Primary Signet Ring Cell/Histiocytoid Carcinoma of the Eyelid: Clinicopathologic Analysis with Evaluation of the E-Cadherin/β-Catenin Complex and Associated Genetic Alterations by Maria Del Valle Estopinal, Lavinia P. Middleton, Bita Esmaeli, Keyur P. Patel, Sara Nowroozizadeh, Michelle D. Williams

“…Immunohistochemical studies for the expression of E-cadherin, β-catenin, c-Myc, Cyclin D1, Src, and p53 were analyzed. Next generation sequencing for the detection of somatic mutations was performed on each tumor with matched normal tissue, examining 50 cancer-related genes. …”
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Clinical Features of Four West Nile Virus Cases and Its Molecular Characterization from a South Indian Tertiary Care Hospital by Shoba Mammen, Aiswarya Nair, Santhosh Kumar, Zayina Zonderveni, A. T. Prabhakar, Turaka Vijay Prakash, Sanjith Aaron, Mathew Alexander, Anand Zachariah, Asha Mary Abraham

“…West Nile virus infection was diagnosed with the available molecular techniques such as PCR and sequencing, which emphasizes the need for considering West Nile virus in the differential diagnosis of acute meningoencephalitis and the wider availability of molecular diagnostic tests.…”
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Improvements in pig agriculture through gene editing by Kristin M. Whitworth, Jonathan A. Green, Bethany K. Redel, Rodney D. Geisert, Kiho Lee, Bhanu P. Telugu, Kevin D. Wells, Randall S. Prather

“…Concordant with the developments in genomic sequencing approaches, progress among germline editing efforts is expected to reach feverish pace. …”
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Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia by Faruku Bande, Siti Suri Arshad, Latiffah Hassan, Zunita Zakaria

“…Selected samples that were FeLV-positive by PCR were subjected to sequencing, phylogenetic analysis, and motifs search. …”
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TERT-TP53 mutations: a novel biomarker pair for hepatocellular carcinoma recurrence and prognosis by Jin Li, Ling Bai, Zhaodan Xin, Jiajia Song, Hao Chen, Xingbo Song, Juan Zhou

“…In this study, we investigated the clinical implications of TERTpm, TP53m, and their co-occurrence in 50 HCC tissue samples using the next-generation sequencing (NGS) technology. We identified TERTpm (C228T) and TP53m in 16 (32%) and 24 (48%) samples, respectively. …”
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mTOR Inhibitor Everolimus Modulates Tumor Growth in Small-Cell Carcinoma of the Ovary, Hypercalcemic Type and Augments the Drug Sensitivity of Cancer Cells to Cisplatin by Kewei Zheng, Yi Gao, Jing Xu, Mingyi Kang, Ranran Chai, Guanqin Jin, Yu Kang

“…<b>Method</b>: In this study, we investigated the combined effects of everolimus and cisplatin on SCCOHT through both in vitro and in vivo experiments, complemented by RNA sequencing (RNA-seq) analyses to further elucidate the therapeutic impact. …”
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Tissue-resident immune cells: from defining characteristics to roles in diseases by Jia Li, Chu Xiao, Chunxiang Li, Jie He

“…The advancement of tissue-resolution technologies, such as single-cell sequencing and spatiotemporal omics, provides deeper insights into the cell morphology, characteristic markers, and dynamic transcriptional profiles of TRICs. …”
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The gastric microbiome altered by A4GNT deficiency in mice by Dawei Gong, Dawei Gong, Yuqiang Gao, Rui Shi, Xiaona Xu, Mengchao Yu, Shumin Zhang, Lili Wang, Quanjiang Dong

“…The aim of this study was to characterize the gastric microbiome in mice deficient for the production of α1,4-linked N-acetylglucosamine.MethodsThe gastric microbiome within A4gnt−/− mice and wild-type mice was analyzed using high-throughput sequencing of bacterial 16S rRNA.ResultsIn A4gnt−/− mice, which spontaneously develop gastric cancer, the community structure of the gastric microbiome was altered. …”
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Expression of the transcription factor encoding gene <i>StTCP23</i> in potato plants infected with the tuber spindle viroid by N. V. Mironenko, A. S. Orina, N. M. Lashina, О. S. Afanasenko

“…Diversity of RNA molecules in the PSTVd population was revealed using RT, cloning, and sequencing of the viroid. The expression of the StTCP23 gene in infected potato plants was assessed by quantitative PCR with the ef1α gene as a reference.Results. …”
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Integration of bulk and single-cell RNA-seq reveals prognostic gene signatures in patients with bladder cancer treated with immune checkpoint inhibitors by Mina Cho, Hyun Chang, Ju Han Kim

“…Bulk RNA expression data from a BC cohort (GSE176307) receiving ICI and single-cell sequencing data from patients with BC (GSE135337) were collected. …”
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Exploring the protective role of caffeine against Taraxacum-Induced ribotoxic stress mediated through autophagy and mitochondrial depolarization by Chien-Jung Lin, Shu-Ting Liu, Zih-Syuan Wu, Shih-Ming Huang, Teng-Wei Chen

“…In this study, we used the RNA integrity (RIN) value and 28S/18S ratio to confirm the integrity of experiments. Our RNA sequencing data showed that Taraxacum formosanum (T. formosanum) upregulated 893 genes and downregulated 509 genes and triggered hallmark genes of spliceosomes, TNF-α signaling via NF-κB, inflammatory response, and IL6-JAK-STAT3 signaling. …”
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Gene drive-based population suppression in the malaria vector Anopheles stephensi by Xuejiao Xu, Jingheng Chen, You Wang, Yiran Liu, Yongjie Zhang, Jie Yang, Xiaozhen Yang, Bin Chen, Zhengbo He, Jackson Champer

“…Both male and female drive heterozygotes show only moderate drive conversion, indicating that the nanos promoter has lower activity in A. stephensi than in Anopheles gambiae. By amplicon sequencing, we detect a very low level of resistance allele formation. …”
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16S rRNA metabarcoding applied to the microbiome of insect products (novel food): a comparative analysis of three reference databases by Gabriele Spatola, Alice Giusti, Laura Gasperetti, Roberta Nuvoloni, Alessandra Dalmasso, Francesco Chiesa, Andrea Armani

“… The 16S rRNA metabarcoding, based on Next-Generation Sequencing (NGS), is used to assess microbial biodiversity in various matrices, including food. …”
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A chemical genetic screen with the EXO70 inhibitor Endosidin2 uncovers potential modulators of exocytosis in Arabidopsis by Xiaohui Li, Diwen Wang, Xianglin Yin, Mingji Dai, Christopher J. Staiger, Chunhua Zhang

“…Through a whole‐genome sequencing‐based mapping strategy, 14 nonallelic es2s mutants were mapped and the candidate mutations reported here. …”
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Genome assembly of Hawaiian flower thrips Thrips hawaiiensis (Thysanoptera: Thripidae) by Xiaodi Hu, Houjun Tian, Yixin Chen, Yueqin Zheng, Hui Wei, Jian-Ping Chen, Chuan-Xi Zhang, Yong Chen, Yiyuan Li

“…The lack of a high-quality reference genome limits our understanding of the genetics of T. hawaiiensis. Here, we sequenced the genome of T. hawaiiensis using Oxford Nanopore sequencing technology, Illumina, and Hi-C technology, yielding a genome assembly of 287.59 Mb with scaffold N50 of 13.84 Mb. …”
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AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism by Yang Yang, Dan Li, Zhigang Liu, Kai Zhou, Wenxing Li, Yanqi Yang, Ruifang Sun, Yulong Li

“…Methods We performed bioinformatics analysis, CCK-8 assay, RNA sequencing, RT-PCR and Western blot to analyze the function and mechanism of AURKB in ccRCC. …”
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Coupling of alternative splicing and alternative polyadenylation by Zhang Xueying, Liu Feiyan, Zhou Yu

“…Finally, we discuss how long-read sequencing technologies aid in understanding the coordination of AS-APA events and envision that these findings may potentially promote the development of new strategies for disease diagnosis and treatment.…”
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Sargassum fusiforme and its fucoidan alleviates high-fat diet induced obesity associated with the improvement of the gut microbiota profile by Xiaoyu Ling, Zhengyang Li, Ning Su, Lingqing Kong, Shiwei Han, Dafeng Song

“…This study investigates the mechanisms by exploring changes in gut microbiota composition, gene expression, and metabolites in mice fed a high-fat diet after intervention with Sargassum fusiforme (SF) and Sargassum fusiforme fucoidan (SFF) through integration of 16S rRNA sequencing, transcriptomics, and non-targeted metabolomics. …”
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De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle by Teng Xu, Xudong Guo, Hui Wang, Xiaoyuan Du, Xiaoyu Gao, Dongjun Liu

“…In the present study, using Illumina/Solexa sequencing technology, we de novo assembled 130 million mRNA-Seq reads into a total of 49,115 contigs. …”
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Expression of the Gene Encoding the Brucella abortus BAB1-0278 Antigen in Probiotic Lactococcus lactis Bacteria by donya kazemi, abbas doosti, mostafa Shakhsi niaee

“…Materials and Methods: The gene construction design contains a signal peptide sequence in the nisin-based expression vector (pN3z8148-Usp45-BAB1-0278) and is synthesized by GENEray. …”
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