Molecular Identification of Fusarium proliferatum from toenails: A case report by Sazan Jamal Gharib, Nawroz Abdul-razzak Tahir

“…For final identification sequencing of DNA has been carried out by [Macrogen Korea] using forward primer ITS1, The obtained consensus of query sequence was compared with the ITS DNA database on the BLAST homepage, aligned and diagnosed strain deposited to the GenBank and we received an accession number (MK112619). …”
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Altered Protein Structures and Neoepitopes in Lupus Neutrophils From Dysregulated Splicing of Messenger RNA by Rayan Najjar, Xiaoxing Wang, Jose Mario Bello Pineda, Hugh Alessi, Alison Bays, Robert K. Bradley, James N. Jarvis, Tomas Mustelin

“…RNA sequencing from SARS‐CoV2–infected individuals was used as an additional comparator. …”
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Technical considerations towards commercialization of porcine respiratory and reproductive syndrome (PRRS) virus resistant pigs by A. Mark Cigan, Pieter W. Knap

“…As traditional methods of classical breeding and selection for trait improvement are slow, recent progress in several areas of biology including (a) understanding host–pathogen interactions, (b) inexpensive and rapid DNA sequencing, and (c) robust gene editing like CRISPR-Cas provide geneticists tools to accelerate discovery and deployment of disease resistance alleles in livestock. …”
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Tumor mutational burden quantification from targeted gene panels: major advancements and challenges by Sara Gandini, Laura Fancello, Pier Giuseppe Pelicci, Luca Mazzarella

“…TMB can be quantitated by a number of NGS-based sequencing technologies. Whole Exome Sequencing (WES) allows comprehensive measurement of TMB and is considered the gold standard. …”
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SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice by Chunxia Yu, Peng Zhang, Sujuan Liu, Yanmei Niu, Li Fu

“…Fecal microbiota composition and function were assessed by 16S rRNA sequencing. The sequencing results showed that SESN2−/− mice displayed differed microbiome profile from WT mice. …”
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Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia by Xiuchun Yang, Yijun Zong, Zhentian Zhang, Yan Zhao, Xueying Gao, Jie Zhang, Qian Hou, Renyi Li, Bing Xiao

“…Peripheral venous blood samples from patients with CAE were collected for RNA sequencing to identify differentially expressed genes (DEGs), followed by functional enrichment. …”
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Heme Oxygenase 1/Peroxisome Proliferator-Activated Receptor Gamma Pathway Protects Intimal Hyperplasia and Mitigates Arteriovenous Fistula Dysfunction by Regulating Oxidative Stres... by Tingfei Xie, Yunpeng Xu, Lecai Ji, Xiaolu Sui, Aisha Zhang, Yanzi Zhang, Jihong Chen

“…AVF model mice were established, and the vascular tissues from the arteriovenous anastomosis site were sent for mRNA sequencing. Differentially expressed mRNAs (DEmRNAs) were screened and subjected to functional analysis. …”
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Exploring RNA modifications in infectious non-coding circular RNAs by Pavel Vopalensky, Anton Škríba, Michela Chiumenti, Lucia Ďuričeková, Anna Šimonová, Ondřej Lukšan, Francesco di Serio, Beatriz Navarro, Hana Cahova

“…ONT sequencing predicted three m6A positions. Employing orthogonal SELECT method, we confirmed m6A in two positions A353 and A360, which are highly conserved among CEVd variants. …”
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Single-cell landscape of dynamic changes in CD8+ T cells, CD4+ T cells and exhausted T cells in hepatocellular carcinoma by Rongqiang Liu, Jing Ye, Jianguo Wang, Wangbin Ma, Zhendong Qiu, Jia Yu, Weixing Wang

“…In this study, we investigated the value of T-cell-related genes in prognosis by single-cell sequencing data in hepatocellular carcinoma. Twelve cases of hepatocellular carcinoma single-cell sequencing were included in the study. …”
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Identifying novel heterozygous PI4KA variants in fetal abnormalities by Chen Cheng, Fan Yang, Xinlin Chen, Sheng Zhao

“…Novel PI4KA variants were detected through clinical Whole exon sequencing (WES) and validated by Sanger sequencing. …”
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The technology landscape for detection of DNA methylation in cancer liquid biopsies by Isabelle Neefs, Joe Ibrahim, Marc Peeters, Guy Van Camp, Ken Op de Beeck

“…Furthermore, we provide some insights into how the field might evolve in the future, where we believe enzymatic conversion might become a new gold standard and direct sequencing methods, such as ONT-sequencing, will get an important place in the epigenetic research field. …”
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Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus by Li Zou, Shirong Yan, Xueping Guan, Yunjun Pan, Xin Qu

“…Next, methylated DNA immunoprecipitation chip (MeDIP-chip) was used for detection. Bisulfite sequencing PCR (BSP) and gene sequencing were then performed to detect and analyze the correlation between PRKCZ gene promoter methylation and T2DM. …”
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Single-cell transcriptomic profiling of heart reveals ANGPTL4 linking fibroblasts and angiogenesis in heart failure with preserved ejection fraction by Guoxing Li, Huilin Zhao, Zhe Cheng, Junjin Liu, Gang Li, Yongzheng Guo

“…Cells from the hearts were subjected to single-cell sequencing. The key protein expression was measured with Immunohistochemistry and immunofluorescence staining. …”
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A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System by Sangdun Choi, Mi Sook Chang, Tara Stuecker, Christine Chung, David A. Newcombe, Kasthuri Venkateswaran

“…Several hundred clones were randomly selected for sequencing, followed by Blastn/Blastx searches. Sequences encoding specific genes from Burkholderia, a species abundant in the soil and groundwater, were found in both samples. …”
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Retinitis Pigmentosa with EYS Mutations Is the Most Prevalent Inherited Retinal Dystrophy in Japanese Populations by Yuuki Arai, Akiko Maeda, Yasuhiko Hirami, Chie Ishigami, Shinji Kosugi, Michiko Mandai, Yasuo Kurimoto, Masayo Takahashi

“…This study found 43 novel sequence variants. In silico analysis suggests that 20 of 25 novel missense variants are pathogenic. …”
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Microbial bioindicators associated with cadmium uptake in sixteen genotypes of Theobroma cacao by Jennifer E. Schmidt, Caleb A. Lewis, Alana J. Firl, Pathmanathan Umaharan

“…Sequencing highlighted over 200 amplicon sequence variants (ASVs) whose relative abundance was related to cacao leaf and stem Cd content or concentration. …”
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Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa by Lungile N. Mlangeni, Tsepo Ramatla, Kgaugelo E. Lekota, Cormac Price, Oriel Thekisoe, Che Weldon

“…The isolates were identified using polymerase chain reaction (PCR) by amplifying the invA gene and were further confirmed by 16S rRNA gene sequencing. Salmonella serovars were detected in chameleons (36.8%), lizards (31.6%), snakes (15.8%), and tortoises (15.8%). …”
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Conducting genetic epidemiology research on hyperuricaemia and gout in an indigenous community in Guam - a feasibility study by Yvette C. Paulino, Frank Camacho, Tristan VC Paulino, Delores J. Lee, Lisa Linda Natividad, Elizabeth Matisoo-Smith, Tony R. Merriman, Anna L. Gosling

“…The results of ongoing genetic sequencing will be used to understand molecular causes of gout in Guam.…”
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Prevalence and methodologies for detection, characterization and subtyping of Listeria monocytogenes and L. ivanovii in foods and environmental sources by Jin-Qiang Chen, Patrick Regan, Pongpan Laksanalamai, Stephanie Healey, Zonglin Hu

“…In this review, we (A) described L. monocytogenes and L. ivanovii, world-wide incidence of listeriosis, and prevalence of various L. monocytogenes strains in food and environmental sources; (B) comprehensively reviewed different types of traditional and newly developed methodologies, including culture-based, antigen/antibody-based, LOOP-mediated isothermal amplification, matrix-assisted laser desorption ionization-time of flight-mass spectrometry, DNA microarray, and genomic sequencing for detection and characterization of L. monocytogenes in foods and environmental sources; (C) comprehensively summarized different subtyping methodologies, including pulsed-field gel electrophoresis, multi-locus sequence typing, ribotyping, and phage-typing, and whole genomic sequencing etc. for subtyping of L. monocytogenes strains from food and environmental sources; and (D) described the applications of these methodologies in detection and subtyping of L. monocytogenes in foods and food processing facilities.…”
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Genetic Variation and Population Structure in Jamunapari Goats Using Microsatellites, Mitochondrial DNA, and Milk Protein Genes by P. K. Rout, K. Thangraj, A. Mandal, R. Roy

“…We have analysed genetic variation and population history in Jamunapari goats based on 17 microsatellite loci, 2 milk protein loci, mitochondrial hypervariable region I (HVRI) sequencing, and three Y-chromosomal gene sequencing. …”
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