Genetic variation of the nuclear sequences of mitochondrial origin associated with retrotransposon Tv1 insertions in Drosophila species of the virilis group by B. V. Andrianov, D. A. Romanov, T. V. Gorelova

“…Mitochondrial DNA sequences integrated into chromosomes are a promising object for designing genetic markers for studies of phylogenesis and genomic instability. …”
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Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing by Chen Wenyi, Wu Xuelin, Yaqoob Muhammad Danish, Liu Kangxing, Hu Yanqing, Ke Xiuling, Hu Yongxuan

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Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections by Jiangbo Liu, Bo Yang, Yu Wu, Guihong Yang, Xiaojiu Zha, Wei Jiang

Subjects: “…metagenomic next‐generation sequencing…”
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De Novo Transcriptome Sequencing of the Orange-Fleshed Sweet Potato and Analysis of Differentially Expressed Genes Related to Carotenoid Biosynthesis by Ruijie Li, Hong Zhai, Chen Kang, Degao Liu, Shaozhen He, Qingchang Liu

“…In this study, the transcriptome profiles of an orange-fleshed sweet potato cultivar “Weiduoli” and its mutant “HVB-3” with high carotenoid content were determined by using the high-throughput sequencing technology. A total of 13,767,387 and 9,837,090 high-quality reads were produced from Weiduoli and HVB-3, respectively. …”
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Human Genomic Loci Important in Common Infectious Diseases: Role of High-Throughput Sequencing and Genome-Wide Association Studies by Gerald Mboowa, Ivan Sserwadda, Marion Amujal, Norah Namatovu

“…Application of genome sequencing technologies and genomics has enabled us to interrogate the host-pathogen interface for improving human health. …”
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Candidate genes for domestication and resistance to cold climate according to whole genome sequencing data of Russian cattle and sheep breeds by N. S. Yudin, D. M. Larkin

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Melanoma Mimicking Malignant Peripheral Nerve Sheath Tumor with Spread to the Cerebellopontine Angle: Utility of Next-Generation Sequencing in Diagnosis by Katie Fox Hanson, Paul Birinyi, Ronald Walker, Constantine Raptis, Rebecca Chernock, Jeroen Coppens, Katherine E. Schwetye

“…Over a year after resection of the parotid mass, both a cutaneous primary lentigo maligna melanoma and a metastatic CP angle melanoma were diagnosed in the same patient, prompting reconsideration of the diagnosis in the original parotid-region mass. Next-generation sequencing of a panel of cancer-associated genes demonstrated 19 identical, clinically significant mutations as well as a high tumor mutation burden in both the parotid-region and CP angle tumors, indicating a metastatic relationship between the two and a melanocytic identity of the parotid-region tumor.…”
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New Results on I2-Statistically Limit Points and I2-Statistically Cluster Points of Sequences of Fuzzy Numbers by Ö. Kişi, M. B. Huban, M. Gürdal

“…In this paper, some existing theories on convergence of fuzzy number sequences are extended to I2-statistical convergence of fuzzy number sequence. …”
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Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing by Mu-Jung Chou, Chih-Hung Cheng, Hui-Ching Wang, Ming-Ju Tsai, Chau-Chyun Sheu, Wei-An Chang

“…<b>Background:</b> For investigating the host response in <i>Acinetobacter baumannii</i> associated pneumonia, we analyzed the host genetic sequences obtained from metagenomic next-generation sequencing (mNGS). …”
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Single-cell RNA sequencing reveals the heterogeneity and interactions of immune cells and Müller glia during zebrafish retina regeneration by Hui Xu, Lining Cao, Yuxi Chen, Cuiping Zhou, Jie Xu, Zhuolin Zhang, Xiangyu Li, Lihua Liu, Jianfeng Lu

“…Here, we used single-cell RNA sequencing to investigate the cell heterogeneity and interactions of MG and immune cells in the regenerating zebrafish retina. …”
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Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening by Channabasavaiah B. Gurumurthy, Poonam S. Joshi, Scott G. Kurz, Masato Ohtsuka, Rolen M. Quadros, Donald W. Harms, K. C. Kent Lloyd

“…Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation. …”
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Exploring changes in metabolites and fecal microbiota of advanced gastric cancer based on plasma metabolomics and 16S rDNA sequencing by Xinyi Feng, Yu Zhang, Jun Feng, Zhongjun Li, Zhi Zhang, Lin Zhu, Ruoyu Zhou, Haibo Wang, Xiaojun Dai, Yanqing Liu

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Decision-Making on the Solution of a Stochastic Nonlinear Dynamical System of Kannan-Type in New Sequence Space of Soft Functions by Meshayil M. Alsolmi, Awad A. Bakery

“…In this paper, we construct and investigate the space of weighted Gamma matrix of order r in Nakano sequence space of soft functions. The idealization of the mappings has been achieved through the use of extended s-soft functions and this sequence space of soft functions. …”
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The genome sequence of Radford’s Flame Shoulder, Ochropleura leucogaster (Freyer, 1831) [version 1; peer review: 2 approved] by Mark J. Sterling, David C. Lees

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Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses by Chairat Tantrawatpan, Kotchaphon Vaisusuk, Chrysantus M. Tanga, Warayutt Pilap, Naruemon Bunchom, Ross H. Andrews, Tongjit Thanchomnang, Wanchai Maleewong, Weerachai Saijuntha

“…Genomic diversity, heterozygosity, DNA recombination, and haplotype network were calculated using AkInt3 sequences. Low polymorphism in the <i>COI</i> sequence divided <i>B. globosus</i> into six haplotypes (C1–C6). …”
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Identification of an Autophagy-Related Signature Based on Whole Bone Marrow Sequencing for the Prognosis and Immune Microenvironment Characterization of Multiple Myeloma by Licheng Li, Ting Chen, Jishi Wang, Mengxing Li, Qinshan Li

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Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing by Rica Zinsky, Servet Bölükbas, Holger Bartsch, Joachim Schirren, Annette Fisseler-Eckhoff

“…Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to the commonly used DNA sequencing was checked. We examined KRAS mutations in exon 2 codons 12 and 13 with DNA sequencing and SNaPshot analysis in 100 formalin-fixed paraffin-embedded tumor tissue samples of pancreatic carcinoma, colorectal carcinoma, and nonsmall cell lung cancer specimens of the primary tumor or metastases. 40% of these samples demonstrated mutated KRAS genes using sequencing and SNaPshot-analysis; additional five samples (45/100) were identified only with the SNaPshot. …”
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Transcriptome, miRNA, and degradome sequencing reveal the leaf stripe (Pyrenophora graminea) resistance genes in Tibetan hulless barley by Yue Wang, Qian Hu, Youhua Yao, Yongmei Cui, Yixiong Bai, Likun An, Xin Li, Baojun Ding, Xiaohua Yao, Kunlun Wu

“…Using RNA-seq, small RNA sequencing (miRNA-seq) combined with degradome sequencing (degradome-seq), four pairs associated with leaf-stripe miRNAs and target genes were obtained, namely Hvu-miR168-5p and Argonaute1 (HvAGO1), Hvu-novel-52 and growth-regulating factor 6 (HvGRF6), Hvu-miR6195 and chemocyanin-like protein (CLP), and Hvu-miR159b and gibberellin-dependent MYB (GAMYB). …”
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Exome sequencing reveals a rare damaging variant in GRIN2C in familial late-onset Alzheimer's disease by Elisa Rubino, Maria Italia, Elisa Giorgio, Silvia Boschi, Paola Dimartino, Tommaso Pippucci, Fausto Roveta, Clara Maria Cambria, Gabriella Elia, Andrea Marcinnò, Salvatore Gallone, Ekaterina Rogaeva, Flavia Antonucci, Alfredo Brusco, Fabrizio Gardoni, Innocenzo Rainero

“…Genomic DNA from family members was extracted and initially screened for pathogenic mutations in APP, PSEN1, and PSEN2, and screened for 77 genes associated with neurodegenerative conditions using NeuroX array assay. Exome sequencing was performed on three affected individuals and two healthy relatives. …”
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Combined analysis of 16S rRNA gene sequencing data reveals core vaginal bacteria across livestock species by Lucille C. Jonas, Lucille C. Jonas, Curtis R. Youngs, Stephan Schmitz-Esser, Stephan Schmitz-Esser

“…To address this variability, we conducted a combined analysis of publicly available 16S rRNA gene amplicon sequencing datasets to reveal the core vaginal microbiota in cattle, sheep, and pigs. …”
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