Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…Ultimately, the detection capability of this method was assessed using clinical samples.ResultsThe sequenced tags, when paired with primers, could generate Tm differences exceeding 3°C. …”
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The GWAS-MAP|ovis platform for aggregation and analysis of genome-wide association study results in sheep by A. V. Kirichenko, A. S. Zlobin, T. I. Shashkova, N. A. Volkova, B. S. Iolchiev, V. A. Bagirov, P. M. Borodin, L. С. Karssen, Y. A. Tsepilov, Y. S. Aulchenko

“…The platform currently contains information on more than 34 million associations between genomic sequence variants and traits of meat production in sheep. …”
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Late-onset first epileptic seizure and cerebral small vessel disease: role of juxtacortical white matter lesions by Adrian Nasca, Adrian Nasca, Leo Sokolovič, Leo Sokolovič, Oliver Koprda, Patrick Haage, Patrick Haage, Thorsten Schmidt, Juraj Kukolja, Juraj Kukolja

“…A voxel-based morphometry (VBM) analysis was additionally performed on T1 weighted sequences using the automated SPM12 based CAT12 software.ResultsComparing intrapersonal volume ratios adjusted for the effects of gender and age, we found that the WML distribution was shifted to the juxtacortical compartment in LOFES patients. …”
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The sarcoma ring trial: a case-based analysis of inter-center agreement across 21 German-speaking sarcoma centers by Siyer Roohani, Jolina Handtke, Kamal Hummedah, Markus Albertsmeier, Dimosthenis Andreou, Leonidas Apostolidis, Marinela Augustin, Sebastian Bauer, Moritz Billner, Florian Bösch, Christoph K. W. Deinzer, Niklas Deventer, Anna Duprée, Franziska Eckert, Lars Engel, Katja Fechner, Hagen Fritzsche, Verena Gaidzik, Saeed Ghani, Robert Grützmann, Wiebke K. Guder, Rainer Hamacher, Judith S. Hecker, Anne Hendricks, Axel Hillmann, Philipp Houben, Georg Hübner, Philipp Ivanyi, Christina Jentsch, Maren Jordan, Peter Kappl, Moritz Kaths, Torsten Kessler, Johanna Kirchberg, Carolin Knebel, Robert Krempien, Burkhard Lehner, Ulrich Lenze, Lars H. Lindner, Alisa Martina Lörsch, Nadia Maguire, Sophie Müller, Pompiliu Piso, Vlatko Potkrajcic, Peter Reichardt, Stephan Richter, Simone Schewe, Lars M. Schiffmann, Felicitas Scholten, Jana Käthe Striefler, Matthias Schwarzbach, Katharina Seidensaal, Sabine Semrau, Joanna Szkandera, Christoph J. Szuszies, Beate Timmermann, Armin Tuscherer, Armin Wiegering, Moritz T. Winkelmann, David Kaul, Jens Jakob

“…Centers provided recommendations on treatment sequence and modalities, along with the consensus level within their MTB. …”
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Cell Wall Protein 2 as a Vaccine Candidate Protects Mice Against <i>Clostridioides difficile</i> Infection by Shaohui Wang, Joshua Heuler, Jessica Bullock, Junling Qin, Soumyadeep Chakraborty, Agbendeh Lubem Nathaniel, Shifeng Wang, Xingmin Sun

“…Results: Our in silico analyses predicted the immunogenic region (functional domain) of Cwp2 and revealed its high homology among various toxinotypes and ribotypes (R.T.s) or sequence types (S.T.s). Immunizations of mice with the Cwp2 functional domain (Cwp2_A) induced potent IgG/A antibody responses against Cwp2_A, protected mice from CDI, and reduced <i>C. difficile</i> spore and toxin levels in feces post-infection. …”
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A lipidated peptide derived from the C-terminal tail of the vasopressin 2 receptor shows promise as a new β-arrestin inhibitor by Rebecca L. Brouillette, Christine E. Mona, Michael Desgagné, Malihe Hassanzedeh, Émile Breault, Frédérique Lussier, Karine Belleville, Jean-Michel Longpré, Michel Grandbois, Pierre-Luc Boudreault, Élie Besserer-Offroy, Philippe Sarret

“…To address this issue, we designed and synthesised a novel lipidated phosphomimetic peptide inhibitor targeting β-arrestins, called ARIP, which was developed based on the C-terminal tail (A343-S371) of the vasopressin V2 receptor. As the V2R sequence has been shown to bind β-arrestins with high affinity, we added an N-terminal palmitate residue to allow membrane tethering and cell entry. …”
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Prevalence of Toxoplasma gondii, Neospora caninum, and Sarcocystis Species DNA in the Heart and Breast Muscles of Rock Pigeons (Columbia livia) by Muhammad Mudasser Nazir, Muhammad Mazhar Ayaz, Atif Nisar Ahmed, Azhar Maqbool, Kamran Ashraf, Muhammad Oneeb, Ghulam Yasin, Atta Subhani, Muhammad Asif Ali, Noman Nazir, Muhammad Afzal Sajid

“…Two of the positive Neospora caninum amplicons were sequenced and had 97% nucleotide identity with N. caninum isolates. …”
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Case report: Deciphering the clinical significance of a novel partial BRCA1 exon 10 duplication in a patient with triple-negative breast cancer by Alice Faversani, Debora Manuelli, Davide Barteselli, Giulia Melloni, Carlo Santaniello, Luigi Corsaro, Luigi Corsaro, Davide Sacco, Davide Sacco, Davide Clerici, Laura Gargiulo, Fulvio Ferrara, Lucy Costantino

“…RNA analysis allowed to confirm the production of an altered mRNA showing the duplicated sequence. In this way, we were able to assign a clinical significance to the new alteration and classify it as a pathogenic variant. …”
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Heterochrony in orthodenticle expression is associated with ommatidial size variation between Drosophila species by Montserrat Torres-Oliva, Elisa Buchberger, Alexandra D. Buffry, Maike Kittelmann, Genoveva Guerrero, Lauren Sumner-Rooney, Pedro Gaspar, Georg C. Bullinger, Javier Figueras Jimenez, Fernando Casares, Saad Arif, Nico Posnien, Maria D. S. Nunes, Alistair P. McGregor, Isabel Almudi

“…We discovered that the activity of an otd eye enhancer is consistent with the difference in the expression of this gene between species, with the D. mauritiana enhancer sequence driving earlier expression than that of D. simulans. …”
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Unveiling the Role of <i>GhP5CS1</i> in Cotton Salt Stress Tolerance: A Comprehensive Genomic and Functional Analysis of <i>P5CS</i> Genes by Hui Fang, Xin Gao, Yunhao Wu, Ke Zhang, Ying Wu, Junyi Li, Dongmei Qian, Ruochen Li, Haijing Gu, Teame Gereziher Mehari, Xinlian Shen, Baohua Wang

“…In this study, we identified 40 <i>P5CS</i> genes across four cotton species with diverse sequence lengths and molecular weights. Phylogenetic analysis of 100 <i>P5CS</i> genes from nine species revealed three subgroups, with <i>Gossypium hirsutum</i> closely related to <i>Gossypium barbadense</i>. …”
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UTE MRI technical developments and applications in osteoporosis: a review by Soo Hyun Shin, Hee Dong Chae, Hee Dong Chae, Arya Suprana, Arya Suprana, Saeed Jerban, Eric Y. Chang, Eric Y. Chang, Lingyan Shi, Robert L. Sah, Jeremy H. Pettus, Gina N. Woods, Jiang Du, Jiang Du, Jiang Du

“…This review article discusses using ultrashort echo time (UTE) sequences to evaluate bone composition and structure. …”
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Characteristics of Egg Production and Discovery of the Prolactin Gene in Indigenous Tropical White Leghorn Layer Chickens of Indonesia by N Pratiwi, T Sartika, Komarudin, T Haryati, T Kostaman, N Azizah, A Sukmara, B Brahmantiyo, Isbandi, H Zaenal, H Nuraini, H Khasanah, MF Ridhillah, F Saputra

“…A total of 185 WL chickens (5 batches) were utilized, with 47 DNA samples sequenced using specific primers for the PRL gene’s exons 2 and 5. …”
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Lattice-based interpenetrating phase composite metamaterial fabricated with hybrid material extrusion process for tunable mechanical properties by Ritik Raj, Jung-Ting Tsai, Mayur Jiyalal Prajapati, Jeng-Ywan Jeng

“…Overall, this study presents an innovative fabrication technique for IPC metamaterials, revealing how the strategic placement and stacking sequence of secondary materials within the primary structure significantly influences their mechanical properties.…”
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Sulforaphane Wrapped in Self-Assembled Nanomicelle Enhances the Effect of Sonodynamic Therapy on Glioma by Yihong Li, Xuejie Yang, Zhen Wei, Heng Niu, Liyang Wu, Caijing Chen, Huina Liu, Ting Cai, Huadong Fan

“…<b>Results/Conclusions:</b> SFN@RB@SPM can be internalized by the glioma cells through the tumor-targeting motif RGDS (abbreviated for the peptide sequence composed of arginine, glycine, aspartic acid, and serine), and further executes antitumor function during SDT. …”
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High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression by Jie Qi, Qichao Wu, Qian Cheng, Xiangyuan Chen, Minmin Zhu, Changhong Miao

“…E26 transformation-specific sequence transcription factor-1 (ESE-1), which belongs to the E26 transformation-specific family of transcription factors, has been demonstrated to be involved in COX2 and iNOS gene transcription. …”
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Decoding the mannose receptor-mAb interaction: the importance of high-mannose N-glycans and glycan-pairing by Julia Baumeister, Maximilian Meudt, Sybille Ebert, Frank Rosenau, Boris Mizaikoff, Michaela Blech, Kristina M. J. Aertker, Fabian Higel

“…The interaction is found to be dependent on high-mannose content, but is independent of the mAb format or sequence. However, different glyco-pairs exhibited varying binding affinities to the MR, with the symmetrical high-mannose glyco-pair showing the strongest binding properties. …”
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Protective Role of Glutathione and Nitric Oxide Production in the Pathogenesis of Pterygium by Fidelina Parra, Alexander Kormanovski, Gustavo Guevara-Balcazar, María del Carmen Castillo-Hernández, Antonio Franco-Vadillo, Mireille Toledo-Blas, Rosa Adriana Jarillo-Luna, Eleazar Lara-Padilla

“…The results are congruent with the following proposed sequence of events leading to a protective response of the organism during the pathogenesis of primary pterygium: a decreased level of eNOS provokes a decline in the level of NO in pterygium tissue, which then leads to reduced S-nitrosylation of GSH or other thiols and possibly to the modulation of the intracellular level of GSH through synthesis and/or mobilization from other tissues.…”
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A positive experience in applying the biolistic approach to potato varieties Aksor and Nevskiy by N. P. Malakhova, Y. A. Skiba, G. A. Iskakova, D. A. Naizabayeva, B. K. Tezekbaeva, G. A. Ismagulova, E. R. Maltseva

“…The main advantages of this method include the ability to simultaneously incorporate several target genes into the plant genome, carry out transfer without unnecessary agrobacterial parts and plasmid DNA sequences, and the short time needed to produce transgenic cells. …”
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Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China by Wenbo Li, Linzi Zeng, Rongmei Yuan, Teng Qi, Hongyu Liao, Yuhangxi Cao, Shu Huang, Zhiguo Liu, Zhenjun Li

“…In this study, bacteriology, conventional bio-typing, multi-locus sequence typing (MLST), and multiple locus variable-number tandem repeat analysis (MLVA) were applied to preliminarily characterize the strains in terms of genetic diversity and epidemiological links. …”
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Inhibition of Endothelial–Mesenchymal Transition Mediated by Activin Receptor Type IIA Attenuates Valvular Injury Induced by Group A Streptococcus in Lewis Rats by Zirong Lu, Yuan Li, Chuanghong Lu, Zhongyuan Meng, Ling Bai, Feng Huang, Zhiyu Zeng

“…We constructed a small interfering RNA (ACVR2A-siRNA) by silencing activin receptor type IIA (ACVR2A) and an adeno-associated virus (AAV-ACVR2A) containing a sequence that silenced ACVR2A. The EndMT cell model was established via human umbilical vein endothelial cells (HUVECs), and the RHD animal model was established via female Lewis rats. …”
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