Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells by Bernardo Bonilauri, Amanda C. Camillo-Andrade, Marlon D. M. Santos, Juliana de S. da G. Fischer, Paulo C. Carvalho, Bruno Dallagiovanna

“…We also used our previous next-generation sequencing data (RNA-seq) of the total and polysomal mRNA fractions of hASC to study posttranscriptional regulation during the initial steps of adipogenesis. …”
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Executive functions in adults born small for gestational age at term: a prospective cohort study by Mariell Nordgård, Martine Reitan Udnæs, Kristina Anna Djupvik Aakvik, Silje Dahl Benum, Sigrid Hegna Ingvaldsen, Siri Weider, Kari Anne I. Evensen

“…The SGA group used 1.6 (95% confidence interval [CI] 0.1–3.1) to 3.9 (95% CI 1.2–6.5) seconds more on TMT conditions 1 and 3, indicating more difficulties with visual scanning and letter sequencing than controls. On BRIEF-A, the SGA group scored 1.5 (95% CI 0.2–2.8) points lower on the clinical scale Emotional Control. …”
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Transcription factor KLF2 is associated with the dysfunctional status of NK cells and the prognosis of pediatric B-ALL patients by Fang Wu, Huimin Xu, Benshan Zhang

“…We also analyzed published single-cell RNA sequencing (scRNAseq) data from B-ALL and normal bone marrow samples using unsupervised clustering. …”
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hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation by Weijiang Liu, Fulin Yuan, Haitao Bai, Yuanlin Liu, Xue Li, Yang Wang, Yi Zhang

“…To reveal their potential immunosuppressive molecules, we used RNA sequencing to analyze gene expression in different tissue-derived MSCs, including human bone marrow, umbilical cord, amniotic membrane, and placenta, and found that chitinase-3-like protein 1 (Chi3l1) was highly expressed in human umbilical cord mesenchymal stem cells (hUC-MSCs). …”
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Isolation and Identification of Some Probiotic Bacteria and Their Potential Role in Improving Immune Response and Resistance of Nile Tilapia (Oreochromis niloticus) in Comparison w... by Mosleh M. Abomughaid

“…The potential probiotic based on the in vitro antimicrobial activity test was identified as P. putida using routine and gel electrophoresis and 16S rRNA sequencing. During the first and the second month of experiment, there was a highly significant increase in the survival percent of the experimental fish in both treated groups with probiotics. …”
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Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing fact... by Ilkay Celik Gulsoy, Terrens N V Saaki, Michaela Wenzel, Simon Syvertsson, Taku Morimoto, Tjalling K Siersma, Leendert W Hamoen

“…Viability of the multiple deletion mutant was not greatly affected, although the frequency of cell division was considerably reduced. Whole genome sequencing suggested that the construction of this minimal divisome strain was also possible due to the accumulation of suppressor mutations. …”
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Chromosome‐scale genome assembly of Poa trivialis and population genomics reveal widespread gene flow in a cool‐season grass seed production system by Caio A. C. G. Brunharo, Christopher W. Benson, David R. Huff, Jesse R. Lasky

“…A reduced‐representation sequencing analysis of field populations revealed limited genetic diversity and suggested potential gene flow and human‐assisted dispersal in the region. …”
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Cystic Echinococcosis/Hydatid Cyst Coinfection with HIV: A Report from Shiraz, Iran by Yosef Sharifi, Seyed Mahmoud Sadjjadi, Hamed Nikoupour Dailami, Seyed Hamed Jafari, Mohammad Hossein Anbardar, Mohammad Bagher Khosravi

“…The IgG ELISA test with native AgB for E. granulosus infection was also positive. mtDNA amplification using PCR and sequencing showed the cyst as E. granulosus sensu stricto genotype. …”
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Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure by L. A. Yarinich, A. A. Ogienko, A. V. Pindyurin, E. S. Omelina

“…Next, using the high-throughput sequencing, genomic localizations of barcodes, as well as their abundances in the population and transcriptional activities were identified. …”
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Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure by Juan Hu, Yurui Zeng, Aibin Hu, Xiaofeng Wang

“…In this study, the chemical structure of CM digestate-derived DOM was characterized by using multi-spectroscopic techniques and ultrahigh-resolution mass spectrometry, and the microbial composition was detected by using 16S rRNA gene sequencing. The results revealed that the DOM contained abundant free amino acids and protein-like compounds but fewer humic-like substances, identified as lignin/carboxylate-rich alicyclic molecules, lipids, and proteins/aliphatic compounds featuring enriched S_5–6O₁ and N_1–5O_X fragments. …”
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Correlation between dominant bacterial community and non-volatile organic compounds during the fermentation of shrimp sauces by Ruichang Gao, Huijie Liu, Ying Li, Hongying Liu, Yue Zhou, Li Yuan

“…Moreover, the dynamics of microbial diversity during processing was investigated by using high-throughput sequencing technology. The results showed that the AAN, TVB-N, organic acid, 5'-nucleotide and FAA content were in range of 0.93–1.42 g/100mL, 49.91–236.27 mg/100mL, 6.65–20.68 mg/mL, 3.51–6.56 mg/mL and 81.27–102.90 mg/mL. …”
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Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants by Samia Hamane, Anass El Yemlahi, Mounir Hassani Zerrouk, Ouiam El Galiou, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

“…Based on 16S rRNA gene sequencing, the strains were identified as Enterobacter and Serratia. …”
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Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors by Catia Pilon, Andrea Rebellato, Riccardo Urbanet, Vincenza Guzzardo, Rocco Cappellesso, Hironobu Sasano, Ambrogio Fassina, Francesco Fallo

“…Methylation of CpG-rich 5′ regions was assessed by bisulfite sequencing PCR using bisulfite-treated DNA from archival microdissected paraffin-embedded adrenocortical tissues. …”
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Evaluation of common COL2A1 gene variants in Iranian patients suspected with Stickler syndrome type I by Fatemeh Abolhasani, Hossein Abdali, Mohammad Kazemi, Bijan Movahedian Attar, Fatemeh Derakhshandeh, Majid Hosseinzadeh

“…Subsequently, the purified amplicons were subjected to Sanger sequencing to identify common variants associated with Stickler syndrome type I. …”
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Molecular genetic basis of epidermolysis bullosa by Yu. Yu. Kotalevskaya, V. A. Stepanov

“…In general, the study of clinical, genetic and ultrastructural changes in EB has significantly expanded the understanding of the natural history of the disease and supplemented the data on genotype-phenotype correlations, promotes the search and study of epigenetic and non-genetic disease modifier factors, and also allows developing approaches to radical treatment of the disease. New advances of sequencing technologies have made it possible to describe new phenotypes and study their genetic and molecular mechanisms. …”
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Machine Learning Enabled Prediction of Biologically Relevant Gene Expression Using CT‐Based Radiomic Features in Non‐Small Cell Lung Cancer by Shrey S. Sukhadia, Christoph Sadée, Olivier Gevaert, Shivashankar H. Nagaraj

“…Methods In a retrospective study of two NSCLC patient cohorts separated by 5 years, we performed a radiogenomic analysis of previously disseminated data from 2018 (n = 116) and newly acquired data from 2023 (n = 44) using RNA sequencing and lung CT images. Combining the data from two cohorts post binarization (of gene expression) or batch normalization (of radiomic features) in each cohort proved to be a better approach as compared to training the model on one cohort and validating on the other. …”
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Molecular Evidence of Emerged Pulmonary Lophomoniasis due to Lophomonas blattarum among Hospitalized Patients in Southwestern Iran: A National Registry-Based Study by Kobra Mokhtarian, Simin Taghipour, Maryam Nakhaei, Amirmasoud Taheri, Ali Sharifpour, Mahdi Fakhar, Hajar Ziaei Hezarjaribi

“…A total of 132 frozen BALF specimens were examined, 36 (27.3%) tested Lophomonas spp. positive using the conventional PCR technique. Also, based on sequencing data and blast analysis, the presence of L. blattarum species was confirmed. …”
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Exosomes derived from liver failure patients’ plasma stimulated mesenchymal stem cells alleviate acute liver failure by Zhuoran Wang, Jun Ling, Shaoli You, Bing Zhu

“…Methods Untreated exosomes (NC-Exos) and LF-Exos were extracted and characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), western blotting, and miRNA sequencing. We then examined the protective effects of LF-Exos on hepatocytes acutely injured by D-galactosamine (D-GalN)/lipopolysaccharide (LPS) co-treatment and on a mouse model of acute liver failure (ALF). …”
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Vegetation types shape the soil micro-food web compositions and soil multifunctionality in Loess Plateau by Zhiming Chen, Wenjuan Kang, Renyuan He, Guang Li, Zhuzhu Luo, Zhuzhu Luo

“…., and the control vegetation Stipa bungeana on soil micro-food webs and soil multifunctionality, as well as their response mechanisms to soil environmental drivers, were investigated using High-throughput sequencing technology.ResultsC. korshinskii significantly enhanced soil physicochemical properties and soil enzyme activities by facilitating the stability of the soil micro-food web structure driven by soil bacteria and fungi and increasing the soil multifunctionality in contrast to S. bungeana. …”
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Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli by Jie Fang, Xiaojiao Han, Lihua Xie, Mingying Liu, Guirong Qiao, Jing Jiang, Renying Zhuo

“…Here, a full-length cDNA library of A. glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis. Among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and transport, or regulation of transcription. …”
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