Identification of the Hub Genes Involved in Stem Cell Treatment for Intervertebral Disc Degeneration: A Conjoint Analysis of Single-Cell and Machine Learning by Jianfeng Chen, Fuwei Qi, Guanshen Li, Qiaosong Deng, Chenlin Zhang, Xiaojun Li, Yafeng Zhang

“…In addition, the results of single-cell sequencing demonstrated the critical function of stem cells in IDD. …”
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Emerging technologies of single-cell multi-omics by Yi June Kim, Koichi Takahashi

“… The heterogeneity of the hematopoietic system was largely veiled by traditional bulk sequencing methods, which measure the averaged signals from mixed cellular populations. …”
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Isolation, Characterization, and Identification Candidate of Probiotic Bacteria Isolated from Wadi Papuyu (Anabas testudineus Bloch.) a Fermented Fish Product from Central Kalimant... by Yulistia Budianti Soemarie, Tiana Milanda, Melisa Intan Barliana

“…Identification results using the VITEK 2 Compact method and 16S rRNA gene PCR-sequencing showed that of the 4 isolated strains, three were bacterial and one belonged to yeasts.…”
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scRNA‐seq data analysis method to improve analysis performance by Junru Lu, Yuqi Sheng, Weiheng Qian, Min Pan, Xiangwei Zhao, Qinyu Ge

“…Abstract With the development of single‐cell RNA sequencing technology (scRNA‐seq), we have the ability to study biological questions at the level of the individual cell transcriptome. …”
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Dynamic Phase Signal Control Method for Unstable Asymmetric Traffic Flow at Intersections by Xiancai Jiang, Yu Jin, Yanli Ma

“…This paper addresses the limitations that the phases proposed in variable phase sequencing studies for stochastic traffic flow are all predetermined and that the variable phase sequencing is only suitable for low traffic volume environment. …”
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Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids by Lu Zhu, Wenhao Yang, Jiaxin Luo, Danli Lu, Yanan Hu, Rui Zhang, Yan Li, Li Qiu, Zelian Chen, Lina Chen, Hanmin Liu

“…RNA sequencing analysis of differentially expressed genes indicated a significant reduction in the immune response in NO. …”
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Preliminary exploration of mRNA, lncRNA, and miRNA expressions in the bovine jejunum unveils novel aspects of Mycobacterium avium subspecies paratuberculosis infections by Yahui Gao, Jie Cao, Bo Han, Dongxiao Sun

“…Results We applied whole transcriptome sequencing analysis to examine the jejunum tissue in nine Holstein cows. …”
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Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats by Tao-tao Lv, Yan-jun Mo, Tian-yuan Yu, Shuai Shao, Meng-qian Lu, Yu-ting Luo, Yi Shen, Yu-mo Zhang, Wong Steven

“…PCR results were consistent with those of RNA sequencing, suggesting that the results of transcriptome sequencing were reliable. …”
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Slight thermal stress exerts genetic diversity selection at coral (Acropora digitifera) larval stages by Cristiana Manullang, Nozomi Hanahara, Ariyo Imanuel Tarigan, Yuko Abe, Mao Furukawa, Masaya Morita

“…Many larval protein-coding sequences were identified in this genomic region, and the codon evolution of many of these genes showed signs of positive selection. …”
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Massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS) by Zhi Weng, Jiangxue Li, Yi Wu, Xuehao Xiu, Fei Wang, Xiaolei Zuo, Ping Song, Chunhai Fan

“…Nevertheless, unbiased information retrieval from low-copy-number sequences remains a barricade that largely arises from the indispensable DNA amplification. …”
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Exercise-induced methylation of the Serhl2 promoter and implication for lipid metabolism in rat skeletal muscle by Mutsumi Katayama, Kazuhiro Nomura, Jonathan M. Mudry, Alexander V. Chibalin, Anna Krook, Juleen R. Zierath

“…To determine the skeletal muscle DNA methylation signature of endurance swim training, we used whole-genome methylated DNA immunoprecipitation (MeDIP) sequencing. Methods: We utilized endurance-trained rats, cultured L6 myotubes, and human skeletal muscle cells, employing MeDIP sequencing, gene silencing, and palmitate oxidation assays. …”
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Identification of prognostic biomarkers of sepsis and construction of ceRNA regulatory networks by Guihong Chen, Wen Zhang, Chenglin Wang, Muhu Chen, Yingchun Hu, Zheng Wang

“…Subsequently, GO analysis was performed on the differential expression genes; Meta-analysis was used to screen for prognostic related genes; 10 × Single-cell RNA sequencing was used to annotate the cell distribution of core genes. …”
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Fully automated extraction of high-quality total nucleic acids from FFPE specimens for comprehensive genomic profiling of solid tumors by Kristopher Amirault, Michael Collins, Luca Beker, Brandon Mills, Martina Werner, Jonathan Andreas, Daniel Hartman, Jordan Dargert, Vanessa Process, Sean Cederlund, Thuy Dao, Linnea Menin, Molly Ferrara, Andrew Briggs, Joshua Shreve, Daniel Metzger, Angela Stout, Erin Deblasi, Jie An, Taylor Jensen, Shengle Zhang, Vinesh Rana, Bryn Burlingame, Ulrich Thomann, Durga Prasad Dash, Sha Liao, Jeffrey M. Conroy, Eugenio Daviso

“…By identifying actionable mutations through next-generation sequencing of solid tumors CGP enables targeted therapy decisions. …”
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Will the Amaranthus tuberculatus Resistance Mechanism to PPO-Inhibiting Herbicides Evolve in Other Amaranthus Species? by Chance W. Riggins, Patrick J. Tranel

“…However, sequencing revealed two distinct repeat patterns encompassing the mutation site. …”
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Novel Mutations in the MKKS, BBS7, and ALMS1 Genes in Iranian Children with Clinically Suspected Bardet–Biedl Syndrome by Roghayeh Dehghan, Mahdiyeh Behnam, Mansoor Salehi, Roya Kelishadi

“…In this study, we used whole-exome sequencing (WES) to investigate the underlying mutations in four Iranian children from consanguineous families with a clinical diagnosis of Bardet–Biedl syndrome (BBS). …”
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Characterization of the Emerging Enteropathogen Escherichia Albertii Isolated from Urine Samples of Patients Attending Sapporo Area Hospitals, Japan by Miyuki Fujioka, Sho Yoshioka, Masahiko Ito, Chowdhury Rafiqul Ahsan

“…In this study, we performed biochemical characterization, gene possession status, drug susceptibility testing, and sequencing analysis of the strains detected in urine samples. …”
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A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar 'Varnes'. by Daniel James Sargent, Matteo Buti, Stefan Martens, Claudio Pugliesi, Kjersti Aaby, Dag Røen, Chandra Bhan Yadav, Felicidad Fernández Fernández, Muath Alsheikh, Jahn Davik, R Jordan Price

“…By performing RNASeq, we revealed differential expression patterns in the apricot-fruited 'Varnes' for genes in the anthocyanin biosynthesis pathway and following whole genome sequencing using long-read Oxford Nanopore Technologies sequencing, we identified a CACTA-like transposable element (TE) in the second exon of the Anthocyanidin synthase (Ans) gene that caused a truncated predicted ANS protein. …”
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HaVec: An Efficient de Bruijn Graph Construction Algorithm for Genome Assembly by Md Mahfuzer Rahman, Ratul Sharker, Sajib Biswas, M. Sohel Rahman

“…The rapid advancement of sequencing technologies has made it possible to regularly produce millions of high-quality reads from the DNA samples in the sequencing laboratories. …”
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Prospects for marker-associated selection in tomato <i>Solanum lycopersicum</i> L. by A. B. Shcherban

“…A separate chapter is devoted to the use of various types of DNA markers for constructing detailed genetic maps of the specified object, which, along with full-genome sequencing data, can be used to screen for genes responsible for breeding traits. …”
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Advanced Genetic Testing Comes to the Pain Clinic to Make a Diagnosis of Paroxysmal Extreme Pain Disorder by Ashley Cannon, Svetlana Kurklinsky, Kimberly J. Guthrie, Douglas L. Riegert-Johnson

“…To describe the use of an advanced genetic testing technique, whole exome sequencing, to diagnose a patient and their family with a SCN9A channelopathy. …”
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