Bifidobacterium animalis subsp. lactis TG11 ameliorates loperamide-induced constipation in mice by modulating gut microbiota by Weiwei Ma, Yiyang Zhao, Yuyang Liu, Yanyan Wang, Shuang Yu, Lili Huang

“…TG11 markedly upregulated mRNA expression of SCF and c-kit in colonic tissue. Metagenomic sequencing revealed that TG11 modulated gut microbiota composition, increasing the abundance of beneficial bacteria, particularly Muribaculum_sp. and uncultured_Duncaniella.DiscussionBifidobacterium animalis subsp. lactis TG11 demonstrates efficacy in ameliorating constipation, potentially through modulation of the gut microbiota composition.…”
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The Shared and Specific Genes and a Comparative Genomics Analysis within Three Hanseniaspora Strains by Kai Chen, Zhonghuan Tian, Fatang Jiang, Yunjiang Cheng, Chao-an Long

“…This study was based on the previous genome sequencing of K. apiculata strain 34-9. After homologous comparison, scaffold 27 was defined as the mitochondrial (mt) sequence of K. apiculata 34-9. …”
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Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps by Jiachao Xiong, Zhixiao Liu, Minliang Wu, Mengyan Sun, Yu Xia, Yuchong Wang

“…We analyzed the differential microRNA (miRNA) expression in ADSC-Exo using next-generation sequencing (NGS) technology to explore their potential mechanisms in promoting vascularization. …”
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The southern house mosquito Culex quinquefasciatus in Abu Dhabi, UAE, is developing resistance to deltamethrin insecticide by Amgd sayed Ali, Asim Iqbal, Tamilarasan Kamalanathan, Antony Raj Gnanaprakasam, Sabu Shajahan, Mohammad Hamad Alsadeq, Mohamed Mahmood Al Marzooqi, Mohamad Hamdan, Mohammad Ali Al-Deeb

“…We screened Cx. quinquefasciatus adults (N = 174) for the presence of the mutation using allele-specific PCR (AS-PCR) and DNA sequencing. We detected the mutation and found the kdr allele in all the sampled locations. …”
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High-throughput method characterizes hundreds of previously unknown antibiotic resistance mutations by Matthew J. Jago, Jake K. Soley, Stepan Denisov, Calum J. Walsh, Danna R. Gifford, Benjamin P. Howden, Mato Lagator

“…We present a high-throughput technique – Quantitative Mutational Scan sequencing (QMS-seq) – that enables quantitative comparison of which genes are under antibiotic selection and captures how genetic background influences resistance evolution. …”
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Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p by Biao Wang, Nianjie Zhang, Lin Dai, Yuanwei Zhang, Shuo Yin, Xuefeng Yang

“…Our previous research demonstrated that NP could promote the proliferation and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells; however, the specific mechanism remains unclear. miRNA sequencing revealed that NP upregulated the expression levels of microRNA(miR)-151a-3p in CRC. …”
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The occurrence of wheat crown rot correlates with the microbial community and function in rhizosphere soil by Yajiao Wang, Jian Feng, Jianhai Gao, Sen Han, Qiusheng Li, Lingxiao Kong, Yuxing Wu

“…In this study, rhizosphere soils with varying WCR severities (light, moderate, severe) were analyzed for chemical properties, microbial community composition and functions using high-throughput sequencing. The results revealed that WCR decreased rhizosphere soil pH, the content of available nitrogen and phosphorus, and the abundance of beneficial taxa such as Bacillus and Streptomyces. …”
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Short-term evolutionary implications of an introgressed size-determining supergene in a vulnerable population by Pierre Lesturgie, John S. S. Denton, Lei Yang, Shannon Corrigan, Jeff Kneebone, Romuald Laso-Jadart, Arve Lynghammar, Olivier Fedrigo, Stefano Mona, Gavin J. P. Naylor

“…We conducted whole genome sequencing of samples collected across its range. Genetic diversity was similar at all sampled sites, but we discovered a ~ 31 megabase bi-allelic supergene associated with the size polymorphism, with the larger size allele having introgressed in the last ~160,000 years B.P. …”
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From threats to solutions: A literature review of climate adaptation in anadromous salmon and trout by Lisa G. Crozier, Jared E. Siegel

“…By capitalizing on new tools for sequencing, genomic analysis, and automated field data collection, we can establish baselines for tracking evolutionary responses to climate change. …”
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Decoding the molecular, cellular, and functional heterogeneity of zebrafish intracardiac nervous system by Andrea Pedroni, Elanur Yilmaz, Lisa Del Vecchio, Prabesh Bhattarai, Inés Talaya Vidal, Yu-Wen E. Dai, Konstantinos Koutsogiannis, Caghan Kizil, Konstantinos Ampatzis

“…Here, we present a comprehensive taxonomy of the IcNS, utilizing single-cell RNA sequencing, anatomical studies, and electrophysiological techniques. …”
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PRKD2 as a novel target for targeting the diabetes–osteoporosis nexus by Rongjin Chen, Chenhui Yang, Hefang Xiao, Ao Yang, Changshun Chen, Fei Yang, Bo Peng, Bin Geng, Yayi Xia

“…By employing the Mfuzz time-series gene clustering method combined with transcriptome sequencing of patient serum, we systematically delineated gene expression patterns during the transition from a healthy state through DM to DMOP. …”
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Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq by Sura S. Aghwan, Enas S. Hussein, Salam A Esmaeel

“…The infection rate of Cytauxzoon spp. among cats in Mosul city was 76% (38/50). Using single sequence analysis, a single Cytauxzoon felis sequence was discovered in Nineveh Governorate for the first time. …”
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« L’épopée » des clandestins by Zied Hadfi

“…Here the interviewee is speaking for the entire community of illegal immigrants, describing their suffering, their fear, but also their hopes and their success. Through sequencing of various pronominal forms "they", "we", « them" ... …”
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Placental Infection with Different SARS-CoV-2 Variants Leading to Stillbirth: Report of Two Cases by Miriam A. Snow, Medini K. Annavajhala, Sofia Z. Moscovitz, Anne-Catrin Uhlemann, Larisa Debelenko

“…Placental tissues were positive for SARS-CoV-2 by in situ hybridization (ISH) and immunohistochemistry (IHC). Sequencing revealed the delta variant in Case 1 and omicron XBB.1.515 in Case 2. …”
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Comprehensive Analysis Identifies <i>THEMIS2</i> as a Potential Prognostic and Immunological Biomarker in Glioblastoma by Jianan Chen, Qiong Wu, Anders E. Berglund, Robert J. Macaulay, Arnold B. Etame

“…Additionally, single-cell RNA sequencing data demonstrated that macrophages with a high <i>THEMIS2</i> expression were associated with increased phagocytosis, immune suppression, and enhanced tumor growth. …”
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Swine zoonotic viruses: transmission and novel diagnostic technology by Letian Zhang, Zhiwen Jiang, Ying Qin, Yuhai Bi, Shuo Su

“…Additionally, we emphasized novel detection techniques including rapid diagnostic tests, biosensor-based detection technology, high-throughput sequencing, and systematic viral epitope scanning. These techniques are instrumental in enabling cost-effective and convenient rapid detection procedures for broader implementation across diverse regions for effective surveillance of viral epidemics. …”
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Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) by Katarzyna Krawczyk, Joanna Szablińska-Piernik, Łukasz Paukszto, Mateusz Maździarz, Paweł Sulima, Jerzy Andrzej Przyborowski, Monika Szczecińska, Jakub Sawicki

“…In this study, the chromosome-scale telomere-to-telomere genome of R. sorocarpa was assembled exclusively by Oxford Nanopore long-read sequencing and Pore-C technology. A high-quality chromosomal-scale assembly was obtained with a final genome size of 376.690 Mbp, contig N50 of 49.132 Mbp and 97.02% of the assembled contigs associated with the eight chromosomes. …”
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Multi-omics dataset of individual variations in growth performance of large yellow croaker by Hao Huang, Zhenheng Cheng, Yabing Wang, Guangde Qiao, Xiaoshan Wang, Yanfeng Yue, Quanxin Gao, Shiming Peng

“…We constructed association models for “gut microbiota–differentially expressed genes”, “differentially expressed genes–metabolites,” and “gut microbiota–metabolites.” Sequencing data and LC–MS/MS raw data have been deposited in NCBI and MetaboLights databases for public access. …”
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Generation of IgM+ B cell-deficient Atlantic salmon (Salmo salar) by CRISPR/Cas9-mediated IgM knockout by Mari Raudstein, Ma. Michelle D. Peñaranda, Erik Kjærner-Semb, Søren Grove, H. Craig Morton, Rolf Brudvik Edvardsen

“…In this study, we used CRISPR/Cas9 technology to knock out the two IgM genes in Atlantic salmon. High-throughput sequencing revealed an average mutagenesis efficiency of 97% across both loci, with a predominance of frameshift mutations (78%). …”
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MAIT cells protect against sterile lung injury by Xiawei Zhang, Shuailin Li, Wojciech Lason, Maria Greco, Paul Klenerman, Timothy S.C. Hinks

“…Using single-cell RNA sequencing (scRNA-seq), spectral analysis, and adoptive transfer in a bleomycin-induced sterile lung injury, we found that bleomycin activates murine pulmonary MAIT cells and is associated with a protective role against bleomycin-induced lung injury. …”
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