Dispensable genome and segmental duplications drive the genome plasticity in Fusarium solani by Abbeah Navasca, Jatinder Singh, Viviana Rivera-Varas, Upinder Gill, Gary Secor, Thomas Baldwin

“…We assembled the chromosome-level genome of the F. solani sugarbeet isolate strain SB1 using Oxford Nanopore and Hi-C sequencing. The average size of F. solani genomes is 54 Mb, whereas SB1 has a larger genome of 59.38 Mb, organized into 15 chromosomes. …”
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Effects of Salmonella Typhimurium infection on intestinal flora and intestinal tissue arachidonic acid metabolism in Wenchang chickens by Shenghong Chen, Yaochen Xie, Dingqian Guo, Tiansen Li, Zhen Tan, Xuhua Ran, Xiaobo Wen

“…Typhimurium using 16s rDNA sequencing and targeted metabolomics. The results showed that the levels of ARA metabolites were increased in the cecum tissue of Wenchang chickens after infection with S. …”
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Generation of barcoded plasmid libraries for massively parallel analysis of chromatin position effects by M. O. Lebedev, L. A. Yarinich, A. V. Ivankin, A. V. Pindyurin

“…First, we highlight the critical parameters that should be considered in the generation of barcoded plasmid libraries and introduce a simple method to assess the diversity of random sequences (barcodes) of synthetic oligonucleotides using PCR amplification followed by Sanger sequencing. …”
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Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction by Reza Yesica, Andreas Bandang Hardian, Yudit Oktanella, Shelly Kusumarini, Gabriela Jeanne Giacynta Prasetyo

“…One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. …”
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Prediction of the Sex-Associated Genomic Region in Tunas (Thunnus Fishes) by Yoji Nakamura, Kentaro Higuchi, Kazunori Kumon, Motoshige Yasuike, Toshinori Takashi, Koichiro Gen, Atushi Fujiwara

“…In the present study, we sequenced a male genome of Pacific bluefin tuna by long-read and linked-read sequencing technologies and explored male-specific loci through a comparison with the female genome. …”
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Comprehensive Enteroviral Serology Links Infection and Anti‐Melanoma Differentiation‐Associated Protein 5 Dermatomyositis by Sahana Jayaraman, Eleni Tiniakou, William R. Morgenlander, Miso Na, Lisa Christopher‐Stine, H. Benjamin Larman

“…Methods We employed Phage ImmunoPrecipitation Sequencing to profile serum antibodies against the human proteome, the human virome, and a comprehensive enterovirus library. …”
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Differential Diagnosis of MutYH-Associated Polyposis from Sporadic Colon Polyps by A. S. Tsukanov, V. P. Shubin, A. M. Kuzminov, M. Kh. Toboeva, T. A. Savelyeva, V. N. Kashnikov, Yu. A. Shelygin

“…A search for mutations in the MutYH gene was carried out using the PCR, electrophoresis and direct sequencing methods among the following groups of people: 18 patients under 45 years old with more than 100 polyps diagnosed in the large intestine; 86 patients over 45 years old with 4–100 polyps; 150 people of the control group.Results. …”
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Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation in Scylla paramamosain by Huan Wang, Hongling Wei, Lei Tang, Junkai Lu, Changkao Mu, Chunlin Wang

“…This study performed a correlation analysis of RNA sequencing transcriptomics and iTRAQ proteomics in order to investigate the adaptation mechanisms to sudden salinity drop from 23‰ to 3‰. …”
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Prevalence of Drug Resistance and Associated Mutations in a Population of HIV-1+ Puerto Ricans: 2006–2010 by Lycely del C. Sepúlveda-Torres, Alexandra De La Rosa, Luz Cumba, Nawal Boukli, Eddy Ríos-Olivares, Luis A. Cubano

“…Samples from 2,500 patients from 2006–2010 were analyzed using the TruGene HIV-1 genotyping kit and the OpenGene DNA sequencing system. Results show that 58.8% of males and 65.3% of females had HIV-1 with resistance to at least one medication. …”
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Studies on the reduction of organic load from palm oil mill effluent (POME) by bacterial strains by Jeremiah David Bala, Japareng Lalung, Norli Ismail

“…The main aim of the present study was to investigate the ability of bacterial strains either pure (individually) or mixed (combinations), to degrade and metabolize organic load from palm oil mill effluent. Results Sequencing of the 16S rRNA of the isolates suggests that they were identified as Micrococcus luteus 101 PB, Stenotrophomonas maltophilia 102 PB, Bacillus cereus 103 PB, Providencia vermicola 104 PB, Klebsiella pneumoniae 105 PB and Bacillus subtilis 106 PB. …”
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The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al-Najaf, Iraq by Aoula Al-Zebeeby, Ali Hadi Abbas, Haider Abas Alsaegh, Furkan Sabbar Alaraji

“…The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. …”
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Some limitations in the use of the mitochondrial DNA cytb gene as a molecular marker for phylogenetic and population genetic studies by the example of the Apodemus genus by A. G. Lapinski, M. V. Pavlenko, L. L. Solovenchuk, V. V. Gorbachev

“…Emphasis is placed on the necessity of close control over sequencing data.…”
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The Alpaca Melanocortin 1 Receptor: Gene Mutations, Transcripts, and Relative Levels of Expression in Ventral Skin Biopsies by Bathrachalam Chandramohan, Carlo Renieri, Vincenzo La Manna, Antonietta La Terza

“…Full length cDNA amplification revealed the presence of two transcripts, named as F1 and F2, differing only in the length of their 5′-terminal untranslated region (UTR) sequences and presenting a color specific expression. …”
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Efficacy of CLT4A variants as immunoregulatory molecules among Vitiligo patients in Saudi Arabia by Amal F. Alshammary, Ghada BinSaif, Imran Ali Khan

“…Extracted genomic DNA was utilized to amplify rs231775 and rs3087243 SNPs in the CTLA4 gene, which were subsequently digested and verified using Sanger sequencing. The polymerase chain reaction and restriction fragment length polymorphism analysis laboratory data were stored in Excel and used for further statistical analysis. …”
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Challenges in Medicine: The Odyssey of a Patient with Isolated IgG4-Related Eosinophilic Angiocentric Fibrosis Presenting as a Locally Destructive Sinonasal Mass by Snigdha Nutalapati, Richard O’Neal, William O’Connor, Brett T. Comer, Gerhard C. Hildebrandt

“…Our case interestingly also had T-cell clonality and isolated isocitrate dehydrogenase 2 enzyme mutation on next-generation sequencing, suggesting a possible role of novel molecular-targeted therapies in this rare disease. …”
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A Comparative Study on Growth Performance, Body Composition, and Liver Tissue Metabolism Rearing on Soybean Lecithin-Enriched Artemia Nauplii and Microdiet in Rock Bream (Oplegnath... by Pian Zhang, Peng Tan, Lei Zhang, Wenliang Zhu, Ruiyi Chen, Ligai Wang, Dongdong Xu

“…Larvae fed a MD showed decreased lipolysis-related lipase activity as well as decreased amino acid catabolism-related alanine aminotransferase and aspartate aminotransferase enzyme activities in liver tissue. RNA sequencing revealed that feeding with the MD primarily increased the expression of lipogenesis-related genes and protein translation-related gene expression in the liver tissue. …”
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Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) by Louise Bach Kmetiuk, Louise Bach Kmetiuk, Ana Silvia Miranda Passerino, Marcelo Bonat, Vívien Midori Morikawa, Alice Pereira Berbigier, André Luiz Rodrigues Roque, Fabiano Borges Figueiredo, Alexander Welker Biondo

“…In overall, 4/17 (23.5%) spider monkeys were positive to Trypanosoma spp. by PCR. Sequencing results for 18S rDNA revealed Trypanosoma minasense infection. …”
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A Survey of Wild Indigenous <i>Cryptostylis ovata</i> Orchid Populations in Western Australia Reveals Spillover of Exotic Viruses by Stephen Wylie, Hua Li, Shu Hui Koh

“…Eighty-three <i>C. ovata</i> samples from 16 populations were collected, and sequencing was used to identify RNA viruses from them. …”
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Preliminary screening of new biomarkers for sepsis using bioinformatics and experimental validation. by Hao Wang, Wei Xiong, Wu Zhong, Yingchun Hu

“…The core genes were localized using single-cell sequencing. qPCR was used to validate the core genes.…”
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Molecular Detection and Genotyping of Chlamydia psittaci in Captive Psittacines from Costa Rica by Jessica Sheleby-Elías, Ántony Solórzano-Morales, Juan José Romero-Zuñiga, Gaby Dolz

“…Three birds were adults; all positive birds showed no symptoms of illness and lived in homes with other birds, two in San José and two in Heredia. Sequencing was used to confirm the PCR positive results, showing that two samples of C. psittaci belonged to genotype A, representing the first report of the presence of this genotype in Costa Rica. …”
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