Elevated ALOX12 in renal tissue predicts progression in diabetic kidney disease by Meixi Wang, Jingjing Wang, Jinni Wang, Yonggui Wu, Xiangming Qi

“…The present study sequenced db/m mouse and db/db mouse to determine the ALOX12 genetic changes related to DKD. …”
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Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya by Robert O. Okayo, Darius O. Andika, Mathews M. Dida, George O. K’Otuto, Bernard M. Gichimu

“…Molecular characterization was done through amplification and comparison of the partial sequence of the ITS1-5.8S-ITS2 region. The expression analysis of aflR, aflS, aflD, aflP, and aflQ genes in the aflatoxin biosynthesis pathways was conducted to confirm the positive identification of A. flavus. …”
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Case report: The value of early application of mNGS technology in the diagnosis and treatment of severe Legionnaires’ disease: reports of two cases with different outcomes by Jianqing Fang, Zhe Wang, Yu Shen, Xuenong Wu, Hao Fang, Xiaokui Sun, Ting Yu, Qingqing Zhang

“…Increasing evidence shows that metagenomic next-generation sequencing (mNGS) has excellent potential for the early identification of pathogens. …”
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Thrips dynamics in Allium crops: Unraveling the role of reproductive mode and weather variables in Thrips tabaci population development. by Bettina Porta, Ben Vosman, Pablo González Barrios, Richard G F Visser, Guillermo A Galván, Olga E Scholten

“…The thrips populations from each location studied were characterized by sequencing 33 individuals per sampling using the cytochrome oxidase subunit I (COI) gene. …”
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Melanophilin-induced primary cilia promote pancreatic cancer metastasis by Yu-Ying Chao, Ruei-Ci Lin, Ping-Jui Su, Chu-An Wang, Ting-Yuan Tu, Ya-Chin Hou, Yi-Tzui Tsai, I-Chen Peng, Shaw-Jenq Tsai, Yan-Shen Shan, Chia-Yih Wang

“…Here, we demonstrated that melanophilin (MLPH) promotes PDAC metastasis by inducing the regrowth of primary cilia. Using RNA sequencing, we found that MLPH was upregulated in Gln-deficient conditions. …”
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The Combination of Host-Associated Bacillus megaterium R32 and Stachyose Promotes the Intestinal Health of Turbot (Scophthalmus maximus. L) by Yaoyao Kong, Sifan Zhao, Weihao Ou, Kangsen Mai, Yanjiao Zhang

“…The results showed that diets RS and RC significantly inhibited the expression of cell development and apoptosis-related genes (β-catenin, CyclinD1, BAX, Bid); diets RS and RC significantly increased the expression of intestinal tight junction protein claudin-4, while RS group significantly decreased the expression of myosin light chain kinase; diets RS and RC significantly decreased the expression of proinflammatory factors (IL-13, IL-15, IFN- γ), diet RS also significantly decreased the expression of TNF-α and AP-1, and increased the expression of TGF-β. 16s rRNA gene sequencing results showed that diets RS and RC significantly decreased the abundance of conditional pathogenic bacteria (Corynebacterium, Desulfovibrio, Escherichia-Shigella). …”
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Clinical considerations on antimicrobial resistance potential of complex microbiological samples by Norbert Solymosi, Adrienn Gréta Tóth, Sára Ágnes Nagy, István Csabai, Csongor Feczkó, Tamás Reibling, Tibor Németh

“…Next and third generation sequencing technologies allow the identification of antimicrobial resistance genes (ARGs) present in a bacterial community. …”
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Effects of continuous cropping on bacterial community diversity and soil metabolites in soybean roots by Liwei An, Xinnan Lu, Pengyu Zhang, Jiayao Sun, Baiming Cong, Rula Sa, Dexin He

“…This study employed both field and pot positioning experiments, using high-throughput and generic metabolomics sequencing techniques to explore the dynamics between soybean plants, root microflora, and soil metabolites, especially in the context of continuous cropping and fluctuating corn–soybean crop rotation. …”
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Liver macrophage-derived exosomal miRNA-342-3p promotes liver fibrosis by inhibiting HPCAL1 in stellate cells by Wenshuai Li, Lirong Chen, Qi Zhou, Tiansheng Huang, Wanwei Zheng, Feifei Luo, Zhong Guang Luo, Jun Zhang, Jie Liu

“…Method Exosomes were extracted from primary mouse macrophages and cultured with HSCs, and the differential expression of microRNAs was evaluated using high-throughput sequencing technology. The functions of miR-342-3p in exosomes were verified by qPCR and luciferase reporter gene experiments with HSCs. …”
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Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptatio... by Sergio Stefanni, Raul Bettencourt, Miguel Pinheiro, Gianluca De Moro, Lucia Bongiorni, Alberto Pallavicini

“…We carried out a high throughput sequencing analysis on a 454 GS-FLX titanium plate using unnormalized cDNA libraries from six tissues of A. carbo. …”
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Canagliflozin attenuates kidney injury, gut-derived toxins, and gut microbiota imbalance in high-salt diet-fed Dahl salt-sensitive rats by Lili He, Qingjuan Zuo, Sai Ma, Guorui Zhang, Zhongli Wang, Tingting Zhang, Jianlong Zhai, Yifang Guo

“…This study employed 16S rRNA sequencing technology and liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based metabolic profiling combined with advanced differential and association analyses to investigate the correlation between the microbiome and the metabolome in male DSS rats.Results A high-salt diet disrupted the balance of the intestinal flora and increased toxic metabolites (methyhistidines, creatinine, homocitrulline, and indoxyl sulfate), resulting in severe kidney damage. …”
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Integration of transcriptomics and metabolomics data revealed role of insulin resistant SNW1 gene in the pathophysiology of gestational diabetes by Ravi Bhushan, Rimjhim Trivedi, Ritu Raj, Anjali Rani, Sangeeta Rai, Anima Tripathi, Shiv Govind Rawat, Ajay Kumar, Dinesh Kumar, Pawan K. Dubey

“…High-throughput RNA sequencing and computational bioinformatics analyses were conducted to identify novel hub genes, and their regulatory mechanisms were validated through qRT-PCR, western blot, and siRNA-mediated knockdown studies. …”
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Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7 by Changjiao Luan, Yue Gao, Jun Zhao, Xiaohui Zhang, Chaofan Wang, Wentao Sun, Yucheng Li, Xinxing Yang, Jiaxiao Chen, Weili Liu, Weijuan Gong, Xingjie Ma

“…In this study, we conducted RNA sequencing on bleomycin-induced senescent lung tissues from mice and identified Clic3 as the most significantly upregulated CLIC member. …”
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The Single-Nucleotide Polymorphism (SNP) Validity to Detect Omicron Variants by Lia Gardenia Partakusuma, Luhung Budiailmiawan, null Budiman, Ida Parwati, null Aryati, Basti Andriyoko, Louisa Markus, Corine Niswara, Cut Nur Cinthia Alamanda

“…Variants of SARS-CoV-2 are determined by the whole genome sequencing (WGS) assay, which is time-consuming, with limited availability (only in several laboratories). …”
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Pre-therapeutic efficacy of the CDK inhibitor dinaciclib in medulloblastoma cells by Marta Buzzetti, Sonia Morlando, Dimitrios Solomos, Ammara Mehmood, Alexander W. I. Cox, Mattia Chiesa, Yuri D’Alessandra, Michela Garofalo, Caroline H. Topham, Gianpiero Di Leva

“…We present evidence supporting dinaciclib’s ability to inhibit MB cells in vitro proliferation at considerably lower doses than palbociclib. Sequencing data and pathway analysis suggested that dinaciclib is a potent cell death inducer in MB cells. …”
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Combined PARP14 inhibition and PD-1 blockade promotes cytotoxic T cell quiescence and modulates macrophage polarization in relapsed melanoma by Chun Wai Wong, Rotem Leshem, Kieran Neil Sefton, I-Hsuan Lin, Dervla Tamara Isaac, Mario Niepel, Adam Hurlstone

“…Using preclinical models and single-cell RNA sequencing, we investigated immune and tumor cell adaptations to PARP14 inhibition combined with PD-1 blockade.Results Combining PARP14 inhibition and PD-1 blockade suppressed tumor-associated macrophages while increasing proinflammatory memory macrophages. …”
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Mutant Prevention Concentration of Ciprofloxacin against Klebsiella pneumoniae Clinical Isolates: An Ideal Prognosticator in Treating Multidrug-Resistant Strains by B. Aditi Priyadarshini, Krishnan Mahalakshmi, Venkatesan Naveen Kumar

“…The detection of qnr genes for 24 isolates and DNA sequencing for six isolates were performed. Results. …”
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Microbiome analysis in individuals with human papillomavirus oral infection by David Israel Escobar Marcillo, Grete Francesca Privitera, Francesca Rollo, Alessandra Latini, Eugenia Giuliani, Maria Benevolo, Massimo Giuliani, Barbara Pichi, Raul Pellini, Maria Gabriella Donà

“…DNA purified from oral rinse-and-gargles of HIV-infected (HIV+) and HIV-uninfected (HIV−) individuals were used for 16S rRNA gene V3–V4 region amplification and sequencing. Analysis of differential microbial abundance and differential pathway abundance was performed, separately for HIV+ and HIV− individuals. …”
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Assessment of microbial diversity and antimicrobial resistance in raw camel milk: Genomic and phenotypic analysis of Kharai and Kutchi breeds of Gujarat, India by Hetvi J. Adhyaru, Devangi B. Mangroliya, Jayesh H. Kabariya, Vimal M. Ramani

“…The genotypic analysis, performed via nanopore metagenomic sequencing, revealed the presence of various antimicrobial resistance genes (ARGs). …”
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Search for differentially methylated regions in ancient and modern genomes by D. D. Borodko, S. V. Zhenilo, F. S. Sharko

“…The application of existing whole­genome bisulfite sequencing methods is limited for ancient samples due to their low quality and fragmentation, and the approach to obtaining DNA methylation profiles differs significantly between ancient hunter­gatherer samples and modern tissues. …”
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