Candidate vaccine construction against tick-born encephalitis based on hybrid recombinant flagG-protE-protein by P. A. Belavin, D. A. Kunyk, E. V. Protopopova, V. B. Loktev, E. V. Deineko

“…This should ensure the formation of neutralizing antibodies, and the presence of a complete amino acid sequence of protein E in the recombinant protein induces the formation of a T­cell immune response. …”
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Maximizing Identification Precision of Hymenoptera and Brachycera (Diptera) With a Non‐Destructive DNA Metabarcoding Approach by Isabel C. Kilian, Ameli Kirse, Ralph S. Peters, Sarah J. Bourlat, Vera G. Fonseca, Wolfgang J. Wägele, Andrée Hamm, Ximo Mengual

“…The study evaluated four distinct approaches, namely clustering to Amplicon Sequence Variants (ASVs) or ASVs clustered to Operational Taxonomic Units (OTUs) coupled with subsequent filtering using the LULU algorithm at 84% and 96% minimum match. …”
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New View on the Genesis of the Bashuihe Pluton, Laoshan Granites, China: Indications from Fluid Inclusions and H–O Isotopes by Huimin Liu, Zhaojun Song, Hongbo Yan, Wenyu Wang, Xinru Wang, Yifang Sun, Haonan Li

“…Primary oval caves in the Bashuihe pluton most likely evolved in the following sequence: fluid was enriched in the late diagenetic stage, diagenetic minerals crystallised under low temperature and pressure conditions, the crystallisation rate accelerated, and the magma condensed rapidly. …”
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Genetic Background and Phenotypic Aspects of Light-Induced Carotenoid Biosynthesis in <i>Isosphaeraceae</i> Planctomycetes by Anastasia A. Ivanova, Igor Y. Oshkin, Aleksandr A. Ashikhmin, Olga V. Danilova, Svetlana N. Dedysh

“…The genes involved in the carotenoid biosynthetic pathways of strain Ch08, a non-pigmented planctomycete <i>Singulisphaera acidiphila</i> MOB10^T, and all described <i>Isosphaeraceae</i> members with determined genome sequences were analyzed. The biosynthesis of C30 carotenoids via squalene, as evidenced by the presence of <i>hpnCDE</i> and <i>crtNPQO</i> gene clusters, was encoded in genomes of all pigmented <i>Singulisphaera</i>, <i>Aquisphaera</i>, and <i>Paludisphaera</i> species. …”
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Experimental investigation on the seismic resistance of RC columns after acid rain corrosion by Lihua Niu, Shansuo Zheng, Junfeng Guan, Lielie Li, Haorui Xie, Shaohua Gao

“…Therefore, this study explored the impact of acid-rain corrosion extent and axial compression ratio on the seismic behavior of RC columns that experienced flexural failure using an artificial rapid corrosion method and pseudo-static test in sequence. The corrosion-induced damage, weight loss of the reinforcement, failure pattern, hysteretic behavior, load-bearing capacity, deformation capacity, stiffness degradation, and energy dissipation were analyzed. …”
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Introducción de la técnica PCR-RFLP para el diagnóstico de dos mutaciones en el gen VHL by Antonio Alejandro Esperón, Inés Virginia Noa Hechavarría, Lídice Reyes Navarro

“…<br /> <strong>Métodos</strong>: se empleó el programa informático CLC Sequence viewer 6.5.1 para identificar enzimas de restricción cuyos sitios de corte resultaran modificados por las mutaciones c.362A&gt;G en el exón 2 y c.481C&gt;T en el exón 3 del gen VHL. …”
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Morphological and phylogenetic features of the Crimean population of <i>Juniperus deltoids</i> R.P. Adams by A. O. Lantushenko, O. O. Korenkova, A. A. Syrovets, Ya. V. Meger, P. A. Korenkov, O. M. Shevchuk

“…Studies have shown that the nuclear regions of genes have greater variability than chloroplast regions. The sequences obtained in this work formed a clade with J. deltoides samples 9430 and 9431 (BAYLU) growing in Turkey, which makes it possible to assign the samples studied to J. deltoides.…”
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Hairy transcriptional repression targets and cofactor recruitment in Drosophila. by Daniella Bianchi-Frias, Amir Orian, Jeffrey J Delrow, Julio Vazquez, Alicia E Rosales-Nieves, Susan M Parkhurst

“…As expected of direct targets, all of the putative Hairy target genes tested show Hairy-dependent expression and have conserved consensus C-box-containing sequences that are directly bound by Hairy in vitro. …”
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CFO-CR: Carrier Frequency Offset Methodology for High-Rate Common Randomness Generation by Prashanth Kumar Herooru Sheshagiri, Martin Reisslein, Juan A. Cabrera, Frank H. P. Fitzek

“…Common Randomness (CR) provides sequences of random variables at two physically separated locations. …”
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GlucoGenes^®, a database of genes and proteins associated with glucose metabolism disorders, its description and applications in bioinformatics research by V. V. Klimontov, K. S. Shishin, R. A. Ivanov, M. P. Ponomarenko, K. A. Zolotareva, S. A. Lashin

“…The results of the analysis revealed a significant increase (up to 40 %) in the proportion of genes with phylostratigraphic age index (PAI) values corresponding to the time of origin of multicellular organisms. Analysis of sequence conservation using the divergence index (DI) showed that most of the corresponding genes are highly conserved (DI &lt; 0.6) or conservative (DI &lt; 1). …”
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Surveillance and characteristics of vancomycin-resistant Enterococcus isolates in a Chinese tertiary hospital in Shenzhen, 2018 to 2024 by Hongwei Shen, Qiaomin Zhang, Shaobo Li, Tingting Huang, Wen Ma, Daming Wang, Peng Li

“…The VRE isolates were identified and subjected to screening for the six van genes and multi-locus sequence typing (MLST) genotyping. The clinical characteristics, antimicrobial susceptibility profiles and molecular features were subjected to analysis. …”
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Comparative analysis of lactaptin activity when produced in bacterial or eukaryotic expression systems by O. A. Koval, O. Y. Volkova, A. A. Gorchakov, S. V. Kulemzin, A. V. Tkachenko, A. A. Nushtaeva, E. V. Kuligina, V. A. Richter, A. V. Taranin

“…Using lentiviral vector pCDH as a backbone, two constructs, pEL1 and pEL2, encoding secreted forms of lactaptin that differ in their signal sequences were created. Lactaptin expression in human cell lines was confirmed using Western-blot analysis, whereas ELISA was used for quantification of secreted lactaptin. …”
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“I lock myself in my room and cry in frustration”: an analysis of adolescent behaviors of child-to-parent violence by Shirley Arias-Rivera, Barbara Lorence, Barbara Lorence, Jesus Maya

“…Following thematic analysis, adolescents’ responses were categorized into 6 themes and 17 sub-themes in mild cases. A possible sequence in adolescents’ responses was identified, divided into three phases. …”
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Altered expression profile of plasma exosomal microRNAs in exclusive electronic cigarette adult users by Dongmei Li, Zidian Xie, Sadiya Bi Shaikh, Irfan Rahman

“…We used plasma samples from 15 exclusive e-cigarette users and 15 non-users from the Population Assessment of Tobacco and Health (PATH) Wave 1 study (2013–2014) and sequenced miRNAs with Illumina NextSeq 500/550. We performed differential analyses using DESeq2 in R/Bioconductor, adjusting for race, and conducted gene enrichment analyses on target genes regulated by significant miRNAs. …”
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Exploring diazotrophic diversity: unveiling Nif core distribution and evolutionary patterns in nitrogen-fixing organisms by Bruno Thiago de Lima Nichio, Roxana Beatriz Ribeiro Chaves, Fábio de Oliveira Pedrosa, Roberto Tadeu Raittz

“…Results In a systematic study, 118 diazotrophs were identified, resulting in a database of 2,156 Nif protein sequences obtained with RAFTS³G. Using this Nif database and a data mining strategy, we extended our analysis to 711 species and found that 544 contain the Nif core. …”
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Petrophysical Regression regarding Porosity, Permeability, and Water Saturation Driven by Logging-Based Ensemble and Transfer Learnings: A Case Study of Sandy-Mud Reservoirs by Shenghan Zhang, Yufeng Gu, Yinshan Gao, Xinxing Wang, Daoyong Zhang, Liming Zhou

“…From a general review, most petrophysical models applied for the conventional logging interpretation imply that porosity, permeability, or water saturation mathematically have a linear or nonlinear relationship with well logs, and then arguing the prediction of these three parameters actually is accessible under a regression of logging sequences. Based on this knowledge, ensemble learning technique, partially developed for fitting problems, can be regarded as a solution. …”
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Assessing cell lines with inducible depletion of cohesin and condensins components through analysis of metaphase chromosome morphology by A. M. Yunusova, A. V. Smirnov, I. E. Pristyazhnuk, T. A. Shnaider, E. K. Maltseva, S. D.  Afonnikova, O. A. Gusev, N.  R. Battulin

“…To use this system, it is sufficient to introduce a transgene encoding a plant-derived auxin-dependent ubiquitin ligase into mammalian cells and insert a sequence encoding a degron domain into the gene of interest. …”
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Egfr Amplification Specific Gene Expression in Phyllodes Tumours of the Breast by Konstantin Agelopoulos, Christian Kersting, Eberhard Korsching, Hartmut Schmidt, Arno Kuijper, Christian August, Pia Wülfing, Joke Tio, Werner Boecker, Paul J. van Diest, Burkhard Brandt, Horst Buerger

“…Conclusion: Our results show that amplifications within regulatory sequences of egfr are associated with the expression of eps15 and caveolin-1, indicating an increased turnover of EGFR. …”
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Differential Production of Midkine and Pleiotrophin by Innate APCs upon Stimulation through Nucleic Acid-Sensing TLRs by Elias A. Said, Sumaya Al-Dughaishi, Wadha Al-Hatmi, Iman Al-Reesi, Mohammed S. Al-Balushi, Atika Al-Bimani, Juma Z. Al-Busaidi, Marwa Al-Riyami, Murtadha Al-Khabori, Salam Al-Kindi, Francesco A. Procopio, Shadia Al-Sinawi, Aliyaa Al-Ansari, Crystal Y. Koh, Khalid Al-Naamani, Ali A. Al-Jabri

“…Midkine (MK) and pleiotrophin (PTN) belong to the same family of cytokines. They have similar sequences and functions. Both have important roles in cellular proliferation, tumors, and diseases. …”
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Segmented evolution and exploration targets of the Cambrian platform margin in the Manxi area, Tarim Basin by Zongquan Hu, Fan Feng, Chengming Fang, Zicheng Cao, Tieyi Wang, Kangkang Guo, Yang Li, Shi Wang

“…This study determines the vertical and lateral differential evolution of the platform margin (in the Manxi area hereafter referred to as the Cambrian Manxi platform margin) through fine-scale sequence stratigraphic division and a segmented analysis. …”
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