Genome-wide identification of short-chain dehydrogenases/reductases genes and functional characterization of ApSDR53C2 in melanin biosynthesis in Arthrinium phaeospermum by Jiao Liao, Yisi Wang, Han Liu, Sijia Liu, Peng Yan, Hang Chen, Shujiang Li, Shujiang Li

“…Candidate ApSDRs screened by transcriptome sequencing were compared by qPCR experiments to obtain key ApSDRs that may play an important role in infestation and adversity resistance. …”
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Pseudodominant Nanophthalmos in a Roma Family Caused by a Novel PRSS56 Variant by Lubica Dudakova, Pavlina Skalicka, Olga Ulmanová, Martin Hlozanek, Viktor Stranecky, Frantisek Malinka, Andrea L. Vincent, Petra Liskova

“…DNA of subjects with nanophthalmos was analysed by exome sequencing. Sanger sequencing was applied for targeted screening of potentially pathogenic variants and to follow segregation of identified variants within the family. …”
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Biallelic mutations in CDC20 cause female infertility due to oocyte maturation abnormality by Guangzhong Jiao, Jinhao Xing, Zhaoli Du, Hongchu Bao, Xiaoyan Liu

“…Through rigorous validation using Sanger sequencing technology, both of her parents have been confirmed as genetic carriers of these specific mutations. …”
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Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus by J. Petersen, C.P. Colon, J.L. Joyner

“…Both crab gut and fecal flush samples did not sequence well, showing low species diversity and very high variability. …”
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Comparative analysis of microbial contamination in diesel fuels using MALDI-TOF MS by Agnieszka Ludwiczak, Tomasz Zieliński, Ewelina Sibińska, Grażyna Czeszewska-Rosiak, Michał Złoch, Joanna Rudnicka, Andrzej Tretyn, Paweł Pomastowski

“…Detection and characterization of microbiological contamination in fuels were performed with further validation by 16 S rRNA gene sequencing. Microorganisms collected from three types of diesel fuel were cultured, and fuel samples were analyzed. …”
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KYNU as a Biomarker of Tumor-Associated Macrophages and Correlates with Immunosuppressive Microenvironment and Poor Prognosis in Gastric Cancer by Kaiyu Shen, Binyu Chen, Liu Yang, Wencang Gao

“…GC-associated single-cell RNA sequencing and bulk RNA sequencing (bulk-seq) data were obtained from the Gene Expression Omnibus and The Cancer Genome Atlas databases, respectively. …”
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Tracking the genetic diversity of SARS-CoV-2 variants in Nicaragua throughout the COVID-19 pandemic by Gerald Vásquez Alemán, Cristhiam Cerpas, Jose G. Juarez, Hanny Moreira, Sonia Arguello, Josefina Coloma, Eva Harris, Aubree Gordon, Shannon N. Bennett, Ángel Balmaseda

“…This study characterizes the genomic diversity of SARS-CoV-2 in Nicaragua from March 2020 through December 2022, utilizing 1064 genomes obtained via next-generation sequencing. These sequences were selected nationwide and analyzed for variant classification, lineage predominance, and phylogenetic diversity. …”
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The first engkabang jantong (Rubroshorea macrophylla) genome survey dataNCBI GenBank by Hung Hui Chung, Asmeralda Ai Leen Soh, Melinda Mei Lin Lau, Han Ming Gan, Siong Fong Sim, Leonard Whye Kit Lim

“…Only 2.8 % and 1 % of them are fragmented and missing respectively. The short-read sequencing results obtained from the Illumina platform in this study will be essential to complement the Nanopore long-read sequencing results in hybrid genome assembly endeavors in the near future.…”
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Phylogeography of the woolly mammoth (<i>Mammuthus primigenius</i>) in the Minusinsk Depression of southern Siberia in the Late Pleistocene by S. A. Modina, M. A. Kusliy, D. G. Malikov, A. S. Molodtseva

“…Within the framework of this effort, libraries for high-throughput sequencing of seven bone samples of woolly mammoths were obtained, two-round enrichment using biotinylated probes of modern mtDNA of Elephas maximus immobilised on magnetic microspheres and sequencing with subsequent bioinformatic analysis were carried out. …”
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Characteristics of transcriptome and chromatin accessibility in the peripheral blood after acute hypoxia exposure by Kuo Zeng, Pei-ru Yuan, Jin-feng Xuan, Lai-xi Zhao, Xiao-na Li, Jun Yao, Dong Zhao

“…Results Hence, we performed RNA sequencing (RNA-seq) and ATAC sequencing (ATAC-seq) to observe the changes of transcriptome and chromatin accessibility in the peripheral blood of eight individuals at 1 h post adaptation in a simulated plateau environment with 3500 m and 4500 m altitude, respectively. …”
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Gut Microbiome Profiles in Colorectal Cancer Patients in Iraq by Fatima J. Kadhim, Zahid S. Aziz, Khalid S. Ibrahim

“…Bacterial DNA was extracted, followed by 16S rRNA amplicon sequencing to profile the microbial communities. The results indicated significant differences between the fecal microbiome of the two groups. …”
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A Chromosomal-Level Genome of Dermatophagoides farinae, a Common Allergenic Mite Species by Rongxuan Hu, Haifeng Huang, Ying Zhou, Yanshan Liu, Yaning Ren, Yuanfen Liao, Cunyin Yuan, Xiaohong Gu, Yubao Cui

“…We achieved a chromosome-level assembly of D. farinae’s genome by integrating PacBio single-molecule real-time sequencing, Illumina paired-end sequencing, and Hi-C technology, followed by annotating allergens and mapping them to specific chromosomes. …”
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DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status by Xiwen Dong, Fanqi Hu, Jing Yi, Yuning Zhang, Chao Liu, Panpan Geng, Han Duan, Chu-Tse Wu, Xuesong Zhang, Hua Wang

“…To understand the biological effects of dental pulp stem cells (DPSCs) on nucleus pulposus (NP) cells, we carried out RNA sequencing, bioinformatic analysis which unveiled gene expression differences, and pathway variation in primarily isolated patients’ NP cells after treatment with DPSCs supernatant. …”
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Intratumor heterogeneity: models of malignancy emergence and evolution by R. A. Ivanov, S. A. Lashin

“…Furthermore, single­cell sequencing methods have emerged as powerful tools for dissecting intratumoral heterogeneity and tracing clonal evolution. …”
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Identification of a Novel Non-V600E BRAF Mutation in Papillary Thyroid Cancer by Marco Capezzone, Maja Rossi, Elisabetta Macerola, Silvia Cantara, Francesco Pepe, Eugenia Maria Morabito, Gilda Dalmazio, Sara Bardi, Agostino Ognibene, Massimo Alessandri, Gabriele Materazzi, Luigi De Napoli, Michele Cirianni, Liborio Torregrossa

“…The presence of this new variant of BRAF mutation was subsequently confirmed in the postoperative phase by direct Sanger sequencing. In conclusion, we report a new non-V600E variant previously undetected in papillary thyroid cancer. …”
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NLRP3 Is Expressed in the Spiral Ganglion Neurons and Associated with Both Syndromic and Nonsyndromic Sensorineural Deafness by Penghui Chen, Longxia He, Xiuhong Pang, Xiaowen Wang, Tao Yang, Hao Wu

“…Though a variety of targeted next-generation sequencing (NGS) panels has been recently developed to facilitate genetic screening of nonsyndromic deafness, some syndromic deafness genes outside the panels may lead to clinical phenotypes similar to nonsyndromic deafness. …”
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Mitochondrial DNA Structure in <i>Trypanosoma cruzi</i> by Alfonso Herreros-Cabello, Francisco Callejas-Hernández, Manuel Fresno, Núria Gironès

“…However, next-generation sequencing (NGS) methods have resolved these issues, enabling the complete sequencing of maxicircles from different strains. …”
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The First Infant Bloodstream Infection Caused by Pantoea dispersa in China: A Case Report and Literature Review by Yu W, Sun Z, Wang M, Li Z, Zhang C, Sun Y, Wang S

“…Through MALDI Biotyper and next-generation sequencing techniques, the pathogen was identified as P. dispersa. …”
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Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host. by Martine Bangratz, Aurore Comte, Estelle Billard, Abdoul Kader Guigma, Guillaume Gandolfi, Abalo Itolou Kassankogno, Drissa Sérémé, Nils Poulicard, Charlotte Tollenaere

“…We aimed to decipher the distinct RYMV isolates that simultaneously infect a single rice plant and to sequence their genomes. To this end, we tested different sequencing strategies, and we finally combined direct cDNA ONT (Oxford Nanopore Technology) sequencing with the bioinformatics tool RVhaplo. …”
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STK3 Suppresses Ovarian Cancer Progression by Activating NF-κB Signaling to Recruit CD8+ T-Cells by Xiangyu Wang, Fengmian Wang, Zhi-Gang Zhang, Xiao-Mei Yang, Rong Zhang

“…RNA sequencing and gene set enrichment analysis (GSEA) were used to compare the transcriptome changes in the STK3 overexpression ovarian cancer and control cells. …”
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