The genome sequence of a sawfly, Abia candens Konow, 1887 [version 1; peer review: 2 approved] by Andrew Cunningham, Andrew Halstead

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Accelerating next generation sequencing data analysis: an evaluation of optimized best practices for Genome Analysis Toolkit algorithms by Karl R. Franke, Erin L. Crowgey

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Genome sequence of Novosphingobium malaysiense strain MUSC 273T, novel alpha-proteobacterium isolated from intertidal soil by Hooi-Leng Ser, Wai-Fong Yin, Kok-Gan Chan, Nurul-Syakima Ab Mutalib, Learn-Han Lee

“…The strain was isolated from intertidal soil with strong catalase activity. The genome sequence comprises 5,027,021 bp, with 50 tRNA and 3 rRNA genes. …”
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Identifying human activities causing water pollution based on microbial community sequencing and source classifier machine learning by Zhangmu Jing, Yi Zhang, Xiaoling Liu, Qingqian Li, Yanji Hao, Yeqing Li, Hongjie Gao

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Developing JSequitur to Study the Hierarchical Structure of Biological Sequences in a Grammatical Inference Framework of String Compression Algorithms by Bulgan Galbadrakh, Kyung-Eun Lee, Hyun-Seok Park

“…Grammatical inference methods are expected to find grammatical structures hidden in biological sequences. One hopes that studies of grammar serve as an appropriate tool for theory formation. …”
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Persistent functional and taxonomic groups dominate an 8,000-year sedimentary sequence from Lake Cadagno, Switzerland by Paula Rodriguez, Jasmine S. Berg, Longhui Deng, Longhui Deng, Hendrik Vogel, Michal Okoniewski, Mark A. Lever, Mark A. Lever, Cara Magnabosco

“…Here, we reconstruct the microbial functional and taxonomic composition of an 8,000-year Holocene sedimentary succession from meromictic Lake Cadagno (Switzerland) using shotgun metagenomics and 16S rRNA gene amplicon sequencing. While younger sediments (<1,000 years) are dominated by typical anaerobic surface sedimentary bacterial taxa (Deltaproteobacteria, Acidobacteria, and Firmicutes), older layers with lower organic matter concentrations and reduced terminal electron acceptor availability are dominated by taxa previously identified as “persistent populations” within deep anoxic marine sediments (Candidatus Bathyarchaeia, Chloroflexi, and Atribacteria). …”
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Diagnosis of Fanconi Anemia: Mutation Analysis by Multiplex Ligation-Dependent Probe Amplification and PCR-Based Sanger Sequencing by Johan J. P. Gille, Karijn Floor, Lianne Kerkhoven, Najim Ameziane, Hans Joenje, Johan P. de Winter

“…In addition, in populations with strong founder mutations, a limited test using Sanger sequencing and MLPA will be a cost-effective alternative. …”
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Assessing the authenticity and purity of a commercial Bacillus thuringiensis bioinsecticide through whole genome sequencing and metagenomics approaches by Yari Van Laere, Yari Van Laere, Marie-Alice Fraiture, Andrea Gobbo, Sigrid C. J. De Keersmaecker, Kathleen Marchal, Kathleen Marchal, Nancy H. C. Roosens, Kevin Vanneste

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Metagenomic Next-Generation Sequencing (mNGS) for the Timely Diagnosis of Carbapenem-Resistant Klebsiella pneumoniae in Leukemia Patients by Huan Tao, Fang Hua, Yingying Chen, Yongqian Jia

“…This report shows the contribution of metagenomic next-generation sequencing (mNGS) as an alternative to challenging diagnostic infection in immunosuppressed individuals. …”
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Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing by ZHAO Weiguo, TA Na, WANG Hao, YANG Zelin

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Long journey of 16S rRNA‐amplicon sequencing toward cell‐based functional bacterial microbiota characterization by Jianshi Jin, Xiongduo Liu, Katsuyuki Shiroguchi

Subjects: “…16S rRNA‐amplicon sequencing…”
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Single-cell RNA sequencing highlights the role of distinct natural killer subsets in sporadic amyotrophic lateral sclerosis by Esther Álvarez-Sánchez, Álvaro Carbayo, Natalia Valle-Tamayo, Laia Muñoz, Joaquim Aumatell, Soraya Torres, Sara Rubio-Guerra, Jesús García-Castro, Judit Selma-González, Daniel Alcolea, Janina Turon-Sans, Alberto Lleó, Ignacio Illán-Gala, Juan Fortea, Ricard Rojas-García, Oriol Dols-Icardo

“…However, no study has investigated the role of peripheral blood immune cells in ALS pathophysiology using single-cell RNA sequencing (scRNAseq). Methods We aimed to characterize immune cells from blood and identify ALS-related immune alterations at single-cell resolution. …”
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Single-Cell RNA Sequencing Reveals Macrophage Dynamics During MASH in <i>Leptin</i>-Deficient Rats by Xiaoming Xin, Yaohua Ni, Jing Wang, Fenglin Wu, Meichen Liu, Lingjuan Wu, Jiaxing Dai, Chenglin Wu, Xiaolei Song, Wang Zhang, Guangrui Yang, Ruling Shen, Xianmin Zhu

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Identification and Phylogenetic Tree Determination of Spodoptera frugiperda from Java Based on mtDNA COI Sequence Analysis by Ani Widarti, Enie Tauruslina, Idah Faridah, Willing Bagariang, Hadi Suyanto, Didah Mahmudah, Rista Susanti, Rosalia Maryana, Carwika Carwika

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Retrospective analysis of 300 microbial cell-free DNA sequencing results in routine blood stream infection diagnostics by Claudio Neidhöfer, Claudio Neidhöfer, Niklas Klein, Niklas Klein, Aylin Yürüktümen, Tessa Hattenhauer, Rebekka Mispelbaum, Christian Bode, Tobias A. W. Holderried, Achim Hoerauf, Marijo Parčina

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T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses by Billaud, Maud, Theodorou, Ilias, Lamy-Besnier, Quentin, Shah, Shiraz A., Lecointe, François, De Sordi, Luisa, De Paepe, Marianne, Petit, Marie-Agnès

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Protein-Protein Interactions Inferred from Domain-Domain Interactions in Genogroup II Genotype 4 Norovirus Sequences by Chuan-Ching Huang, Chuan Yi Tang

“…However, no efficient vaccine for NoVs exists because of their variable genome sequences. This study investigates the infection processes in protein-protein interactions between hosts and NoVs. …”
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Investigating intra‐tumoural heterogeneity and microenvironment diversity in primary cardiac angiosarcoma through single‐cell RNA sequencing by Jingyuan Huo, Zhen Wang, Wenting Zhao, Miao Chen, Haoyang Li, Fengpu He, Xiao Tian, Yaqi Ma, Firyuza Husanova, Liang Ma, Yiming Ni, Hongda Ding, Weidong Li, Hongfei Xu

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Transposon-sequencing across multiple Mycobacterium abscessus isolates reveals significant functional genomic diversity among strains by Chidiebere Akusobi, Sanjeevani Choudhery, Bouchra S. Benghomari, Ian D. Wolf, Shreya Singhvi, Thomas R. Ioerger, Eric J. Rubin

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Exploring Core Genes Associated with Sepsis and Systemic Inflammatory Response Syndrome Using Single-Cell Sequencing Technology by Shen Y, Leng L, Hu Y

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