The effect of differences in the third domain of the glycoprotein E of tick-borne encephalitis virus of the Far Eastern, Siberian and European subtypes on the binding of recombinan... by I. K. Baykov, A. L. Matveev, L. A. Emelianova, G. B. Kaverina, S. E. Tkachev, N. V. Tikunova

“…The aim of this study was to investigate the effect of amino acid sequence differences of recombinant D3 domains derived from the glycoprotein E of TBEV of the Far Eastern, Siberian and European subtypes on the binding of the protective antibody ch14D5 to these proteins. …”
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Amplitude Modulation Depth Coding Method for SSVEP-Based Brain–Computer Interfaces by Ruxue Li, Zhenyu Wang, Xi Zhao, Guiying Xu, Honglin Hu, Ting Zhou, Tianheng Xu

“…Each stimulus with a single carrier frequency was assigned a specific binary sequence to operate two modulation depths. Two experiments were conducted to comprehensively assess the effectiveness of this approach. …”
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Molecular Insights Into the Sensory Adaption of the Cave‐Dwelling Leech Sinospelaeobdella wulingensis to the Karst Cave Environment by Xi Wen, Haiyang Xiang, Mengqing Zhang, Aoran Yan, Dongqing Xiang, Jie Zou, Yue Zhang, Xinglong Huang, Zhixiao Liu

“…SwiGluRs are conserved in protein sequence and share high identities with homologous proteins in other leeches. …”
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Structural Characterization of B-DNA d(CGTGAATTCACG)₂ in Complex with the Specific Minor Groove Binding Fluorescent Marker Hoechst 33342 by Hristina Sbirkova-Dimitrova, Rusi Rusew, Hristo Gerginov, Annie Heroux, Boris L. Shivachev

“…This work focuses on the binding interactions of Hoechst 33342 with the specific DNA sequence d(CGTGAATTCACG)₂. The structure of the complex was determined using single-crystal X-ray diffraction at a resolution of 1.9 Å in the space group <i>P</i>2₁2₁2₁. …”
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Integrative genomic analyses combined with molecular dynamics simulations reveal the impact of deleterious mutations of Bcl-2 gene on the apoptotic machinery and implications in ca... by Ghazi Elamin, Ghazi Elamin, Zhichao Zhang, Depika Dwarka, Kabange Kasumbwe, John Mellem, Nompumelelo P. Mkhwanazi, Paradise Madlala, Paradise Madlala, Mahmoud E. S. Soliman

“…Therefore, this study explores the association between SNPs in Bcl-2 and the structural, functional, protein-protein interactions (PPIs), drug binding and dynamic characteristics.MethodsComprehensive cross-validated bioinformatics tools and molecular dynamics (MD) simulations. Multiple sequence, genetic, structural and disease phenotype analyses were applied in this study.ResultsAnalysis revealed that out of 130 mutations, approximately 8.5% of these mutations were classified as pathogenic. …”
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Biological and Molecular Characterization of Moroccan Isolates of <i>Spiroplasma citri</i> by Tourya Sagouti, Ikram Legrifi, Naima Rhallabi, Zineb Belabess, Moussa El Jarroudi, Said Amiri, Essaid Ait Barka, Abdessalem Tahiri, Rachid Lahlali

“…Molecular analysis showed that Moroccan isolates (27GH, 3GH, 8GH, 56MK, 16MK, 60MK, 2GLK, 13SS, and 30S1) exhibited complete (100%) sequence similarity with each other and the reference strain R2-A8. …”
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Study on seismic performance of high-strength steel earthquake-resilient beam-column joint with double damage elements by Hongchao Guo, Dongdong Zheng, Jing Lu, Xudong Zhou, Wenqi Wang, Yunhe Liu

“…The research findings show a significant time sequence in the joint double damage element, with both components dissipating over 90% of energy. …”

TARGET Protein: the effect of augmented administration of enteral protein to critically ill adults on clinical outcomes—statistical analysis plan for a cluster randomized, cross-se... by Sophie Zaloumis, Matthew J. Summers, Jeffrey J. Presneill, Rinaldo Bellomo, Lee-anne S. Chapple, Marianne J. Chapman, Adam M. Deane, Suzie Ferrie, Craig French, Sally Hurford, Nima Kakho, Matthew J. Maiden, Stephanie N. O’Connor, Sandra L. Peake, Emma J. Ridley, An Tran-Duy, Patricia J. Williams, Paul J. Young, Amalia Karahalios, on behalf of the TARGET Protein Investigators, the Australian, New Zealand Intensive Care Society Clinical Trials Group

“…Each ICU received one trial formula for a 3-month period and then switched to the alternate formulae. This sequence was repeated, for a total trial length of 12 months. …”
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Spinal cord demyelination predicts neurological deterioration in patients with mild degenerative cervical myelopathy by Jefferson R Wilson, David Anderson, Stephan Duplessis, Julien Cohen-Adad, David W Cadotte, Nathan Evaniew, Jacques Bouchard, Michael Craig, Abdul Al-Shawwa, Kalum Ost, Steve Casha, W Bradley Jacobs, Saswati Tripathy, Peter Lewkonia, Fred Nicholls, Alex Soroceanu, Ganesh Swamy, Kenneth C Thomas, Michael MH Yang, Nicholas Dea

“…This study aims to identify imaging and clinical predictors of neurological deterioration in mild DCM and explore pathophysiological correlates to guide clinical decision-making.Methods Patients with mild DCM underwent advanced MRI scans that included T2-weighted, diffusion tensor imaging and magnetisation transfer (MT) sequences, along with clinical outcome measures at baseline and 6-month intervals after enrolment. …”
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A broad spectrum Shigella vaccine based on VirG53 – 353 multiepitope region produced in a cell-free system by Girmay Desalegn, Charlotte Abrahamson, K. Ross Turbyfill, Lucy Pill-Pepe, Leslie Bautista, Chitradevi S. Tamilselvi, Dylan Dunn, Neeraj Kapoor, Barbara Sullinger, Matheo Herrera, Edwin V. Oaks, Jeff Fairman, Marcela F. Pasetti

“…Seeking to refine the vaccine antigenic target and achieve scalable manufacturing compatible with Good Manufacturing Practices, we mapped linear B-cell epitopes spanning the entire VirG protein sequence by probing the immune reactivity of 10-mer peptides (overlapping 4–8 aa) with sera from Shigella-infected rhesus monkeys. …”
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Scrutinizing the evidence of anthracene toxicity on adrenergic receptor beta-2 and its bioremediation by fungal manganese peroxidase via in silico approaches by Muhammad Naveed, Khadija Khatoon, Tariq Aziz, Rida Naveed, Muhammad Nouman Majeed, Maida Salah Ud Din, Tayyab Javed, Ayaz Ali Khan, Abdullah F. Alasmari

“…Toxicity prediction using Pro-Tox 3.0 identified anthracene as a compound of toxicity class 4 with a LD50 of 316 mg/kg. Sequence of manganese peroxidase from Lachnellula suecica and human adrenergic receptor beta 2 were retrieved from NCBI databases. …”
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Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen by Mark Hutchinson, Jeffrey A. Ruffolo, Nantaporn Haskins, Michael Iannotti, Giuliana Vozza, Tony Pham, Nurjahan Mehzabeen, Harini Shandilya, Keith Rickert, Rebecca Croasdale-Wood, Melissa Damschroder, Ying Fu, Andrew Dippel, Jeffrey J. Gray, Gilad Kaplan

“…In this study, DeepAb, a deep learning model for predicting antibody Fv structure directly from sequence, was used in conjunction with single-point experimental deep mutational scanning (DMS) enrichment data to design 200 potentially optimized variants of an anti-hen egg lysozyme (HEL) antibody. …”
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An Effective Iterative Process Utilizing Transcendental Sine Functions for the Generation of Julia and Mandelbrot Sets by Khairul Habib Alam, Yumnam Rohen, Anita Tomar, Naeem Saleem, Maggie Aphane, Asima Razzaque

“…Moreover, we apply <i>s</i>-convexity to the iteration procedure to construct orbits under convexity conditions, and we present a theorem that determines the condition when a sequence diverges to infinity, known as the escape criterion, for the transcendental sine function <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo form="prefix">sin</mo><mo>(</mo><msup><mi>u</mi><mi>m</mi></msup><mo>)</mo><mo>−</mo><mi>α</mi><mi>u</mi><mo>+</mo><mi>β</mi></mrow></semantics></math></inline-formula>, where <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>u</mi><mo>,</mo><mi>α</mi><mo>,</mo></mrow></semantics></math></inline-formula><inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>β</mi><mo>∈</mo><mi mathvariant="double-struck">C</mi></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>m</mi><mo>≥</mo><mn>2</mn></mrow></semantics></math></inline-formula>. …”
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Luteolin Pretreatment Ameliorates Myocardial Ischemia/Reperfusion Injury by lncRNA-JPX/miR-146b Axis by Tongda Xu, Yuanyuan Zhang, Gege Liao, Haochen Xuan, Jie Yin, Jieli Bao, Yang Liu, Dongye Li

“…Results. miR-146b was demonstrated to bind and recognize the JPX sequence site by dual luciferase reporter gene assay. …”
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Morphological and Molecular Identification of <i>Sarcocystis arctica</i> in Captive Cheetahs (<i>Acinonyx jubatus</i>) in China Helps Clarify Phylogenetic Relationships with <i>Sar... by Zhe Liao, Niuping Zhu, Yurong Yang, Shuangsheng Deng, Thomas Jäkel, Junjie Hu

“…Phylogenetic reconstruction revealed that the newly sequenced <i>Sarcocystis</i> clustered with <i>S. arctica</i> and <i>S. caninum</i>, whereas <i>S. felis</i> (ITS-1) and <i>S. canis</i> (ITS-1, 18S rRNA, <i>cox</i>1) were sister species. …”
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First report of non-marine ostracods (Crustacea, Ostracoda) from the Dahlak Archipelago (Eritrea), with the description of two new species by Giampaolo Rossetti, Ilaria Mazzini, Giorgio Chiozzi, Luca Vecchioni, Federico Marrone

“…A detailed description of the morphology of valves and soft parts is given, and COX1 sequences were obtained for four species. Cypris galefensis was so far only known for its type locality in Somalia with a population containing both males and females, whereas our samples consisted exclusively of females. …”
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Molecular genetic and morphological characteristics of Micractinium thermotolerans and M. inermum (Trebouxiophyceae, Chlorophyta) from pyroclastic deposits of the Kamchatka Peninsu... by R. Z. Sushchenko, V. Yu. Nikulin, V. B. Bagmet, A. Yu. Nikulin

“…Identification of the strains was carried out within the framework of an integrative approach using microscopic and molecular genetic methods, including preliminary taxon identification, obtaining nucleotide sequences of the small subunit and the internal transcribed spacer rRNA, reconstruction of phylogenetic trees and secondary structures of the ITS1 and ITS2 rRNA regions. …”
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Oxytocin as a physiological correlate of dyadic music therapy relationships — a randomized crossover pilot study by Paula Kristin Busse, Lutz Neugebauer, Götz Kaschubowski, Dennis Anheyer, Dennis Anheyer, Dennis Anheyer, Thomas Ostermann

“…The primary outcome was salivary oxytocin, with tests performed immediately before and after each 30-min intervention.ResultsThirty-two children were included in the study, resulting in n = 16 children per allocation sequence. During the implementation of the study, difficulties were encountered with protocol adherence both in terms of the duration of the music therapy and the implementation of the silent work in the control group. …”
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Late Glacial and Holocene Paleoenvironmental Reconstruction of the Submerged Karst Basin Pirovac Bay on the Eastern Adriatic Coast by Nikolina Ilijanić, Dea Brunović, Slobodan Miko, Valentina Hajek Tadesse, Ozren Hasan, Ivan Razum, Martina Šparica Miko, Saša Mesić

“…This study focuses on the analysis of sediment core retrieved from the deepest part (25 m) of Pirovac Bay. A long sedimentary sequence (7.45 m) supplemented by a shorter sediment core (1.45 m) from a shallower part of the bay was analyzed for sedimentological, mineralogical, geochemical, and micropaleontological (ostracod) parameters. …”
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DETERMINING THE LOCATION AND OPTIMAL NUMBER OF BUILDING LIFTS IN HIGH-RISE CONSTRUCTION PROJECTS USING A LINEAR INTEGER MATHEMATICAL MODEL by M. Jawaheri, M. Ahmadnia, M. Maghrebi, R. Ghanbari

“…Extensive research has been conducted on reducing lift travel time between floors, optimizing lift systems design, optimizing lift movement paths, determining the sequence of floor travel, managing lift energy consumption, and floor zoning. …”
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