DAMPs prognostic signature predicts tumor immunotherapy, and identifies immunosuppressive mechanism of pannexin 1 channels in pancreatic ductal adenocarcinoma by Qianxue Wu, Qian Xiao, Qian Xiao, Xin Tang, Liuying Li, Daqiang Song, Yang Zhou, Benhua Li, Benhua Li, Guosheng Ren, Guosheng Ren, Fang Luo

“…Mechanically, through Bulk-RNA sequencing and experiments, we found that PANX1 promoted tumor progression and immune regulation via the ATP release to active NOD1/NFκB signaling pathway in PDAC.ConclusionOur in silico analyses established a classification system based on ICD-related DAMPs genes in PDAC, and constructed a DAMPs-related prognostic model to predict the efficacy of immunotherapy. …”
Get full text

Inhibition of the mTOR Pathway Exerts Cardioprotective Effects Partly through Autophagy in CLP Rats by Wen Han, Hao Wang, Longxiang Su, Yun Long, Na Cui, Dawei Liu

“…Moreover, the mTOR signaling pathway and LC3II, p62, and HIF-1a expression were measured by western blotting. …”
Get full text

Long-term neuropsychologic outcome of pre-emptive mTOR inhibitor treatment in children with tuberous sclerosis complex (TSC) under 4 months of age (PROTECT), a two-arm, randomized,... by Jan H. Driedger, Julian Schröter, PROTECT-Study Group, Steffen Syrbe, Afshin Saffari

“…On the molecular level, TSC is caused by heterozygous loss-of-function variants in either of the genes TSC1 or TSC2, encoding the Tuberin-Hamartin complex, which acts as a critical upstream suppressor of the mammalian target of rapamycin (mTOR), a key signaling pathway controlling cellular growth and metabolism. …”
Get full text

Therapeutic efficacy and mechanism of artesunate for mouse model of polycystic ovary syndrome by WANG Xueling, WANG Xueling, ZHONG Peiling, ZHONG Peiling, ZHAO Zhipeng

“…After steroidogenic human granulosa-like tumor cell line KGN were exposed to 100 μmol/L DHEA to simulate the hyperandrogen environment of PCOS, and then treated with 5 and 10 μg/mL AS for 24 h, the protein levels of endoplasmic reticulum stress signaling pathway was detected by Western blotting. Results‍　‍Compared with the Control group, the PCOS mice had disturbed estrous cycle, polycystic changes in the ovaries, and significantly increased serum T level and LH/FSH ratio (P<0.05), and obviously elevated HOMA-IR, TC and TG levels in terms of metabolism (P<0.01). …”
Get full text

Proteomic Analysis of MCF-7 Breast Cancer Cell Line Exposed To Leptin by A. Valle, J. Sastre-Serra, C. Pol, A. M. Miró, J. Oliver, P. Roca

“…Proteins regulated by leptin included proteins previously implicated in breast cancer such as catechol-o-methyltransferase, cathepsin D, hsp27, serine/threonine-protein phosphatase and regulatory proteins of the Ras signaling pathway. Proteins involved in other cellular functions such as stress response, cytosqueleton remodeling and proteins belonging to ubiquitin-proteasome system, were also identified. …”
Get full text

Identification and validation of immune and diagnostic biomarkers for interstitial cystitis/painful bladder syndrome by integrating bioinformatics and machine-learning by Tao Zhou, Can Zhu, Wei Zhang, Qiongfang Wu, Mingqiang Deng, Zhiwei Jiang, Longfei Peng, Hao Geng, Zhouting Tuo, Zhouting Tuo, Ci Zou

“…Moreover, PLAC8 overexpression was found to promote the proliferation of urothelial cells without affecting their migratory ability by inhibiting the Akt/mTOR/PI3K signaling pathway.ConclusionsOur study identifies potential diagnostic candidate genes and reveals the complex immune landscape associated with IC/BPS. …”
Get full text

Two-dimensional cell membrane chromatography guided screening of myocardial protective compounds from Yindan Xinnaotong soft capsule by Si-Min Shao, Xuan Ji, Xing Wang, Run-Zhou Liu, Yu-Ru Cai, Xiaobing Lin, Ze-Jie Zeng, Ling Chen, Liu Yang, Hua Yang, Wen Gao

“…Bioinformatic analysis utilizing RNA-seq indicated that the FOXO signaling pathway, with FOXO3 identified as a key target, plays a significant role in the cardioprotective effects of YD. …”
Get full text

Adaptive mechanisms to hypoxia and hyperoxia in juvenile turbot, Scophthalmus maximus by Yi Chen, Yuntian Zhang, Rongwei Zhang, Hongsheng Deng, Xiangyu Meng, Kotoya Inaba, Tatsu Osato, Xiaoran Zhao, Xiaoran Zhao, Yuzhe Han, Yuzhe Han, Tongjun Ren, Tongjun Ren

“…Transcriptomic analysis revealed various pathways implicated in adaptation to both hypoxic and hyperoxic conditions, encompassing amino acid metabolism, protein metabolism, lipid metabolism, carbohydrate metabolism, the PPAR signaling pathway, etc. However, pathway changes are not completely consistent. …”
Get full text

Ginseng-derived Nanoparticles Inhibit Lung Cancer Cell Growth by Promoting Macrophage M1 Polarization by Liangliang FAN, Limei REN, Song YANG, Wenjing LI, Yueming ZHAO, Ronghua ZHAO, Daqing ZHAO, Jiawen WANG

“…Conclusion: GDNPs induce apoptosis in lung cancer cells by stimulating M1 polarization of macrophages, inhibiting the proliferation of A549 cells, regulating the A549 cell cycle, and activating the TLR4/MyD88/NF-κB signaling pathway to promote the expression levels of inflammatory factors.…”
Get full text

IL-1 Receptor Antagonist Protects the Osteogenesis Capability of Gingival-Derived Stem/Progenitor Cells under Inflammatory Microenvironment Induced by Porphyromonas gingivalis Lipo... by Yuxin Zhao, Bobo Cai, Weijun Zhu, Jue Shi, Yu Wang, Misi Si

“…This might be explained by IL-1ra downregulating TLR4-mediated NF-κB signaling pathway activation.…”
Get full text

The influence of hepatocyte growth factor on the proliferation and differentiation of stem cells by Rafał Bieś, Agnieszka Mikosińska, Jakub Parys, Mateusz Litwin, Patrycja Kałuziak, Marta Jajczak, Maciej Mossakowski, Aleksandra Witek, Martyna Kaźmierczak, Stanisław Jesionek

“… Background: The HGF/c-Met signaling pathway is central to various biological processes, including tissue regeneration, cell proliferation, and differentiation. …”
Get full text

Fufang E’jiao Jiang’s effect on immunity, hematopoiesis, and angiogenesis via a systematic “compound-effect-target” analysis by Xiang Li, Xiao Xu, Ying Dong, Shusheng Fan, Xueyang Ren, Yuan Zheng, Jiamu Ma, Feng Zhang, Qingyue Deng, Xianxian Li, Yingyu He, Mengyu Sun, Wei Liu, Mengxia Li, Qing Xia, Yan Zhang, Gaimei She

“…Transcriptome data showed that several pathways such as complement and coagulation cascades, ECM-receptor interaction, and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ. 19 common targets were obtained through combined analysis of network pharmacology and transcriptomics, and 5 targets of them were verified by PCR. …”
Get full text

Osimertinib exacerbates immune checkpoint inhibitor-related severe adverse events by activating the IL-6/JAK/STAT3 pathway in macrophages by Yuan Li, Yanping Chen, Yuan Meng, Meng Shen, Fan Yang, Xiubao Ren

“…Conclusions: An osimertinib and ICI-induced irAE mouse model was constructed that showed osimertinib combined with ICIs inhibited EGFR phosphorylation and activated the IL-6/JAK/STAT3 signaling pathway in mouse liver macrophages, which led to the release of relevant cytokines.…”
Get full text

Integrated serum metabolomics and network pharmacology reveal molecular mechanism of Qixue Huazheng formula on peritoneal fibrosis by Xiaohui Meng, Xiaohui Meng, Li Sheng, Yongqing You, Yongqing You, Huibo Dai, Manshu Yu, Funing Wang, Funing Wang, Ziren Zhou, Ziren Zhou, Yun Shan, Meixiao Sheng

“…Integrated data analysis indicated that steroid hormone biosynthesis, the Ras signaling pathway, apoptosis, and estrogen signaling contributed to the effects of QXHZF. …”
Get full text

Effect of Matrine on growth performance, gut health, and gut microbiota in chickens infected with avian pathogenic Escherichia coli by Ningning Mao, Yaming Yu, Jiqin Cui, Jin He, Yang Yang, Deyun Wang

“…In summary, matrine alleviated APEC-induced intestinal inflammation and damage, potentially by inhibiting NF-κB signaling pathway and reshaping the gut microbiota. These findings provide promising insights into the prevention and treatment of avian colibacillosis.…”
Get full text

Integrating rapamycin with novel PI3K/Akt/mTOR inhibitor microRNAs on NOTCH1-driven T-cell acute lymphoblastic leukemia (T-ALL) by Fateme Arjmand, Samaneh Shojaei, Mitra Khalili, Hossein Dinmohammadi, Behzad Poopak, Samira Mohammadi-Yeganeh, Yousef Mortazavi

“…Introduction: The PI3K/AKT/mTOR signaling pathway plays a significant role in the development of T-cell acute lymphoblastic leukemia (T-ALL). …”
Get full text

Bleomycin pollution and lung health: The therapeutic potential of peimine in bleomycin-induced pulmonary fibrosis by inhibiting glycolysis by Kangchen Li, Xuefang Liu, Ruilong Lu, Peng Zhao, Yange Tian, Jiansheng Li

“…Our findings also indicate that peimine, a natural compound derived from Fritillaria, suppresses fibroblast activation and ameliorates pulmonary fibrosis by inhibiting glycolysis through the PI3K/Akt/PFKFB3 signaling pathway. Taken together, this study underscores the environmental and health risks associated with the accumulation of cytostatic drugs like BLM and highlights the therapeutic potential of natural compounds such as peimine. …”
Get full text

The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics by Jun Wang, Xiuxiu Zhang, Zhijing Ni, Elnur Elam, Kiran Thakur, Kexin Li, Chuyan Wang, Jianguo Zhang, Zhaojun Wei

“…A sum of 431 targets, the overlap of predicted mRNAs from TargetScan, miRDB, and miRtarbase were detected as the targets for these dysregulated miRNAs. FoxO signaling pathway was the hub pathway for the targets. …”
Get full text

Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…RNA sequencing of OGD in pericytes was performed to identify a key signaling pathway. Results We observed that both medium and high concentrations of EDB could ameliorate CCH-induced cognitive impairment and emotional disorders. …”
Get full text

Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regenera... by Zhaoyang Gong, Zhenhao Chen, Dachuan Li, Xiao Lu, Jianwei Wu, Hanqiu Sun, Ximeng Wang, Siyang Liu, Xinlei Xia, Feizhou Lu, Jianyuan Jiang, Chi Sun, Hongli Wang, Feng Zeng, Xiaosheng Ma

“…Notably, the composite significantly upregulated the expression of nerve growth-associated proteins, reduced inflammatory cytokines, scavenged reactive oxygen species (ROS), and promoted M2 macrophage polarization by inhibiting the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway. In a rat SCI model, it facilitated functional recovery and attenuated inflammation. …”
Get full text