Single-cell and bulk RNA sequencing analysis reveals CENPA as a potential biomarker and therapeutic target in cancers by Hengrui Liu, Miray Karsidag, Kunwer Chhatwal, Panpan Wang, Tao Tang

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Genome sequencing and comparison of five Tilletia species to identify candidate genes for the detection of regulated species infecting wheat by Hai D. T. Nguyen, Tahera Sultana, Prasad Kesanakurti, Sarah Hambleton

“…Of the DNA-based methods published so far, most have focused on sequence variation among tested strains at a single gene locus. …”
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Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria by Wonganu Benjamaporn, Paenthong Mathaphon, Roytrakul Sittiruk

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Arctiin Protects Chondrocytes from Interleukin-1β-Induced Inflammation and Apoptosis by RNA Sequence In vivo and In vitro by Yong-Jia Song, Jia-Min Bao, Zeng-Qiao Zhang, Yun-Xiang Hai, Hao-Nan Wen, Tian-Jun Zhai, Wei Feng, Min Song

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Blind detection for image steganography using short du ate codes statistical model for Hilbert scanning sequences by Shang-ping ZHONG, Qiao-fen XU, Wen-zhong GUO, Bin LIAO

“…By analyzing and proving the correlation between the detection capability of a short duplicate code statistical feature and the probability of cumulating short duplicate codes、the dimension of short duplicate codes,a method to improving the detection capability of a short duplicate code statistical feature was found.Then,a blind detection method for image steganography using short duplicate codes statistical model for Hilbert scanning sequences was proposed.The proposed method used Poisson distribution test to detect the stego-message based on the statistical feature of short duplicate code with same elements in LSB Hilbert scanning sequences.So,the proposed method could make full use of Hilbert curve to maintain the good properties of local correlation,and could not only use the correlation of adjacent elements,but also use the correlation of elements in local regions.Theoretical lysis and experiments show that the proposed method can effectively improve the detection rate under the condition of effectively controlling the false alarm rate.…”
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Effect of Transient Nicotine Load Shock on the Performance of Pseudomonas sp. HF-1 Bioaugmented Sequencing Batch Reactors by Dong-sheng Shen, Li-jia Wang, Hong-zhen He, Mei-zhen Wang

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Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B by John A. Lednicky, Thomas B. Waltzek, Micah D. Halpern, Sara B. Hamilton

“…The hPIV-4B genomic sequence that we determined conforms to the paramyxovirus “rule of six.” …”
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Whole genome sequence of Streptomyces colonosanans strain MUSC 93JT isolated from mangrove forest in Malaysia by Hooi-Leng Ser, Jodi Woan-Fei Law, Wen-Si Tan, Wai-Fong Yin, Kok-Gan Chan

“…Due to these data, the strain was subjected to whole genome sequencing to uncover its genomic potential and further improve the understanding of the strain. …”
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High-throughput sequence-based microsatellite genotyping for the non-model Neotropical tree species Anadenanthera colubrina (Leguminosae) by Alejandra Lorena Goncalves, María Victoria García, Emilie Chancerel, Olivier Lepais, Myriam Heuertz

“…Previously available genetic markers for A. colubrina consisted of a few microsatellites. Next-generation sequencing (NGS) strategies allow simple and cost-effective development of new SSR loci from low-coverage whole genome shotgun sequencing. …”
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Influence of welding sequences on residual stress and deformation of U-rib components fabricated by laser-arc hybrid welding by Shaoning Geng, Yuantai Li, Ping Jiang, Yilin Wang, Jun Jin, Chu Han

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Séquence ERC : comment améliorer l’utilisation des méthodes de dimensionnement de la compensation écologique  ? by Agnès Mechin, Sylvain Pioch

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A walk through the development of human leukocyte antigen typing: from serologic techniques to next-generation sequencing by Haeyoun Choi, Eun-Jeong Choi, Hyoung-Jae Kim, In-Cheol Baek, Aegyeong Won, Su Jin Park, Tai-Gyu Kim, Yeun-Jun Chung

Subjects: “…human leukocyte antigen; histocompatibility testing; transplantation; next-generation sequencing…”
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Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis by Jayram Karmacharya, Prasansah Shrestha, So-Ra Han, Hyun Park, Tae-Jin Oh

“…We identified a species of Arthrobacter isolated from seawater in the polar region using 16S rRNA sequence analysis. The strain PAMC25284 genome consists of a circular chromosome with a GC content of 65.6% and is projected to contain 3,588 genes, of which 3,150 are protein coding, 366 are pseudogenes, 19 are rRNA coding, and 50 are tRNA coding genes. …”
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Florfenicol Resistance in Enterobacteriaceae and Whole-Genome Sequence Analysis of Florfenicol-Resistant Leclercia adecarboxylata Strain R25 by Yuanyuan Ying, Fei Wu, Chongyang Wu, Yi Jiang, Min Yin, Wangxiao Zhou, Xinyi Zhu, Cong Cheng, Licheng Zhu, Kewei Li, Junwan Lu, Teng Xu, Qiyu Bao

“…The resistance gene-related mobile genetic elements also share homologous sequences from different species or genera of bacteria. …”
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Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological Advantage by Rob E. Carpenter, Vaibhav Tamrakar, Harendra Chahar, Tyler Vine, Rahul Sharma

“…All 78 samples were classified into WHO lineages by whole-genome sequencing and then compared with two commercially available RT-qPCR assays for spike protein mutation(s). …”
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Microbial diversity and biogeography across gastrointestinal tracts of Takifugu pufferfish revealed by full-length 16S amplicon sequencing by Xingkun Jin, Yan Shi, Zhenlong Sun, Yaohui Wang, Zhe Zhao

Subjects: “…Amplicon sequencing…”
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Detection of inversion with breakpoints in ARSB causing MPS VI by whole-genome sequencing: lessons learned and best practices by Yufeng Huang, Wenyue Deng, Hui Huang, Xiankai Zhang, Xiaohong Chen, Jian Ye, Sukun Luo, Ting Yu, Hui Yao, Hao Du, Xuelian He

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Leveraging the fundamentals of heat transfer and fluid mechanics in microscale geometries for automated next-generation sequencing library preparation by Olivia Ott, Sabrina Tolppi, Jennifer Figueroa-Cruz, Khaliun Myagmar, Khulan Unurbuyan, Anubhav Tripathi

“…Abstract Next-generation sequencing (NGS) is emerging as a powerful tool for molecular diagnostics but remains limited by cumbersome and inefficient sample preparation. …”
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Rapid Identification of Drug-Resistant Tuberculosis Genes Using Direct PCR Amplification and Oxford Nanopore Technology Sequencing by Kaishun Zhao, Chunlin Tu, Wei Chen, Haiying Liang, Wenjing Zhang, Yilei Wang, Ye Jin, Jianrong Hu, Yameng Sun, Jun Xu, Yanfang Yu

“…This study established a sequencing method and bioinformatics pipeline for resistance gene analysis using an Oxford Nanopore Technologies sequencer. …”
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Transcriptome sequencing reveals jasmonate playing a key role in ALA-induced osmotic stress tolerance in strawberry by Yan Zhong, Xin Wei, Jianting Zhang, Liangju Wang

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