Interconnection algorithm of a wide range of pervasive devices for the Internet of things by Tao Chi, Ming Chen

“…Our approach is to perform it independently within the given task, where the given task can be achieved with its sequencing graph entities, including automate selection, binding, and scheduling. …”
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An evaluation of the severe acute respiratory infection surveillance system in Ireland by Louise Marron, Roisin Duffy, Joan O’Donnell, Lisa Domegan

“…Time to receipt of whole genome sequencing (WGS) results was 40 days (IQR 30–61). Themes identified in the qualitative evaluation were the complexity of SARI surveillance, dependence on key individuals and skilled staff. …”
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Hypermutability bypasses genetic constraints in SCV phenotypic switching in Pseudomonas aeruginosa biofilms by Romina A. Tobares, Román A. Martino, Claudia A. Colque, Gaston L. Castillo Moro, Alejandro J. Moyano, Andrea G. Albarracín Orio, Andrea M. Smania

“…Through experimental evolution and whole-genome sequencing, we show that both wild-type and mutator strains undergo parallel evolution by accumulating compensatory mutations in factors regulating intracellular c-di-GMP levels, particularly in the Wsp and Yfi systems. …”
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Seed banking impacts native Acacia ulicifolia seed microbiome composition and function by Dylan Russell, Vaheesan Rajabal, Matthew Alfonzetti, Marlien M. van der Merwe, Rachael V. Gallagher, Sasha G. Tetu

“…In this study we investigated the composition and functional potential of seed-associated bacterial epiphytes associated with stored and freshly collected seeds of a native plant, Acacia ulicifolia, using both 16S rRNA gene sequencing and culture-based approaches. Results Seeds obtained from seed banking facilities were found to host significantly less diverse bacterial populations, with substantial reductions in both low-abundance taxa and in community members commonly identified in freshly collected A. ulicifolia seeds. …”
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Isolation, identification, and characterisation of the malachite green detoxifying bacterial strain Bacillus pacificus ROC1 and the azoreductase AzrC by Shanza Bibi, Callum W. Breeze, Vusqa Jadoon, Anum Fareed, Alina Syed, Rebecca L. Frkic, Habiba Zaffar, Muhammad Ali, Iftikhar Zeb, Colin J. Jackson, Tatheer Alam Naqvi

“…We demonstrate immobilized B. pacificus ROC1 can effectively detoxify MG, which highlights a potential method for its biodegradation. Genomic sequencing identified three candidate azo-reductases within B. pacificus ROC1 that could be responsible for the MG-degrading activity. …”
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Association of β-Catenin, APC, SMAD3/4, Tp53, and Cyclin D1 Genes in Colorectal Cancer: A Systematic Review and Meta-Analysis by Hongfeng Yan, Fuquan Jiang, Jianwu Yang

“…The authors searched PubMed, EMBASE, and Science Direct for relevant reports published between 2000 and 2020 and analyzed them to determine any relationship between the (immunohistochemically/sequencing-detected) gene expression and variants of the selected genes and the survival of CRC patients. …”
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A pathogenic COL7A1 variant highlights semi-dominant inheritance in dystrophic epidermolysis bullosa by Saira Sattar, Thashi Bharadwaj, Umm-e- Kalsoom, Anushree Acharya, Saadullah Khan, Suzanne M. Leal, Isabelle Schrauwen

“…We investigated a Pakistani family with dystrophic epidermolysis bullosa via exome sequencing and identified a pathogenic nonsense variant in COL7A1 NM_000094 c.1573 C > T:p.…”
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Expanded non-invasive prenatal testing offers better detection of fetal copy number variations but not chromosomal aneuploidies. by Shaozhe Yang, Yuan Zhuang, Junfeng Li, Xiuhong Fu

“…<h4>Conclusion</h4>By enhancing sequencing data, NIPT-plus can effectively screen for CNVs as well as chromosomal aneuploidies. …”
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Severe Wound Infection with Photobacterium damselae ssp. damselae and Vibrio harveyi, following a Laceration Injury in Marine Environment: A Case Report and Review of the Literatur... by Jörg Hundenborn, Steffi Thurig, Mechthild Kommerell, Heike Haag, Oliver Nolte

“…Identification was achieved by use of Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry (MALDI-TOF) and confirmed by 16S rDNA sequencing. Data retrieval from bibliography was complicated since P. damselae has been renamed often with a number of synonyms present in the literature: Photobacterium damsela, Vibrio damselae, Vibrio damsela, Pasteurella damselae, and Listonella damsela. …”
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A Near Complete Genome Assembly of the Oshima Cherry Cerasus speciosa by Kazumichi Fujiwara, Atsushi Toyoda, Bhim B. Biswa, Takushi Kishida, Momi Tsuruta, Yasukazu Nakamura, Noriko Kimura, Shoko Kawamoto, Yutaka Sato, Toshio Katsuki, Sakura 100 Genome Consortium, Tsuyoshi Koide

“…Using Illumina short-read, PacBio long-read, and Hi-C sequencing, we constructed a 269.3 Mbp genome assembly with a contig N50 of 32.0 Mbp. …”
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G3DC: A Gene-Graph-Guided Selective Deep Clustering Method for Single Cell RNA-seq Data by Shuqing He, Jicong Fan, Tianwei Yu

“…Single-cell RNA sequencing (scRNA-seq) technology measures the expression of thousands of genes at the cellular level. …”
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Pea (Pisum sativum L.): the uneasy fate of the first genetical object by O. E. Kosterin

“…Meanwhile, we await the completion of pea nuclear genome sequencing in 2016. For working out new molecular markers in pea, the synteny of its genome to the sequenced genome of Medicago truncatula is extensively used. …”
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Case report: Infantile pulmonary alveolar proteinosis associated with cytosolic isoleucyl-tRNA synthetase deficiency by Jie Wu, Yimu Fan, Feng Huo, Jie Deng, Quan Wang, Yuelin Shen, Yuelin Shen

“…Additionally, whole-exome sequencing identified compound heterozygous variants in the IARS1 gene (c.2428C&gt;T/c.128T&gt;C), both of which are novel observations.…”
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Phosphoryl guanidine oligonucleotides as primers for RNA-dependent DNA synthesis using murine leukemia virus reverse transcriptase by E. S. Dyudeeva, I. A. Pyshnaya

“…It was shown that the proportion (%) of abortive elongation products of a PGO primer depends on buffer ionic strength, nucleotide sequence of the primer, and the presence and location of phosphoryl guanidine groups in the primer. …”
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EBV-Negative Monomorphic B-Cell Posttransplant Lymphoproliferative Disorder with Marked Morphologic Pleomorphism and Pathogenic Mutations in ASXL1, BCOR, CDKN2A, NF1, and TP53 by Agata M. Bogusz

“…Fluorescence in situ hybridization studies (FISH) were negative for cMYC, BCL2, and BCL6 rearrangements but showed deletion of TP53 and monosomy of chromosome 17. Next-generation sequencing studies (NGS) revealed numerous genetic alterations including 6 pathogenic mutations in ASXL1, BCOR, CDKN2A, NF1, and TP53(x2) genes and 30 variants of unknown significance (VOUS) in ABL1, ASXL1, ATM, BCOR, BCORL1, BRNIP3, CDH2, CDKN2A, DNMT3A, ETV6, EZH2, FBXW7, KIT, NF1, RUNX1, SETPB1, SF1, SMC1A, STAG2, TET2, TP53, and U2AF2.…”
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A Recurrent Nonsense Mutation in NECTIN4 Underlying Ectodermal Dysplasia-Syndactyly Syndrome with a Novel Phenotype in a Consanguineous Kashmiri Family by Ghazanfar Ali, Sadia Sadia, Syeda Ain-ul- Batool, Zahid Azeem, Naheed Bashir Awan, Syed Akif Raza Kazmi, Zia- Ur- Rehman, Zeeshan Anjum, Fazal- Ur- Rehman, Abdul Wali, Kafaitullah Khan, Nasib Zaman, Muhammad Ayub, Muhammad Sajid, Noor Hassan

“…Here, we report a consanguineous family of Kashmiri origin presenting features of EDSS1. Using whole exome sequencing, we found a recurrent nonsense mutation (NM_030916: c.181C > T, p.…”
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Eye tracking based clustering using the Korean version of the reading the mind in the eyes test by Se Jun Koo, Eun Jung Cha, Jee Eun Min, Eunchong Seo, Eun Lee, Suk Kyoon An

“…The long word fixation time cluster had significantly more total errors and fewer categories completed in the Wisconsin Card Sorting Test, lower backward Digit Span score, and lower sequencing scores in the Theory of Mind Picture Stories Task than in the other clusters. …”
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Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea by Marianna Marangi, Sonia Boughattas

“…A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to Blastocystis sp. using molecular tools. …”
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Dynamics of Salmonella Dublin infection and antimicrobial resistance in a dairy herd endemic to salmonellosis. by Victor Santos do Amarante, Joelma Kellen de Castro Pereira, Matheus Ferreira Serafini, Carolina Pantuzza Ramos, Isabela Pádua Zanon, Thayanne Gabryelle Viana de Souza, Tiago Facury Moreira, Antônio Ultimo de Carvalho, Rodrigo Melo Meneses, Flavia Figueira Aburjaile, Vasco Azevedo, Elias Jorge Facury Filho, Rodrigo Otávio Silveira Silva

“…Seven multidrug-resistant isolates were selected for genomic sequencing. Among the resistance determinants identified, a mutation in the gyrA gene, present in all sequenced isolates, was notable. …”
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A Rare Co-Occurrence of Triple Mutations in JAK2, CALR, and MPL in the Same Patient with Myelofibrosis by Sherine J. Thomas, D. P. Dash

“…The above case in addition to recent case reports and case series supports the use of broader NGS sequencing panels for diagnosis and prognostication of MPN. …”
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