Whole Genome Sequencing Reveals Substantial Genetic Structure and Evidence of Local Adaptation in Alaskan Red King Crab by Carl A. St. John, Laura E. Timm, Kristen M. Gruenthal, Wesley A. Larson

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16S rRNA Sequencing and Metabolomics to Analyze Correlation Between Fecal Flora and Metabolites of Squabs and Parent Pigeons by Xiaobin Li, Shengchen Zheng, Haiying Li, Jiajia Liu, Fan Yang, Xiaoyu Zhao, Yafei Liang

“…Fecal samples were collected at 15 days of age, and differences in the microbiota and metabolites between the two groups were analyzed using 16S rRNA sequencing and LC-MS/MS. The results showed the following: (1) Squabs exhibited significantly lower α diversity, with a reduction in their Chao1 index and observed OTUs compared to the parent pigeons. (2) Firmicutes dominated the fecal microbiota in both groups, but parent pigeon feces showed a notably higher abundance of Proteobacteria. …”
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Chromosome 1 Sequence Analysis of C57BL/6J-Chr1KM Mouse Strain by Fuyi Xu, Tianzhu Chao, Yiyin Zhang, Shixian Hu, Yuxun Zhou, Hongyan Xu, Junhua Xiao, Kai Li

“…Both analyses suggested that the Chr 1 sequence of B6-Chr1KM was predominantly derived from M. m. domesticus while 9.7% of the sequence was found to be from M. m. musculus. …”
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Signatures of lower respiratory tract microbiome in children with severe community-acquired pneumonia using shotgun metagenomic sequencing by Ting-Yu Yen, Ching Hsu, Ni-Chung Lee, Chao-Szu Wu, Hsin Wang, Kuan-Yi Lee, Chia-Ray Lin, Chun-Yi Lu, Mo-Li Tsai, Tzu-Yu Liu, Che Lin, Chien-Yu Chen, Luan-Yin Chang, Feipei Lai, Li-Min Huang

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Whole exome sequencing identifies ABHD14A and MRNIP as novel candidate genes for developmental language disorder by Amal Bouzid, Malek Belcadhi, Amal Souissi, Meryam Chelly, Fakher Frikha, Hela Gargouri, Crystel Bonnet, Fida Jebali, Salma Loukil, Christine Petit, Saber Masmoudi, Rifat Hamoudi, Mariem Ben Said

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The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing by Ping Sun, Zhiyong Guo, Daiping Guo, Jian Wang, Tingting Wu, Tingjun Li, Jiannan Liu, Xinhua Liu

“…Then, the V3-V4 hypervariable regions of the 16S rRNA gene were amplified and subjected to next-generation sequencing. The statistical analysis was performed by R software (V3.5.1). …”
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A single-cell RNA sequencing dataset of peripheral blood cells in long COVID patients on herbal therapy by Karolina Hanna Prazanowska, Tae-Hun Kim, Jung Won Kang, Young-Hee Jin, Sunoh Kwon, Su Bin Lim

“…To ensure the technical validity of our dataset, we implemented rigorous quality checks throughout stages of the study, including sample preparation, sequencing, and bioinformatic data analysis levels. …”
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Genome-wide DNA methylation and transcriptome sequencing analyses of lens tissue in an age-related mouse cataract model. by Yuzhu Hu, Dongmei Su, Yue Zhang, Yanjiang Fu, Sijia Li, Xiaoya Chen, Xiao Zhang, Shunfei Zheng, Xu Ma, Shanshan Hu

“…In this study, we used a mouse model and performed DNA methylation and transcriptome sequencing analyses to find epigenetic indicators for age-related cataracts (ARC). …”
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Exome Sequencing: Mutilating Sensory Neuropathy with Spastic Paraplegia due to a Mutation in FAM134B Gene by Salma M. Wakil, Dorota Monies, Samya Hagos, Fahad Al-Ajlan, Josef Finsterer, Aisha Al Qahtani, Khushnooda Ramzan, Rawan Al Humaidy, Mohamed A. Al-Muhaizea, Brian Meyer, Saeed A. Bohlega

“…We employed homozygosity mapping and exome sequencing which is an efficient strategy to characterize the recessive genes, thus obtaining a rapid molecular diagnosis for genetically heterogeneous disorders like HSAN. …”
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Complete Whole-Genome Sequence of Streptomyces sp. MUM 178J, a Potential Anti-Vibrio Agent by Ke-Yan Loo, Loh Teng-Hern Tan, Kah-Ooi Chua, Priyia Pusparajah, Kok-Gan Chan, Learn-Han Lee, Jodi Woan-Fei Law, Vengadesh Letchumanan

“…Therefore, the strain was subjected to whole genome sequencing to unearth its genomic potential. The genome of Streptomyces sp. …”
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Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections by B Alraddadi, S Al-Azri, KR Forward

“…INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.…”
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Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties by O. Y. Antonova, N. S. Klimenko, Z. Z. Evdokimova, L. I. Kostina, T. A. Gavrilenko

“…The genotypes selected through the molecular markers were divided into four groups: (A) 13 PVY resistant varieties carrying diagnostic markers of the Rysto, Ry-fsto genes and having sterile mt-type gamma; (B) four varieties possessing mt-type gamma and not having the markers of the R genes introgressed from S. stoloniferum; (C) eight genotypes carrying five gene-specific markers for the RB/Rpi-blb1/= Rpi-sto1; (D) the rest 166 (86.9 %) varieties not possessing any of the diagnostic markers associated with the S. stoloniferum genetic material. The sequences of the Rpi-sto1- and BLB1 F/R-amplicons were identical in all the genotypes of group ‘C’ and showed respective 99 % and 100 % similarity to the corresponding fragments of the Rpi-sto1 and Rpi-blb1 genes from the GenBank database. …”
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Genomic and epidemiological analysis of SARS-CoV-2 variants isolated in Guinea: a routine sequencing implementation by Aminata Mbaye, Haby Diallo, Thibaut Armel Cherif Gnimadi, Kadio Jean Jacques Olivier Kadio, Abdoul Karim Soumah, Joel Balle Koivogui, Jean Louis Monemou, Moriba Kowa Povogui, Djiba Kaba, Castro Hounmenou, Laetitia Serrano, Christelle Butel, Nicolas Fernandez Nuñez, Nicole Vidal, Emilande Guichet, Eric Delaporte, Ahidjo Ayouba, Martine Peeters, Abdoulaye Toure, Alpha Kabinet Keita, AFROSCREEN Team

“…The Illumina iSeq 100 technology was used. The sequences were then analyzed using the GeVarli pipeline to generate consensus sequences and variant calling. …”
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Progressive multifocal leukoencephalopathy, the role of the susceptibility-weighted imaging sequence in a case of immunosuppression of an initially unknown cause by Giuseppe Maria Andrea D’Arma, Rosario Francesco Balzano, Federica Masino, Giuseppe Guglielmi

Subjects: “…susceptibility-weighted imaging sequence…”
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VARIABILITY OF MITOCHONDRIAL CYTOCHROME OXYDASE SUBUNIT I GENE SEQUENCE IN SPECIES OF THE GENERA AEDES AND OCHLEROTATUS (DIPTERA: CULICIDAE) by N. V. Khrabrova, Yu. V. Andreeva, O. V. Vaulin, S. S. Alekseeva, A. K. Sibataev

“…Sequence variation in the 538-bp fragment from the 5′ end of the COI region was analyzed to test its usefulness in the identification of 15 mosquito species of the genera Aedes and Ochlerotatus (Diptera: Culicidae) from Tomsk and Kemerovo regions and 14 species from GenBank. …”
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The genome sequence of Cory’s shearwater, Calonectris borealis (Cory, 1881) [version 1; peer review: 2 approved] by Joan Ferrer Obiol, Guillem Izquierdo Arànega, Marta Riutort León, Raül Ramos Garcia, Jacob González-Solís Bou, Julio Rozas Liras

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['EVOLUTION OF KARAKORAM SEQUENCE, HUNZA VALLEY NE PAKISTAN: EVIDENCES FROM FIELD MAPPING AND MICRO-ANALYTICAL WORK'] by S. S. Baig, C. Xue, Masroor Alam, Naeem Ullah, M. Alam, S. A. Hussain

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First-Principles Simulation of the Interaction Between DNA Nucleotides and One-Dimensional Carbon Chain in Electrical Based Sequencing by Zeina Salman, Jin-Woo Kim, Steve Tung

Subjects: “…DNA sequencing…”
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The genome sequence of a leafhopper, Allygus modestus Scott, 1876 [version 1; peer review: 2 approved] by Keith Fowler

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Decoding the m6A epitranscriptomic landscape for biotechnological applications using a direct RNA sequencing approach by Chuwei Liu, Heng Liang, Arabella H. Wan, Min Xiao, Lei Sun, Yiling Yu, Shijia Yan, Yuan Deng, Ruonian Liu, Juan Fang, Zhi Wang, Weiling He, Guohui Wan

“…Traditional computational methods for detecting m6A from Nanopore direct RNA sequencing (DRS) data are constrained by their reliance on experimentally validated labels, often resulting in the underestimation of modification sites. …”
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