Proliferating CLL cells express high levels of CXCR4 and CD5 by Daniel Friedman, Drshika P. Mehtani, Jennifer B. Vidler, Piers E. M. Patten, Robbert Hoogeboom

“…In Eµ‐TCL1 transgenic mice, the CXCR4hiCD5hi fraction expressed high levels of ki67 and was expanded in enlarged spleens of diseased animals. Human peripheral blood CXCR4hiCD5hi CLL cells express increased levels of IgM and the chemokine receptors CCR7 and CXCR5 and migrate efficiently toward CCL21. …”
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Intra-Articular Injection of Human Synovial Membrane-Derived Mesenchymal Stem Cells in Murine Collagen-Induced Arthritis: Assessment of Immunomodulatory Capacity In Vivo by Minglu Yan, Xin Liu, Qiujie Dang, He Huang, Fan Yang, Yang Li

“…Flow cytometry showed that Th1 and Th17 cells decreased, whereas Th2, regulatory T (Treg), and PD-1+CXCR5+FoxP3+ follicular Treg cells increased in the spleens of SM-MSC-treated mice. Regulatory B cell analysis showed that CD21hiCD23hi transitional 2 cells, CD23lowCD21hi marginal zone cells, and CD19+CD5+CD1d+IL-10+ regulatory B cells increased following SM-MSC treatment. …”
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Concurrent severe fever with thrombocytopenia syndrome virus outbreaks on multiple fox farms, China, 2023 by Jian Sun, Lei Qian, Delong Li, Xiurong Wang, Hong Zhou, Cixiu Li, Edward C. Holmes, Jianke Wang, Juan Li, Weifeng Shi

“…In autopsy and histopathology, prominently enlarged spleens and extensive multi-organ hemorrhage were observed, respectively, indicating severe systemic effects. …”
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Interleukin-2/Anti-Interleukin-2 Immune Complex Expands Regulatory T Cells and Reduces Angiotensin II-Induced Aortic Stiffening by Beenish Majeed, Supannikar Tawinwung, Lance S. Eberson, Timothy W. Secomb, Nicolas Larmonier, Douglas F. Larson

“…Our results indicate that the IL-2/mAbCD25 complex effectively induced Treg phenotype expansion by 5-fold in the spleens with minimal effects on total CD4+ and CD8+ T-lymphocyte numbers. …”
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Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model by Takeshi Suzuki, Kei Inoue, Toru Igarashi, Jungo Kato, Hiromasa Nagata, Takashige Yamada, Shizuka Minamishima, Hiroshi Morisaki

“…In the first control study, 20 animals were allocated to 4 groups: control group with sham operation (group C, n = 5) and 3 groups with cecum ligation and puncture (CLP) performed at 3 different sites: proximal, middle, and distal cecum (groups CLP-P, CLP-M, and CLP-D, respectively; n = 5 in each group). Their spleens were resected under general anesthesia 24 hours after CLP, and the total number of normal splenic T lymphocytes per mouse and the percentage of apoptotic T lymphocytes were evaluated using flow cytometry. …”
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Therapeutic potential of the neutralizing monoclonal antibody 45G3 against encephalomyocarditis virus by Yanfang Zhang, Zhiying Wang, Yaohui Fang, Qiong Zhu, Jie Fu, Sijing Hu, Jiayin Jin, Min Zhou, Xijia Liu, Danna Zhang, Shouwei Huang, Yali Deng, Lingling Xie, Shu Shen, Jing Ye, Fei Deng, Shengbo Cao

“…In this study, we evaluated the neutralizing effects of a monoclonal antibody derived from the spleens of mice immunized with EMCV virus-like particles (VLPs), both in vitro and in vivo. …”
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Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in... by Liding Zhao, Ya Li, Qingbo Lv, Min Wang, Yi Luan, Jiale Song, Guosheng Fu, Junbo Ge, Yunzeng Zou, Wenbin Zhang

“…In CD11c+ DCs derived from the spleens with hyperglycemia, the expression of CD83 and CD86 in diabetic mice was significantly upregulated, coupled with a higher secretion level of cytokines and chemokines, and increased phosphorylation of NF-κB and IκB. …”
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Regulation of NK cell development, maturation, and antitumor responses by the nuclear receptor NR2F6 by Johannes Woelk, Florian Hornsteiner, Stephanie Aschauer-Wallner, Patrizia Stoitzner, Gottfried Baier, Natascha Hermann-Kleiter

“…Conventional type 1 dendritic cells (cDC1) and macrophage populations are decreased in spleens of Nr2f6-deficient mice, subsequently, IL-15-dependent NK cell priming is limited. …”
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The Role of β-Catenin in Th1 Immune Response against Tuberculosis and Profiles of Expression in Patients with Pulmonary Tuberculosis by Kunlong Xiong, Jinxia Niu, Ruijuan Zheng, Zhonghua Liu, Yanzheng Song, Lin Wang, Changtai Zhu, Lin Fan

“…CD4, CD8, β-catenin, IFN-γ, and TNF-α in mononuclear cells from the lungs and spleens were measured by flow cytometry, and the pathological changes of lungs were also observed. …”
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PARP-1 Is Critical for Recruitment of Dendritic Cells to the Lung in a Mouse Model of Asthma but Dispensable for Their Differentiation and Function by Laura C. Echeverri Tirado, Mohamed A. Ghonim, Jeffrey Wang, Amir A. Al-Khami, Dorota Wyczechowska, Hanh H. Luu, Hogyoung Kim, Maria Dulfary Sanchez-Pino, José Yélamos, Lina M. Yassin, A. Hamid Boulares

“…An assessment of CD11b+/CD11c+/MHCIIhigh DCs in spleens and lymph nodes of OVA-sensitized mice revealed that PARP-1 inhibition genetically or by olaparib exerted little to no effect on DC differentiation, percentage of CD80+/CD86+/CD40+-expressing cells, or their capacity to promote proliferation of ovalbumin-primed (OTII) CD4+ T cells. …”
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Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein by Wang BH, Yu KY, Zhang XN, Sun XH, Tang LL, Shi XL

“…Flow cytometry was used to detect T helper 17 (Th17)/regulatory T (Treg) cells and T helper 1(Th1)/T helper 2 (Th2) lymphocyte ratios and the proportions of conventional myeloid dendritic cells (cDCs), plasma cell-like DCs, CD80 and CD86 cells in mouse spleens.Results: HE and PAS staining showed that, compared to that in the normal group, the lung tissue of the D1 group mice showed a significant inflammatory damage response, whereas the D3 and D10 groups showed a gradual recovery trend. …”
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