Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt by Ahmed A. Ali, Rasha A. M. Azouz, Nahla A. Hussein, Reem El-Shenawy, Naiera M. Helmy, Yasmine S. El-Abd, Ashraf A. Tabll

“…Methods Lentiviral-based vectors were constructed and employed to integrate the full-length sequence of Core, E1, E2, and P7 genes of HCV genotype 4 into the genome of Human Embryonic Kidney cells (HEK293T). …”
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A novel pulse-current waveform circuit for low-energy consumption and low-noise transcranial magnetic stimulation by Xinhua Tan, Ao Guo, Jiasheng Tian, Yingwei Li, Yingwei Li, Jian Shi

“…Finally, four insulated-gate bipolar transistors, controlled by a series of PWM pulse sequences, generated the desired pulse-current duration and direction in the H-bridge circuit. …”
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Genome-wide identification of CAMTA gene family in teak (Tectona grandis) and functional characterization of TgCAMTA1 and TgCAMTA3 in cold tolerance by Wenlong Zhou, Jian Du, Runjie Jiao, Xianbang Wang, Tiansong Fang, Guihua Huang

“…On the basis of protein sequence homology, the CAMTA family could be divided into three subgroups. …”
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Cytokinin Oxidase (CKX) Family Members in ‘duli’ (<i>Pyrus betulifolia Bunge</i>): Genome-Wide Identification and Tissue Expression Profile Under Abiotic Stress by Weimin Wang, Ting Xie, Jiaojiao He, Kuozhen Nie, Zijuan He, Yuxing Zhang, Yingli Li

“…A total of 10 <i>PbCKX</i> genes were identified, which are unevenly distributed across five chromosomes and classified into four groups based on sequence similarity and phylogenetic relationships. …”
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Impact of cooling diabetic patients’ fingertips compared to vibrating stimulation on pain from the glucometer needle: A cross-over trial study by Vahid Vejdanihemmat, Hiva Azami, Leili Tapak, Shiva Borzouei, khodayar Oshvandi

“…The study design consisted of six blocks, each containing three interventions: vibration stimulation (A), cold therapy (B), and a combination of both C. The sequences of interventions (ABC, ACB, BCA, BAC, CBA, and CAB) were randomly assigned. …”
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A maChine and deep Learning Approach to predict pulmoNary hyperteNsIon in newbornS with congenital diaphragmatic Hernia (CLANNISH): Protocol for a retrospective study. by Ilaria Amodeo, Giorgio De Nunzio, Genny Raffaeli, Irene Borzani, Alice Griggio, Luana Conte, Francesco Macchini, Valentina Condò, Nicola Persico, Isabella Fabietti, Stefano Ghirardello, Maria Pierro, Benedetta Tafuri, Giuseppe Como, Donato Cascio, Mariarosa Colnaghi, Fabio Mosca, Giacomo Cavallaro

“…A retrospective data collection of clinical and radiological variables from newborns' and mothers' clinical records will be performed for eligible patients born between 01/01/2012 and 31/12/2020. The native sequences from fetal magnetic resonance imaging (MRI) will be collected. …”
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Preliminary analysis of the role of small hepatitis B surface proteins mutations in the pathogenesis of occult hepatitis B infection via the endoplasmic reticulum stress-induced UP... by Huang Chengrong, Zhang Hao, Wang Jing, Li Jianfei, Liu Qian, Zong Qiyin, Zhang Yunyun, Wang Qin, Zhou Qiang

“…Huh7 and HepG2 cells were transfected with plasmids encoding wild-type (WT) or OBI-related SHB mutation-containing sequences, after which a chemiluminescence approach was used to detect HBsAg levels in cell culture supernatants. …”
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Urethra-sparing prostate cancer radiotherapy: Current practices and future insights from an international survey by Jennifer Le Guévelou, Paul Sargos, Piet Ost, Filippo Alongi, Stefano Arcangeli, Alejandro Berlin, Pierre Blanchard, Anna Bruynzeel, Olivier Chapet, Alan Dal Pra, Robert T. Dess, Matthias Guckenberger, Andrew Loblaw, Amar U. Kishan, Barbara Alicja Jereczek-Fossa, David Pasquier, Mohamed Shelan, Shankar Siva, Alison C. Tree, Costantinos Zamboglou, Stephane Supiot, Vedang Murthy, Thomas Zilli

“…Results: Magnetic resonance imaging with T2-weighted sequences is the preferred method for urethra contouring (83 % consensus). …”
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AmelOBP4: an antenna-specific odor-binding protein gene required for olfactory behavior in the honey bee (Apis mellifera) by Fang Liu, Yu Lai, Lixian Wu, Qiang Li, Linyue Lei, Wei Yin, Yuan Zhang, Zachary Y. Huang, Hongxia Zhao

“…RNA interference (RNAi) is the induction of sequence specific gene silencing by double-stranded RNA (dsRNA), it is a powerful tool that makes gene inactivation possible in organisms that were not amenable to genetic analysis before. …”
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Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…First, guided by the sequence of a related clone and the crystal structure of h1340.CC in complex with PD-1, we generated variant h1340.SA.LV with a potency and cross-reactivity similar to h1340.CC, but only partially recovered affinity. …”
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Genomic insights on cgMLST markers, drug resistance, and urease cluster of Proteus mirabilis strains by Shitao Lian, Yadong Liu, Songnian Hu, Chen Shen, Yinping Ma, Peng Yin, Zilong He

“…Additionally, an attempt was made to construct a core genome multilocus sequence typing scheme specific to this species in order to enhance the precision of describing genetic diversity and evolutionary relationships. …”
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Chloroquine and Rapamycin Augment Interleukin-37 Expression via the LC3, ERK, and AP-1 Axis in the Presence of Lipopolysaccharides by Xiaoyi Shi, Chunhui Lai, Lianyu Zhao, Mingying Zhang, Xi Liu, Shanqin Peng, Weizhong Guo, Qiuying Xu, Song Chen, Guang-xing Chen

“…AP-1 and p65 binding motifs could be deduced in the sequence of the IL-37 promoter. Inductive IL-37 expression was accompanied with increased phosphorylated Erk1/2 and AP-1 and could be completely abolished by an Erk1/2 inhibitor or augmented by Erk1/2 agonists. …”
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Oleanolic Acid Induces Differentiation of Neural Stem Cells to Neurons: An Involvement of Transcription Factor Nkx-2.5 by You Ning, Jianhua Huang, Bill Kalionis, Qin Bian, Jingcheng Dong, Junzhen Wu, Xiantao Tai, Shijin Xia, Ziyin Shen

“…Through transcription factor binding site analyses of the upstream regulatory sequences of these genes, 87 genes were predicted to share a common motif for Nkx-2.5 binding. …”
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Study protocol for a stepped-wedge implementation study investigating the intersectoral collaboration of implementing the TREAT INTERACT intervention for primary school teachers an... by Ane-Marthe Solheim Skar, Ingunn Marie S. Engebretsen, Nora Braathu, Harriet Aber, Harald Bækkelund, Melf-Jakob Kühl, Marjorie Mukisa, Joyce Sserunjogi Nalugya, Norbert Skokauskas, Vilde Skylstad, Tore Wentzel-Larsen, TREAT research consortium, Juliet Ndimwibo Babirye

“…The main study utilize a stepped-wedged trial design with six starting sequences, randomizing three schools to the intervention at each interval, while the remaining act as “controls”. …”
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Biogenic synthesis of titanium nanoparticles by Streptomyces rubrolavendulae for sustainable management of Icerya aegyptiaca (Douglas) by Enayat M. Elqady, Eman El-said, Asmaa A. Tharwat, Lina A. Abou El-Khashab, Inas M. Y. Mostafa, Fatma Z. Hamed, Wesam M. Morsi, Mohamed M. Rezk, Inas M. Abou El-Enain

“…In all, seven actinobacterial isolates, the most potent of which has the potential to produce titanium hydroxide-based nanoparticles (TiO2-NP2), have DNA sequences that are 99.9% like those of Streptomyces rubrolavendulae (MCN2) according to nucleotide alignment and a phylogenetic tree. …”
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Genomic scans for selection and runs of homozygosity in southern Italian turkey populations by Medhat S. Saleh, Vincenzo Landi, Martijn F.L. Derks, Gerardo Centoducati, Martien A.M. Groenen, Pasquale De Palo, Elena Ciani, Nicola Pugliese, Elena Circella, Antonio Camarda

“…A total of 73 BAS-APU turkeys from five populations were sequenced (12X). The inbreeding coefficients based on ROH ranged from 0.177 to 0.405. …”
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Trivalent recombinant protein vaccine induces cross-neutralization against XBB lineage and JN.1 subvariants: preclinical and phase 1 clinical trials by Jingyun Yang, Weiqi Hong, Huashan Shi, Cai He, Hong Lei, Yanan Zhou, Hao Yang, Aqu Alu, Zimin Chen, Yun Yang, Wenhai Yu, Cong Tang, Junbin Wang, Bai Li, Qing Huang, Jiong Li, Li Yang, Wei Wang, Guobo Shen, Jinliang Yang, Zhiwei Zhao, Xiangrong Song, Zhaoming Su, Yuquan Wei, Qiangming Sun, Shuaiyao Lu, Zhenling Wang, Youchun Wang, Guangwen Lu, Weimin Li, Xiawei Wei

“…In this study, we produce three recombinant trimeric proteins from the RBD sequences of Delta, BA.5, and XBB.1.5, formulating a trivalent vaccine (Tri-Vac) with an MF59-like adjuvant at a 1:1:4 ratio. …”
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Repressor Element 1 Silencing Transcription Factor (REST) Governs Microglia-Like BV2 Cell Migration via Progranulin (PGRN) by Tongya Yu, Yingying Lin, Yuzhen Xu, Yunxiao Dou, Feihong Wang, Hui Quan, Yanxin Zhao, Xueyuan Liu

“…To more comprehensively examine the molecular basis, we analyzed the promoter sequence of PGRN and found that it has the potential binding site of REST. …”
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Comparative genomics reveals low levels of inter- and intraspecies diversity in the causal agents of dwarf and common bunt of wheat and hint at conspecificity of Tilletia caries an... by Somayyeh Sedaghatjoo, Bagdevi Mishra, Monika K. Forster, Yvonne Becker, Jens Keilwagen, Berta Killermann, Marco Thines, Petr Karlovsky, Wolfgang Maier

“…The gene content as well as intraspecies variation in these species and the genetic basis of their separation is unknown. We sequenced the genome of four T. caries, five T. controversa, and two T. laevis and extended this dataset with five publicly available ones. …”
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Alternative silencing states of transposable elements in Arabidopsis associated with H3K27me3 by Valentin Hure, Florence Piron-Prunier, Tamara Yehouessi, Clémentine Vitte, Aleksandra E. Kornienko, Gabrielle Adam, Magnus Nordborg, Angélique Déléris

“…We also show that H3K27me3 can be deposited on newly inserted transgenic TE sequences in a TE-specific manner indicating that silencing is determined in cis. …”
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