Preliminary Evidence of Human Plasmodium in Domestic Animals from a Malaria-Endemic Region in Indonesia [version 4; peer review: 2 approved, 1 approved with reservations, 1 not app... by Munirah Munirah, Sitti Wahyuni, Firdaus Hamid, Isra Wahid

“…Nested PCR was used for parasite molecular detection, while Plasmodium species were identified using the sequenced DNA. Results A total of 208 and 62 animal blood samples were collected from Gaura village, West Sumba and Fakfak village, West Papua, Indonesia respectively. …”
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Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis by Xueqin Huang, Yingqi Yang, Hanlin Zhou, Liping Hu, Annan Yang, Hua Jin, Biying Zheng, Jiang Pi, Jun Xu, Pinghua Sun, Huai-Hong Cai, Xujing Liang, Bin Pan, Junxia Zheng, Haibo Zhou

“…In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. …”
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Genetic Variability of Aspergillus flavus Isolates from a Mississippi Corn Field by Cesar D. Solorzano, Hamed K. Abbas, Robert M. Zablotowicz, Perng-Kuang Chang, Walker A. Jones

“…Of the 175 A. flavus isolates examined, 74 and 97 had the typical norB-cypA type I (1.5 kb) and type II (1.0 kb) deletion patterns, respectively. Variability in the sequence of the omtA gene of the majority of the field isolates (n=118) was compared to strain K49. …”
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Novel introductions of human-origin H3N2 influenza viruses in swine, Chile by Belen Monserrat Agüero, Naomi Ariyama, Felipe Berrios, Nikita Enciso, Barbara Quezada, Rafael A. Medina, Rafael A. Medina, Victor Neira, Victor Neira

“…Observing global IAV sequences, a similar pattern was identified in the USA confirming the reverse zoonotic potential of current seasonal human H3N2 strains. …”
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First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China by Chen Chen, Jin Tao, Ling Tang, Tong Sun, Zhuyun Sun, Huan Xu, Shuaitao Deng, Cheng Xue, Xing Ji, Rui Wang, Hongjun Chen

“…Methods The wild raccoon samples were collected from the residential area of Shanghai and screened for parvovirus, followed by virus isolation and sequence comparison analysis. Results In this study, three strains of parvovirus, YSH1‐2022, YSH2‐2022 and GSW1‐2023, were isolated from anal and pharyngeal swab mixtures of 42 live raccoon dogs and two dead raccoon dogs in Shanghai, China. …”
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Diversity and Multigene Phylogeny of the Genus <i>Floccularia</i> (Agaricales, Basidiomycota) by Zai-Wei Ge, Hua Qu, Malka Saba, Tian Gao, Martin Ryberg

“…Based on worldwide sampling and multi-locus DNA sequence data (ITS, LSU, <i>rpb2</i>, <i>tef1</i>), the phylogeny of <i>Floccularia</i> was reconstructed, and the species diversity in Asia was critically studied on the basis of morphology and phylogeny. …”
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Anther transcriptomes in cold-tolerant rice cultivars tend to show insensitive responses by Koichi Yamamori, Seiya Ishiguro, Kei Ogasawara, Kayyis Muayadah Lubba, Kaien Fujino, Kazumitsu Onishi, Yutaka Sato, Yuji Kishima

“…Minimal changes in genes and repetitive sequences under cold stress also tended to be characteristic of the cold-tolerant lines. …”
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Canadian Public Health Laboratory Network laboratory Guidelines for the Use of Serological Tests (excluding point-of-care tests) for the Diagnosis of Syphilis in Canada by Paul N Levett, Kevin Fonseca, Raymond SW Tsang, Kamran Kadkhoda, Bouchra Serhir, Sandra M Radons, Muhammad Morshed

“…Both traditional and reverse sequence algorithms are recommended and the algorithm used should be based on a combination of local disease epidemiology, test volumes, performance of the proposed assays and available resources.…”
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Feature selection enhances peptide binding predictions for TCR-specific interactions by Hamid Teimouri, Hamid Teimouri, Zahra S. Ghoreyshi, Zahra S. Ghoreyshi, Anatoly B. Kolomeisky, Anatoly B. Kolomeisky, Anatoly B. Kolomeisky, Jason T. George, Jason T. George, Jason T. George, Jason T. George

“…The results of our feature selection method are consistent with findings from hybrid approaches that utilize both sequence and structural data as input as well as experimental data.DiscussionOur theoretical approach highlights the role of feature selection in peptide-TCR interactions, providing a quantitative tool for uncovering the molecular mechanisms of the T-cell response and assisting in the design of more advanced targeted therapeutics.…”
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P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors by Marjory Alana Marcello, Elaine Cristina Morari, Lucas Leite Cunha, Aline Carolina De Nadai Silva, Dirce Maria Carraro, André Lopes Carvalho, Fernando Augusto Soares, José Vassallo, Laura Sterian Ward

“…ACIS-III system was used to evaluate p53 and immune cell markers including tumor-associated macrophages (TAM); CD68 and tumor-infiltrating lymphocytes (TIL) subsets such as CD3, CD4, CD8, and CD20 in 206 thyroid carcinomas, 105 benign nodules, and 18 normal tissues. Also, TP53 was sequenced in 78 out of 164 patients with papillary thyroid carcinoma. …”
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Epidemiological survey on tick borne diseases of pet dogs in korla, northwestern china by Jianhui CHEN, Shen SHI 2, Nannan CUI, Lixin SU, Ziqi WANG, Fang LI, Shang ZHAN, Guoyu ZHAO, Yuanzhi WANG, Xijiang WANG

“…We used primers targeting the 16S ribosomal (16S rRNA) gene for detection of Anaplasma bovis species, targeting the small subunit 18S ribosomal RNA(18S rRNA) gene for detection of Hepatozoon canis species and targeting htpAB-associated repetitive element gene (IS111) for detection of Coxiella burnetii species The nested PCR(nPCR)-positive products were sequenced, aligned, and phylogenetically analyzed. three tick-borne pathogens were detected in the samples. …”
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Through-wall Human Pose Reconstruction and Action Recognition Using Four-dimensional Imaging Radar by Rui ZHANG, Hanqin GONG, Ruiyuan SONG, Yadong LI, Zhi LU, Dongheng ZHANG, Yang HU, Yan CHEN

“…By preserving 3D spatial information and using long-sequence temporal information, the proposed ST2W-AP network considerably enhances the pose estimation and behavior recognition performance. …”
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A Generalized Phase-Shift PWM Extension for Improved Natural and Active Balancing of Flying Capacitor Multilevel Inverters by Georgios Kampitsis, Efstratios I. Batzelis, Antonis Kolokasis, Elison Matioli, Bikash C. Pal

“…We provide an easy and intuitive method for the extraction of the PWM pattern, the switching states, and their sequence. Simulations were carried out in Matlab&#x002F;Simulink and experimental tests were conducted on a single-phase 7-level GaN inverter prototype. …”
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Restriction Enzyme Analysis of DNA Methylation in “Condensed” Chromatin of Ha-Ras-Transformed NIH 3T3 Cells by Maria Luiza S. Mello, Ann F. Chambers, Benedicto C. Vidal, Wolfgang Planding, Ulrich Schenck

“…The amount of methylated CpG sequences not digested by Hpa II in “condensed” chromatin regions was found to vary in the studied ras‐transformed cell lines. …”
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Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles by Luzhao Pan, Jiaqiu Zheng, Jia Liu, Jun Guo, Fawan Liu, Lecheng Liu, Hongjian Wan

“…In this study, based on the whole genome sequence, we performed a systematic analysis for the ASMT gene family in pepper (Capsicum annuum L.) and analyzed their expression profiles during growth and development, as well as abiotic stresses. …”
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Entropy-extreme concept of data gaps filling in a small-sized collection by Viacheslav Kovtun, Krzysztof Grochla, Mohammed Al-Maitah, Saad Aldosary, Oleksii Kozachko

“…In the case of addressing missing values in input data, these are interpreted as results of transforming a sequence of independent stochastic vectors introduced into a model structurally identical to that formalized for filling gaps in output data. …”
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Quantifying preferential flow occurrence in dependence of land cover on the southern slopes of Mount Kilimanjaro, Tanzania by Frank Paul Shagega, Fabia Codalli, Suzanne Jacobs, Subira Eva Munishi, David Windhorst, Lutz Breuer

“…After a separation and clustering of rainfall events we assessed the dynamics of soil moisture responses. The sequence and timing of soil moisture changes informed about the occurrence of preferential versus sequential (matrix) flow. …”
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PcWRKY1 Represses Transcription of Yellow Stripe‐Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems by Xin Chen, Yuhong Zhang, Yao Cheng, Wenjian Yu, Lingyu Yang, Peiqi Shu, Jing Zhou, Payam Fayyaz, Zhi‐Bin Luo, Shurong Deng, Wenguang Shi

“…PcWRKY1 can bind to the PcYSL3 promoter sequence and represses its expression. PcWRKY1 inhibits Cd accumulation in the wood and bark of plants. …”
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Ulcerative Colitis and Its Association with Salmonella Species by Manish Kumar Tripathi, Chandra Bhan Pratap, Vinod K. Dixit, Tej Bali Singh, Sunit K. Shukla, Ashok K. Jain, Gopal Nath

“…While 6.8% of UC, 10.7% of CC, 11.0% of IBS, and 12.1% of healthy subjects were positive for the antibody, the PCR positivity rates for Salmonella specific sequences were 79.7% in UC, 53.6% in CC, 66.1% in IBS, and 16.3% in healthy controls. …”
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MicroRNA Expression Profiling in Behçet’s Disease by Antonio Puccetti, Andrea Pelosi, Piera Filomena Fiore, Giuseppe Patuzzo, Claudio Lunardi, Marzia Dolcino

“…Methods. miRNA microarray analysis was performed in blood cells of BD patients and healthy controls. miRNA expression profiles were analyzed using Affymetrix arrays with a comprehensive coverage of miRNA sequences. Pathway analyses were performed, and the global miRNA profiling was combined with transcriptoma data in BD. …”
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