An Approach to the Investigation of Thrombocytosis: Differentiating between Essential Thrombocythemia and Secondary Thrombocytosis by Ala Almanaseer, Benjamin Chin-Yee, Jenny Ho, Alejandro Lazo-Langner, Laila Schenkel, Pratibha Bhai, Bekim Sadikovic, Ian H. Chin-Yee, Cyrus C. Hsia

“…A practical diagnostic approach to identify secondary thrombocytosis could reduce overinvestigation such as next generation sequencing (NGS) panel. Methods and Results. All adult patients with thrombocytosis (≥450 × 109/L) who underwent molecular testing at a single tertiary care centre between January 1, 2018 and May 31, 2021 were evaluated. …”
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Comparative Genomics of the First and Complete Genome of “Actinobacillus porcitonsillarum” Supports the Novel Species Hypothesis by Valentina Donà, Vincent Perreten

“…Previous studies suggested that “A. porcitonsillarum” may represent a new species closely related to Actinobacillus minor, yet no full genome has been sequenced so far. We implemented the Oxford Nanopore and Illumina sequencing technologies to obtain the highly accurate and complete genome sequence of the “A. porcitonsillarum” strain 9953L55. …”
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Eurotium Cristatum Postfermentation of Fireweed and Apple Tree Leaf Herbal Teas by Tatiana A. Efimenko, Elena F. Shanenko, Tatiana G. Mukhamedzhanova, Olga V. Efremenkova, Yuriy A. Nikolayev, Elena N. Bilanenko, Marina V. Gernet, Artem G. Grishin, Ivan N. Serykh, Sergey V. Shevelev, Byazilya F. Vasilyeva, Svetlana N. Filippova, Galina I. El-Registan

“…The species identification was carried out based on morphocultural characteristics and DNA sequencing. This study is aimed at determining the feasibility of making postfermented herbal teas using E. cristatum and to evaluate their quality. …”
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Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance by Aditya Narayan Konwar, Surajit Basak, Shalini Gurumayum, Jagat Chandra Borah, Debajit Thakur

“…Phylogenetic analysis based on 16 S rRNA gene sequencing revealed that the isolates were predominantly from the genus Streptomyces. …”
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Correlation between Acoustic Feature and Sensory Crispness of Different Puffed Food Based on Energy Release Analysis by Chengkai ZHU, Xinnan HU, Zhili JI, Wangyang SHEN, Xiwu JIA

“…The sensory group was formed by selecting individuals adept at identifying brittleness through sequencing test, with sensory assessments conducted on various puffed food. …”
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Cucurbitacin IIa Alleviates Colitis via Promoting the Release of Host-Derived Extracellular Vesicles Encapsulating microRNA-30b-5p by Zhao Y, Jiang B, Zuo S

“…EVs were isolated and characterized, followed by analysis of the small RNAs and proteins encapsulated within them using small RNA sequencing and proteomics, respectively.Results: Our results revealed that cucurbitacin IIa alleviated colitis symptoms in DSS-treated rats, along with changes in the morphology and composition of intestinal EVs. …”
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Soil microbiome composition is highly responsive to precipitation and plant composition manipulations in a field biodiversity experiment by Haley M. Burrill, Haley M. Burrill, Susan M. Magnoli, James D. Bever

“…Most studies of climate forces and plant biodiversity effects on microbiome consider the perturbations and the microbial functional groups in isolation preventing us from understanding the full picture of the relative and differential impacts of perturbations on microbial functional groups.MethodsWe measured changes in multiple microbial communities with different functionality, including plant mutualists and pathogens, after three growing seasons in a full-factorial experiment manipulating precipitation (50%, 150% of ambient), plant diversity, and plant composition. Using amplicon sequencing to characterize the response of fungi, arbuscular mycorrhizal fungi, bacteria and oomycetes, and we found that composition of all microbial groups differentiated strongly between precipitation treatments.ResultsOomycete and bacterial diversity increased with 150% precipitation, while AM and saprotroph fungal diversity decreased. …”
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Hemophilia A Haplotypes in Southwestern Iran by Bizhen Keikhaei Dehdezi, Ali Khodadadi, Arta Farhadi Kia, Faezeh Abbasi, Saeed Bitraf, Roya Salehi Kahyesh

“…The severity of the disease was determined based on the results of the patient's factor VIII levels, and the mutation type was determined based on molecular testing and sequencing (mutation determination by direct method or gene linkage study, including VNTR). …”
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Leptospira and Inflammation by C. F. Gonçalves-de-Albuquerque, P. Burth, A. R. Silva, M. Younes-Ibrahim, H. C. Castro-Faria-Neto, M. V. Castro-Faria

“…Many molecules contribute to the ability of Leptospira to adhere, invade, and colonize. The recent sequencing of the Leptospira genome has increased our knowledge about this pathogen. …”
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Case report: Therapeutic use of bortezomib in a patient with Schnitzler syndrome by Hua Bai, Dongming Zhou, Jinwen Liu, Jie He, Zhou Min, Wenyong Fan, Bing Chen, Yong Xu

“…Laboratory tests identified the presence of IgMκ monoclonal protein, and the absence of IL-1β in serum. Whole exome sequencing (WES) did not reveal any pathological variants associated with monogenic autoinflammatory diseases or the MYD88 L265P mutation. …”
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Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine by Seyed Muhammad Hamid Malekpour, Moj Khaleghi, Alireza Akhtarpour

“…According to the 16S rRNA sequencing, the HM24 strain belonged to the genus Microbacterium with 99.97% identity. …”
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Impact of bedaquiline resistance probability on treatment decision for rifampicin-resistant TB by T.P.H. Trang, R. Kessels, T. Decroo, A. Van Rie

“…We employed mixed-effects multinomial logistic regression to quantify the effect of prBDQR, patient attributes, and contextual factors on the decision to continue BDQ or not when sequencing results become available. RESULTS: PrBDQ R was the most influential factor for BDQ decision-making, three times greater than treatment response. …”
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Temporal and Spatial eDNA Analysis of Fish Assemblages in Postindustrial, Urban Coastal Habitats by Alice Cunnington, Erika Neave, Peter Shum, Rupert Collins, Stefano Mariani

“…Results also corroborate previous findings on the importance of “molecular bycatch” (nontarget sequences) in expanding our understanding of the anthropogenic influences on the natural environment. …”
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Distribution and frequency of genetic mutations in three insecticide targets in field populations of Culex tritaeniorhynchus in Mianyang City, Sichuan Province, China by Hongwei Xie, Meilin Tang, Hongying Sun, Zhengzheng Huang, Mengmeng Dong, Xianying Wen

“…In the study, a total of 314 adults of Cx. tritaeniorhynchus, collected from 6 sites across Mianyang City, were inspected for resistance-conferring mutations in three genes encoding voltage-gated sodium channel (VGSC), Gamma-aminobutyric acid (GABA) receptor Rdl subunit (Rdl), and acetylcholinesterase (AChE) by DNA Sanger sequencing. The results showed that the classical L1014F mutation in VGSC was distributed in all the 6 populations at varying frequencies from 16.98% to 27.78%, and the frequency of F455W mutation in AChE was extremely high (97.06%-100%). …”
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Increased RTN3 phenocopies nonalcoholic fatty liver disease by inhibiting the AMPK–IDH2 pathway by Hao Huang, Shuai Guo, Ya‐Qin Chen, Yu‐Xing Liu, Jie‐Yuan Jin, Yun Liang, Liang‐Liang Fan, Rong Xiang

“…The RTN3 transgenic mice exhibited the phenotypes of fatty liver and lipid accumulation. Single‐cell RNA sequencing analysis indicated that increased RTN3 might induce mitochondrial dysfunction. …”
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eEF-2K Deficiency Boosts the Virus-Specific Effector CD8⁺ T Cell Responses During Viral Infection by Liqing Wang, Benny Shone Song, Rayansh Poojary, Xiaofang Xiong, Xingcong Ren, Jin-Ming Yang, Jianxun Song

“…We found that eEF-2K-deficient (eEF-2K⁻/⁻) mice exhibited a significantly higher proportion of VACV-specific effector CD8⁺ T cells without compromising the development of VACV-specific memory CD8⁺ T cells. RNA sequencing demonstrated that eEF-2K⁻/⁻ VACV-specific effector CD8⁺ T cells had enhanced functionality, which improves their capacity to combat viral infection during the effector phase. …”
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Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal by Anicet Georges Bruno Manga, Godar Sene, André Amakobo Diatta, Tahir Abdoulaye Diop, Gérard Barroso, Diederik van Tuinen

“…The vast majority of operational taxonomic units (OTUs) isolated in this study had no homologous sequences in the databases.…”
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Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain by Nourhan A. Zaghloul, Mona K. Gouda, Yasser Elbahloul, Nancy M. El Halfawy

“…Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active. …”
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Comparative gametogenesis and genomic signatures associated with pollen sterility in the seedless mutant of grapevine by Siddhi Chavan, Satish Phalake, Sujata Tetali, Vitthal T. Barvkar, Ravindra Patil

“…RNAseq and genome sequencing data collectively indicate parthenocarpic seedless phenotype due to aberrant developmental and physiological processes involved in pollen formation, maturation and germination in the seedless mutant of ARI 516. …”
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Differences in Rhizosphere Microbial Community Structure and Composition in Resistance and Susceptible Wheat to Fusarium Head Blight by Han Li, Mingshuang Tang, Tao Zheng, Ming Yang, Youning Wang, Yating Shuai, Yan Li, Yibo Zhang, Dongfang Ma

“…In this study, high-throughput sequencing technology was used to analyze the rhizosphere soil microbial metagenomes of 4 wheat cultivars with different levels of resistance to FHB. …”
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