Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells by John D. Blair, Austin Hartman, Fides Zenk, Philipp Wahle, Giovanna Brancati, Carol Dalgarno, Barbara Treutlein, Rahul Satija

“…We apply our workflow to cell lines, induced pluripotent stem cells, and months-old retinal and brain organoids to demonstrate its broad applicability. …”
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The Role of the Pathoanatomical Diagnosis «Chronic Gastritis» in the System of Personified Cancer Prevention by A. V. Kononov

“…The activity of gastritis (infiltration of the mucosa with neutrophilic leukocytes) of any etiology is the theoretical justification for the use of bismuth tripotassium dicitrate pharmacological preparation to protect the genome of stem cells of the gastric epithelium as a secondary prevention of gastric cancer.…”
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A biallelically active embryonic enhancer dictates GNAS imprinting through allele-specific conformations by Yorihiro Iwasaki, Monica Reyes, Harald Jüppner, Murat Bastepe

“…Here, we show that the STX16-ICR forms different chromatin conformations with each GNAS parental allele and enhances two GNAS promoters in human embryonic stem cells. When these cells differentiate toward proximal renal tubule cells, STX16-ICR loses its effect, accompanied by a transition to a somatic cell-specific GNAS imprinting status. …”
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Pharmacological activity of OST-01, a natural product from baccharis coridifolia, on breast cancer cells by HyunJun Kang, Dinh Hoa Hoang, Melissa Valerio, Khyatiben Pathak, William Graff, Alexis LeVee, Jun Wu, Mark A. LaBarge, David Frankhouser, Russell C. Rockne, Patrick Pirrotte, Bin Zhang, Joanne Mortimer, Le Xuan Truong Nguyen, Guido Marcucci

“…A shift from a basal-mesenchymal to a luminal-epithelial state of breast cancer stem cells (BCSCs) as supported by the downregulation of stemness (e.g., CD44) and mesenchymal (e.g., FN1 and vimentin) markers, along with the upregulation of differentiation markers (e.g., CD24) and luminal-epithelial markers (e.g., CK19), was also observed.…”
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Analysis of toxicity and mechanisms of busulfan in non-obstructive azoospermia: A genomic and toxicological approach integrating molecular docking, single-cell sequencing, and expe... by Yanggang Hong, Qichao Yuan, Yi Wang, Deqi Wang, Xiaoju Guan, Congde Chen

“…Busulfan, an alkylating chemotherapeutic agent widely used to treat chronic myelogenous leukemia, is known to induce NOA through its toxic effects on spermatogonial stem cells (SSCs). This study aimed to identify key molecular targets and pathways disrupted by busulfan in the testicular environment. …”
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Chitosan suitability to adhesion, neuronal differentiation and maturation of hiPSCs compared to Matrigel coated substrates by Sara Grasselli, Donatella Di Lisa, Pier Francesco Ferrari, Sergio Martinoia, Laura Pastorino

“…Human induced pluripotent stem cells (hiPSCs) derived neurons are revolutionising the study of patient-specific conditions. …”
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Intercellular communication is crucial in the regulation of healthy aging via exosomes by Huifang Sun, Tengyuan Xia, Shuting Ma, Tao Lv, Yuhong Li

“…The hallmarks of aging encompass a variety of molecular categories (genomic, telomeric, and epigenetic), organelles (proteostasis, autophagy, and mitochondria), cellular components (including stem cells), systems (such as intercellular communication and chronic inflammation), and environmental factors (dysbiosis and nutrient sensing). …”
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Sustained slow-release TGF-β3 in a three-dimensional-printed titanium microporous scaffold composite system promotes ligament-to-bone healing by Liwei Zhu, Yuzhe Liu, Yifu Sun, Zhenjia Che, Youbin Li, Tengyue Liu, Xudong Li, Chengzhe Yang, Lanfeng Huang

“…In vitro, the composite system exhibited good biocompatibility and was beneficial for the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs), which showed high growth activity. …”
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Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model by Atsushi Mima, Azuma Kimura, Ryo Ito, Yu Hatano, Hiraku Tsujimoto, Shin-Ichi Mae, Junko Yamane, Wataru Fujibuchi, Norimitsu Uza, Taro Toyoda, Hiroshi Seno, Kenji Osafune

“…Pancreatic acinar cells differentiated from human induced pluripotent stem cells (hiPSCs) have the potential to solve this issue. …”
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Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis by Mahdis Morshedbak, Kaveh Rahimi, Mohammad Reza Tabandeh

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Impact of developmental state, p53 status, and interferon signaling on glioblastoma cell response to radiation and temozolomide treatment. by Artem Berezovsky, Oluwademilade Nuga, Indrani Datta, Kimberly Bergman, Thais Sabedot, Katherine Gurdziel, Susan Irtenkauf, Laura Hasselbach, Yuling Meng, Claudius Mueller, Emanuel F Petricoin, Stephen Brown, Neeraja Purandare, Sidhesh Aras, Tom Mikkelsen, Laila Poisson, Houtan Noushmehr, Douglas Ruden, Ana C deCarvalho

“…In this study, we employed an integrative multi-omics approach, including targeted proteomics, transcriptomics, genomics, and DNA methylation profiling, to investigate the response of a representative panel of GBM patient-derived cancer stem cells (CSCs) to astrocytic differentiation and RT and TMZ treatments. …”
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Differences in phenotypes of normal and malignant cells concerning chromosomal fragility and ganglioside expression by Iskra Sainova, Vera Kolyovska, Desislava Drenska, Dimitar Maslarov, Dimitrina Dimitrova-Dikanarova, Tzvetanka Markova

“…Additionally, the presence and expression of the tumor-suppressor gene HACE-1 in the genome of mouse embryonic stem cells (mESCs) and malignant human cervical carcinoma HeLa cells, both containing an additional copy of this gene, inserted by transfection with appropriate recombinant DNA vectors containing a copy of the tumor-suppressor gene, were tested. …”
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Immune cell profiles and predictive modeling in osteoporotic vertebral fractures using XGBoost machine learning algorithms by Yi-Chou Chen, Hui-Chen Su, Shih-Ming Huang, Ching-Hsiao Yu, Jen-Huei Chang, Yi-Lin Chiu

“…The XGBoost model identified Th1 cells, CD4 memory T cells, and hematopoietic stem cells as key predictors of VF. Notably, VF patients exhibited a reduction in Th1 cells and an enrichment of Th17 cells, which promote osteoclastogenesis and bone resorption. …”
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EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL by Brilliant Citra Wirashada, Dwikora Novemberi Utomo, Lukas Widhiyanto

“…Rabbits were divided into 4 treatment groups: microfracture defect group with DFLP sponge cartilage scaffold (P1) implantation; Microfracture defect group with DFLP sponge cartilage scaffold-secretome implantation (P2); Microfracture defect group with DFLP sponge cartilage scaffold-adipose derived Mesenchymal Stem Cells (ADMSCs) (P3); Microfracture defect group without implantation (control). …”
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A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection. by Emily A Fitzmeyer, Taru S Dutt, Silvain Pinaud, Barb Graham, Emily N Gallichotte, Jessica L Hill, Corey L Campbell, Hunter Ogg, Virginia Howick, Mara K N Lawniczak, Erin Osborne Nishimura, Sarah Hélène Merkling, Marcela Henao-Tamayo, Gregory D Ebel

“…In contrast, proliferating intestinal stem cells (ISC) had the lowest levels of vRNA, a finding consistent with studies suggesting ISC proliferation in the midgut is involved in infection control. …”
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Long-term correction of hemophilia A via integration of a functionally enhanced FVIII gene into the AAVS1 locus by nickase in patient-derived iPSCs by Do-Hun Kim, Sang-Hwi Choi, Jin Jea Sung, Sieun Kim, Hanui Yi, Sanghyun Park, Chan Wook Park, Young Woo Oh, Jungil Lee, Dae-Sung Kim, Jong-Hoon Kim, Chul-Yong Park, Dong-Wook Kim

“…Genome editing in conjunction with patient-derived induced pluripotent stem cells (iPSCs) is a promising cell therapy strategy, as it replaces dysfunctional proteins resulting from genetic mutations with normal proteins. …”
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The guanine nucleotide exchange factor DOCK5 negatively regulates osteoblast differentiation and BMP2-induced bone regeneration via the MKK3/6 and p38 signaling pathways by Ju Ang Kim, Soomin Im, Jiwon Lim, Jung Min Hong, Hye Jung Ihn, Jong-Sup Bae, Jung-Eun Kim, Yong Chul Bae, Eui Kyun Park

“…Osteoblast differentiation and bone regeneration were analyzed using bone marrow mesenchymal stem cells (BMSCs) and various animal models. C21 significantly enhanced osteoblast differentiation and mineral deposition in mouse MC3T3-E1 cells and in human and mouse BMSCs. …”
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Psoralidin: emerging biological activities of therapeutic benefits and its potential utility in cervical cancer by Tanya Tripathi, Apoorva Chaudhary, Divya Janjua, Udit Joshi, Nikita Aggarwal, Chetkar Chandra Keshavam, Alok Chandra Bharti

“…Its capacity to effectively target cancer stem cells (CSCs) in general adds to its therapeutic potential. …”
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Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis by Wei Shi, Dong Li, Qian Xu, Kai Zhang, Xinyu Liang, Hui Li, Zhijun Li, Huafeng Zhang

“…Results After integrating the 9 samples, 14 cell types were identified: B cells, Mesenchymal stem cells, Osteoblasts, Endothelial cells, Monocytes, T cells, NK cells, Fibroblasts, Macrophages, Common myeloid progenitors, Chondrocytes, Myelocytes, Osteoclasts, and Pericytes. …”
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Timosin alpha-1 immunomodulating properties by M. V. Mayevskaya

“…Immunomodulating effect of Timosin alpha-1 consists in increase of natural killer cells number, CD4 and CD8 by stimulation of stem cells differentiation and decrease of T-cells apoptosis, and also in driving of immune response to Th1 subtype by increase of Th1 cytokines (IL-2, IFN) amount and decreases of amount of Th2 cytokines (IL-4, IL-10). …”
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