Is The SARS-CoV2 Evolved in Human Being: A prospective Genetic Analysis by Salvatore Dimonte, Paywast Jamal Jalal, Hiwa Abdulrahman Ahmad, Safa Bakr Karim, Taib Ahmed Hama Soor, Salar Ibrahim Ali, Muhammed Babakir-Mina, Francesco Greco

“…The phylogenetic trees for all human coronaviruses with the novel Coronavirus have been built using a several complete amino acid sequences of the four known structural proteins, S (spike), E (envelope), M (membrane), and N (nucleocapsid). …”
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miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression by Feng Tian, Ping Tang, Zhilian Sun, Ruifen Zhang, Danhua Zhu, Junying He, Jixing Liao, Qinghua Wan, Jie Shen

“…Macrophage-derived exosomes impaired glucose uptake and mitochondrial CIV complex activity and suppressed NADH dehydrogenase ubiquinone 1 alpha subcomplex 4 (NDUFA4) expression in 3T3-L1 adipocytes. miR-210 directly bind with mRNA sequences of NDUFA4 gene. Inhibition of miR-210 mitigated the effects of macrophage-derived exosomes on the glucose uptake and complex IV (CIV) activity in 3T3-L1 adipocytes, and NDUFA4 overexpression offset the inhibition of glucose uptake and CIV activity by macrophage-derived exosomes. …”
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Unleashing the potential of a low CpG Passer transposon for superior CAR-T cell therapy by Jianyao Zeng, Yan Sun, Yan Sun, Yuan Fang, Xiaodie Wang, Qian Huang, Pingjing Zhang, Meiqi Shao, Pei Wang, Jingbo Cheng, Meng Di, Tao Liu, Qijun Qian, Qijun Qian, Qijun Qian

“…Nonetheless, non-methylated CpG sequences within plasmid DNA can elicit an inflammatory response via Toll-like receptor 9 (TLR9) during CAR-T cell preparation, adversely affecting transgene expression. …”
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Сandidate SNP-markers of rheumatoid arthritis that can significantly alter the affinity of the TATA-binding protein for human gene promoters by I. V. Chadaeva, D. A. Rasskazov, E. B. Sharypova, I. A. Drachkova, E. A. Oshchepkova, L. K. Savinkova, P. M. Ponomarenko, M. P. Ponomarenko, N. A. Kolchanov, V. A. Kozlov

“…That is why, RA fits the main idea of postgenomic predictive-preventive personalized medicine that is to give a chance to those who would like to reduce his/her risk of diseases by bringing his/her conditions and lifestyle in line with the data on his/her genome sequenced. This is very important, since doctors consider RA as one of the most frequent causes of disability. …”
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Multi-omics mechanical analysis of gut microbiota, carboxylic acids, and cardiac gene expression interaction triggering diabetic cardiomyopathy by Meixin Shi, Bingbing Zhao, Wenjie Cai, Hui Yuan, Xiao Liang, Zhitao Li, Xinyu Liu, Ye Jin, Xi Liu, Can Wei

“…In this study, metagenomics, metabolomics, and transcriptomics were chosen to sequence the changes in gut microbiota, serum metabolite levels, and differentially expressed genes (DEGs) in leptin receptor-deficient db/db mice and analyze the correlation between serum metabolites and gut microbiota or DEGs. …”
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Elevated Serum Levels of the Antiapoptotic Protein Decoy-Receptor 3 Are Associated with Advanced Liver Disease by Giorgos Bamias, Michalis Gizis, Ioanna Delladetsima, Eyfrosyni Laoudi, Spyros I. Siakavellas, Ioannis Koutsounas, Garyfallia Kaltsa, John Vlachogiannakos, Irene Vafiadis-Zouboulis, George L. Daikos, George V. Papatheodoridis, Spiros D. Ladas

“…Serum DcR3 (sDcR3) levels during the chronic hepatitis/cirrhosis/hepatocellular carcinoma (HCC) sequence have not been explored. Objective. To assess the levels and significance of sDcR3 protein in various stages of chronic liver disease. …”
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Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection by David Espinosa-Ramos, Diana Caballero-Hernández, Ricardo Gomez-Flores, Armando Trejo-Chávez, Luis Jerónimo Pérez-Limón, Myriam Angélica de la Garza-Ramos, Reyes Tamez-Guerra, Patricia Tamez-Guerra, Cristina Rodriguez-Padilla

“…In the present study, a H. pylori 50–52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against H. pylori infection. …”
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Molecular Characterization of the Tumor Suppressor Candidate 5 Gene: Regulation by PPARγ and Identification of TUSC5 Coding Variants in Lean and Obese Humans by Trina A. Knotts, Hyun Woo Lee, Jae Bum Kim, Pieter J. Oort, Ruth McPherson, Robert Dent, Keisuke Tachibana, Takefumi Doi, Songtao Yu, Janardan K. Reddy, Kenji Uno, Hideki Katagiri, Magdalena Pasarica, Steven R. Smith, Dorothy D. Sears, Michel Grino, Sean H. Adams

“…Chromatin immunoprecipitation (ChIP) experiments confirmed that PPARγ protein binds a ∼−1.1 kb promotor sequence of murine TUSC5 transiently during 3T3-L1 adipogenesis, concurrent with histone H3 acetylation. …”
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<i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO₃ Stress by Regulating Root Morphology by Mengyu Liu, Jixiang Tang, Shengjie Ma, Yujing Liu, Xiaoyu Wang, Xinlei Du, Xiaohuan Sun, Yucheng Zeng, Yulong Zeng, Xiaodong Ding, Yingpeng Han, Junfeng Zhang, Lei Cao

“…Wild soybean was treated with abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA), and jasmonic acid (JA), and <i>GsEXPA8</i> was found to respond to ABA and IAA signals. Sequence analysis shows that <i>GsEXPA8</i> has DPBB_EXPA and expansin domains. …”
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DEmiRNA-mRNA regulatory network reveals miR‐122-5p as a regulatory factor of arginine metabolism in necrotizing enterocolitis by Zhili Ding, Ting Guo, Qiang Tang, Yaqiang Hong, Zhibao Lv, Li Lu, Wenjun Zhuang

“…This study aimed to explore the mechanism of miR-122-5p in NEC.MethodsDifferentially expressed (DE) miRNAs were sequenced in control and NEC mice. The DEmiRNA-mRNA regulatory network was constructed and the bioinformatics analysis was performed to identify the target mRNAs and potential roles of the DEmiRNAs. …”
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Impact of non-invasive oxygen reserve index versus standard SpO2 monitoring on peripheral oxygen saturation during endotracheal intubation in the intensive care unit: Protocol for... by Hugo Hille, Aurélie Le Thuaut, Pierre Asfar, Quentin Quelven, Emmanuelle Mercier, Anthony Le Meur, Jean-Pierre Quenot, Virginie Lemiale, Grégoire Muller, Martin Cour, Alexis Ferré, Asael Berge, Anaïs Curtiaud, Maxime Touron, Gaetan Plantefeve, Jean-Charles Chakarian, Jean-Damien Ricard, Gwenhael Colin, Arthur Orieux, Patrick Girardie, Mathieu Jozwiak, Manon Rouaud, Camille Juhel, Jean Reignier, Jean-Baptiste Lascarrou, CRICS-TRIGGERSEP Network

“…Baseline parameters, rapid-sequence induction medications, ETI devices, and physiological data are recorded. …”
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Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding by Alica Ohnesorge, Uwe John, Lucie Kuczynski, Stefan Neuhaus, Kingsly Chuo Beng, Bernd Krock, Silke Laakmann

“…DNA was extracted from eDNA and ZP samples, amplified using two universal primers that target of the mitochondrial cytochrome c oxidase subunit 1 (COI) and the nuclear ribosomal 18S rRNA genes, and paired‐end sequenced on Illumina Miseq. Metabarcoding detected 279 metazoan species (from 16 phyla) of which > 87% are known from the study area or adjacent regions. …”
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Lipid Deposition in Skeletal Muscle Tissues and Its Correlation with Intra-Abdominal Fat: A Pilot Investigation in Type 2 Diabetes Mellitus by Manoj Kumar Sarma, Andres Saucedo, Suresh Anand Sadananthan, Christine Hema Darwin, Ely Richard Felker, Steve Raman, S. Sendhil Velan, Michael Albert Thomas

“…We acquired the 2D MR spectra of calf muscles using an enhanced accelerated 5D echo-planar correlated spectroscopic imaging (EP-COSI) technique and abdominal MRI with breath-hold 6-point Dixon sequence. <b>Results:</b> In YHC, choline levels were lower in the gastrocnemius (GAS) and soleus (SOL) muscles but higher in the tibialis anterior (TA) compared to AMHC. …”
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Effective Facial Expression Recognition System Using Artificial Intelligence Technique by Imad S. Yousif, Tarik A. Rashid, Ahmed S. Shamsaldin, Sabat A. Abdulhameed, Abdulhady Abas Abdullah

“…A hybrid ANN-GA model that uses Petri Nets and production systems is proposed for the real-time video sequence analysis with high precision in predicting different dynamic facial activities of anger, surprise, disgust, joy, sadness and fear from emotion faces. …”
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An association between type A porcine endogenous retrovirus copy number and hematological parameters and gender in miniature pigs by R. B. Aitnazarov, S. V. Nikitin, G. V. Kontsevaya, M. I. Voevoda, N. S. Yudin

“…Based on the nucleotide sequence of the envelope gene (env), three main types of pig retrovirus, PERV-A, PERV-B and PERV-C, have been recognized, with PERV-A and PERV-B having the capability of infecting human cell lines in vitro. …”
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Predicting the Most Deleterious Missense Nonsynonymous Single-Nucleotide Polymorphisms of Hennekam Syndrome-Causing CCBE1 Gene, In Silico Analysis by Khyber Shinwari, Liu Guojun, Svetlana S. Deryabina, Mikhail A. Bolkov, Irina A. Tuzankina, Valery A. Chereshnev

“…Structural homology-based methods and sequence homology-based tools revealed that 8.8% of the nsSNPs are pathogenic. …”
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High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren by Xinyi Jiang, Jiacheng Shen, Peng Lin, Youming Hou

“…To understand whether the highly expressed CYPs in the antennae play an olfactory function in <i>Solenopsis invicta</i> worker, in this study, we find six highly expressed antennal CYPs from the transcriptome of <i>S. invicta</i>. Multiple sequence alignment and phylogenetic analysis divided them into two families: the CYP3 family (<i>SinvCYP6K1</i>, <i>SinvCYP6K1-1</i>) and the CYP4 family (<i>SinvCYP4C1</i>, <i>SinvCYP4C1-1</i>, <i>SinvCYP4C1-2</i>, <i>SinvCYP4V2</i>). …”
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Phylogenomic analysis reveals exceptions to the co-evolution of ZAR1 and ZRK immune gene families in plants by Li Yang, Shengyi Liu, M. Eric Schranz, Klaas Bouwmeester

“…Conclusion This study provides novel findings on the evolution of ZAR1 and ZRK immune gene families across a wide range of plant species, suggesting that more potential exceptions can be expected when expanding the list of sequenced genomes from distinct orders. Our results provide new hypotheses about the origin and diversification of these critical immune genes for future functional studies.…”
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The relationship between gallstone disease and the genetic variants of the sterol transporter adenosine triphosphate–binding cassette G8 and G5 in Egyptians by Menna A. Hassan, Salwa M. Abo El-Khair, Noha M. Mesbah, Dina M. Abo-Elmatty, Asmaa R. Abdel-Hamed

“…These polymorphisms result from nucleotide substitutions in the gene sequences, which affect the transporter’s ability to efficiently regulate cholesterol secretion into the bile. …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…For T cell repertoireβ-CDR3 analyses, T cell CDR3 was sequenced from gDNA isolated from splenocytes and enzymatically digested tumors.Results We report that activation of STING within the TME leads to slowed melanoma growth in association with increased production of antiangiogenic factors including Tnfsf15 (Vegi) and Cxcl10, and TLS-inducing factors including Ccl19, Ccl21, Lta, Ltb and Light. …”
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