Identification of methyltransferase and demethylase genes and their expression profiling under biotic and abiotic stress in pigeon pea (Cajanus cajan [L.] Millspaugh) by Priyanka Kumari, Priyanka Kumari, Sougata Bhattacharjee, Sougata Bhattacharjee, K. Venkat Raman, Jyotsana Tilgam, Jyotsana Tilgam, Krishnayan Paul, Krishnayan Paul, Kameshwaran Senthil, Mahi Baaniya, Mahi Baaniya, G. Rama Prashat, Rohini Sreevathsa, Debasis Pattanayak

“…Blast search, using Arabidopsis gene sequences, resulted in the identification of two methylation genes (CcMTA70, CcMTB70), two genes encoding adaptor proteins for methylation (CcFIPA and CcFIPB) and 10 demethylase (ALKBH) genes (CcALKBH1A, CcALKBH1B, CcALKBH1C, CcALKBH2, CcALKBH8, CcALKBH8A, CcALKBH8B, CcALKBH9, CcALKBH10A and CcALKBH10B) in the pigeon pea genome. …”
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Late-stage diversification strategy for the synthesis of peptide acids and amides using hydrazides by Shoko Tanaka, Mizuki Kanno, Yosuke Tashiro, Tetsuo Narumi, Nobuyuki Mase, Kohei Sato

“…Azidation and subsequent treatment with BME afforded peptide acids an acceptable yield even in sequences containing amino acids that are prone to oxidation. …”
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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress by Yong-Juan Mo, Yi-Li Gao, Shi-Jjian Xu, Bin Wang, Jingping Wu, Peng Yu, Ming Li, Xiangtao Kang, Zhi-Qiang Han

“…The cDNA encoding the Russian sturgeon protein Atrogin-1 was successfully cloned, comprising 1472 bp and encoding 353 amino acid residues (with an open reading frame of 1062 bp). Utilizing the sequence of Russian sturgeon atrogin-1, we designed novel primers and a specific anti-peptide antibody for our study, confirming that atrogin-1 is selectively expressed in skeletal muscle and heart tissues. …”
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Biparametric MRI-based radiomics for noninvastive discrimination of benign prostatic hyperplasia nodules (BPH) and prostate cancer nodules: a bio-centric retrospective cohort study by Yangbai Lu, Runqiang Yuan, Yun Su, Zhiying Liang, Hongxing Huang, Qu Leng, Ang Yang, Xuehong Xiao, Zhaoqi Lai, Yongxin Zhang

“…A total of 1130 radiomic features were extracted from each MRI sequence, including shape-based features, gray-level histogram-based features, texture features, and wavelet features. …”
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5′-UTR allelic variants and expression of the lycopene-ɛ-cyclase <i>LCYE</i> gene in maize (<i>Zea mays</i> L.) inbred lines of Russian selection by D. Kh. Arkhestova, B. R. Shomakhov, A. V. Shchennikova, E. Z. Kochieva

“…The parallel β,ε-branch is triggered by lycopene-ε-cyclase LCYE, a low expression of which leads to an increase in provitamin A content and is associated with the variability    of the 5’-UTR gene regulatory sequence. In this study, we screened a collection of 165 maize inbred lines of Russian selection for 5’-UTR LCYE allelic variants, as well as searched for the dependence of LCYE expression levels on the 5’-UTR allelic variant in the leaves of 14 collection lines. 165 lines analyzed were divided into three groups carrying alleles A2 (64 lines), A5 (31) and A6 (70), respectively. …”
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A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer by Sooyeon Oh, Sang-Soo Lee, Hoeyoung Jin, Seo-Hyeon Choi, Choong-Keun Cha, Jooho Lee, KyuBum Kwack, Sang Gyun Kim, Sang-Woon Choi

“…As a proof-of-concept pilot study, the expression of ADAM8, ADAM9, ADAM10, ADAM12, ADAM17, and major histocompatibility complex (MHC) class I chain-related sequence A (MICA), a ligand for NKG2D, in gastric cancer was investigated in silico using The Cancer Genome Atlas (TCGA) database. …”
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Effectiveness of a Virtual Reality Serious Video Game (The Secret Trail of Moon) for Emotional Regulation in Children With Attention-Deficit/Hyperactivity Disorder: Randomized Clin... by Marina Martin-Moratinos, Marcos Bella-Fernández, María Rodrigo-Yanguas, Carlos González-Tardón, Chao Li, Ping Wang, Ana Royuela, Pilar Lopez-Garcia, Hilario Blasco-Fontecilla

“…MethodsThis was a prospective, unicentric, randomized, unblinded, pre- and postintervention study with block-randomized sequence masking. Participants included individuals aged between 7 and 18 years who had a clinical diagnosis of ADHD and were receiving pharmacological treatment. …”
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Time-Series Forecasting of PM_2.5 and PM₁₀ Concentrations Based on the Integration of Surveillance Images by Yong Wu, Xiaochu Wang, Meizhen Wang, Xuejun Liu, Sifeng Zhu

“…Our model, which combines a single-channel hybrid network consisting of VGG16 and LSTM (named VGG16-LSTM) with a single-channel Long Short-Term Memory (LSTM) network, efficiently captures detailed spatiotemporal features from surveillance image sequences and temporal features from atmospheric, meteorological, and temporal data, enabling accurate time-series forecasting of PM_2.5 and PM₁₀ concentrations. …”
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Impact of non-invasive oxygen reserve index versus standard SpO2 monitoring on peripheral oxygen saturation during endotracheal intubation in the intensive care unit: Protocol for... by Hugo Hille, Aurélie Le Thuaut, Pierre Asfar, Quentin Quelven, Emmanuelle Mercier, Anthony Le Meur, Jean-Pierre Quenot, Virginie Lemiale, Grégoire Muller, Martin Cour, Alexis Ferré, Asael Berge, Anaïs Curtiaud, Maxime Touron, Gaetan Plantefeve, Jean-Charles Chakarian, Jean-Damien Ricard, Gwenhael Colin, Arthur Orieux, Patrick Girardie, Mathieu Jozwiak, Manon Rouaud, Camille Juhel, Jean Reignier, Jean-Baptiste Lascarrou, CRICS-TRIGGERSEP Network

“…Baseline parameters, rapid-sequence induction medications, ETI devices, and physiological data are recorded. …”
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CRISPR/Cas12a-based method coupled with isothermal amplification to identify Alternaria spp. isolated from wheat grain samples by Aisha Shaizadinova, Aisha Shaizadinova, Meruyert Amanzholova, Meruyert Amanzholova, Irina Rukavitsina, Sailau Abeldenov, Anuar Rysbekovich Zhumakayev

“…Isothermal amplification methods, including nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA), enable nucleic acid amplification at constant temperatures, making them ideal for point-of-care diagnostics without the need for thermal cycling equipment. …”
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An association between type A porcine endogenous retrovirus copy number and hematological parameters and gender in miniature pigs by R. B. Aitnazarov, S. V. Nikitin, G. V. Kontsevaya, M. I. Voevoda, N. S. Yudin

“…Based on the nucleotide sequence of the envelope gene (env), three main types of pig retrovirus, PERV-A, PERV-B and PERV-C, have been recognized, with PERV-A and PERV-B having the capability of infecting human cell lines in vitro. …”
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Predicting the Most Deleterious Missense Nonsynonymous Single-Nucleotide Polymorphisms of Hennekam Syndrome-Causing CCBE1 Gene, In Silico Analysis by Khyber Shinwari, Liu Guojun, Svetlana S. Deryabina, Mikhail A. Bolkov, Irina A. Tuzankina, Valery A. Chereshnev

“…Structural homology-based methods and sequence homology-based tools revealed that 8.8% of the nsSNPs are pathogenic. …”
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High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren by Xinyi Jiang, Jiacheng Shen, Peng Lin, Youming Hou

“…To understand whether the highly expressed CYPs in the antennae play an olfactory function in <i>Solenopsis invicta</i> worker, in this study, we find six highly expressed antennal CYPs from the transcriptome of <i>S. invicta</i>. Multiple sequence alignment and phylogenetic analysis divided them into two families: the CYP3 family (<i>SinvCYP6K1</i>, <i>SinvCYP6K1-1</i>) and the CYP4 family (<i>SinvCYP4C1</i>, <i>SinvCYP4C1-1</i>, <i>SinvCYP4C1-2</i>, <i>SinvCYP4V2</i>). …”
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Phylogenomic analysis reveals exceptions to the co-evolution of ZAR1 and ZRK immune gene families in plants by Li Yang, Shengyi Liu, M. Eric Schranz, Klaas Bouwmeester

“…Conclusion This study provides novel findings on the evolution of ZAR1 and ZRK immune gene families across a wide range of plant species, suggesting that more potential exceptions can be expected when expanding the list of sequenced genomes from distinct orders. Our results provide new hypotheses about the origin and diversification of these critical immune genes for future functional studies.…”
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Mechanism of <italic>Hindu Datura</italic> in the Treatment of Rheumatoid Arthritis from the Perspective of Network Pharmacology by Changlong FU, Yangyang MEI, Xihai LI, Zhiwei QIU, Changxing CHEN, Chunsong ZHENG

“…Objective:To construct the active component target of <italic>Hindu datura</italic> and the disease target of rheumatoid arthritis by using network pharmacology, to predict the specific molecular signal and pathway of <italic>hindu datura</italic> in the treatment of rheumatoid arthritis, and to analyze its potential therapeutic target and molecular mechanism.Methods:All the chemical components of <italic>hindu datura</italic> were summarized and screened through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database, retrieval was performed with the preset oral bioavailability(OB)≥30%and drug like(DL)≥0.18.Uniprot database was used to screen the entire correlation basis of the effective chemical compound.After screening the disease target with"rheumatoid arthritis"as the retrieval word using GeneCards and OMIM database, the superimposed target area between the drug target of <italic>hindu datura</italic> and the disease target of rheumatoid arthritis was summarized and analyzed, and it could be used as the prediction target gene of <italic>hindu datura</italic> for treating rheumatoid arthritis.Network targets were constructed and analyzed for the exported data based on Cytoscape 3.7.1 software and STRING 7.8 database.Finally, GO functional enrichment analysis and KEGG signal pathway analysis were performed to further screen the main targets and signal pathways.Results:①Through screening and analysis, 27 effective compounds, 318 corresponding target genes and 125 predicted target genes were obtained for the treatment of rheumatoid arthritis.②The core genes in PPI network mainly included JUN, AKT1, RELA, MAPK1, etc.③GO functional enrichment analysis showed that the biological process(BP)for the treatment of rheumatoid arthritis by <italic>hindu datura</italic> mainly involves response to lipopolysaccharide, response to lipopolysaccharide, response to molecule of bacterial origin, response to metalion, response to oxidative stress, response to steroid hormone, cellular response to oxidative stress, reactive oxygen species metabolic process, response to oxygen levels, etc..The cell component(CC)consisted of membrane raft, membrane region, plasma membrane raft and presynaptic membrane; molecular functions(MF)mainly involved the nuclear receptor activity, transcription factor activity(direct ligand regulated sequence-specific DNA binding), steroid hormone receptor activity, cytokine receptors, cytokine activity, DNA-binding transcription activator activity, RNA polymeraseⅡtranscription factor binding, kinase regulator activity, receptor ligand activity, etc.The analysis of KEGG signaling pathway showed that the obvious pathway of <italic>hindu datura</italic> in the treatment of rheumatoid arthritis may involve IL-17 signaling pathway, TNF signal pathway, HIF-1signalpathway, Toll-like receptor signal pathway, etc.Conclusion:The potential mechanism of <italic>hindu datura</italic> for the treatment of rheumatoid arthritis is to exert anti-inflammatory, analgesic and antioxidant effects, which involve a number of interactive signal pathways.…”
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The relationship between gallstone disease and the genetic variants of the sterol transporter adenosine triphosphate–binding cassette G8 and G5 in Egyptians by Menna A. Hassan, Salwa M. Abo El-Khair, Noha M. Mesbah, Dina M. Abo-Elmatty, Asmaa R. Abdel-Hamed

“…These polymorphisms result from nucleotide substitutions in the gene sequences, which affect the transporter’s ability to efficiently regulate cholesterol secretion into the bile. …”
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Case Report of Jingyun Platform's Cognitive Training on Cognitive Impairment with Aphasia by Yu ZHOU, Zhuoming CHEN, Hantian GUAN, Chaoping OUYANG

“…Jingyun platform's cognitive training stage was B stage, the cognitive training design was adopted according to the functional impairment of the case, and the automatic, intelligent and interesting training was conducted. The ABBA balance sequence was used for the intervention of the case, and the intervention process was divided into six stages, including pretreatment, traditional cognitive training, Jingyun platform cognitive training, blank stage, Jingyun platform cognitive training and traditional cognitive training. …”
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Multivariate description of gait changes in a mouse model of peripheral nerve injury and trauma. by Bilal A Naved, Shuling Han, Kyle M Koss, Mary J Kando, Jiao-Jing Wang, Craig Weiss, Maya G Passman, Jason A Wertheim, Yuan Luo, Zheng J Zhang

“…<h4>Methods</h4>DigiGait data was collected of mice across groups representing increasing degrees of damage to the neuromusculoskeletal sequence of gait; that is (a) healthy controls, (b) nerve damage only via total nerve transection + reconnection of the femoral and sciatic nerves, and (c) nerve, muscle, and bone damage via total hind-limb transplantation. …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…For T cell repertoireβ-CDR3 analyses, T cell CDR3 was sequenced from gDNA isolated from splenocytes and enzymatically digested tumors.Results We report that activation of STING within the TME leads to slowed melanoma growth in association with increased production of antiangiogenic factors including Tnfsf15 (Vegi) and Cxcl10, and TLS-inducing factors including Ccl19, Ccl21, Lta, Ltb and Light. …”
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Multiple Controls on the Accumulation of Organic-Rich Sediments in the Besa River Formation of Liard Basin, British Columbia, Canada by Jiyoung Choi, Hyun Suk Lee, Yuri Kim, Omid H. Ardakani, Sung Kyung Hong

“…The Late Devonian Besa River Formation is an organic-rich shale sequence in Liard Basin, northeastern British Columbia, Canada, with significant natural gas reserves. …”
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