Genome-wide development of simple sequence repeat (SSR) markers at 2-Mb intervals in lotus (Nelumbo Adans.) by Fengluan Liu, Lin Xi, Naifeng Fu

“…Abstract Background Despite the rapid advancement of high-throughput sequencing, simple sequence repeats (SSRs) remain indispensable molecular markers for various applied and research tasks owing to their cost-effectiveness and ease of use. …”
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Adaptive anti-jamming technologies based on direct sequence spread spectrum and single carrier frequency domain equalization by Fanglin GU, Yuzhen HUANG, Ying ZHAO, Haitao ZHAO, Jibo WEI

Subjects: “…direct sequence spread spectrum…”
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Single-cell RNA sequencing highlights the role of distinct natural killer subsets in sporadic amyotrophic lateral sclerosis by Esther Álvarez-Sánchez, Álvaro Carbayo, Natalia Valle-Tamayo, Laia Muñoz, Joaquim Aumatell, Soraya Torres, Sara Rubio-Guerra, Jesús García-Castro, Judit Selma-González, Daniel Alcolea, Janina Turon-Sans, Alberto Lleó, Ignacio Illán-Gala, Juan Fortea, Ricard Rojas-García, Oriol Dols-Icardo

“…However, no study has investigated the role of peripheral blood immune cells in ALS pathophysiology using single-cell RNA sequencing (scRNAseq). Methods We aimed to characterize immune cells from blood and identify ALS-related immune alterations at single-cell resolution. …”
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Whole-genome sequencing and metagenomics reveal diversity and prevalence of Listeria spp. from soil in the Nantahala National Forest by Jia Wang, Claire N. Schamp, Lauren K. Hudson, Harleen K. Chaggar, Daniel W. Bryan, Katie N. Garman, Mark Radosevich, Thomas G. Denes

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Whole-genome sequencing surveillance of Siberian tick-borne encephalitis virus (TBEV) identifies an additional lineage in Kyrgyzstan by Jake D'Addiego, Mollie Curran-French, Jack Smith, Asankadyr T Junushov, Irena Breininger, Barry Atkinson, John Hay, Roger Hewson

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Single-Cell RNA Sequencing Reveals Macrophage Dynamics During MASH in <i>Leptin</i>-Deficient Rats by Xiaoming Xin, Yaohua Ni, Jing Wang, Fenglin Wu, Meichen Liu, Lingjuan Wu, Jiaxing Dai, Chenglin Wu, Xiaolei Song, Wang Zhang, Guangrui Yang, Ruling Shen, Xianmin Zhu

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Identification and Phylogenetic Tree Determination of Spodoptera frugiperda from Java Based on mtDNA COI Sequence Analysis by Ani Widarti, Enie Tauruslina, Idah Faridah, Willing Bagariang, Hadi Suyanto, Didah Mahmudah, Rista Susanti, Rosalia Maryana, Carwika Carwika

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Retrospective analysis of 300 microbial cell-free DNA sequencing results in routine blood stream infection diagnostics by Claudio Neidhöfer, Claudio Neidhöfer, Niklas Klein, Niklas Klein, Aylin Yürüktümen, Tessa Hattenhauer, Rebekka Mispelbaum, Christian Bode, Tobias A. W. Holderried, Achim Hoerauf, Marijo Parčina

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Infidelity of SARS-CoV Nsp14-exonuclease mutant virus replication is revealed by complete genome sequencing. by Lance D Eckerle, Michelle M Becker, Rebecca A Halpin, Kelvin Li, Eli Venter, Xiaotao Lu, Sana Scherbakova, Rachel L Graham, Ralph S Baric, Timothy B Stockwell, David J Spiro, Mark R Denison

“…Analysis of complete genome sequences from SARS-ExoN mutant viral clones revealed unique mutation sets in every genome examined from the same round of replication and a total of 100 unique mutations across the genome. …”
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Identification of Tree-Related High-Impedance Earth Faults Based on Long-Term Fluctuations in Zero-Sequence Current by Pengwei Wang, Bingyin Xu, Tony Yip, Dong Liang, Guofeng Zou

“…To address the challenge of accurately identifying tree-related high-impedance earth faults (THIEFs), a method based on long-term fluctuations in zero-sequence current is proposed. The analysis of the recorded data from staged tests in a real test network reveals the development patterns of THIEFs and their zero-sequence characteristics during the fault process. …”
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T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses by Billaud, Maud, Theodorou, Ilias, Lamy-Besnier, Quentin, Shah, Shiraz A., Lecointe, François, De Sordi, Luisa, De Paepe, Marianne, Petit, Marie-Agnès

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Protein-Protein Interactions Inferred from Domain-Domain Interactions in Genogroup II Genotype 4 Norovirus Sequences by Chuan-Ching Huang, Chuan Yi Tang

“…However, no efficient vaccine for NoVs exists because of their variable genome sequences. This study investigates the infection processes in protein-protein interactions between hosts and NoVs. …”
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Investigating intra‐tumoural heterogeneity and microenvironment diversity in primary cardiac angiosarcoma through single‐cell RNA sequencing by Jingyuan Huo, Zhen Wang, Wenting Zhao, Miao Chen, Haoyang Li, Fengpu He, Xiao Tian, Yaqi Ma, Firyuza Husanova, Liang Ma, Yiming Ni, Hongda Ding, Weidong Li, Hongfei Xu

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Complex bacterial diversity of Guaymas Basin hydrothermal sediments revealed by synthetic long-read sequencing (LoopSeq) by John E. Hinkle, John E. Hinkle, Jeffrey P. Chanton, Molly A. Moynihan, Molly A. Moynihan, S. Emil Ruff, S. Emil Ruff, Andreas Teske

“…Previous studies of microbial community structure in hydrothermal sediments have typically used short-read sequencing approaches. To improve on these approaches, we use LoopSeq, a high-throughput synthetic long-read sequencing method that has yielded promising results in analyses of microbial ecosystems, such as the human gut microbiome. …”
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Native RNA nanopore sequencing reveals antibiotic-induced loss of rRNA modifications in the A- and P-sites by Anna Delgado-Tejedor, Rebeca Medina, Oguzhan Begik, Luca Cozzuto, Judith López, Sandra Blanco, Julia Ponomarenko, Eva Maria Novoa

“…Here, we systematically characterize bacterial rRNA modifications upon exposure to diverse antibiotics using native RNA nanopore sequencing. To identify significant rRNA modification changes, we develop NanoConsensus, a novel pipeline that is robust across RNA modification types, stoichiometries and coverage, with very low false positive rates, outperforming all individual algorithms tested. …”
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Blind estimation of pseudo-random codes in non-periodic long code direct-sequence spread-spectrum signals by HAOZhi-jin Z, UXiao-wei G, HENLei S, HANYi Z

Subjects: “…non-periodic long code direct-sequence spread-spectrum (NPLC-DSSS) signal…”
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Transposon-sequencing across multiple Mycobacterium abscessus isolates reveals significant functional genomic diversity among strains by Chidiebere Akusobi, Sanjeevani Choudhery, Bouchra S. Benghomari, Ian D. Wolf, Shreya Singhvi, Thomas R. Ioerger, Eric J. Rubin

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Exploring Core Genes Associated with Sepsis and Systemic Inflammatory Response Syndrome Using Single-Cell Sequencing Technology by Shen Y, Leng L, Hu Y

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Taxonomy and DNA Sequencing of Crematogaster coriaria Mayr, 1872 (Hymenoptera: Formicidae), with Redescriptions of the Worker, Queen, and Male Castes by Shingo Hosoishi, Kazuo Ogata

“…The polymorphism of C. coriaria Mayr, 1872, was also discussed. DNA sequences were generated for C. coriaria Mayr, 1872, using an amplification of second half region of the mitochondrial gene COI with a total of 711 bp. …”
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Comparison of Illumina and Oxford Nanopore sequencing data quality for Clostridioides difficile genome analysis and their application for epidemiological surveillance by Semeh Bejaoui, Sofie Holtsmark Nielsen, Astrid Rasmussen, John Eugenio Coia, Dorte Terp Andersen, Tobias Bruun Pedersen, Martin Vad Møller, Marc Trunjer Kusk Nielsen, Dorte Frees, Søren Persson

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