Subtle genomic differences in Klebsiella pneumoniae sensu stricto isolates indicate host adaptation by Bridget O'Brien, Alla Yushchenko, Jinha Suh, Dongyun Jung, Zhangbin Cai, Ngoc Sang Nguyen, Makeda Semret, Simon Dufour, Séamus Fanning, Jennifer Ronholm

“…A total of 128 KpI strains (bovine n = 65; human n = 63) were whole genome sequenced and human and bovine strains were compared based on phylogenomics, the pangenome, mobile genetic elements, and differential gene abundance. …”
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Degenerated vision, altered lipid metabolism, and expanded chemoreceptor repertoires enable Lindaspio polybranchiata to thrive in deep-sea cold seeps by Yujie Yan, Inge Seim, Yang Guo, Xupeng Chi, Zhaoshan Zhong, Dantong Wang, Mengna Li, Haining Wang, Huan Zhang, Minxiao Wang, Chaolun Li

“…Abstract Background Lindaspio polybranchiata, a member of the Spionidae family, has been reported at the Lingshui Cold Seep, where it formed a dense population around this nascent methane vent. We sequenced and assembled the genome of L. polybranchiata and performed comparative genomic analyses to investigate the genetic basis of adaptation to the deep sea. …”
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Coupling ICESat-2 and Sentinel-2 data for inversion of mangrove tidal flat to predict future distribution pattern of mangroves by Xiaoyong Ming, Yichao Tian, Qiang Zhang, Yali Zhang, Jin Tao, Junliang Lin

“…We use the inundation frequency derived from the long-term Sentinel-2 image sequences and elevation data extracted from the Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2) to establish a specific relationship between inundation frequency and ground elevation using both classical and generalized regression models, a mangrove tidal flat topography covering 76.9 km2 was generated. …”
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The Arizona Molecular ISM Survey with the SMT: Variations in the CO(2–1)/CO(1–0) Line Ratio across the Galaxy Population by Ryan P. Keenan, Daniel P. Marrone, Garrett K. Keating

“…We find strong trends between r _21 and SFR, SFR surface density, star formation efficiency, and distance from the star formation main sequence (SFMS). We show that the assumption of a constant r _21 can introduce biases into the molecular gas trends in galaxy population studies and demonstrate how this affects the recovery of important galaxy scaling relations, including the Kennicutt–Schmidt law and the relation between SFMS offset and star formation efficiency. …”
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Modeling of Measuring Transducers for Relay Protection Systems of Electrical Installations by Iliya Iliev, Andrey Kryukov, Konstantin Suslov, Nikolay Kodolov, Aleksandr Kryukov, Ivan Beloev, Yulia Valeeva

“…Traditionally, these calculations are based on symmetrical components, which require the building of equivalent circuits of various sequences. This approach can lead to errors both when identifying the operating modes and when modeling the RPA devices. …”
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Infectivity in full-term placenta of Zika viruses with different lipid profiles by Eva Mazzetto, Alessio Bortolami, Davide Bovo, Matteo Stocchero, Elisa Mazzacan, Alessandra Napolitan, Valentina Panzarin, Maria Rosa Tran, Gianpiero Zamperin, Adelaide Milani, Andrea Fortin, Michela Bigolaro, Paola Pirillo, Matteo Pagliari, Claudia Zanardello, Giuseppe Giordano, Maria Teresa Gervasi, Eugenio Baraldi, Calogero Terregino, Carlo Giaquinto, Francesco Bonfante

“…We found that the two viral stocks displayed identical consensus sequences with homogeneous quasispecies composition. …”
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Multi-channel spatio-temporal graph attention contrastive network for brain disease diagnosis by Chaojun Li, Kai Ma, Shengrong Li, Xiangshui Meng, Ran Wang, Daoqiang Zhang, Qi Zhu

“…However, most existing researches tend to focus on isolated brain network sequence segmented by sliding windows, and they are difficult to effectively uncover the higher-order spatio-temporal topological pattern in DBNs. …”
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Intravoxel incoherent motion (IVIM) MRI-derived masseter muscle characteristics: a new diagnostic marker in head and neck cancer? by Kai-Lun Cheng, Hsueh-Ju Lu, Chao-Yu Shen, Chia-Wei Lin, Hui-Yu Wang, Ying-Hsiang Chou, Yeu-Sheng Tyan, Ping-Huei Tsai

“…IVIM scans, incorporating six b-values in three orthogonal directions, were conducted using a readout-segmented echo-planar sequence. The diffusion coefficient (D), perfusion fraction (f), and pseudodiffusion coefficient (D*) were derived to assess the diffusion and perfusion alterations in the masseter muscles and HNC lesions. …”
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Systemic Therapy of Neuroendocrine Neoplasia: Single Center Experience from a Cohort of 110 Consecutive Cases by Karin Mayer, Selina Kiry, Anna Yordanova, Hojjat Ahmadzadehfar, Florian C. Gaertner, Ralph A. Bundschuh, Markus Essler, Maria A. Gonzalez-Carmona, Christian P. Strassburg, Hanno Matthaei, Philipp Lingohr, Savita Bisht, Peter Brossart, Georg Feldmann

“…Treatment of NEN patients remains challenging due to lack of prospective evidence on the choice of ideal therapeutic sequence and therapeutic efficacy in specific individual scenarios. …”
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Unelongated Stems are an Active Nitrogen-Fixing Site in Rice Stems Supported by Both Sugar and Methane Under Low Nitrogen Conditions by Takanori Okamoto, Yukina Hotta, Rina Shinjo, Yoko Masuda, Arisa Nishihara, Ryosuke Sasaki, Masami Yokota Hirai, Reo Nishiwaki, Sota Miyado, Daisuke Sugiura, Motohiko Kondo

“…N fixation, estimated using the acetylene reduction assay, was also higher in the leaf sheath and root than in the inner part of the unelongated stem and culm. Amplicon sequence variants (ASVs) close to sugar-utilizing heterotrophic diazotrophs belonging to Betaproteobacteria and type II methanotrophic diazotrophs belonging to Alphaproteobacteria were abundant in the outer part of the unelongated stems. …”
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Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma by Yujiao Zhou, Feifei Liu, Chengmin Li, Guo Shi, Xiaolei Xu, Xue Luo, Yuanling Zhang, Jingjie Fu, Aizhong Zeng, Limin Chen

“…Firstly, the total AAH mRNA was extracted from human HCC tissues; the cDNA was amplified by RT-PCR, verified, and sequenced after TA cloning. Gateway technology was used and the obtained 18T-AAH was used as a substrate, to yield the final expression vector Ad-AAH-IRES2-EGFP. …”
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Analysis of expression and transport activity of sugar transporter gene HpSWEET7 from red pitaya by ZHENG Qianming, YAN Shuang, XIE Pu, WANG Honglin, MA Yuhua

“…[Methods] HpSWEET7 gene was isolated from ‘Zihonglong’, and sequence alignment and phylogenetic relationship were analyzed. …”
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Influence of human peripheral blood samples preprocessing on the quality of Hi-C libraries by M. M. Gridina, E. Vesna, M. E. Minzhenkova, N. V. Shilova, O. P. Ryzhkova, L. P. Nazarenko, E. O. Belyaeva, I. N. Lebedev, V. S. Fishman

“…For each condition, we prepared and sequenced Hi-C libraries. The quality of the obtained data was compared. …”
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Correlation Analysis between GDM and Gut Microbial Composition in Late Pregnancy by Genxia Li, Pan Yin, Shuhui Chu, Wanli Gao, Shihong Cui, Shuhua Guo, Yajuan Xu, Enwu Yuan, Texuan Zhu, Jie You, Junya Zhang, Manman Yang

“…Therefore, this study is aimed at exploring the characteristics of the intestinal flora of patients with gestational diabetes in the third trimester of pregnancy and at finding the intestinal flora with significant differences in healthy pregnant women to provide a basis for future clinical attempts of using intestinal microecological agents to treat gestational diabetes mellitus (GDM). We sequenced the V3-V4 regions of the 16S ribosomal ribonucleic acid (rRNA) gene from stool samples of 52 singleton pregnant women at >28 weeks of gestation. …”
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Advancing the understanding of Naegleria fowleri: Global epidemiology, phylogenetic analysis, and strategies to combat a deadly pathogen by Awadh Alanazi, Sonia Younas, Hasan Ejaz, Muharib Alruwaili, Yasir Alruwaili, Bi Bi Zainab Mazhari, Muhammad Atif, Kashaf Junaid

“…A phylogenetic analysis of 41 N. fowleri ITS-1, 5.8S, ITS-2 region-based sequences showed genotypic diversity, with genotypes II and III being the most prevalent in Asia, North America, and Europe. …”
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Evaluation of 16s Long Read Metabarcoding for Characterizing the Microbiome and Salmonella Contamination of Retail Poultry Meat by Zachary Tobar, Katie Y. Lee, Megan E. Gaa, Bryshal P. Moore, Xunde Li, Maurice E. Pitesky

“…However, due to greater taxonomic resolution afforded by using 16s long reads, the assay allowed alpha diversity assessment of the microbiome of raw poultry meat with the ability to assign taxonomy to the species and strain level for some amplicon sequence variants (ASV). This indicates this process may have value characterizing biodiversity and pathogen contamination of poultry samples in earlier steps of the poultry meat production process where bacterial contamination concentrations are likely to be higher.…”
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Plastome data provides new insights into population differentiation and evolution of Ginkgo in the Sichuan Basin of China by Liyun Nie, Fangling Liu, Meixia Wang, Zhuying Jiang, Jiali Kong, Luke R. Tembrock, Shenglong Kan, Penghao Wang, Jie Wang, Zhiqiang Wu, Xiaoli Liu

“…Results Analysis of 227 complete plastomes, including 81 newly sampled individuals from the Sichuan Basin, revealed three distinct maternal lineages differing from known nuclear genome patterns. We identified 170 sequence variants and extensive RNA editing (235 sites) with a bias toward hydrophobic amino acid conversions, suggesting active molecular evolution. …”
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Engineered ipilimumab variants that bind human and mouse CTLA-4 by Brett Robison, SJ Diong, Anusha Kumar, Thomas M. Moon, Olin Chang, Bryant Chau, Christine Bee, Ishita Barman, Arvind Rajpal, Alan J. Korman, Sean West, Pavel Strop, Peter S. Lee

“…Identification of antibody therapeutic candidates that bind their human targets and cross-react to mouse orthologs is often challenging, especially for targets with low sequence homology. In such cases, surrogate antibodies that bind mouse orthologs must be used. …”
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MR Angiography of Peripheral Arterial Stents: In Vitro Evaluation of 22 Different Stent Types by Matthias C. Burg, Alexander C. Bunck, Harald Seifarth, Boris Buerke, Harald Kugel, Volker Hesselmann, Michael Köhler, Walter Heindel, David Maintz

“…Stents were imaged at 1.5 Tesla using a T1-weighted 3D spoiled gradient-echo sequence. Image analysis was performed measuring three categories: Signal intensity in the stent lumen, lumen visibility of the stented lumen, and homogeneity of the stented lumen. …”
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Combination gemcitabine and PD-L1xCD3 bispecific T cell engager (BiTE) enhances T lymphocyte cytotoxicity against cholangiocarcinoma cells by Methi Wathikthinnakon, Piriya Luangwattananun, Nunghathai Sawasdee, Chutipa Chiawpanit, Vannajan Sanghiran Lee, Piyarat Nimmanpipug, Yingmanee Tragoolpua, Siriphorn Rotarayanont, Thanich Sangsuwannukul, Nattaporn Phanthaphol, Yupanun Wutti-in, Chalermchai Somboonpatarakun, Thaweesak Chieochansin, Mutita Junking, Jatuporn Sujjitjoon, Pa-thai Yenchitsomanus, Aussara Panya

“…Two recombinant PD-L1xCD3 BiTEs (mBiTE and sBiTE contain anti-PD-L1 scFv region from atezolizumab and from a published sequence, respectively) were able to specifically bind to both CD3 on T lymphocytes, and to PD-L1 overexpressed after gemcitabine treatment on CCA (KKU213A, KKU055, and KKU100) cells. mBiTE and sBiTE significantly enhanced T lymphocyte cytotoxicity against CCA cells, especially after gemcitabine treatment, and their magnitudes of cytotoxicity were positively associated with the levels of PD-L1 expression. …”
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