Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique by Alexander Brückner, Adrian Brandtner, Sarah Rieck, Bernd K. Fleischmann, Daniela Wenzel

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Protocol for CRISPR-based endogenous protein tagging in mammalian cells by Yu-Xi Xiao, Jiarun Wei, Jason Moffat

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Protocol for capturing a full transcriptome from single preimplantation embryos using So-Smart-seq by Chunyao Wei, Jeannie T. Lee

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Protocol to study the impact of breast cancer on colonization resistance of mouse microbiota using network node manipulation by Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Apolline Maitre, Janet Avellanet, Arlem García, Dasha Fuentes, Alejandro Cabezas-Cruz

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Protocol for assessing the clogging of the mitochondrial translocase of the outer membrane by precursor proteins in human cells by John Kim, Hilla Weidberg

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Protocol for predicting host-microbe interactions and their downstream effect on host cells using MicrobioLink by Lejla Gul, Anna Julia Elias, Tanvi Tambaku, Marton Olbei, Emily Watters, Balazs Bohar, Dezso Modos, Matthew Madgwick, Tamas Korcsmaros

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Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software by Pieter Both, Soochi Kim, Jengmin Kang, Marina Arjona, Daniel I. Benjamin, Christopher W. Nutter, Armon Goshayeshi, Thomas A. Rando

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Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms by Hongyu Xu, Zetian Jia, Junhui Liu, Runming Liu, Wei Wei, Xiang Li

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Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory viruses by Victor H.K. Lam, Aleena Ghafoor, Yazan Khan, Shirley Constable, Lane B. Buchanan, David Zuanazzi, Reeya Parmar, Zeynep G. Tepe, Leigh J Sowerby, Cindy M. Liu, Ryan M. Troyer, Jessica L. Prodger

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Protocol to generate a 3D atherogenesis-on-chip model for studying endothelial-macrophage crosstalk in atherogenesis by Katie M.L. Hanford, Kim E. Dzobo, Miranda Versloot, Jorge Peter, Jeffrey Kroon

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Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice by Kyohei Tokizane, Shin-ichiro Imai

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Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model by Shuyi Liu, Wei Si, Zhengbo Wang

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Protocols for translocation processes of flexible polymers through a pore using LAMMPS by Vrinda Garg, Rejoy Mathew, Arindam Chatterjee, Surya K. Ghosh

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Protocol to study toxic, immune, and suicidal phenotypes of killer yeast by Rianne C. Prins, Sonja Billerbeck

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Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

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Protocol for semisynthesis of histone H4 with site-specific modifications using irreversible sortase-mediated ligation by Yihang Xiao, Kun Zou, Mingxuan Wu

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Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism by Martina Dzubanova, Michaela Ferencakova, Andrea Benova, Dalia Ali, Michaela Tencerova

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Comprehensive guide for epigenetics and transcriptomics data quality control by Arianna Comendul, Frederique Ruf-Zamojski, Colby T. Ford, Pankaj Agarwal, Elena Zaslavsky, German Nudelman, Manoj Hariharan, Aliza Rubenstein, Hanna Pincas, Venugopalan D. Nair, Adam M. Michaleas, Philip D. Fremont-Smith, Darrell O. Ricke, Stuart C. Sealfon, Christopher W. Woods, Kajal T. Claypool, Rafael Jaimes, III

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Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq by Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li

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Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses by Karim Abu Nahia, Cecilia Lanny Winata

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