modeling the interior structure of exoplanets. In binary systems, we find an average [Fe/H] of −0.15 ± 0.08 for M dwarfs, marginally lower than the reported metallicity of −0.06 ± 0.18 for the FGK primaries from A. …”
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The efficacy of conservative management of micro-traumatic posterior shoulder instability by Lyn Watson, Prof DocBAppSci(Physio) GradDip, Simon Balster, BPhty(Hon), BSc (Hon), Sarah Warby, PhDB Physio (Hon), Ross Lenssen, BHSc(Physiotherapy), Gregory Hoy, MBBS, FRACS, FAOrthA, FACSP, Shane Barwood, MBBS, FRACS, Jacqui French, BPhysio, Bonnie Kerr, BHth ScMSports Physio, Sam Lawrence, DPT, BAppSc, PGCert, Charlotte Ganderton, PhDB Physio (Hon), Katherine Davis, BHuman Movt (ExSci), MPhysio, Tania Pizzari, PhDB Physio (Hon)

“…A common etiology is a gradual overload of glenohumeral joint structures. The recommend initial treatment for microtraumatic PSI is rehabilitation; however, the evidence to support this recommendation is limited. …”
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Reductive acetogenesis is a dominant process in the ruminant hindgut by Qiushuang Li, Jiabin Huo, Gaofeng Ni, Fan Zhang, Shizhe Zhang, Xiumin Zhang, Rong Wang, Jinzhen Jiao, Zhongtang Yu, Xuanxuan Pu, Yipeng Yue, Emilio M. Ungerfeld, Xiaoli Zhang, Jian Wu, Zhiliang Tan, Chris Greening, Min Wang

“…This gastrointestinal tract comprises a foregut (rumen) and hindgut (cecum and colon), which differ in structures and functions, particularly with respect to feed digestion and fermentation. …”
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Post‐operative incidence of lymphedema after RARP with or without extended pelvic lymph node dissection in a cohort study by Andries Clinckaert, Laura Ysenbaardt, Annabel Bijnens, Charlotte Van Calster, Inge Geraerts, Steven Joniau, Nele Devoogdt, Luc Bijnens, Wouter Everaerts

“…Methods A retrospective cohort study was conducted at a tertiary referral centre between April 2016 and July 2020. Structured electronic case report forms (eCRFs) integrated into the electronic health record system were used to document intraoperative, perioperative and postoperative data. …”
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Study on stress corrosion behavior and failure mechanism of galvanized bolts in complex coal mine environments by Zhe He, Nong Zhang, Zhengzheng Xie, Chao Ma, Changliang Han, Fan Zhang, Huiqing Wang, Saad S. Alarifi

“…Additionally, HIC-sensitive structures (tempered martensite) and typical features of HIC (white spots) were found in the galvanized bolts, providing strong evidence of HIC occurrence during their service in the mine. …”
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Sexual practices and risky sexual behaviours among public secondary school adolescents in a local government area in Ekiti State, Nigeria by Ojo JO, Ipinnimo TM, Alao TA, Alabi OS, Adeojo OM, Ilevbare MO, Olorunfemi BN, Esan FJ, Adejumo JO, Dailo CO, Ubulom KA, Ogbonna KO, Adebayo TO, Mesileya AO, Ajewole OO, Stephen-Douglas SJ, Chukwuonye CVS, Oluwagbemi OD

“…Data was obtained using semi-structured self-administered questionnaires developed by the researchers after consulting several literatures. …”
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Design and Experimental Research on a Chisel-Type Variable Hierarchical Deep Fertilization Device Suitable for Saline–Alkali Soil by Nan Xu, Zhenbo Xin, Jin Yuan, Zenghui Gao, Yu Tian, Chao Xia, Xuemei Liu, Dongwei Wang

“…Drawing on the principles of soil dynamics, this paper meticulously investigates the structures of key components and the operating parameters of the fertilization device. …”
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