重组人高密度脂蛋白B族I型清道夫受体基因腺相关病毒血 清型2/1杂合载体的构建及其在HepG2肝细胞的表达

“…【目的】 构建含绿色荧光蛋白(EGFP)重组人高密度脂蛋白B族I型清道夫受体(SR-BI)基因腺相关病毒(rAAV)2/1杂合载体,观察其在HepG2肝细胞的表达65377;【方法】以pCMV.SPORT6-SR-BI质粒为模板PCR扩增目的基因cDNA,将其克隆至pSNAV2.0-IRES-EGFP-LacZa载体中,经酶切及测序鉴定后,脂质体介导转染BHK21细胞,经G418筛选得到含SR-BI基因的BHK/SR-BI-IRES-EGFP载体细胞株,用携带rep2-cap1基因的辅助病毒(rHsv/r2cl)感染该细胞株65377;通过细胞孵育65380;裂解和病毒纯化,得到杂合载体rAAV2/1-SR-BI-IRES-EGFP病毒65377;免疫荧光显微镜检测绿色荧光表达,计算转染效率,Western Blot检测转染后SR-BI蛋白的表达65377; 【结果】 酶切鉴定表明pSNAV2.0-SR-BI-IRES-EGFP重组成功,基因测序显示装入pSNAV2.0-IRES-EGFP-LacZa质粒中的SR-BI基因正确;成功构建了rAAV2/1-SR-BI-IRES-EGFP病毒载体65377;转染HepG2肝细胞后免疫荧光显微镜检测最佳MOI值为1 × 105时, 以此值转染HepG2肝细胞荧光高效表达; Western Blot检测未转染组及rAAV2/1-IRES-EGFP组HepG2肝细胞均有SR-BI蛋白表达,rAAV2/1-SR-BI-IRES-EGFP组SR-BI蛋白表达水平较rAAV2/1-IRES-EGFP组及未转染组显著增加65377;【结论】 成功构建了rAAV2/1-SR-BI-IRES-EGFP杂合载体系统,转染后SR-BI蛋白在HepG2肝细胞高效表达,为SR-BI生理学作用机制及临床应用的深入研究奠定基础65377;…”
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Cross sectional associations of physical activity and sleep with mental health among Chinese university students by Bo Li, Shan-shan Han, Yu-peng Ye, Ya-xing Li, Shu-qiao Meng, Shuo Feng, Han Li, Zhong-lei Cui, Yang-Sheng Zhang, Yao Zhang, Qing Zhang, Guang-xu Wang, Hu Lou, Weidong Zhu, Yang Liu

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Repetitive Transcranial Magnetic Stimulation: Is it an Effective Treatment for Cancer Pain? by Yanyuan Du, Yaoyuan Li, Jieqing Hu, Ruiying Fang, Yuming Liu, Liu Cai, Ying Song, Susu Ma, Jin Gao, Hanyue Zhang, Baihui Li, Hongtai Xiong, Huibo Yu, Shenglei Yang, Shuduo Zhu, Honggang Zheng

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Association of digital screen use during recess with physical activity behaviours in 10-year-old to 17-year-old Danish adolescents: a population-based cross-sectional study by Soren Brage, Jens Troelsen, Anders Grøntved, Peter Lund Kristensen, Jan C Brønd, Anne Kær Gejl, Niels C Møller, Kristian T Larsen, Sofie Koch, Natascha H Pedersen

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Extracellular vesicles contribute to the beneficial effects of exercise training in APP/PS1 mice by Oliver K. Fuller, Emma D. McLennan, Casey L. Egan, Nimna Perera, Lauren V. Terry, Jae Pyun, Mariana de Mendonca, Guilherme Defante Telles, Benoit Smeuninx, Emma L. Burrows, Ghizal Siddiqui, Darren J. Creek, John W. Scott, Michael A. Pearen, Pamali Fonseka, Joseph A. Nicolazzo, Suresh Mathivanan, Anthony J. Hannan, Grant A. Ramm, Martin Whitham, Mark A. Febbraio

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Clinical and radiographic outcomes using standard length of cannulated screws for traditional Latarjet procedure by Juan M. Lopez-Ovenza, MD, Amaury Tapia, MD, Juan I. Duca, MD

“…Functional evaluation was performed with postoperative Rowe and Walch score, visual analog scale for pain and return to sport. Graft healing was assessed with computed tomography scans at 4 to 6 months postoperatively. …”
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Cohort profile: Oxford Pain, Activity and Lifestyle (OPAL) Study, a prospective cohort study of older adults in England by Sarah E Lamb, Christian D Mallen, M Watson, Julie Bruce, S Dutton, S Petrou, Lesley Ward, Maria T Sanchez Santos, Esther Williamson, Angela Garrett, Alana Morris, O Conway, F Darton, D Hagan, D Haywood, A Hewitt, I Marian, L Nevay, P Nicolson, M Slark, K Vadher, E Williamson, N Arden, K Barker, G Collins, J Fairbank, J Fitch, D French, F Griffiths, Z Hanson, C Hutchinson

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Feasibility study to assess the delivery of a lifestyle intervention (TreatWELL) for patients with colorectal cancer undergoing potentially curative treatment by Annie S Anderson, Ronan E O’Carroll, Martine Stead, Robert J C Steele, Mary Wells, Maureen Macleod, Anna Campbell, Jacqui A Sugden, Jackie Rodger, Jennifer McKell

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Optimal vocal therapy for respiratory muscle activation in patients with COPD: effects of loudness, pitch, and vowels by Zhengtong Qiao, Ziwei Kou, Jiazhen Zhang, Daozheng Lv, Dongpan Li, Xuefen Cui, Kai Liu

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Game mechanics in eHealth interventions promoting self-management in young people with chronic diseases: a protocol for a systematic review and meta-analyses from the eHealth Junio... by Sanne L Nijhof, Elise van de Putte, Fernando Estévez-López, Wichor M Bramer, Lisa Levelt, Job van ’t Veer, Micah M Hrehovcsik, Valentijn Visch, Manon H. J. Hillegers, Remco C Veltkamp

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Evaluation of the efficacy of a novel lumbar exoskeleton with multiple interventions for patients with lumbar disc herniation: a multicenter randomized controlled trial of non-infe... by Xiaonan Huang, Xiaonan Huang, Lele Huang, Lele Huang, Lei Shi, Lifan Xu, Lifan Xu, Chengbing Cao, Heng Wu, Min Cao, Can Lv, Ping Shi, Guohui Zhang, Fanfu Fang

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The impact of endolymphatic hydrops on wideband acoustic immittance and otoacoustic emissions in guinea pigs by Huan Lin, Xuanyi Li, Huiying Zhang, Yi Mu, Xi Wang, Naveena Konduru, Renlong Ji, Wen Liu, Wen Liu, Wen Liu, Zhao Fei, Wen Jiang, Wen Jiang, Wen Jiang, Yuehua Qiao, Yuehua Qiao, Yuehua Qiao

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Social capital and physical activity: a literature review up to March 2024 by Zhendong Gao, Chen Soon Chee, Roxana Dev Omar Dev, Yutong Liu, Jianhong Gao, Rui Li, Fangyi Li, Xiaoxiao Liu, Tao Wang

“…This study aims to systematically review the literature up to 2024 on the relationship between social capital and physical activity, uncover the role of social capital in promoting physical activity, and identify its multidimensional impacts.MethodsWe used a combination of search terms including “social capital” and “physical activity” to search the Web of Science, PubMed, Scopus, SportDiscus, and PsychINFO databases for English literature published up to March 1, 2024.ResultsWe identified 2,021 unique articles and reviewed 115 studies that met our inclusion criteria. …”
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The effects of mobile phone addiction on learning engagement of Chinese college students - the mediating role of physical activity and academic self-efficacy by Shuqiao Meng, Kai Qi, Panyi Shen, Meng Zhang, Yu Zhang, Nzubechi Onyebuchi, Ge Zhan, Fanqi Wei, Wenxia Tong, Yahui Han, Xiaoyu Ge

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The impact of COVID-19 pandemic on sleep duration and mental health among middle school students: a 3-year cross-sectional survey by Kai-yun Dou, Ru-bao Dong, Chun-long Gan

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Assessment of stress and health conditions among students in the context of the war in Ukraine by Vladimir Potop, Igor Vypasniak, Iryna Ivanyshyn, Vasyl Lutskyi, Iryna Kryventsova, Lyudmila Shesterova, Katarzyna Prusik

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Supplementation with essential amino acids in the early stage of carbohydrate reintroduction after a very-low energy ketogenic therapy (VLEKT) improves body cell mass, muscle stren... by Giuseppe Annunziata, Ludovica Verde, Vincenzo D’Orsi, Massimiliano Caprio, Stefania Gorini, Silvia Savastano, Annamaria Colao, Giovanna Muscogiuri, Luigi Barrea

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Development of a checklist for cognitive assessment requirements (CARE) based on a Delphi consensus study by Vahid Nejati, Reza Estaji, Vicent Balanzá-Martínez, David A Balota, Suzanne Barker-Collo, Morris Bell, Jacquelyn H Berry, Khatereh Borhani, Munro Cullum, Anthony Feinstein, Charles J Golden, Raul Gonzalez, Jordan Henry Grafman, Steven D Hollon, Petra Jansen, Nicole A Kochan, Ryan Van Patten, Olivier Piguet, Sarah A Raskin, Sean B. Rourke, Andrew Scholey, Yaakov Stern, Steven Paul Woods, Michael I. Posner

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Global, regional, and national trends in drug use disorder mortality rates across 73 countries from 1990 to 2021, with projections up to 2040: a global time-series analysis and mod... by Soeun Kim, Hayeon Lee, Selin Woo, Hyeri Lee, Jaeyu Park, Tae Kim, Guillaume Fond, Laurent Boyer, Masoud Rahmati, Lee Smith, Guillermo F. López Sánchez, Elena Dragioti, Christa J. Nehs, Jinseok Lee, Hyeon Jin Kim, Jiseung Kang, Dong Keon Yon

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Association between selective serotonin reuptake inhibitors use and blood transfusion risk in older adults after hip fracture: a cohort study by Héloïse Gobillot-Morisson, Bastien Genet, Corinne Frère, Judith Cohen-Bittan, Mathieu Raux, Marie-Eva Rollet, Anthony Meziere, Jacques Boddaert, Lorène Zerah, Sara Thietart

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