Combination gemcitabine and PD-L1xCD3 bispecific T cell engager (BiTE) enhances T lymphocyte cytotoxicity against cholangiocarcinoma cells by Methi Wathikthinnakon, Piriya Luangwattananun, Nunghathai Sawasdee, Chutipa Chiawpanit, Vannajan Sanghiran Lee, Piyarat Nimmanpipug, Yingmanee Tragoolpua, Siriphorn Rotarayanont, Thanich Sangsuwannukul, Nattaporn Phanthaphol, Yupanun Wutti-in, Chalermchai Somboonpatarakun, Thaweesak Chieochansin, Mutita Junking, Jatuporn Sujjitjoon, Pa-thai Yenchitsomanus, Aussara Panya

“…Two recombinant PD-L1xCD3 BiTEs (mBiTE and sBiTE contain anti-PD-L1 scFv region from atezolizumab and from a published sequence, respectively) were able to specifically bind to both CD3 on T lymphocytes, and to PD-L1 overexpressed after gemcitabine treatment on CCA (KKU213A, KKU055, and KKU100) cells. mBiTE and sBiTE significantly enhanced T lymphocyte cytotoxicity against CCA cells, especially after gemcitabine treatment, and their magnitudes of cytotoxicity were positively associated with the levels of PD-L1 expression. …”
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Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene by Rong Chen, Chen Chen, Yifei Qin, Jie Liu, Mingming Lei, Huanxi Zhu, Zhendan Shi

“…A classical VDRE-like sequence was identified in the goose AMH promoter by in silico analyses. …”
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Quantitative Assessment of Deep Gray Matter Susceptibility and Correlation With Cognition in Patients With Liver Cirrhosis by Wenjun Wu, Yu Su, Ziji Qin, Jiamin Kang, Dongqiao Xiang, Dingxi Liu, Chuansheng Zheng, E. Mark Haacke, Lixia Wang

“…Methods Thirty cirrhotic patients and 30 age‐, gender‐, and education‐matched controls were imaged using a multiecho gradient‐echo sequence for QSM analysis in a 3T scanner. The susceptibility values were determined for the caudate nucleus (CN), putamen (PU), globus pallidus (GP), thalamus (TH), red nucleus (RN), substantia nigra (SN), and dentate nucleus (DN). …”
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Heterologous vaccination with subunit protein vaccine induces a superior neutralizing capacity against BA.4/5‐included SARS‐CoV‐2 variants than homologous vaccination of mRNA vacci... by Dandan Peng, Tingmei Zhao, Weiqi Hong, Minyang Fu, Cai He, Li Chen, Wenyan Ren, Hong Lei, Jingyun Yang, Aqu Alu, Yanghong Ni, Jian Liu, Jiong Li, Wei Wang, Guobo Shen, Zhiwei Zhao, Li Yang, Jinliang Yang, Zhenling Wang, Yoshimasa Tanaka, Guangwen Lu, Xiangrong Song, Xiawei Wei

“…In the present study, we prepared a Delta full‐length spike protein sequence‐based mRNA vaccine as the “priming” shot and developed a recombinant trimeric receptor‐binding domain (RBD) protein vaccine referred to as RBD–HR/trimer as a third heterologous booster. …”
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G-cleave LC3B biosensor: monitoring autophagy and assessing resveratrol's synergistic impact on doxorubicin-induced apoptosis in breast cancer cells by Chiao-Chun Liao, Yuqing Long, Ming-Lin Tsai, Chun-Yu Lin, Kai-Wen Hsu, Chia-Hwa Lee

“…In this study, we developed a non-invasive autophagy detection system (NIADS-autophagy, also called G-cleave LC3B biosensor) by integrating a split-luciferase-based biosensor with an LC3B cleavage sequence, which swiftly identified classic autophagic triggers, such as Earle's Balanced Salt Solution and serum deprivation, through protease-mediated degradation pathways. …”
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Detection and Phylogenetic Analysis of an Exotic Strain of Porcine Epidemic Diarrhea Virus and Its Effect on an Affected Herd Immunized Against the Endemic Strain in Thailand by Christopher James Stott, Patumporn Jermsutjarit, Pornchai Pornpanom, Hongyao Lin, Angkana Tantituvanont, Dachrit Nilubol

“…Additionally, a novel <b>G2b</b> strain was sequenced for phylogenetic and protein structure analyses. …”
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Magnetic susceptibility controlled by climate-driven weathering intensity by Aubineau Jérémie, Antonio Paul Y.J., El Bamiki Radouan, Parat Fleurice, Camps Pierre, Raji Otmane, Jourani Es-Said, Bodinier Jean-Louis, Macouin Mélina, Gilder Stuart, Rousse Sonia, Séranne Michel

“…Bulk mineralogy, Rietveld refinements of powder XRD patterns, and whole-rock geochemistry, combined with a MS investigation across a ∼290 m-thick Upper Cretaceous/Paleogene sedimentary sequence at Erguita, Morocco, imply that iron is exclusively detrital in origin. …”
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A functional approach to analyze the genetic basis for differences in virulence of monkeypox virus clades by Patricia L. Earl, Jeffrey L. Americo, Sara Reynolds, Wei Xiao, Catherine Cotter, Bernard Moss

“…The present study was intended to evaluate the CAST mouse as a model for investigating genomic differences by replacing genes of Zaire-1979 005 (Z-79) clade Ia MPXV with homologous gene sequences of the less virulent and less transmissible USA-2003 clade IIa MPXV. …”
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Bp-13 PLA2: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom by Georgina Sucasaca-Monzón, Priscila Randazzo-Moura, Thalita Rocha, Frank Denis Torres-Huaco, Augusto Vilca-Quispe, Luis Alberto Ponce-Soto, Sérgio Marangoni, Maria Alice da Cruz-Höfling, Léa Rodrigues-Simioni

“…Amino acid analysis showed a high content of hydrophobic and basic amino acids and 14 half-cysteine residues. The N-terminal sequence showed a high degree of homology with basic Asp49 PLA2 myotoxins from other Bothrops venoms. …”
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Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation by Yiping Wan, Jiannan Zhang, Xiao Zhang, Jiliang He, Ningkun Shi, Yuanyou Li, Juan Li, Yajun Wang

“…Our results showed that: (1) cC5H11ORF96 cDNA encodes a 120 amino acids protein, which shares high sequence identity with that of birds, mammals, reptiles, frogs and fish; (2) cC5H11ORF96 has a fully conserved RFKTQP motif and high proportion of serine, indicating its multiple potential phosphorylation sites; (3) cC5H11ORF96 is widely expressed in various chicken tissues, with high expression levels in the parathyroid gland, adrenal gland, and pituitary; (4) glucocorticoids (GCs) and stress significantly upregulate C5H11ORF96 mRNA and protein expression in the chicken pituitary and hypothalamus, suggesting its involvement in regulating stress response by influencing the negative feedback of GCs on the HPA axis in chickens. …”
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Ironing out differences in attenuation and blooming artifact in acute stroke thrombi by Aglae Velasco Gonzalez, Dennis Görlich, Boris Buerke, Cristina Sauerland, Norbert Meier, Manfred Fobker, Ray McCarthy, Astrid Jeibmann, Walter Heindel, Andreas Faldum, Harald Kugel

“…Non-contrast CT and a susceptibility-weighted MRI sequence were used for imaging. The study found that RBC content correlated more accurately than iron content with clot attenuation on CT. …”
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Two novel entomopathogenic fungal species of Lecanicillium isolated from soil in China by Tongyi Liu, Wei Chen, Yanhong Tang, Fen Liu, Liyan Yao, Qiongbo Hu, Ke Zhang, Qunfang Weng

“…They differ from known closely related species primarily in sporulation structure, conidia and colony morphological characteristics, and sequence variations in the LSU, SSU, ITS, and TEF gene regions of the ribosomal DNA. …”
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Identification of the Bovine <i>CSN3</i> Core Promoter Region and the Relationships Between <i>CSN3</i> Promoter Polymorphisms and the <i>CSN3</i> A and B Alleles by Wenqing Li, Xiaoyang Wang, Xiuyang Xu, Pinhui Wu, Tong Fu, Liyang Zhang, Tengyun Gao

“…A 2092 bp promoter region of <i>CSN3</i> was re-sequenced for detection, and nine variants were found, of which only three variants had mutation frequencies > 40%, which were −1002T>−, −1654T>A, and −2039T>G. …”
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Modern approach to infusion therapy of pregnant women with gestational hypertension by O.V. Vaskiv, A.P. Grigorenko, O.G. Gorbatyuk, A.S. Shatkovska, A.M. Binkovska, I.A. Priymak

“…The infusion was carried out once a day in the specified sequence for 10 days. The second group included 20 patients with gestational hypertension on basic therapy with methuldopa only. …”
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Transitive reasoning in the adult domestic hen in a six-term series task by R. Degrande, O. Amichaud, B. Piégu, F. Cornilleau, P. Jardat, V. H.B. Ferreira, V. Colson, L. Lansade, L. Calandreau

“…Six hens were trained to learn direct relationships in a sequence of six arbitrary items (A > B > C > D > E > F) in a hybrid training procedure. …”
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LINE-1 methylation status and survival outcomes in colorectal cancer patients: A systematic review and meta-analysis by Akbar Bagheri, Tohid Jafari-Koshki, Leila Alizadeh, Mortaza Raeisi, Yaghoub Moaddab, Abbas Karimi

“…DNA hypomethylation, particularly of Long Interspersed Nuclear Element-1 (LINE-1) repetitive sequences, has been implicated in CRC development and progression. …”
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Influence of multiple décollement and cover rock rheology on the structural evolution of thin-skinned fold-and-thrust belts: insights from discrete element modelling by Eslamirezaei, Nazanin, Alavi, Seyed Ahmad, Nabavi, Seyed Tohid, Ghassemi, Mohammad Reza

“…The present study considers six ternary series of numerical discrete element (DE) models to simulate and explore the effects of mechanical stratigraphy with varied décollement layers, the number of cover sequences and thickness in the structural style, evolution, and strain partitioning of thin-skinned fold-and-thrust belts. …”
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Identification of the AHP family reveals their critical response to cytokinin regulation during adventitious root formation in apple rootstock by Ke Li, Ke Li, Ke Li, Huan Li, Wei Ling Liang, Jing Ju Liu, Hui Yue Tian, Li Hu Wang, Li Hu Wang, Yan Hong Wei, Yan Hong Wei

“…Excluding MdAHP6, other members’ promoter sequences behaved 2-4 CTK response elements. Additionally, the expression characteristics of MdAHPs family members at key stages of AR formation and in different tissues were also examined with exogenous 6-BA and Lov treatments. …”
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NF-κB-Inducing Kinase Is Essential for Effective c-Rel Transactivation and Binding to the <i>Il12b</i> Promoter in Macrophages by Natalia Cuesta, Anna D. Staniszewska, Cristóbal Moreno, Carmen Punzón, Manuel Fresno

“…When looking more specifically at the regulation of the <i>Il12b</i> promoter, we found that c-Rel bound to the NF-kB consensus sequence in macrophages from WT mice 1 hr. after LPS challenge, whereas in <i>aly</i>/<i>aly</i> macrophages, the transcription factor bound to the promoter was p65. …”
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Transcription factor FOXD1 and miRNA-204-5p play a major role in B4GALNT2 downregulation in colon cancer by Martina Duca, Nadia Malagolini, Michela Pucci, Virginie Cogez, Anne Harduin-Lepers, Fabio Dall’Olio

“…Deletion experiments of the putative FOXD1 binding sites in the ~ 2800 bp sequence upstream of the B4GALNT2 transcriptional start site cloned in frame with the luciferase reporter gene, confirmed the regulatory role of FOXD1. …”
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