<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci by E. A. Anisimova, N. A. Fakhrutdinov, D. A. Mirgazov, E. A. Dodonova, I. A. Elizarova, M. E. Gorbunova, N. I. Khammadov, L. I. Zainullin, K. A. Osyanin

“…Modern methods of molecular biology make it possible to detect a number of genetic markers suitable for indicating and differentiating the strains of B. anthracis, including the loci arranged as variable number tandem repeats (VNTRs) and SNPs, one nucleotide-sized differences in the DNA sequence of the loci being compared. The objective of the present study was to examine the effectiveness of SNP analysis and PCR amplif ication of VNTR loci combined with the high-resolution amplicon melting analysis for identif ication and differentiation of the anthrax agent strains. …”
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Recombinant factor VIIa: new insights into the mechanism of action through product innovation by Miguel A. Escobar, Maureane Hoffman, Giancarlo Castaman, Cedric Hermans, Johnny Mahlangu, Johannes Oldenburg, Charles L. Percy, Mark T. Reding, Amy D. Shapiro, Steven W. Pipe

“…Eptacog alfa and eptacog beta share the same amino acid sequence but differ in posttranslational modifications. …”
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Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea by Tina Bonačić, Jasna Arapov, Ivana Bušelić, Ivana Lepen Pleić, Blanka Milić Roje, Tina Tomašević, Mia Bužančić, Marija Mladinić, Silvia Casabianca, Antonella Penna, Sanda Skejić, Živana Ninčević Gladan

“…Our findings underscore the value of a combined approach for reliable species identification and contribute to the development of genetic sequence databases that support the advancement of next-generation methods such as metabarcoding. …”
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Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases in Escherichia coli by Ayesha Siddiqui, Muhammad Mudassar Iqbal, Asad Ali, Iqra Fatima, Hazrat Ali, Aamir Shehzad, Sameer H. Qari, Ghulam Raza, Muhammad Aamer Mehmood, Peter J. Nixon, Niaz Ahmad

“…The codon-optimized gene sequences were synthesized and placed under a tobacco chloroplast 16S rRNA promoter (Prrn), along with the 5′ UTR (untranslated region) from gene 10 of phage T7 (T7g10). …”
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Effect of ON and OFF Axis Open Hole Tensile Testing of GFRP/Epoxy Composites by Sunith Babu Loganathan, Ashok Kumar Krishnappa, Jaya Christiyan Kumaravelu Grace Jesu Bai, Ritin Pavirala, Rajesh Mathivanan

“…The effect of loading under different sequences was studied. The nature of failure near the hole region was reviewed and presented. …”
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Template switching during DNA replication is a prevalent source of adaptive gene amplification by Julie N Chuong, Nadav Ben Nun, Ina Suresh, Julia Cano Matthews, Titir De, Grace Avecilla, Farah Abdul-Rahman, Nathan Brandt, Yoav Ram, David Gresham

“…The gene is flanked by two long terminal repeats (LTRs) and proximate to an origin of DNA replication (autonomously replicating sequence, ARS), which likely promote rapid GAP1 CNV formation. …”
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Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development by Xufeng Guo, Na He, Biying Huang, Chongyao Chen, Yanxia Zhang, Xiaoyu Yang, Jie Li, Zhigang Dong

“…Consequently, we conducted a comprehensive bioinformatics analysis of all <i>AAAP</i> genes in grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship analysis, and gene expression profiling. …”
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An Investigation of Ionospheric TEC Prediction Maps Over China Using Bidirectional Long Short‐Term Memory Method by Shuangshuang Shi, Kefei Zhang, Suqin Wu, Jiaqi Shi, Andong Hu, Huajing Wu, Yu Li

“…The bi‐LSTM‐based model shows the best performance, which is most likely due to the fact that the sequence information in both forward and backward directions is taken into consideration in the new model. …”
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Once a Triple, Not Always a Triple: The Evolution of Hierarchical Triples That Yield Merged Inner Binaries by Cheyanne Shariat, Smadar Naoz, Kareem El-Badry, Antonio C. Rodriguez, Bradley M. S. Hansen, Isabel Angelo, Alexander P. Stephan

“…More than half of all main-sequence (MS) stars have one or more companions, and many of those with initial masses <8 M _⊙ are born in hierarchical triples. …”
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Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China by Yidan Wang, Mengjun Jin, Chengde Yang, Fengfeng Cai, Richard Osei, Ting Ma, Cuiwen Zhang, Na Qi

“…Meanwhile, its internal transcribed spacer sequence (ITS) and DNA gyrase A subunit (gyrA) were both more than 99% homologous and clustered on the same branch with B. subtilis. …”
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Production of ACE inhibitory peptides via ultrasonic-assisted enzymatic hydrolysis of microalgal Chlorella protein: Process improvement, fractionation, identification, and in silic... by Jeeraporn Pekkoh, Apiwit Kamngoen, Antira Wichaphian, May Thu Zin, Supakit Chaipoot, Kamon Yakul, Wasu Pathom-aree, Wageeporn Maneechote, Benjamas Cheirsilp, Kuan Shiong Khoo, Sirasit Srinuanpan

“…Q-TOF-LC-MS/MS analysis of the LMW peptides verified the appearance of active sequences, identifying up to 49 LMW fragments within the fractionated LMW peptides. …”
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Alterations in fecal bacteriome virome interplay and microbiota-derived dysfunction in patients with schizophrenia by Shiwan Tao, Yulu Wu, Liling Xiao, Yunqi Huang, Han Wang, Yiguo Tang, Siyi Liu, Yunjia Liu, Qianshu Ma, Yubing Yin, Minhan Dai, Min Xie, Jia Cai, Zhengyang Zhao, Qiuyue Lv, Jiashuo Zhang, Mengting Zhang, Menghan Wei, Yang Chen, Mingli Li, Qiang Wang

“…However, little is known about the role of the gut virome on shaping the gut bacteriome in SCZ. Here in, we sequenced the fecal virome, bacteriome, and host peripheral metabolome in 49 SCZ patients and 49 health controls (HCs). …”
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Does the transfer of knowledge from the pioneer generation to the second‐generation speed‐up the learning curve of robot‐assisted partial nephrectomies? TRANSFER trial (UroCCR n°83... by Louis Vignot, Zine‐Eddine Khene, Adil Mellouki, Arnoult Morrone, Jean‐Christophe Bernhard, Karim Bensalah, Daniel Chevallier, Nicolas Doumerc, Morgan Roupret, Francois‐Xavier Nouhaud, Cédric Lebacle, Jean‐Alexandre Long, Pierre Pillot, Xavier Tillou, Brannwel Tibi, Matthieu Durand, Younes Ahallal, Imad Bentellis

“…There was a univariate analysis on three consecutive sequences of 15 procedures. The learning speed for WIT was explored graphically by polynomial regression after cubic splines. …”
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Geochemical and mineralogical differences between supergene and hypogene gold mineralization and influence on gold recovery: A case study of the Faina gold deposit, Pitangui greens... by Gabriel Machado da Silva, Atlas Vasconcelos Corrêa Neto, Mariana Brando Soares, Felipe Emerson André Alves, Nicollas de Oliveira Ferreira Santos, Piero Azevedo Berquó de Sampaio, Vitor Diniz Silveira, Gabriel Faria Gonçalves

“…The stratigraphy at Faina consists of a sequence of meta-mafic and meta-sedimentary rocks, with a thick (>40m) saprolite horizon (oxidized portion). …”
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Experimental and numerical analysis of Carbon/Glass fiber reinforced thermoplastic wind turbine blade prepared by VARIM by H N Abhilash, K Raghunandana, Subraya Krishna Bhat, Aruna Prabhu, A Amar Murthy

“…The wind turbine blade (WTB) is simulated in ANSYS ACP based on the designated stacking sequence and fiber orientation. Static structure analysis is performed for the thermoplastic and thermosetting composites under various operating circumstances. …”
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Simultaneous Inference of Past Demography and Selection from the Ancestral Recombination Graph under the Beta Coalescent by Korfmann, Kevin, Sellinger, Thibaut Paul Patrick, Freund, Fabian, Fumagalli, Matteo, Tellier, Aurélien

“…We tackle these limitations by developing two novel and different approaches to infer multiple merger events from sequence data or the ancestral recombination graph (ARG): a sequentially Markovian coalescent (SMβC) and a graph neural network (GNNcoal). …”
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Reclassification of Pterulaceae Corner (Basidiomycota: Agaricales) introducing the ant-associated genus Myrmecopterula gen. nov., Phaeopterula Henn. and the corticioid Radulomyceta... by Caio A. Leal-Dutra, Gareth W. Griffith, Maria Alice Neves, David J. McLaughlin, Esther G. McLaughlin, Lina A. Clasen, Bryn T. M. Dentinger

“…In this study, we generated 278 sequences from both newly collected and fungarium samples. …”
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In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI by Carolin Horstmeier, Annette B. Ahrberg, Dagmar Berner, Janina Burk, Claudia Gittel, Aline Hillmann, Julia Offhaus, Walter Brehm

“…MRI included standard and magic angle image series in T1- and T2∗-weighted sequences performed at regular intervals. Image analysis comprised blinded evaluation and quantitative assessment of signal-to-noise ratio. …”
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Reduction of monoclonal antibody viscosity using interpretable machine learning by Emily K. Makowski, Hsin-Ting Chen, Tiexin Wang, Lina Wu, Jie Huang, Marissa Mock, Patrick Underhill, Emma Pelegri-O’Day, Erick Maglalang, Dwight Winters, Peter M. Tessier

“…Here, we report an interpretable machine learning model for predicting antibody (IgG1) variants with low viscosity using only the sequences of their variable (Fv) regions. Our model was trained on antibody viscosity data (>100 mg/mL mAb concentration) obtained at a common formulation pH (pH 5.2), and it identifies three key Fv features of antibodies linked to viscosity, namely their isoelectric points, hydrophobic patch sizes, and numbers of negatively charged patches. …”
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Molecular Detection of <i>Rickettsia</i> spp. and Other Tick-Borne Pathogens in Ticks from a Nature Reserve: Implications for Zoonotic Transmission by Santina Di Bella, Valeria Blanda, Silvia Scibetta, Ilenia Giacchino, Antonino Gentile, Giuseppina Chiarenza, Vincenza Cannella, Giovanni Provinzano, Francesca Grippi, Annalisa Guercio

“…Phylogenetic analyses were conducted on <i>Rickettsia</i> sequences. The range of ticks and TBPs present in this area highlights potential public health concerns. …”
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