Patch-Level and Neighborhood-Dependency Spatial Optimization Method (PNO): Application to Urban Land-Use Planning to Facilitate Both Socio-Economic and Environmental Development in... by Yuhan Cheng, Xiuyuan Zhang, Qi Zhou, Xiaoyan Dong, Shihong Du

“…The PNO represents land-use patterns in a graph structure, quantifies land-use patterns’ impacts on the population, economy, and land surface temperature, defines the spatiotemporal constraints of land-use optimization considering neighborhood-dependency and optimization sequences, and finally optimizes land uses and their spatial layouts based on a multi-objective genetic algorithm. …”
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Manure application increases soil doxycycline and zinc levels and resistance gene abundance by Jian Zhao, Jiangjie He, Yurou Han, Junwei Liang, Yucheng Chen, Yimo Zhang, Jizhen Liu, Qizhong Huang, Weihong Xu

“…Through an 8-year field experiment coupled with 16S rRNA sequence analysis, we explored the dynamics of DOX and Zn contamination and their influence on soil microbial diversity and resistance gene expression. …”
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Мutants of inflorescence development in alfalfa (Medicago sativa L.) by N. I. Dzyubenko, E. A. Dzyubenko

“…M. truncatula and alfalfa share highly conserved nucleotide sequences and exhibit nearly perfect  synteny between the two genomes. …”
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Differentially localizing isoforms of the migraine component calcitonin gene-related peptide (CGRP), in the mouse trigeminal ganglion: βCGRP is translated but, unlike αCGRP, not so... by Sofia Lyng Wæver, Kristian Agmund Haanes

“…Methods We applied immunohistochemistry (IHC) on 15 TGs from three different genotypes of mice; wild type (WT) αCGRP heterozygote (Het) and αCGRP KOs, with a primary antibody targeting the mature neuropeptide sequence of both αCGRP and βCGRP. Subsequently, the localization patterns of the two isoforms were analyzed. …”
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Efficient chimeric mouse production using a novel embryonic stem cell line by G. V. Kontsevaya, N. A. Feofanova, A. G. Menzorov, I. E. Pristyazhnyuk, A. V. Smirnov, N. R. Battulin, L. A. Gerlinskaya

“…Never-theless, there is a need for complex genome modifications, such as large deletions, reporter genes insertion into the 3’ gene regulatory sequence, or site-specific modifications of the genome. …”
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Resistance to viruses of potato: current status and prospects by S. S. Makarova, V. V. Makarov, M. E. Taliansky, N. O. Kalinina

“…For this purpose, natural resistance genes, primarily from wild Solanum species, or virus derived nucleotide sequences have been used as sources of resistance. …”
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Multi-label text classification via secondary use of large clinical real-world data sets by Sai Pavan Kumar Veeranki, Akhila Abdulnazar, Diether Kramer, Markus Kreuzthaler, David Benjamin Lumenta

“…Support vector machines performed better for surgery reports with a sequence length greater than 512, achieving a mean average precision of 0.872 in comparison to 0.840 for fastText, 0.837 for medBERT.de and 0.820 for surgeryBERT.at. …”
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Taxonomic refinement of Bacillus thuringiensis by Nagham Shiekh Suliman, Reza Talaei-Hassanloui, Hamid Abachi, Sadegh Zarei, Ebrahim Osdaghi

“…By October 2023, whole genome sequences (WGS) of 885 bacterial strains were labeled as B. thuringiensis in the NCBI GenBank database. …”
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Epidemic Situation of Brucellosis in Jinzhou City of China and Prediction Using the ARIMA Model by Lulu Wang, Chen Liang, Wei Wu, Shengwen Wu, Jinghua Yang, Xiaobo Lu, Yuan Cai, Cuihong Jin

“…Moreover, stability analysis and sequence tranquilization, model recognition, parameter test, and model diagnostic were also carried out. …”
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Physical-aware model accuracy estimation for protein complex using deep learning method by Haodong Wang, Meng Sun, Lei Xie, Dong Liu, Guijun Zhang

“…All these results suggest that physical-aware information based on the area and orientation of atom-atom and atom-solvent contacts has the potential to capture sequence-structure-quality relationships of proteins, especially in the case of flexible proteins. …”
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Application of palliative care in pain management of terminally ill patients (安宁疗护在临终患者疼痛管理中的应用) by CHEN Mingming (陈明明), REN Li (任丽), JIN Qi (金琦)

“…Methods A total of 50 terminally ill patients with pain were divided into the control group and observation group according to the admission sequence, with 25 cases in each group. Patients in the control group received routine nursing and pain management, and those in the observation group were given palliative care interventions in addition. …”
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Association between mild cognitive impairment and APOE Ɛ4, low education level, and lack of spousal relationship in community-dwelling older persons in Nigeria by Isyaku Gwarzo Mukhtar, Mohamed Mabrouk Elkhashab, Muhammad Ali Salim, Salisu Ahmed Ibrahim, Isyaku Umar Yarube

“…APOE was genotyped from peripherally extracted genomic DNA using the sequence-specific primer polymerase chain reaction with Hhal digestion. …”
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USING A DIDACTIC TOOL WITH IT-SUPPORT FOR TEACHING GEOGRAPHY IN A SPECIAL SCHOOL IN SLOVAKIA by Viktor Hladush, Slávka Bendíková, Barbora Kovačova, Marcela Čarnická

“…As an example, the article briefly highlights the essence of the preparatory stage and the specific sequence of the teacher's work when studying one section «Slovakia». …”
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Endophytic fungi related to the ash dieback causal agent encode signatures of pathogenicity on European ash by Maryam Rafiqi, Chatchai Kosawang, Jessica A. Peers, Lukas Jelonek, Hélène Yvanne, Mark McMullan, Lene R. Nielsen

“…Here, we discover the pathogenicity potential of two newly identified related species of Asian origin, H. koreanus and H. occultus, and one Europe-native related species, H. albidus. We sequence the genomes of all three Hymenoscyphus species and compare them to that of H. fraxineus. …”
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Determination of the Antioxidant Capacity of Germinated and Yeast-Fermented Sweet and Bitter Lupin Seeds and Sprouts via Cyclic Voltammetry Compared to the Spectrophotometric and P... by Danuta Zielińska, Piotr Gulewicz, Małgorzata Kasprowicz-Potocka, Henryk Zieliński

“…Cyclic voltammetry (CV), the scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH⦁), and photochemiluminescence (PCL) assays against superoxide anion radicals were applied to hydrophilic (ACW) and lipophilic (ACL) fractions, and the data obtained were used to calculate the TAC after sequence extraction of the samples with 80% methanol followed by methanol/hexane (4:1; <i>v</i>/<i>v</i>). …”
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Xanthohumol and echinocystic acid induces PSTVd tolerance in tomato by Wenkun Tang, Zhichao Tang, Haiyi Liu, Jinbiao Lu, Qianyun Du, Huan Tian, Jingwei Li

“…We obtained the full‐length genome sequence of previously conserved PSTVd and inoculated it on four genotypes of semi‐cultivated tomatoes selected from a local tomato germplasm resource. …”
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Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR by Ahtisham Fazeel Abbasi, Muhammad Nabeel Asim, Andreas Dengel

“…Abstract The revolutionary CRISPR-Cas9 system leverages a programmable guide RNA (gRNA) and Cas9 proteins to precisely cleave problematic regions within DNA sequences. This groundbreaking technology holds immense potential for the development of targeted therapies for a wide range of diseases, including cancers, genetic disorders, and hereditary diseases. …”
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Genes related to the white face colour pattern in eight Russian cattle breeds by N. S. Yudin, N. M. Belonogova, D. M. Larkin

“…Five genes were found within this interval in the cattle genome, out of which the most likely functional candidate was SLC41A2, with the SNP BovineHD0500019319 found within its intronic sequence. SLC41A2 encodes a magnesium transporter protein. …”
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Investigation of Borrow Pit TEA28 BP3, Fenstanton, Cambridgeshire, UK by W.A. Boismier, E. Allison, C. Ardis, R. Banerjea, C.R. Batchelor, P. Dark, K. Dudgeon, C.P. Green, E. Henderson, J. Ladocha, J. Weinstock, D.S. Young, J.-L. Schwenninger

“…Stratigraphically the succession comprised a sequence of fluvial, floodplain and periglacial colluvial deposits. …”
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Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean by Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith

“…Although CRISPR enzymes have been reported to have high sequence‐specificity, careful design of the editing reagents can also reduce unintended edits at highly homologous sites. …”
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