Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave by Pengfei Li, Jinzhu Meng, Wenzhong Liu, George W. Smith, Jianbo Yao, Lihua Lyu

“…For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. …”
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Morphological and Molecular Characteristics of Endophytic Fungi in Sugarcane as Antagonists of the Pathogen Fusarium sacchari by Asniah Asniah, Muhammad Taufik

“…Morphological identification was based on colony and microscopic characteristics, while molecular identification was conducted using PCR and sequencing, with similarities based on GenBank. Inhibition tests were conducted through dual culture and volatile compound testing, as well as enzyme production. …”
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Quantitative evaluation of the molecular marker using droplet digital PCR by Wonseok Shin, Haneul Kim, Dong-Yep Oh, Dong Hee Kim, Kyudong Han

“…In a previous study, we identified the Hanwoo-specific SV that was generated by a TE–association deletion event using traditional PCR method and Sanger sequencing validation. It could be used as a molecular marker to distinguish different cattle breeds (i.e., Hanwoo vs. …”
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Apolipoprotein C-II Deposition Amyloidosis: A Potential Misdiagnosis as Light Chain Amyloidosis by Sadichhya Lohani, Emily Schuiteman, Lohit Garg, Dhiraj Yadav, Sami Zarouk

“…Further evaluation of biopsy specimens with laser microdissection and mass spectrometry and DNA sequencing of exons should be done routinely in patients with amyloidoses for definitive diagnosis. …”
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Study on the Effect of Radish Sprouts on Short-Chain Fatty Acids and Gut Microbial Diversity in Healthy Individuals by Ru Li, Xuehong Chen, Cong Shi, Yi Zhu

“…The influence of radish sprouts on the gut microbiota was evaluated by monitoring short-chain fatty acid (SCFA) content and proportion in the fermentation broth, and microbial diversity was assessed using 16S rDNA amplicon sequencing. The results indicated that the gut microbiota produced a substantial amount of SCFA within 48 h of fermentation, with a right-skewed distribution across all groups. …”
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Environmental Metagenomic Analysis of "ESKAPE" Pathogens in the Pediatric Intensive Care Unit of General Hospital Yogyakarta Indonesia by Ludhang Pradipta Rizki, Indah Kartika Murni, Abu Tholib Aman, Titik Nuryastuti

“…We determine taxonomic profiles and also detect resistome, and virulome distribution of ESKAPE pathogens on various environmental surfaces through shotgun metagenomic sequencing. The microbiomes of the floor, sink, and mechanical ventilator exhibit a diverse composition of microbial communities, featuring significant species richness based on Shannon and Simpson’s index. …”
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Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese by Fengju Zhang, Tingzhun Zhu, Zhongjun Zhou, Yudong Wu, Yang Li

“…Genotyping was performed by direct sequencing after polymerase chain reaction(PCR) amplification and allele frequencies were tested for Hardy-Weinberg equilibrium. …”
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Effects of highland barley β-glucan on gut microbiota composition and metabolism in vitro fermentation by Yinchen Ge, Jiaci Liu, Huacheng Tang, Yanqing Zang, Yang Cao

“…Therefore, the effects of HBG on the gut microbiota during fermentation were analyzed by 16 s rRNA sequencing and untargeted metabolomics. The results showed that HBG could enrich microbial diversity, increase the abundance of beneficial bacteria, and inhibit the biology of pathogenic bacteria. …”
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Recent Advances in Antimicrobial Resistance: Insights from <i>Escherichia coli</i> as a Model Organism by Zhaoyang Zhang, Minliang Wei, Bin Jia, Yingjin Yuan

“…This review explores recent advances for investigating AMR in <i>E. coli</i>, including machine learning for resistance pattern analysis, laboratory evolution to generate resistant mutants, mutant library construction, and genome sequencing for in-depth characterization. Key resistance mechanisms are discussed, including drug inactivation, target modification, altered transport, and metabolic adaptation. …”
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A paradoxical population structure of var DBLα types in Africa. by Mun Hua Tan, Kathryn E Tiedje, Qian Feng, Qi Zhan, Mercedes Pascual, Heejung Shim, Yao-Ban Chan, Karen P Day

“…By applying a novel ups classification algorithm (cUps) to publicly-available DBLα sequence datasets, we categorised DBLα according to association with the three ups groups, thereby avoiding the need to sequence complete genes. …”
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Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation by Tarun Kumar Kumawat, Anima Sharma, Seema Bhadauria

“…This chicken feather degrading fungal strain previously isolated and identified by morphological and 18 s rDNA sequencing in laboratory, was used in the present study. …”
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Association of ANKRD55 Gene Polymorphism with HT: A Protective Factor for Disease Susceptibility by Yu-die Fang, Jing Zhao, Xin-juan Zhuang, Jian-bin Xu, Tian-tian Cai, Xiao-rong Yang, Kai-da Mu, Jin-an Zhang

“…Five loci (rs321776, rs191205, rs7731626, rs415407, and rs159572) of the ANKRD55 gene were genotyped using Multiplex PCR combined with high-throughput sequencing. Results. The results showed that the allele frequencies of rs7731626 and rs159572 loci in HT patients were lower than those in normal controls (P=0.048 and P=0.03, respectively). …”
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Leishmania major Infection in Synanthropic Rodents: Evidence for the Urbanization of Zoonotic Cutaneous Leishmaniasis (ZCL) in Southern Iran by Saeed Shahabi, Kourosh Azizi, Qasem Asgari, Bahador Sarkari

“…Leishmania DNA was detected in 18 (43.9%) rodents, including 11 R. rattus, 6 M. musculus, and one M. persicus, based on DNA sequencing of the ITS2 gene and PCR of the kDNA. Phylogenetic reconstruction revealed that the parasite infecting the rodents was L. major. …”
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Interleukin-16 Polymorphism Is Associated with an Increased Risk of Ischemic Stroke by Xiao-li Liu, Jian-zong Du, Yu-miao Zhou, Qin-fen Shu, Ya-guo Li

“…A total of 198 patients with ischemic stroke and 236 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. We found that the rs11556218TG genotype and G allele of IL-16 were associated with significantly increased risks of ischemic stroke (TG versus TT, adjusted OR = 1.88; 95% CI, 1.15–3.07; G versus T, adjusted OR = 1.54; 95% CI, 1.05–2.27, resp.). …”
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Molecular detection of some virulence gene in Proteus mirabilis isolated from chicken and human by Aamer Y. Alchalaby, Balsam Y. Rasheed, Ammar M. Al-AAlim

“…The standard bacteriological method was used for bacterial identification, and PCR confirmed the Proteus spp isolates. Further sequencing and phylogenetic analysis of zap A were conducted. …”
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Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age by T. Ząbek, E. Semik-Gurgul, T. Szmatoła, A. Gurgul, A. Fornal, M. Bugno-Poniewierska

“…We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. …”
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Molecular Determination of Toxocara spp. Eggs Isolated from Public Parks and Playgrounds in Zahedan, Southeast Iran by Siavash Liravizadeh, Samaneh Abdolahi Khabisi, Alireza Salimi Khorashad, Hadi Mirahmadi

“…Subsequently, all the sequenced data were blasted and compared with other sequences available in the GenBank. …”
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Label-Free Surface-Enhanced Raman Scattering for Genomic DNA Cytosine Methylation Reading by Kazi Morshed Alom, Anastasiia Tukova, Nana Lyu, Alison Rodger, Yuling Wang

“…Multiple approaches have been developed that employ extensive steps, such as bisulfite treatments, polymerase chain reactions (PCR), restriction digestion, sequencing, mass analysis, etc., to identify DNA methylation. …”
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The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran by Ali Taghipour, Majid Pirestani, Ramin Hamidi Farahani, Mohammad Barati

“…Moreover, the isolated sequences in the present study belonged to C. parvum subtype IIa and Enterocytozoon bieneusi genotypes D and Peru 8. …”
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Isolation and identification of fungi found in contaminated fermented milk and antifungal activity of vanillin by Xu Pei, Mekonen Tekliye, Mingsheng Dong

“…Fungal isolates obtained were characterized by DNA sequencing of the internal transcribed spacer (ITS) region. …”
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