Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) by Jiangling Li, Jingjie Liang, Mengyang Wang, Yuewen Jiang, Wen Li, Mingxi Huang, Yan Huang, Yangjie Xie, Jianchun Chen, Tiansheng Chen

“…A total of 24,661 unigenes were identified in ovaries and 15,023 in testes, along with genomic regulatory elements such as transcription factors, simple sequence repeats, and long non-coding RNAs. Additionally, 1,210 differentially expressed genes were detected. …”
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Real-World Outcomes of Pralsetinib in RET Fusion-Positive NSCLC by Francesca Lucibello, MD, Valérie Gounant, MD, PhD, Mihaela Aldea, MD, PhD, Michaël Duruisseaux, MD, PhD, Maurice Perol, MD, Christos Chouaid, MD, Jaafar Bennouna, MD, PhD, Vincent Fallet, MD, Aldo Renault, MD, Florian Guisier, MD, PhD, Etienne Giroux-Leprieur, MD, PhD, Marie Wislez, MD, PhD, Anne-Claire Toffart, MD, PhD, Julien Mazieres, MD, PhD, Clémence Basse, MD, PhD, Nadia Hegarat, PhD, Matthieu Carton, MD, Nicolas Girard, MD, PhD

“…Objective: To assess real-world efficacy of pralsetinib and treatment sequences in patients with RET fusion-positive NSCLC. …”
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Developing a codesigned text message-based digital oral health education resource (TOOTH) by Shalinie King, Lauren Adriel Church, Edel O’Hagan, Dion Candelaria, Aboli Pawar, Ashley Cooper, Rebecca Chen, Alice Gibson

“…The final resource developed includes 81 text messages sequenced to ensure a gradual increase in knowledge and skill and five supporting videos. …”
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Isolation and identification of novel casein-derived bioactive peptides and potential functions in fermented casein with Lactobacillus helveticus by Mengzhu Fan, Tingting Guo, Wanru Li, Jing Chen, Fushuo Li, Chao Wang, Yi Shi, David Xi-an Li, Shaohui Zhang

“…Intracellular peptides are much larger in quantity and more complex in composition than extracellular peptides, between which the correlation reveals proteolytic and metabolic mechanisms.In addition, totally 241 different peptide sequences were identified by Nano LC–MS/MS from casein (212) and Lactobacillus helveticus proteins (29). …”
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Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…Ultimately, the detection capability of this method was assessed using clinical samples.ResultsThe sequenced tags, when paired with primers, could generate Tm differences exceeding 3°C. …”
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The GWAS-MAP|ovis platform for aggregation and analysis of genome-wide association study results in sheep by A. V. Kirichenko, A. S. Zlobin, T. I. Shashkova, N. A. Volkova, B. S. Iolchiev, V. A. Bagirov, P. M. Borodin, L. С. Karssen, Y. A. Tsepilov, Y. S. Aulchenko

“…The platform currently contains information on more than 34 million associations between genomic sequence variants and traits of meat production in sheep. …”
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Late-onset first epileptic seizure and cerebral small vessel disease: role of juxtacortical white matter lesions by Adrian Nasca, Adrian Nasca, Leo Sokolovič, Leo Sokolovič, Oliver Koprda, Patrick Haage, Patrick Haage, Thorsten Schmidt, Juraj Kukolja, Juraj Kukolja

“…A voxel-based morphometry (VBM) analysis was additionally performed on T1 weighted sequences using the automated SPM12 based CAT12 software.ResultsComparing intrapersonal volume ratios adjusted for the effects of gender and age, we found that the WML distribution was shifted to the juxtacortical compartment in LOFES patients. …”
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The sarcoma ring trial: a case-based analysis of inter-center agreement across 21 German-speaking sarcoma centers by Siyer Roohani, Jolina Handtke, Kamal Hummedah, Markus Albertsmeier, Dimosthenis Andreou, Leonidas Apostolidis, Marinela Augustin, Sebastian Bauer, Moritz Billner, Florian Bösch, Christoph K. W. Deinzer, Niklas Deventer, Anna Duprée, Franziska Eckert, Lars Engel, Katja Fechner, Hagen Fritzsche, Verena Gaidzik, Saeed Ghani, Robert Grützmann, Wiebke K. Guder, Rainer Hamacher, Judith S. Hecker, Anne Hendricks, Axel Hillmann, Philipp Houben, Georg Hübner, Philipp Ivanyi, Christina Jentsch, Maren Jordan, Peter Kappl, Moritz Kaths, Torsten Kessler, Johanna Kirchberg, Carolin Knebel, Robert Krempien, Burkhard Lehner, Ulrich Lenze, Lars H. Lindner, Alisa Martina Lörsch, Nadia Maguire, Sophie Müller, Pompiliu Piso, Vlatko Potkrajcic, Peter Reichardt, Stephan Richter, Simone Schewe, Lars M. Schiffmann, Felicitas Scholten, Jana Käthe Striefler, Matthias Schwarzbach, Katharina Seidensaal, Sabine Semrau, Joanna Szkandera, Christoph J. Szuszies, Beate Timmermann, Armin Tuscherer, Armin Wiegering, Moritz T. Winkelmann, David Kaul, Jens Jakob

“…Centers provided recommendations on treatment sequence and modalities, along with the consensus level within their MTB. …”
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Cell Wall Protein 2 as a Vaccine Candidate Protects Mice Against <i>Clostridioides difficile</i> Infection by Shaohui Wang, Joshua Heuler, Jessica Bullock, Junling Qin, Soumyadeep Chakraborty, Agbendeh Lubem Nathaniel, Shifeng Wang, Xingmin Sun

“…Results: Our in silico analyses predicted the immunogenic region (functional domain) of Cwp2 and revealed its high homology among various toxinotypes and ribotypes (R.T.s) or sequence types (S.T.s). Immunizations of mice with the Cwp2 functional domain (Cwp2_A) induced potent IgG/A antibody responses against Cwp2_A, protected mice from CDI, and reduced <i>C. difficile</i> spore and toxin levels in feces post-infection. …”
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Discovery of a broad-spectrum monoclonal antibody recognizing a conserved, linear epitope WFYDGYPT on VP1 protein of Enterovirus A species by Lie Fu, Wei-Ping Jin, Wen-Hui Wang, Chen Wang, Sha-Sha Qian, Meng-Jun Wang, Rui-Lun Liu, Song-Zhuang Li, Ya-Xin Du, Sheng-Li Meng, Jing Guo, Ze-Jun Wang, Xiao-Qi Chen, Shuo Shen

“…BLAST of the epitope in the NCBI genus Enterovirus protein database indicates that the epitope sequence is highly conserved among Enterovirus A species, but not among the other Enterovirus species. …”
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A lipidated peptide derived from the C-terminal tail of the vasopressin 2 receptor shows promise as a new β-arrestin inhibitor by Rebecca L. Brouillette, Christine E. Mona, Michael Desgagné, Malihe Hassanzedeh, Émile Breault, Frédérique Lussier, Karine Belleville, Jean-Michel Longpré, Michel Grandbois, Pierre-Luc Boudreault, Élie Besserer-Offroy, Philippe Sarret

“…To address this issue, we designed and synthesised a novel lipidated phosphomimetic peptide inhibitor targeting β-arrestins, called ARIP, which was developed based on the C-terminal tail (A343-S371) of the vasopressin V2 receptor. As the V2R sequence has been shown to bind β-arrestins with high affinity, we added an N-terminal palmitate residue to allow membrane tethering and cell entry. …”
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Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia) by Muhammad Mudasser Nazir, Muhammad Mazhar Ayaz, Atif Nisar Ahmed, Azhar Maqbool, Kamran Ashraf, Muhammad Oneeb, Ghulam Yasin, Atta Subhani, Muhammad Asif Ali, Noman Nazir, Muhammad Afzal Sajid

“…Two of the positive Neospora caninum amplicons were sequenced and had 97% nucleotide identity with N. caninum isolates. …”
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A machine learning-based radiomics approach for differentiating patellofemoral osteoarthritis from non-patellofemoral osteoarthritis using Q-Dixon MRI by Liangjing Lyu, Jing Ren, Wenjie Lu, Jingyu Zhong, Yang Song, Yongliang Li, Weiwu Yao

“…Radiomics features were extracted from Q-Dixon MRI sequences to distinguish PFOA from non-PFOA. The diagnostic performance of the three models was compared using the area under the curve (AUC) values analyzed with the Delong test. …”
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Case report: Deciphering the clinical significance of a novel partial BRCA1 exon 10 duplication in a patient with triple-negative breast cancer by Alice Faversani, Debora Manuelli, Davide Barteselli, Giulia Melloni, Carlo Santaniello, Luigi Corsaro, Luigi Corsaro, Davide Sacco, Davide Sacco, Davide Clerici, Laura Gargiulo, Fulvio Ferrara, Lucy Costantino

“…RNA analysis allowed to confirm the production of an altered mRNA showing the duplicated sequence. In this way, we were able to assign a clinical significance to the new alteration and classify it as a pathogenic variant. …”
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Heterochrony in orthodenticle expression is associated with ommatidial size variation between Drosophila species by Montserrat Torres-Oliva, Elisa Buchberger, Alexandra D. Buffry, Maike Kittelmann, Genoveva Guerrero, Lauren Sumner-Rooney, Pedro Gaspar, Georg C. Bullinger, Javier Figueras Jimenez, Fernando Casares, Saad Arif, Nico Posnien, Maria D. S. Nunes, Alistair P. McGregor, Isabel Almudi

“…We discovered that the activity of an otd eye enhancer is consistent with the difference in the expression of this gene between species, with the D. mauritiana enhancer sequence driving earlier expression than that of D. simulans. …”
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Gradient disparities in allergy and the gut microbiome among rural, migrant, and urban populations across China by Wanjun Wang, Mo Xian, Ruchong Chen, Jing Li, Lulu Wu

“…Fecal microbiotal composition was also sequenced by 16S rRNA and regression-based analyses with covariate adjustment applied. …”
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Unveiling the Role of <i>GhP5CS1</i> in Cotton Salt Stress Tolerance: A Comprehensive Genomic and Functional Analysis of <i>P5CS</i> Genes by Hui Fang, Xin Gao, Yunhao Wu, Ke Zhang, Ying Wu, Junyi Li, Dongmei Qian, Ruochen Li, Haijing Gu, Teame Gereziher Mehari, Xinlian Shen, Baohua Wang

“…In this study, we identified 40 <i>P5CS</i> genes across four cotton species with diverse sequence lengths and molecular weights. Phylogenetic analysis of 100 <i>P5CS</i> genes from nine species revealed three subgroups, with <i>Gossypium hirsutum</i> closely related to <i>Gossypium barbadense</i>. …”
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UTE MRI technical developments and applications in osteoporosis: a review by Soo Hyun Shin, Hee Dong Chae, Hee Dong Chae, Arya Suprana, Arya Suprana, Saeed Jerban, Eric Y. Chang, Eric Y. Chang, Lingyan Shi, Robert L. Sah, Jeremy H. Pettus, Gina N. Woods, Jiang Du, Jiang Du, Jiang Du

“…This review article discusses using ultrashort echo time (UTE) sequences to evaluate bone composition and structure. …”
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Characteristics of Egg Production and Discovery of the Prolactin Gene in Indigenous Tropical White Leghorn Layer Chickens of Indonesia by N Pratiwi, T Sartika, Komarudin, T Haryati, T Kostaman, N Azizah, A Sukmara, B Brahmantiyo, Isbandi, H Zaenal, H Nuraini, H Khasanah, MF Ridhillah, F Saputra

“…A total of 185 WL chickens (5 batches) were utilized, with 47 DNA samples sequenced using specific primers for the PRL gene’s exons 2 and 5. …”
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Lattice-based interpenetrating phase composite metamaterial fabricated with hybrid material extrusion process for tunable mechanical properties by Ritik Raj, Jung-Ting Tsai, Mayur Jiyalal Prajapati, Jeng-Ywan Jeng

“…Overall, this study presents an innovative fabrication technique for IPC metamaterials, revealing how the strategic placement and stacking sequence of secondary materials within the primary structure significantly influences their mechanical properties.…”
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