The efficacy of core decompression combined with regenerative therapy in early femoral head necrosis: a systematic review and meta-analysis involving 954 subjects by Haiwei Tang, Tingxian Ling, Enze Zhao, Mingke You, Xi Chen, Gang Chen, Kai Zhou, Zongke Zhou

“…Secondary outcomes (VAS, HHS, WOMAC score and Lequesne index) showed improved results when CD was combined with other regenerative agents, such as bone mesenchymal stem cells (BMSCs) and bone morphogenetic proteins (BMPs), etc. …”
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An EED/PRC2‐H19 Loop Regulates Cerebellar Development by Pei‐Pei Liu, Xiao Han, Xiao Li, Shang‐Kun Dai, Ya‐Jie Xu, Lin‐Fei Jiao, Hong‐Zhen Du, Li‐Hua Zhao, Rong‐Feng Li, Zhao‐Qian Teng, Yun‐Gui Yang, Chang‐Mei Liu

“…Here, we show that EED deletion from neural stem cells (NSCs) or cerebellar granule cell progenitors (GCPs) leads to reduced GCPs proliferation, cell death, cerebellar hypoplasia, and motor deficits in mice. …”
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Skeletal progenitor LRP1 deficiency causes severe and persistent skeletal defects with Wnt pathway dysregulation by Mohammad Alhashmi, Abdulrahman M. E. Gremida, Santosh K. Maharana, Marco Antonaci, Amy Kerr, Shijian Fu, Sharna Lunn, David A. Turner, Noor A. Al-Maslamani, Ke Liu, Maria M. Meschis, Hazel Sutherland, Peter Wilson, Peter Clegg, Grant N. Wheeler, Robert J. van ‘t Hof, George Bou-Gharios, Kazuhiro Yamamoto

“…Lrp1 deficiency in these stem cells causes joint fusion, malformation of cartilage/bone template and markedly delayed or lack of primary ossification. …”
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A dual role of Cohesin in DNA DSB repair by Michael Fedkenheuer, Yafang Shang, Seolkyoung Jung, Kevin Fedkenheuer, Solji Park, Davide Mazza, Robin Sebastian, Hiroyuki Nagashima, Dali Zong, Hua Tan, Sushil Kumar Jaiswal, Haiqing Fu, Anthony Cruz, Supriya V. Vartak, Jan Wisniewski, Vittorio Sartorelli, John J. O’Shea, Laura Elnitski, Andre Nussenzweig, Mirit I. Aladjem, Fei-Long Meng, Rafael Casellas

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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis by Sayan Deb Dutta, Jeong Man An, Jin Hexiu, Aayushi Randhawa, Keya Ganguly, Tejal V. Patil, Thavasyappan Thambi, Jangho Kim, Yong-kyu Lee, Ki-Taek Lim

“…Transcriptomic (RNA-Seq) and real-time PCR (qRT-PCR) study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase (MAPK) signaling pathway after 7 days. …”
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Genome-wide transcriptome analysis and drug target discovery reveal key genes and pathways in thyroid cancer metastasis by Minjing Zou, Amal Qattan, Monther Al-Alwan, Hazem Ghebeh, Naif Binjumah, Latifa Al-Haj, Khalid S. A. Khabar, Abdulmohsen Altaweel, Falah Almohanna, Abdullah M. Assiri, Abdelilah Aboussekhra, Ali S. Alzahrani, Ali S. Alzahrani, Yufei Shi

“…Mertk, a member of the TAM (Tyro, Axl, Mertk) family of RTKs, was also overexpressed in Met cells, which led to increased MAPK activation, epithelial–mesenchymal transition (EMT), and enrichment of cancer stem cells. Braf-mutant Met cells developed resistance to BRAFV600E inhibitor PLX4720, but remained sensitive to β-catenin inhibitor PKF118-310.ConclusionWe have identified several overexpressed genes/pathways in thyroid cancer metastasis, making them attractive therapeutic targets. …”
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Deficiency of FABP7 Triggers Premature Neural Differentiation in Idiopathic Normocephalic Autism Organoids by Xiao Han, Yuanlin He, Yuanhao Wang, Wenzhu Hu, Chu Chu, Lei Huang, Yuan Hong, Lu Han, Xu Zhang, Yao Gao, Yuan Lin, Hongxia Ma, Hongbing Shen, Xiaoyan Ke, Yan Liu, Zhibin Hu

“…Here, it is generated induced pluripotent stem cell (iPSC)‐derived cerebral organoids from normocephalic individuals with ASD in a prospective birth cohort with a shared clinical diagnosis. …”
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Characteristic of the active substance of the Saccharomyces cerevisiae preparation having radioprotective properties by G. S. Ritter, V. P. Nikolin, N. A. Popova, A. S. Proskurina, P. E. Kisaretova, O. S. Taranov, T. D. Dubatolova, E. V. Dolgova, E. A. Potter, S. S. Kirikovich, Y. R. Efremov, S. I. Bayborodin, M. V. Romanenko, M. I. Meschaninova, A. G. Venyaminova, N. A. Kolchanov, S. S. Bogachev

“…It is supposed that the radioprotective effect of double-stranded RNA is associated with the participation of RNA molecules in the correct repair of radiation-damaged chromatin in blood stem cells. The hematopoietic pluripotent cells that have survived migrate to the periphery, reach the spleen and actively proliferate. …”
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PATHOLOGICAL CHANGES IN MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS DNA by E. V. Dolgova, V. P. Nikolin, N. A. Popova, A. S. Proskurina, K. E. Orishchenko, E. A. Alyamkina, Ya. R. Efremov, S. I. Baiborodin, E. R. Chernykh, A. A. Ostanin, S. S. Bogachev, T. S. Gvozdeva, E. M. Malkova, O. S. Taranov, V. A. Rogachev, A. S. Panov, S. N. Zagrebelnyi, M. A. Shurdov

“…However, the relative amount of CD34+ hematopoietic stem cells (HSCs) from day 15 and till the end of the observation constituted 1,2–1,4 %, which corresponds to the wild-type range. …”
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Identification of glutamine as a potential therapeutic target in dry eye disease by Xiaoniao Chen, Chuyue Zhang, Fei Peng, Lingling Wu, Deyi Zhuo, Liqiang Wang, Min Zhang, Zhaohui Li, Lei Tian, Ying Jie, Yifei Huang, Xinji Yang, Xiaoqi Li, Fengyang Lei, Yu Cheng

“…Our study demonstrated that co-treatment with mesenchymal stem cells (MSCs) and thymosin beta-4 (Tβ4) yielded the best therapeutic outcome against dry eye, surpassing monotherapy outcomes. …”
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B7-H3 CAR-T cell therapy combined with irradiation is effective in targeting bulk and radiation-resistant chordoma cancer cells by Kun Wang, Gianpietro Dotti, Soldano Ferrone, Yufeng Wang, Yin P Hung, Tao Ma, Giulia Cattaneo, Xinhui Wang, David O Osei-Hwedieh, Tara A Walhart, Joseph H Schwab

“…This study explores the efficacy of B7-H3 chimeric antigen receptor T (CAR-T) therapy in vitro and in vivo.Methods Chordoma cancer stem cells (CCSCs) were identified using flow cytometry, sphere formation, and western blot analysis. …”
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Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and a... by Zhongnan Wu, Chong Li, Junhao Li, Tanggan Wang, Meifeng Li, Leyi Zhao, Huimei Ye, Jiaheng Chen, Jiajia Zan, Lijun Song, Qian Zhang, Shaojie Zhang

“…In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC.…”
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In-vitro and in-vivo assessment of biocompatibility and efficacy of ostrich eggshell membrane combined with platelet-rich plasma in Achilles tendon regeneration by Omid Koohi-Hosseinabadi, Reza Shahriarirad, Amireza Dehghanian, Laleh Amini, Sajjad Barzegar, Afrooz Daneshparvar, Omid Alavi, Seyedeh-Parvin Khazraei, Saba Hosseini, Ali Arabi Monfared, Roya Khorram, Nader Tanideh, Soheil Ashkani-Esfahani

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Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration by You Wu, Xiaokun Yue, Ying Zhang, Ning Yu, Chengyan Ge, Rui Liu, Zhongying Duan, Lilong Gao, Xinlong Zang, Xin Sun, Deteng Zhang

“…., bone marrow mesenchymal stem cells, macrophages, Schwann cells, and endothelial cells) cultured on PLCL films tended to align along the stripes and migrate from the periphery toward the center region in vitro. …”
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Ganoboninketal C from Ganoderma boninense improves the efficacy of CDDP-based chemotherapy through inhibiting translesion DNA synthesis by Xiaolu Ma, Fei Yang, Ke Ma, Hongyan Shen, Junjie Han, Kai Wang, Yeran Yang, Jiawei Zhu, Ruiyuan An, Qilin Wang, Tie-Shan Tang, Bo Zhou, Hongwei Liu, Caixia Guo

“…Moreover, 26-3 can significantly sensitize tumor cells to CDDP killing and reduce the proportion of cancer stem cells in AGS and promote apoptosis after CDDP exposure. …”
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Cholangiocyte organoids for disease, cancer, and regenerative medicine by Munemasa Nagao, Akihisa Fukuda, Hirotaka Kashima, Sho Matsuyama, Kei Iimori, Shinnosuke Nakayama, Kenta Mizukoshi, Munenori Kawai, Go Yamakawa, Mayuki Omatsu, Mio Namikawa, Tomonori Masuda, Yukiko Hiramatsu, Yu Muta, Takahisa Maruno, Yuki Nakanishi, Tatsuaki Tsuruyama, Hiroshi Seno

“…With advancements in 3D culture technology, cholangiocyte organoids (COs) derived from primary tissues or induced pluripotent stem cells (iPSCs) can accurately replicate the structural and functional properties of biliary tissues. …”
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Vaccine-induced T cell receptor T cell therapy targeting a glioblastoma stemness antigen by Yu-Chan Chih, Amelie C. Dietsch, Philipp Koopmann, Xiujian Ma, Dennis A. Agardy, Binghao Zhao, Alice De Roia, Alexandros Kourtesakis, Michael Kilian, Christopher Krämer, Abigail K. Suwala, Miriam Stenzinger, Halvard Boenig, Agnieszka Blum, Victor Murcia Pienkowski, Kuralay Aman, Jonas P. Becker, Henrike Feldmann, Theresa Bunse, Richard Harbottle, Angelika B. Riemer, Hai-Kun Liu, Nima Etminan, Felix Sahm, Miriam Ratliff, Wolfgang Wick, Michael Platten, Edward W. Green, Lukas Bunse

“…PTPRZ1-TCR-T maintain stem cell memory phenotype in vitro and in vivo and lyse all examined HLA-A*02+ primary glioblastoma cell lines with a preference for GSCs and astrocyte-like cells. …”
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NPRL2 gene therapy induces effective antitumor immunity in KRAS/STK11 mutant anti-PD1 resistant metastatic non-small cell lung cancer (NSCLC) in a humanized mouse model by Ismail M Meraz, Mourad Majidi, Renduo Song, Feng Meng, Lihui Gao, Qi Wang, Jing Wang, Elizabeth J Shpall, Jack A Roth

“…Humanized-mice were generated by transplanting fresh human cord blood-derived CD34 stem cells into sub-lethally irradiated NSG mice. Lung-metastases were developed from KRAS/STK11mt/aPD1R A549 cells and treated with NPRL2 w/wo pembrolizumab. …”
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Effectiveness of a Novel Liposomal Methylglyoxal–Tobramycin Formulation in Reducing Biofilm Formation and Bacterial Adhesion by Wed Alluhaim, Manal M. Alkhulaifi, Raghad R. Alzahrani, Bahauddeen M. Alrfaei, Alaa Eldeen B. Yassin, Majed F. Alghoribi, Ahlam M. Alsaadi, Ahmed I. Al-Asmari, Ahmed J. Al-Fahad, Rizwan Ali, Naif M. Alhawiti, Majed A. Halwani

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High levels of histone acetylation modifications promote the formation of PGCs by Ziduo Zhao, Yuhui Wu, Fufu Cheng, Zhe Wang, Qingqing Geng, Yingjie Niu, Qisheng Zuo, Yani Zhang

“…This study investigates the role of histone acetylation in the differentiation of chicken embryonic stem cells (ESCs) into primordial germ cells (PGCs). …”
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