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Bioinformatics combining machine learning and single-cell sequencing analysis to identify common mechanisms and biomarkers of rheumatoid arthritis and ischemic heart failure
Published 2025-01-01“…This study analyzed RNA sequencing data from five RA and IHF datasets to identify common biological mechanisms and significant biomarkers. …”
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Next-Generation Sequencing Analysis on Image-Guided Biopsy Samples in Early-Stage Lung Cancer: Feasibility Study and Comparison With Surgical Samples
Published 2025-02-01“…Introduction: Limited information exists on next-generation sequencing (NGS) success for lung tumors of 30 mm or less. …”
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Plasma mcfDNA Sequencing May Improve Usual Care Diagnostics to Detect HHV-8 Among Outpatient People with Advanced HIV
Published 2025-02-01“…The potential added benefit of the detection of other pathogens by mcfDNA sequencing may be particularly relevant for this population. …”
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Combined metabolomics and 16S rDNA sequence analyses of the gut microbiome reveal the action mechanism of Fructus Akebiae against hepatic fibrosis
Published 2025-02-01Subjects: Get full text
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Enantioselective Assembly of Fully Substituted α‐Amino Allenoates Through a Mannich Addition and Stepwise [3,3]‐σ Rearrangement Sequence
Published 2025-01-01“…Control experiments and the density functional theory (DFT) calculations indicated that this cascade reaction involves a Mannich‐type addition and stepwise [3,3]‐σ rearrangement sequence, leading to the fully substituted α‐amino allenoates, which are elusive and take multi‐step to prepare with other methods, in high yields and excellent enantioselectivity.…”
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3′ Splice Site Sequences of Spinal Muscular Atrophy Related SMN2 Pre-mRNA Include Enhancers for Nearby Exons
Published 2014-01-01“…In order to understand the functions of splice site sequences on exon 7 and 8, we analyzed the effects of conserved splice site sequences on exon 7 skipping of SMN2 and SMN1 pre-mRNA. …”
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Application of metagenomic next-generation sequencing in the diagnosis of post-stroke infections: a case series study using multiple sample types
Published 2025-01-01Subjects: “…next-generation sequencing…”
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Whole Exome Sequencing and Molecular Modeling of a Missense Variant in TNFAIP3 That Segregates with Disease in a Family with Chronic Urticaria and Angioedema
Published 2018-01-01“…Interestingly, both the individual’s 41-year-old sister and 12-year-old daughter were also affected with chronic urticaria and severe angioedema. Whole exome sequencing of the proband and several family members revealed a heterozygous variant of uncertain significance in exon 2 of TNFAIP3, denoted as c.65G>A (p.R22Q), in all affected members. …”
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Biological effectiveness of combined exposure to neutrons and gamma radiation applied in two orders of sequence: Relevance for biological dosimetry after nuclear emergencies
Published 2025-02-01“…Conclusions: The results demonstrate that combined exposure to neutrons and gamma radiation, regardless of the order of sequence, leads to an additive response at both endpoints. …”
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Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew.
Published 2025-01-01“…This assay relies on the selection of a segregating, haploid F1 progeny population on a resistant host, followed by bulk sequencing, thereby allowing rapid avirulence candidate gene identification with high mapping resolution. …”
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Integrated RNA sequencing analysis and machine learning identifies a metabolism-related prognostic signature in clear cell renal cell carcinoma
Published 2025-01-01Subjects: Get full text
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Whole-Exome Sequencing Identifies One De Novo Variant in the FGD6 Gene in a Thai Family with Autism Spectrum Disorder
Published 2018-01-01“…The objective of this study is to identify causative variants of autism in two Thai families by using whole-exome sequencing technique. Whole-exome sequencing was performed with autism-affected children from two unrelated families. …”
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COMPARISON OF THE COMPLETE NUCLEOTIDE SEQUENCES OF MITOCHONDRIAL DNA FROM WISTAR AND OXYS RAT STRAINS MAINTAINED AT THE INSTITUTE OF CYTOLOGY AND GENETICS SB RAS
Published 2015-01-01“…The nucleotide sequences were submitted to GenBank. It was shown that two additional unique nucleotide substitutions between rats OXYS (ICG) and Wistar (ICG) were absent from other known nucleotide sequences of rat mitochondrial DNA of rats and that they did not cause amino acid substitutions, so that all phenotypic differences between the lines were determined only by differences in their nuclear DNA. …”
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Virulence-associated variants in Cryptococcus neoformans sequence type 93 are less likely to be associated with population structure compared to independent rare mutations
Published 2025-01-01“…In this study, we examined the relationship between two subpopulations in the sequence type 93 clade of C. neoformans: ST93A and ST93B. …”
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Hydrocarbon potential in the Northern Egyptian Red Sea: insights from geophysical datasets and analysis of onshore marginal outcrop analogues and subsurface sequences
Published 2025-01-01“…Most offshore boreholes in the Red Sea bottomed in Precambrian rock directly below the syn-rift Miocene sequence, suggesting these wells were off-structure, similar to sidetracked wells in the Gulf of Suez, which successfully captured thick pre- and syn-rift sediments. …”
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Effect of Welding Sequence and Constraint on the Residual Stress and Deformation of Thick Welded Butt Joint Made of Q345qD Steel
Published 2022-01-01“…After the finite element model was verified against the experimental results, the effects of five welding sequences and two types of boundary constraint conditions on the residual stress and deformation of the thick welded butt joints were investigated. …”
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