Species delimitation and microalgal cryptic diversity analysis of the genus Micractinium (Chlorophyta) by E. S. Krivina, A. D. Temraleeva, Yu. S. Bukin

“…The genetic distances analysis of 18S–ITS1–5.8S–ITS2 nucleotide sequences showed that intraspecific differences in the genus ranged from 0 to 0.5 % and interspecific differences, from 0.6 to 4.7 %. …”
Get full text

Frameshift variation in the HMG-CoA reductase gene and unresponsiveness to cholesterol-lowering drugs in type 2 diabetes mellitus patients by Esmat Khaleqsefat, Khder Hussein Rasul, Ramiar Kamal Kheder, Sonia Baban, Jamil Baban

“…This mutation altered the amino acid sequence in the flap domain, replacing isoleucine with a stop codon. …”
Get full text

Intra-Articular Injection of Human Bone Marrow–Derived Mesenchymal Stem Cells in Knee Osteoarthritis: A Randomized, Double-Blind, Controlled Trial by Bong-Woo Lee, Jennifer Jooha Lee, Joon-Yong Jung, Ji Hyeon Ju

“…Magnetic resonance imaging (MRI) with T2 mapping sequences was conducted for knee cartilage assessment at baseline and at 3 and 12 months. …”
Get full text

Depletion of key gut bacteria predicts disrupted bile acid metabolism in inflammatory bowel disease by Daniel Peterson, Christopher Weidenmaier, Sonia Timberlake, Rotem Gura Sadovsky

“…BA-transformation activity mediated by the bile acid inducible (bai) operon has been functionally characterized in the genus Clostridium, and homologous bai gene sequences have been found in metagenome-assembled genomes (MAGs) belonging to other taxa in the human gut, but it is unclear which species of bai-carrying bacteria perform physiologically significant amounts of bile acid transformation in healthy and sick individuals. …”
Get full text

mRNA technology as one of the promising platforms for the SARS-CoV-2 vaccine development by A. A. Ilyichev, L. A. Orlova, S. V. Sharabrin, L. I. Karpenko

“…After the genome sequence of SARS-CoV-2 (Severe acute respiratory syndrome-related coronavirus 2) was published and the number of infected people began to increase rapidly, many global companies began to develop a vaccine. …”
Get full text

Development of RT-PCR Assays for Simple Detection and Identification of Sabin Virus Contaminants in the Novel Oral Poliovirus Vaccines by Olga Singh, Hasmik Manukyan, Erman Tritama, Shwu-Maan Lee, Jerry P. Weir, Majid Laassri

“…The nOPV vaccines have a high degree of sequence homology with the parental Sabin 2 genome, and some manufacturing facilities produce and store both Sabin OPV and nOPV. …”
Get full text

Advancing skeleton-based human behavior recognition: multi-stream fusion spatiotemporal graph convolutional networks by Fenglin Liu, Chenyu Wang, Zhiqiang Tian, Shaoyi Du, Wei Zeng

“…The present study introduces an innovative human body behavior recognition framework anchored in skeleton sequences and multi-stream fused spatiotemporal graph convolutional networks. …”
Get full text

Identification of genome compositions in allopolyploid species of the genus Elymus(Poaceae: Triticeae) in the Asian part of Russia by CAPS analysis by A. V. Agafonov, E. V. Shabanova (Kobozeva), S. V. Asbaganov, A. V. Mglinets, V. S. Bogdanova

“…On the basis of nucleotide sequences from public  databases, TaqI restrictase was selected, as it produced patterns of restriction fragments specific for St, H, and Y  haplomes easily recognizable in agarose gel. …”
Get full text

From physical activity patterns to cognitive status: development and validation of novel digital biomarkers for cognitive assessment in older adults by Ling-Jie Fan, Feng-Yi Wang, Jun-Han Zhao, Jun-Jie Zhang, Yang-An Li, Jia Tang, Tao Lin, Quan Wei

“…Methods We analyzed PA data from 3,363 older adults (NHATS: n = 747; NHANES: n = 2,616), with each participant contributing a complete 3-day continuous activity sequence. We extracted the most relevant PA features associated with cognitive function using feature engineering and recursive feature elimination. …”
Get full text

EFFECTIVENESS OF THE REHABILITATION APPROACH BASED ON THE VISUAL MODEL OF MOBIUS TAPE IN THE TREATMENT OF MYOFASCIAL PAIN SYNDROME OF THE CERVICAL REGION IN COMBATANTS by Ye. Yu. Strashko

“…In particular, it involves low cost and ease of implementation, and it should be noted that the comprehensive, at first glance, rehabilitation process is simplified due to mathematical logic, sequence, creativity, and game form.…”
Get full text

An optimized deep-forest algorithm using a modified differential evolution optimization algorithm: A case of host-pathogen protein-protein interaction prediction by Jerry Emmanuel, Itunuoluwa Isewon, Jelili Oyelade

“…The performance of the optimized deep forest model was evaluated on human-Plasmodium falciparum protein sequence datasets using 10-fold cross-validation. The results were compared with standard optimization methods such as traditional Bayesian optimization, genetic algorithms, evolutionary strategies, and other machine learning models. …”
Get full text

Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009 by Xiaoyue Shen, Wenkui Sun, Yi Shi, Zheng Xing, Xin Su

“…In this study, we employed reverse genetics approach to generate a recombinant H1N1pdm with a cell-specific miRNA target sequence inserted into its PB1 genomic segment to investigate whether miRNAs are able to suppress H1N1pdm replication. …”
Get full text

THE DEVELOPMENT OF A MOBILE APPLICATION ISMIND FOR FORMATIVE STUDENTS’ ASSESSMENT by Aslina Saad, Fatin Naiemah Khairulanuar, Bahbibi Rahmatullah

“…Various CASE tools which accelerated the development process in various stages of software development were explained.UMLet software was employed to create a use case diagram, activity diagram, and sequence diagram. The user interface design, which followed The Eight Golden Rules of Interface, was developed utilizing Marvel software. …”
Get full text

Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer by Christian Arstad, Kristin Taskén, Paulo Refinetti, Ulrika Axcrona, Karl-Erik Giercksky, Per Olaf Ekstrøm

“…From a total number of 1728 microsamples, mitochondrial DNA target sequences were amplified and base substitutions detected by cycling temperature capillary electrophoresis. …”
Get full text

A Study on the Spatiotemporal Heterogeneity and Driving Forces of Ecological Resilience in the Economic Belt on the Northern Slope of the Tianshan Mountains by Keqi Li, Qingwu Yan, Zihao Wu, Guie Li, Minghao Yi, Xiaosong Ma

“…Firstly, a sub-item evaluation of ecological resilience was conducted from three aspects, including ecological vulnerability evaluation based on the CRITIC and AHP models, ecological health evaluation based on the InVEST model, and landscape connectivity evaluation based on the MSPA method. Then, the sequence polygon method was utilized to conduct a comprehensive multi-dimensional assessment of ecological resilience based on the aforementioned three evaluation results. …”
Get full text

Newly isolated halotolerant Gordonia terrae S-LD serves as a microbial cell factory for the bioconversion of used soybean oil into polyhydroxybutyrate by Song Xu, Ruiqin Han, Lidan Tao, Zhipeng Zhang, Junfei Gao, Xinyuan Wang, Wei Zhao, Xiaoxia Zhang, Zhiyong Huang

“…In addition, the whole genome was sequenced, and the PHB biosynthetic pathway and quantitative expression of key genes were delineated in the novel isolated strain. …”
Get full text

Identification of species in the genus Nitraria L. (Nitrariaceae) based on nucleotide variability of nuclear ribosomal DNA by T. A. Poliakova, E. V. Banaev, M. A. Tomoshevich

“…Intragenomic polymorphism of ITS1 and ITS2 of nuclear ribosomal DNA sequences was analysed in 33 samples belonging to the Nitraria species N. schoberi, N. sibirica, and N. komarovii. …”
Get full text

HAD-YOLO: An Accurate and Effective Weed Detection Model Based on Improved YOLOV5 Network by Long Deng, Zhonghua Miao, Xueguan Zhao, Shuo Yang, Yuanyuan Gao, Changyuan Zhai, Chunjiang Zhao

“…With the purpose of improving the model’s capacity to extract features and making it more lightweight, this algorithm employs the HGNetV2 as its backbone network. The Scale Sequence Feature Fusion Module (SSFF) and Triple Feature Encoding Module (TFE) from the ASF-YOLO are introduced to improve the model’s capacity to extract features across various scales, and on this basis, to improve the model’s capacity to detect small targets, a P2 feature layer is included. …”
Get full text

Molecular mechanisms and comparative transcriptomics of diapause in two corn rootworm species (Diabrotica spp.) by Melise C. Lecheta, Chad Nielson, B. Wade French, Emily A.W. Nadeau, Nicholas M. Teets

“…Rootworms undergo an obligate embryonic diapause to synchronize their life cycles with host plants, and we sequenced transcriptomes from both species at five time points (pre-diapause, diapause initiation, diapause maintenance, diapause termination, and post-diapause). …”
Get full text

Performance of some civil engineering structures on Odukpani clay shale, Southeastern Nigeria by Abidemi Olujide Ilori, Anthony Michael George, Ini Christopher Umoh, Ediomo O. Eyakndue, Ubong Udoette Ukpekpe

“…Whereas at depth, the same soil sequence but stable as permeability to water is very low, hence the stability of bridge foundations in them.…”
Get full text