Protocol for 3D photogrammetry and morphological digitization of complex skulls by Naomi De Leo, Claudio Chimenti, Luigi Maiorano, Davide Tamagnini

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Protocol for functional screening of CFTR-targeted genetic therapies in patient-derived organoids using DETECTOR deep-learning-based analysis by Mattijs Bulcaen, Ronald B. Liu, Kasper Gryspeert, Sam Thierie, Anabela S. Ramalho, François Vermeulen, Xavier Casadevall I Solvas, Marianne S. Carlon

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Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack by Atanu Pramanik, Shubham Nema, Yogesh Bhargava, Anamika Bhargava

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Protocol for predicting the single-cell network-based gene activity landscape during human B cell development by Xin Huang, Xuetong Hou, Yizhen Li, Jun J. Yang, Jiyang Yu

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Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology by Vineeth A. Raveendran, Jessica C. Pressey, Melanie A. Woodin

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Protocol for isolating and purifying murine liver sinusoidal endothelial cells for in vitro culture and functional assays by Sophia Papaioannou, Jia-Xiang See, Tinja Baljkas, Philipp Reiners-Koch, Manuel Winkler, Adelheid Cerwenka, Ana Stojanovic

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Protocol to identify small-molecule inhibitors against cancer drug resistance by Juan Wei, She-Yu Zhang, Xue-Yuan Zhou, Yong Wei, Hao-Ran Jia, Qin Wu, Weihong Tan

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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

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Protocol for generating protein profiles and distance-based network analysis of murine tissue slices by Luisa Schmidt, Philipp Antczak, Marcus Krüger

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Protocol to denoise spatially resolved transcriptomics data utilizing optimal transport-based gene filtering algorithm by Lin Du, Jingmin Kang, Jie Li, Hua Qin, Yong Hou, Hai-Xi Sun

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Protocol to identify endogenous proximal proteins using biotinylation by antibody recognition by Camilla Rega, Mercedes Pardo, Lesley-Ann Martin, Jyoti Choudhary

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Protocol for measuring the auxin-induced changes of m6A modification by Bin Li, Yixing Li, Lei Li, Linjun Cai, Lan Li, Li Li, Chongsheng He

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Protocol for the purification and analysis of nuclear UFMylated proteins by Pudchalaluck Panichnantakul, Marlene Oeffinger

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Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model by Shinobu Nakanishi

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Protocol for identifying Mycobacterium tuberculosis infection status through airway microbiome profiling by Geoffrey Olweny, Moses Levi Ntayi, Edward Kyalo, Alex Kayongo

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Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells by Kenji Miki, Mikako Yagi, Ko Igami, Hiroki Kittaka, Akito Tani, Natsuki Horiuchi, Satoshi Fukumoto, Koji Yoshimoto, Takeshi Uchiumi

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Computational protocol for analyzing whole-genome sequencing data from Staphylococcus aureus clinical isolates by Miquel Sánchez-Osuna, Ivan Erill, Oriol Gasch, Oscar Q. Pich

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Protocol for visualizing the chromatin assembly properties of epigenetic protein complexes via an HTM module-mediated artificial tethering system by Shanli Guan, Jiajia Tang, Cuixia Di, Bo Cheng

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Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells by Zhongjun Wan, Shanshan Wen, Ran Zheng, Li Li, Wei Jiang, Donghui Zhang

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Protocol for evaluating the activity of R2 retrotransposons in mammalian cells by Yangcan Chen, Shengqiu Luo, Yanping Hu, Qi Zhou, Wei Li

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