Nicotine’s impact on platelet function: insights into hemostasis mechanisms by Xiayu Wu, Xiayu Wu, Yongjun Liu, Changhao Zou, Changhao Zou, Fuqin He, Fuqin He, Fang Guo, Fang Guo, Sijia Liu, Sijia Liu, Yi Fan, Yi Fan, Xuedong Zhu, Xuedong Zhu, Qianyi Zhou, Qianyi Zhou, Dan Shu, Dan Shu

“…Through the examination of alterations in the downstream signaling pathways of PAR4 receptors, it was determined that nicotine promo-facilitates the phosphorylation of PI3K, AKT, and ERK1/2 proteins, subsequently contributing to the activation of αIIbβ3 receptors in platelets. …”
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Comparative Transcriptome Analyses Reveal the Mechanisms Underlying Waterlogging Tolerance in Barley by Juan Zhu, Haoxin Yin, Cong Cao, Chengqun Sun, Mengna Zhang, Yi Hong, Yuhang Zhang, Chao Lv, Baojian Guo, Feifei Wang, Rugen Xu

“…KEGG analysis showed that glycolysis/gluconeogenesis, the MAPK signaling pathway, plant hormone signaling, and galactose metabolism pathways were significantly enriched in the waterlogging-tolerant genotype TamF169 four days after waterlogging. …”
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Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation by Jingju Yin, Jingju Yin, Jingju Yin, Jingju Yin, Yunyang Liao, Yunyang Liao, Yunyang Liao, Yunyang Liao, Shaofeng Liu, Shaofeng Liu, Shaofeng Liu, Shaofeng Liu, Bangwei Che, Hanghang Zhu, Hanghang Zhu, Bingbing Yang, Bingbing Yang, Bingbing Yang, Bingbing Yang, Bin Shi, Bin Shi

“…Magnetic bead sorting of CD3+ T cells and transcriptome sequencing were performed to explore the signaling pathways and key immune factors that may influence the related immune responses.ResultsBioinformatics analysis showed that healthy peri-implant tissues were enriched by the most of T-cell subtypes. …”
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Macrophage Dvl2 deficiency promotes NOD1-Driven pyroptosis and exacerbates inflammatory liver injury by Xiaoye Qu, Dongwei Xu, Tao Yang, Yizhu Tian, Christopher T. King, Xiao Wang, Mingwei Sheng, Yuanbang Lin, Xiyun Bian, Changyong Li, Longfeng Jiang, Qiang Xia, Douglas G. Farmer, Bibo Ke

“…Dishevelled 2 (Dvl2) is a key mediator of the Wingless/Wnt signaling pathway that regulates cell proliferation, migration, and immune function. …”
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Effects of pyrazosulfuron-ethyl on caudal fin regeneration in zebrafish larvae by Chenkai Ge, Zhijun Ye, Weitao Hu, Jingrong Tang, Huimin Li, Fasheng Liu, Xinjun Liao, Jianjun Chen, Shouhua Zhang, Zigang Cao

“…However, inhibiting the Notch signaling pathway and inhibiting reactive oxygen cannot rescue the fin regeneration defects induced by pyrazosulfuron-ethyl. …”
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Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes by Jing Wang, Chenwen Xiao, Shuang Liang, Muhammad Noman, Yingying Cai, Zhen Zhang, Xueming Zhu, Rongyao Chai, Haiping Qiu, Zhongna Hao, Yanli Wang, Jiaoyu Wang, Guolian Bao, Guochang Sun, Fucheng Lin

“…MoCdr1 and TmCdr1 are conserved in structures and functions, and both involved in drug resistance and pathogenicity by analogously regulating gene expression levels related to transporter activity, MAPK signaling pathway, and metabolic processes. Altogether, our results represent the first comprehensive characterization of ABC genes in T. mentagrophytes, establishing a functional correlation between homologous ABC genes in plant and animal pathogenic fungi.…”
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Retinoblastoma-binding Protein 9 Suppresses Intestinal Inflammation and Inflammation-induced Tumorigenesis in MiceSummary by Kensuke Hamada, Yuki Nakanishi, Yu Muta, Mayuki Omatsu, Kosuke Iwane, Munehiro Ikeda, Jiayu Chen, Yoko Masui, Naoki Aoyama, Nobukazu Agatsuma, Go Yamakawa, Takahiro Utsumi, Hiroki Kitamoto, Makoto Okabe, Yoshiro Itatani, Takumi Adachi, Koubun Yasuda, Shuji Yamamoto, Akihisa Fukuda, Etsushi Kuroda, Masaki Ohmuraya, Kazutaka Obama, Seiichi Hirota, Hiroki Ikeuchi, Kenji Nakanishi, Hiroshi Seno

“…Conclusions: RBBP9 suppresses the intestinal inflammation by negatively regulating JAK/STAT1 signaling pathway.…”
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GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) by Mingliang Yang, Mingliang Yang, Chengjun Lei, Chengjun Lei, Chao Ma, Chao Ma, Xiuming Hou, Xiuming Hou, Mingming Yao, Mingming Yao, Liang Mi, Liang Mi, Enliang Liu, Linli Xu, Shukun Wang, Shukun Wang, Chunyan Liu, Chunyan Liu, Qingshan Chen, Qingshan Chen, Dawei Xin, Dawei Xin, Chang Xu, Chang Xu, Jinhui Wang, Jinhui Wang

“…In summary, our research revealed that BR suppresses root nodule formation by modulating the immune signaling pathway in soybean roots. We further identified that GmWRKY33a, a crucial transcription factor in BR signaling, plays a negative role in the symbiotic establishment.…”
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CD40-TRAF6 inhibition suppresses cardiovascular inflammation, oxidative stress and functional complications in a mouse model of arterial hypertension by Lea Strohm, Henning Ubbens, Dominika Mihalikova, Alexander Czarnowski, Paul Stamm, Michael Molitor, Stefanie Finger, Matthias Oelze, Dorothee Atzler, Philip Wenzel, Philipp Lurz, Thomas Münzel, Christian Weber, Esther Lutgens, Andreas Daiber, Steffen Daub

“…The present study investigates whether the CD40L-CD40-TRAF6 signaling pathway with focus on immune cells and adipocytes could be a therapeutic target in arterial hypertension.Arterial hypertension was induced in WT (C57BL6/J) and cell-specific CD40(L) knockout mice (AdipoqCre x CD40 fl/fl, CD4Cre x CD40 fl/fl, CD19Cre x CD40 fl/fl, and GP1baCre x CD40L fl/fl) via angiotensin (AT-II) infusion (1 mg/kg/d) for seven days. …”
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Maternal exposure to polystyrene nanoplastics during gestation and lactation caused fertility decline in female mouse offspring by Xiu Cheng, Yue Xue, Houpeng Wang, Zhangqiang Ma, Na Hu, Chenchen Zhang, Yu Gao, Ruihong Fan, Liaoliao Hu, Jia Li, Dalei Zhang, Jian Huang, Sitian Fang, Runting Xiao, Yuanqiao He, Tao Luo, Liping Zheng

“…This premature activation is likely due to the PS-NPs'induction of the AKT-FOXO3a signaling pathway by downregulating AMPK phosphorylation level and enhancing mTOR activity. …”
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Melatonin alleviates high temperature exposure induced fetal growth restriction via the gut-placenta-fetus axis in pregnant mice by Jia-Jin Wu, Xiaoyu Zheng, Caichi Wu, Wen Ma, Yibo Wang, Jun Wang, Yulong Wei, Xiangfang Zeng, Shihai Zhang, Wutai Guan, Fang Chen

“…In LPS-challenged pregnant mice, melatonin administration mitigated placental barrier injury and abnormal angiogenesis via the inactivation of the TLR4/MAPK/VEGF signaling pathway, ultimately leading to enhanced nutrient transportation in the placenta and thereby improving the fetal weight. …”
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Inducible Fgf13 ablation alleviates cardiac fibrosis via regulation of microtubule stability by Wang Cong, Wang Xiangchong, Zhang Yiyi, Mi Yuan, Han Yanxue, Zhi Yaxin, Zhao Ran, Cui Nanqi, Ma Qianli, Zhang Huaxing, Xue Dazhong, Qiao Ruoyang, Han Jiabing, Yu Yulou, Li Jiaxuan, Shaiea Mohammed, Liu Demin, Gu Guoqiang, Wang Chuan

“…Collectively, cardiac Fgf13 knockdown protects the heart from fibrosis in response to haemodynamic stress by modulating microtubule stabilization and ROCK signaling pathway.…”
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Effect of Xingbi Gel Nasal Drops on Rats with Allergic Rhinitis Based on the Nasal Mucosa-Hippocampus Neuroimmune Mechanism by FAN Xueqi, QIU Caixia, YAN Shuiping, ZHUANG Xiangli, AI Si, GUO Lihua, ZHENG Jian

“…Compared with the model group, mRNA transcription levels of Fyn, SP and VIP and average OD values of Fyn, SP and VIP in the hippocampus decreased significantly in the traditional Chinese medicine group and the western medicine group after treatment (<italic>P</italic>&lt;0.05), while mRNA transcription levels of NPY and average OD values of NPY increased significantly (<italic>P</italic>&lt;0.05).ConclusionXingbi gel nasal drops can improve the behavioral scores of rhinitis and learning and memory functions of rats with AR, which may be related to the regulation of nasal mucosa-hippocampus neuroimmune mechanism through the Fyn signaling pathway.…”
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ENO1 promotes PDAC progression by inhibiting CD8+ T cell infiltration through upregulating PD-L1 expression via HIF-1α signaling by Aziguli Tulamaiti, Shu-Yu Xiao, Yan Yang, Musitaba Mutailifu, Xia-Qing Li, Shi-Qi Yin, Hong-Tai Ma, Hong-Fei Yao, Lin-Li Yao, Li-Peng Hu, Jun Li, Shu-Heng Jiang, Zhi-Gang Zhang, Yan-Miao Huo, Dong-Xue Li, Xue-Li Zhang

“…Mechanistic studies revealed that ENO1 upregulated PD-L1 to prevent CD8+ T cells infiltration through the hypoxia-inducible factor (HIF)-1α signaling pathway, leading to PDAC progression.In conclusion, our findings indicate that ENO1 might serve as a potential biomarker for PDAC and a novel onco-immunotherapeutic target via its role in altering the TIME.…”
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VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment by Cheng Cheng, Qingrui Zha, Linmao Sun, Tianming Cui, Xinyu Guo, Changjian Xing, Zhengxiang Chen, Changyong Ji, Shuhang Liang, Shengwei Tao, Junhui Chu, Chenghui Wu, Qi Chu, Xuetian Gu, Ning Zhang, Yumin Fu, Shumin Deng, Yitong Zhu, Jiabei Wang, Yao Liu, Lianxin Liu

“…The accumulated G3P diffuses into the TME and directly interacts with SRC-family tyrosine kinase LCK, a critical component of the T-cell receptor (TCR) signaling pathway in CD8+T cells. This interaction heightens the phosphorylation of Tyr505, a key inhibitory residue, ultimately reducing LCK activity and impairing downstream TCR signaling. …”
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T Cell-Derived Apoptotic Extracellular Vesicles Ameliorate Bone Loss via CD39 and CD73-Mediated ATP Hydrolysis by Yang X, Zhou Y, Zhou F, Bao L, Wang Z, Li Z, Ding F, Kuang H, Liu H, Tan S, Qiu X, Jing H, Liu S, Ma D

“…The adenosine generated by ApoEVs inhibits the inflammatory response and promotes osteogenesis through A2BR and downstream PKA signaling.Conclusion: T cell-derived ApoEVs are enriched with CD39 and CD73, enabling them to hydrolyze extracellular ATP to adenosine, thereby promoting bone regeneration via A2BR and PKA signaling pathway. Our data underscore the substantive role of T cell-derived ApoEVs to treat osteoporosis, thus providing new ideas for the development of ApoEVs-based therapies in tissue regeneration. …”
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IFNβ drives ferroptosis through elevating TRIM22 and promotes the cytotoxicity of RSL3 by Huiyue Dong, Huiyue Dong, Ling Zhu, Ling Zhu, Jingjing Sun, Jingjing Sun, Qiuyan Chen, Qiuyan Chen, Pengyang Liu, Pengyang Liu, Wei Zhang, Huajing Zeng, Rong Lin, Rong Lin, Zongyang Yu, Zongyang Yu, Jun Lu, Jun Lu, Jun Lu, Jun Lu

“…STAT1, STAT3, TRIM21, and TRIM22 were silenced by siRNAs to examine their effects on IFNβ-induced ferroptosis. The cGAS-STING signaling pathway-activated mice were used to evaluate the effects of IFNβ on ferroptosis in vivo. …”
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Non-lethal sonodynamic therapy mitigates hypertensive renal fibrosis through the PI3K/AKT/mTORC1-autophagy pathway by DanDan Liu, Hui Wang, Jialong Li, Siqi Sheng, Shu Wang, Ye Tian

“…NL-SDT inhibited the transition of epithelial cells into myofibroblasts by activating PI3K-AKT-mTORC1-autophagy pathway in TGF-β1-induced NRK-52E cells. (3) The administration of the pathway inhibitors showed a reciprocal effect on NL-SDT-inhibited epithelial-mesenchymal transition (EMT). (1) NL-SDT reduced blood pressure temporarily in mice model of hypertensive renal fibrosis induced by Ang II. (2) NL-SDT alleviated renal fibrosis in mice model of hypertensive renal fibrosis induced by Ang II. (3) NL-SDT promoted autophagy by inhibiting PI3K-AKT-mTORC1 signaling pathway and alleviated renal fibrosis in mice model of hypertensive renal fibrosis induced by Ang II. …”
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CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer by Lorenzo Castagnoli, Alma Franceschini, Valeria Cancila, Matteo Dugo, Martina Bigliardi, Claudia Chiodoni, Paolo Toneguzzo, Viola Regondi, Paola A. Corsetto, Filippo Pietrantonio, Serena Mazzucchelli, Fabio Corsi, Antonio Belfiore, Antonio Vingiani, Giancarlo Pruneri, Francesca Ligorio, Mario P. Colombo, Elda Tagliabue, Claudio Tripodo, Claudio Vernieri, Tiziana Triulzi, Serenella M. Pupa

“…We provided evidence of a consistent enrichment of CD36 in HER2 + epithelial-mesenchymal transition (EMT)-like CSCs from all tested resistant cell models that mechanistically occurs via Wnt signaling pathway activation. Consistently, both in vitro and in vivo dual blockade of CD36 and HER2 increased the anti-CSC efficacy of anti-HER2 drugs favoring the transition of the therapy resistant mesenchymal CSCs into therapy-sensitive mesenchymal-epithelial transition (MET)-like epithelial state. …”
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Cardiac secreted HSP90α exacerbates pressure overload myocardial hypertrophy and heart failure by Le Pan, Chenxing Huang, Xuejuan Jin, Jian Wu, Kejia Jin, Jingyi Lin, Ying Wang, Jianxuan Li, Chao Yin, Xiang Wang, Lei Zhang, Guoping Zhang, Hangming Dong, Junli Guo, Issei Komuro, Yuxiang Dai, Yunzeng Zou, Hui Gong

“…These insights suggest the therapeutic potential of targeting HSP90α-initiated signaling pathway against cardiac hypertrophy and heart failure under pressure overload.…”
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