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1921
Appearance of L90I and N205S Mutations in Effector Domain of NS1 Gene of pdm (09) H1N1 Virus from India during 2009–2013
Published 2014-01-01“…In the present study, full length sequencing of NS gene was done in 91 samples which were obtained from patients over the time period of five years from 2009 to 2013. …”
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1922
Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran
Published 2014-01-01“…OXA-48, NDM-1, and CTX-M-15 genes were detected by PCR and sequencing methods. The outer membrane porins such as OmpK35 and OmpK36 were analysed by SDS-PAGE, PCR, and sequencing methods. …”
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1923
Case study of a neuroendocrine tumor of uncertain origin: single-cell transcriptomics unravels potential primary location
Published 2024-12-01“…The tumor was subjected to single-cell RNA sequencing (scRNA-seq) and whole exome sequencing (WES). …”
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1924
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology /
Published 2018Table of Contents: “…Biochemical and molecular biological methods in life sciences studies -- Basic principles -- Cell culture techniques -- Recombinant DNA techniques and molecular cloning -- Preparative protein biochemistry -- Electrophoretic techniques -- Immunochemical techniques -- Flow cytometry -- Radioisotope techniques -- Principles of clinical biochemistry -- Microscopy -- Centrifugation and ultracentrifugation -- Spectroscopic techniques -- Basic techniques probing molecular structure and interactions -- Mass spectrometric techniques -- Fundamentals of bioinformatics -- Fundamentals of chemoinformatics -- The python programming language -- Data analysis -- Fundamentals of genome sequencing and annotation -- Fundamentals of proteomics -- Fundamentals of metabolomics -- Enzymes and receptors -- Drug discovery and development.…”
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1925
Galactosaminoglycan Function and Oligosaccharide Structure Determination
Published 2007-01-01“…This review will discuss the importance of sequencing long chondroitin sulfate and dermatan sulfate chains specifically derived from decorin. …”
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1926
Cancer, Senescence, and Aging: Translation from Basic Research to Clinics
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1927
Characterization of avian adenovirus FAdV-4/6/2009 isolate RABIES
Published 2018-04-01“…The virus was isolated in chicken Leghorn male hepatoma cell culture that exhibited cytopathogenic effect and identified as avian type С adenovirus by PCR followed by sequencing of hexon gene fragment. Hydropericardium syndrome developed in 7-day-old broiler chickens experimentally orally infected with the virus.…”
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1928
A Case Report of Chlamydia psittaci Infective Endocarditis Complicated With Pneumonia
Published 2025-01-01“…A rare case of infective endocarditis complicated by pneumonia caused by Chlamydia psittaci was reported, which was diagnosed using metagenomic next-generation sequencing (mNGS). The patient recovered after receiving appropriate anti-infective treatment. …”
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1929
T-PLL Presenting with an Indolent Course
Published 2024-01-01“…T-cell receptor clonality assessment by next-generation sequencing revealed a dominant clone in the ß-chain constant region. …”
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1930
Characterization of CO1 and 18S rDNA genes from Indonesian native ornamental shrimp Caridina boehmei cultured in Jakarta
Published 2024-07-01“…The CO1 sequencing produced fragments consisting of 25.84% adenine, 20.95% cytosine, 19.11% guanine, and 34.10% thymine. …”
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1931
Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies
Published 2025-02-01“…DD-ITD implementation would entail building proficiency in advanced molecular laboratory techniques and data analysis, and increased demand for confirmatory sequencing.IMPORTANCESurveillance of acute flaccid paralysis (AFP) and sensitive poliovirus detection are key components of the WHO Global Polio Eradication Strategy. …”
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1932
Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease
Published 2025-01-01“…Single-nucleus RNA sequencing (snRNA-seq) technology is a high-throughput sequencing analysis technique for RNA at the level of a single cell nucleus. …”
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1933
Congenital muscular dystrophies and myopathies: the leading cause of genetic muscular disorders in eleven Chinese families
Published 2025-01-01“…Nanopore sequencing and optical genome mapping were applied for detecting complicated genome rearrangements. …”
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1934
Atezolizumab plus bevacizumab in patients with unresectable or metastatic mucosal melanoma: 3‐year survival update and multi‐omics analysis
Published 2025-01-01“…Available samples underwent whole exome sequencing, transcriptome sequencing and targeted bisulphite sequencing to assess correlations with clinical outcomes. …”
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1935
Ophiostomatoid fungi associated with pine bark beetles and infested pines in south-eastern Australia, including Graphilbum ipis-grandicollis sp. nov.
Published 2021-09-01“…This study also provides an early example of whole genome sequencing (WGS) approaches replacing traditional PCR-based methods for taxonomic surveys. …”
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1936
No microorganism was detected in amniotic fluid of healthy pregnancies from the second trimester to the delivery
Published 2025-01-01“…While numerous studies have attempted to determine the presence or absence of intrauterine bacteria, the majority of them have drawn conclusions based on sequencing data from maternal or infant samples taken at a single time point. …”
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1937
Pathogenicity of avian reovirus variant in the immune organs of broiler chicks
Published 2025-03-01“…In this study, an ARV strain (Chicken/XJ/China/2022/01/16) was isolated from arthritic broiler chicks in Xinjiang province and whole genome sequencing of the ARV strain was performed using next generation sequencing (NGS) technology, and sequence analysis showed that the strain was a type V variant of the ARV gene. …”
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1938
The Development of a One-Step PCR Assay for Rapid Detection of an Attenuated Vaccine Strain of Duck Hepatitis Virus Type 3 in Korea
Published 2024-12-01“…The clinical sample results were completely consistent with those obtained using nested PCR detection and gold-standard sequencing. In summary, we successfully developed a rapid, one-step MAMA-PCR method that is more suitable for clinical diagnosis than traditional sequencing methods.…”
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1939
A Highly Efficient System for Separating Glandular and Non-glandular Trichome of Cucumber Fruit for Transcriptomic and Metabolomic Analysis
Published 2025-01-01“…However, current methods for separating trichomes encounter challenges such as low efficiency and insufficient accuracy, limiting their applicability in multi-omics sequencing studies. This protocol introduces an efficient system designed for the precise separation of glandular and non-glandular trichomes from cucumber fruit. …”
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1940
Rare coinheritance of hemoglobin vancleave with severe beta-thalassemia mutation in a patient with secondary erythrocytosis
Published 2024-04-01“…The diagnosis of Hb Vancleave, in combination with codon 41/42 (-TTCT) (NM_000518.5:c.126_129del; dbSNP: rs80356821), was confirmed by direct sequencing. This report highlights the importance of sequencing in the differential diagnosis of beta-thalassemia syndrome in Malaysia.…”
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