Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice by Yali Fu, Ting Ye, Ming Chen, Bin Lai, Ping Zheng

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Protocol for fecal microbiota transplantation: A microaerophilic approach for mice housed in a specific pathogen-free facility by Shari Wouters, Hugo Moors, Mieke Verslegers, Natalie Leys, Surbhi Malhotra-Kumar, Samir Kumar-Singh, Mohamed Mysara

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Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans by Olivia M. Osborne, Oandy Naranjo, Silvia Torices, Sarah Schmidlin, Destiny Tiburcio, Minseon Park, Michal Toborek

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Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics by Yan Ma, Keshu Dong, Jie Hu, Yiyun Tang, Hanfu Xu

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Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure by Ranhao Zhang, Yuan Shen, Xueming Li

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Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays by Stefano Conti Nibali, Andrea Magrì, Angela Messina, Armin Wagner, Ramona Duman, Vito De Pinto, Cristian Turato, Cristina Arrigoni, Marco Lolicato

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Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique by Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya

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Protocol to assess changes in brain network resistance to perturbation during offline processing using TMS-EEG by Martina Bracco, Tuomas P. Mutanen, Domenica Veniero, Gregor Thut, Edwin M. Robertson

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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

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Protocol for live imaging of axonal transport in iPSC-derived iNeurons by Dan Dou, Erika L.F. Holzbaur, C. Alexander Boecker

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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

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Protocol for cell image-based spatiotemporal proteomics in budding yeast by Athanasios Litsios, Myra Paz David Masinas, Helena Friesen, Charles Boone, Brenda J. Andrews

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Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time by Amy Llewellyn, Rachel Barrow-McGee, Julia Stevenson, Jasmine Gore, Kalnisha Naidoo

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4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos by Martin H. Dominguez, Jonathon M. Muncie-Vasic, Benoit G. Bruneau

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Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells by Linlin Li, Jun Jin

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Protocol to detect neutral lipids with BODIPY staining in myeloid-derived suppressor cells in mouse mammary tumors by Ukjin Kim, Rumela Chakrabarti

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Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei by Lei Li, May G. Akl, Scott B. Widenmaier

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Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment by Kenta Takeuchi, Shunsuke Tabe, Yuya Yamamoto, Kenta Takahashi, Megumi Matsuo, Yasuharu Ueno, Masayuki Ohtsuka, Soichiro Morinaga, Yohei Miyagi, Tomoyuki Yamaguchi, Naoki Tanimizu, Hideki Taniguchi

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Protocol for the three-dimensional analysis of rodent skeletal muscle by Smrithi Karthikeyan, Yoko Asakura, Mayank Verma, Atsushi Asakura

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