Leveraging Next-Generation Sequencing Application from Identity to Purity Profiling of Nucleic Acid-Based Products by Rucha Wadapurkar, Swarda Deo, Renuka Khanzode, Ajay Singh

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Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide. by Justyna Jarczak, Kamila Bujko, Katarzyna Brzeźniakiewicz-Janus, Mariusz Ratajczak, Magdalena Kucia

“…Libraries were prepared from an extremely small number of cells; however, their good quality was preserved, and they met the criteria for sequencing. We present here a step-by-step NGS protocol for sequencing VSELs and HSC with a description of troubleshooting during library preparation and sequencing.…”
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An Unusual Case of Streptococcus anginosus Group Pyomyositis Diagnosed Using Direct 16S Ribosomal DNA Sequencing by Andrew Walkty, John M Embil, Kim Nichol, James Karlowsky

“…The diagnosis was ultimately confirmed using 16S ribosomal DNA sequencing of purulent fluid obtained from a muscle abscess aspirate. …”
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Genetic diversity and transmission pattern of multidrug-resistant tuberculosis based on whole-genome sequencing in Wuhan, China by Liqing Wei, Jun Chen, Zhen Deng, Zefang Zhang, Zhengbin Zhang, Qionghong Duan

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The first complete mitochondrial genome sequence of the common Baya weaverbird (Ploceus philippinus) from southern India by Venkatesh Nagarajan-Radha, Subanithi-Purnima Murugan, Paramanantha Swami Doss Devaraj

“…The mitochondrial genome sequence would benefit future genetic studies in weaverbirds.…”
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Genetic Diversity of the Collection of Far Eastern <i>Actinidia</i> spp. Revealed by RAD Sequencing Technology by Natalia Slobodova, Maria Gladysheva-Azgari, Fedor Sharko, Kristina Petrova, Eugenia Boulygina, Svetlana Tsygankova, Irina Mitrofanova

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Assessment of Spoilage Microbiota of Rainbow Trout (Oncorhynchus mykiss) during Storage by 16S rDNA Sequencing by Guangqing Du, Yuanming Gai, Hui Zhou, Shaoping Fu, Dawei Zhang

“…Storage at 0 °C can effectively slow down the spoilage process with bacterial counts and TVB-N contents decreased from 8.7 log CFU/g and 18.7 mg/100 g obtained at 4 °C to 5.6 log CFU/g and 14.5 mg/100 g, respectively. 16S rDNA high-throughput sequencing results showed that the diversity of microbial genera decreased during storage. …”
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Fuzzy Mixed Assembly Line Sequencing and Scheduling Optimization Model Using Multiobjective Dynamic Fuzzy GA by Farzad Tahriri, Siti Zawiah Md Dawal, Zahari Taha

“…A new multiobjective dynamic fuzzy genetic algorithm is applied to solve a fuzzy mixed-model assembly line sequencing problem in which the primary goals are to minimize the total make-span and minimize the setup number simultaneously. …”
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MaterniCode: New Bioinformatic Pipeline to Detect Fetal Aneuploidies and Rearrangements Using Next-Generation Sequencing by Federico Gabrielli, Filomena Tiziana Papa, Fabio Di Pietro, Andreu Paytuví-Gallart, Daniel Julian, Walter Sanseverino, Cinzia Alfonsi

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Hematological- and Neurological-Expressed Sequence 1 Gene Products in Progenitor Cells during Newt Retinal Development by Tatsushi Goto, Fumio Tokunaga, Osamu Hisatomi

“…We found that hematological- and neurological-expressed sequence 1 (Hn1) gene was induced in depigmented retinal pigment epithelial (RPE) cells, and its expression was maintained at later stages of newt retinal regeneration. …”
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Single-cell sequencing provides clues about the developmental genetic basis of evolutionary adaptations in syngnathid fishes by Hope M Healey, Hayden B Penn, Clayton M Small, Susan Bassham, Vithika Goyal, Micah A Woods, William A Cresko

“…We created a single-cell RNA sequencing atlas of Gulf pipefish embryos to understand the developmental basis of four traits: derived head shape, toothlessness, dermal armor, and male pregnancy. …”
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Effects of stacking sequence of the layers on deformation behavior and microstructure evolution of Ti/Ni laminated metal composites by Jie Zhao, Bing Zhang, Tianli Zhao, Zhijuan Zhang, Zhiqiang Lei, Zhaolin Wang, Zengwen Zhang, Shancheng Zhan, Longjie Dang, Kuaishe Wang

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Methylated RNA Immunoprecipitation Sequencing Reveals the m6A Landscape in Oral Squamous Cell Carcinoma by Xi Wang, Jie Wu, Leyu Zhang, Wei Zhao, Jiayin Deng

“…Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A landscape in OSCC and corresponding controls. …”
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Autozygome sequencing expands the horizon of human knockout research and provides novel insights into human phenotypic variation. by Ahmed B Alsalem, Anason S Halees, Shamsa Anazi, Shomoukh Alshamekh, Fowzan S Alkuraya

“…To test this hypothesis, we exome sequenced 77 well phenotyped individuals born to first cousin parents in search of genes that are biallelically inactivated. …”
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Complete genome sequence of Pseudarthrobacter sp. NIBRBAC000502770 from coal mine of Hongcheon on Republic of Korea by Min-Kyu Park, Yeong-Jun Park, Myung-Suk Kang, Min-Ha Kim, Soo-Young Kim, Jae-Ho Shin

Subjects: “…Whole genome sequencing…”
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Resolving Rapid Radiation of Voles and Lemmings (Arvicolinae: Cricetinae, Rodentia) with QuaddRAD Sequencing and Transcriptome Analysis by Natalia Abramson, Elizaveta Skalon, Olga Bondareva, Semen Bodrov, Tatyana Petrova, Ivan Dvoyashov

“…For the first time, whole-genome sequencing data resolved relations within the third radiation wave of the subfamily’s taxa, namely the tribes Arvicolini, Lagurini, and Ellobiusini, which have formed polytomy in mitochondrial trees in earlier articles. …”
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Next generation sequencing uncovers multiple miRNAs associated molecular targets in gallbladder cancer patients by Rahul Saxena, Baskar Chakrapani, M. P. Sarath Krishnan, Amit Gupta, Sweety Gupta, Jayanta Das, Subash C. Gupta, Anissa A. Mirza, Shalinee Rao, Bela Goyal

“…GBC and control tissue samples were sequenced for miRNA expression using the Illumina HiSeq platform. …”
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Hirschsprung’s Associated Enterocolitis (HAEC) Personalized Treatment with Probiotics Based on Gene Sequencing Analysis of the Fecal Microbiome by Georg Singer, Karl Kashofer, Christoph Castellani, Holger Till

“…The fecal microbiome was measured during three healthy and three HAEC episodes by next-generation sequencing. The patient was started on daily probiotics for 3 months; the fecal microbiome was measured weekly. …”
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Moving Target Localization in Sports Image Sequence Based on Optimized Particle Filter Hybrid Tracking Algorithm by Yan Guo

“…For the image sequence S3, the tracking effect of the optimized particle filter hybrid tracking algorithm meets the requirements.…”
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Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization by Gaetano Maugeri, Maddalena Calvo, Dafne Bongiorno, Dalida Bivona, Giuseppe Migliorisi, Grete Francesca Privitera, Guido Scalia, Stefania Stefani

“…A random sequencing analysis on the most warning microorganisms could enhance sequence typing (ST) awareness within specific settings, allowing for better prevention control strategies on their eventual persistence or diffusion.…”
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