Effects of drought stress and Morchella inoculation on the physicochemical properties, enzymatic activities, and bacterial community of Poa pratensis L. rhizosphere soil by Wei Yin, Le Wang, Qian Han Shang, Yi Xin Li, Wei Sa, Quan Min Dong, Jian Liang

“…Methods High-throughput sequencing was employed to study rhizosphere soil bacterial communities in both Morchella-inoculated and uninoculated Poa pratensis rhizosphere soil subjected to moderate (50% soil moisture) and severe (30% soil moisture) drought stress, as well as under normal water conditions (70% soil moisture). …”
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Comparative Transcriptomic Analysis of Root Cadmium Responses in Two Chinese Rice Cultivars Yuzhenxiang and Xiangwanxian 12 by Shangdu Zhang, Zhenliang Luo, Xiang Wu, Bangzhi Shi, Huidan Jiang, Leliang Zhou, Lianyang Bai

“…In this study, the results of RNA sequencing (RNA-seq) showed that there were 341 and 161 differentially expressed genes in the roots of YZX and XWX after Cd exposure, respectively. …”
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Tumor microbiome: roles in tumor initiation, progression, and therapy by Shengxin Zhang, Jing Huang, Zedong Jiang, Huan Tong, Xuelei Ma, Yang Liu

“…With the application of 16S ribosomal ribonucleic acid (16S rRNA) sequencing, the intratumoral microbiome, also referred to as tumor-intrinsic or tumor-resident microbiome, has also been found to play a significant role in the tumor microenvironment (TME). …”
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The skin microbiota of preterm infants and impact of diaper change frequency. by Noelle E Younge, D Joshua Parris, Daniel Hatch, Angel Barnes, Debra H Brandon

“…The microbiome at each site was characterized by 16S rRNA gene sequencing. Associations between microbiome features, diaper change frequency, and other covariates were examined using mixed effect models and redundancy analysis.…”
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Metadag: a web tool to generate and analyse metabolic networks by Pere Palmer-Rodríguez, Ricardo Alberich, Mariana Reyes-Prieto, José A. Castro, Mercè Llabrés

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Comprehensive analysis of ceRNA Networks in UCEC: Prognostic and therapeutic implications. by Li Fan, Mengqiu Lan, Xiaohua Wei, Lili Wei, Liuhong Yang, Liuying Nong, Jiajia Wei, Jingjing Li, Wenjie Huang

“…In this study, we analyzed mRNA and miRNA sequencing data from UCEC tissues and adjacent non-cancerous tissues from the TCGA database. …”
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Responses of a soil-inhabiting collembolan (Entomobrya proxima Folsom) to organic fertilizer addition illustrated by functional traits and gut bacterial community by Xinyue Yang, Gang Li, Weiming Xiu

“…Stereomicroscopy and high-throughput amplicon sequencing were employed to elucidate the response of soil fauna to organic fertilizer through host functional traits and associated gut microbial communities.ResultsThe results indicated that the impact of organic fertilizer on the functional traits of collembolans was closely linked to the input concentration. …”
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Analysis of TGFBI Gene Mutations in Three Chinese Families with Corneal Dystrophy by Feng Zhao, Yuan Liu, Tao Guan

“…All 17 exons of the TGFBI gene, and the exon-intron junctions were examined by polymerase chain reaction (PCR) and direct DNA sequencing to identify and analyse gene mutations. In addition, all members of the three families were subjected to detailed clinical examinations. …”
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Mahuang Fuzi Xixin Decoction Ameliorates Allergic Rhinitis in Rats by Regulating the Gut Microbiota and Th17/Treg Balance by Xiao Liang, Chang-Shun Liu, Xiao-Han Wei, Ting Xia, Fei-Long Chen, Qing-Fa Tang, Meng-Yue Ren, Xiao-Mei Tan

“…The structure of the gut microbiota was analyzed by 16S rRNA gene sequencing and qPCR. Short-chain fatty acid (SCFA) content in rat stool and serum was determined by GC-MS. …”
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Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine by Guoxin Shi, Yuanyuan Jia, Lei Bai, Guoqiang Li, Xiaoli Liu, Meichen Liu, Xuecheng Dai, Xuefeng Tian, Hongbo Wang, Ting Ma

“…Six formulas were selected for the stimulation of indigenous microbes and to compare their performances on the oil emulsification and oil spreading in the Luliang oilfield containing Ca2+-rich brine. Illumina MiSeq sequencing of 16S rRNA genes was applied to investigate the structural response of microbial communities to various formulas. …”
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Comparison of SNP Variation and Distribution in Indigenous Ethiopian and Korean Cattle (Hanwoo) Populations by Zewdu Edea, Hailu Dadi, Sang Wook Kim, Tadelle Dessie, Kwan-Suk Kim

“…Although a large number of single nucleotide polymorphisms (SNPs) have been identified from the bovine genome-sequencing project, few of these have been validated at large in Bos indicus breeds. …”
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Identification of molecular characteristics in polycystic ovary syndrome using single-cell and transcriptome analysis by Xiaolin Zhu, Yanhua Han, Yuenan Feng, Yuanli Shan, Chang Liu, Kexin Wang, Xiaoke Li, Shidi Zhang, Yaguang Han

“…Gene expression profiles from GSE138518 and GSE155489, and single-cell RNA sequencing (scRNA-seq) data from PRJNA600740 were collected and subjected to bioinformatics analysis to identify the complex molecular mechanisms of PCOS. …”
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Differentiation of Lynch syndrome from other forms of non-polyposis colorectal cancer among Russian patients by A. S. Tsukanov, N. I. Pospehova, V. P. Shubin, I. Yu. Sachkov, S. N. Zhdankina, A. A. Ponomarenko, E. G. Rybakov, S. I. Achkasov, V. N. Kashnikov, S. A. Frolov, Yu. A. Shelygin

“…At its detection search of germline mutations by polymerase chain reaction methods, electrophoresis and direct sequencing was carried out.Results. Microsatellite instability was found out in 17 neoplastic samples (23%, 17 of 73). …”
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Temporal Study of Environmental DNA and Acoustic Data Reveals Coexistence of Sympatric Bat Species in a North American Ecosystem by Vrinda M. Suresh, Trevor Hébert, Kirsten Isabel Verster, Elizabeth A. Hadly

“…We used two different methods to assess niche partitioning among insectivorous bats at a site in the San Francisco Bay Area, California: (1) eDNA sequencing of bat feces that were collected weekly from a bat roost, and (2) nightly acoustic recordings of ultrasonic bat calls from recorders at multiple sites. …”
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Clinical utility of tumour mutational burden on efficacy of immune checkpoint inhibitors in malignant solid tumours: protocol for a systematic review and meta-analysis by Xiaoguang Yang, Yunming Li, Xuemei Xiang, Wang Guo, Pengfei Zhou

“…We plan to conduct a subgroup analysis on age, sex, area, number of patients (high/low TMB), cancer type, tumour size, stage, line of therapy, TMB sequencing method, type of immunotherapy and follow-up period.Ethics and dissemination Ethical approval and informed consent are not needed, as the study will be a literature review and will not involve direct contact with patients or alterations to patient care. …”
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What Drives Embryo Development? Chromosomal Normality or Mitochondria? by A. Bayram, I. Elkhatib, A. Arnanz, A. Linan, F. Ruiz, B. Lawrenz, H. M. Fatemi

“…Preimplantation genetic screening (PGS) and mitochondrial DNA (mtDNA) copy number were done using next generation sequencing (NGS). The only normal embryo among the all-biopsied embryos had the highest “Mitoscore” value and was the only arrested embryo in both cases. …”
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Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells by Beibei Chen, Bo Zhang, Huaxia Luo, Jiao Yuan, Geir Skogerbø, Runsheng Chen

“…We separated breast cancer cell nuclei from cytoplasm, and identified small RNA sequences using a high-throughput sequencing platform. …”
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TGF-β signaling orchestrates cancer-associated fibroblasts in the tumor microenvironment of human hepatocellular carcinoma: unveiling insights and clinical significance by Junwei Ge, Hongwei Jiang, Junjun Chen, Xuemin Chen, Yue Zhang, Liangrong Shi, Xiao Zheng, Jingting Jiang, Lujun Chen

“…In our recent study, drawing from the GEO database, we identified two fibroblast subtypes in HCC through single-cell RNA sequencing (scRNA-seq) and explore the expression and distribution of TGF-β and its receptors in the TME of HCC. …”
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Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma by Kawuli Jumai, Tangjuan Zhang, Bingzhang Qiao, Julaiti Ainiwaer, Haiping Zhang, Zhichao Hou, Idris Awut, Madinyat Niyaz, Liwei Zhang, Ilyar Sheyhidin

“…We further explored the cancer-promoting mechanism of target genes in ESCC by sequencing of the genes’ exons. Results. Scavenger receptor class A member 5 (SCARA5) was proved to be the downstream driving gene of THSD7A. …”
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The effect of sulfadiazine in manure on accumulation of sulfonamide resistance genes in freshly consumable plants by Leo van Overbeek, Björn Berendsen, Stefan Aanstoot, Alex Bossers, Els Nijhuis, Lina Russ, Beatriz Andreo Jimenez

“…Further, the impact of SDZ in manure on bacterial community and antibiotic resistance gene composition via amplicon sequencing and shotgun metagenomics was investigated in rhizosphere soils. …”
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