Functional genomic imaging (FGI), a virtual tool for visualization of functional gene expression modules in heterogeneous tumor samples by Xinlei Chen, Youbing Guo, Xiaorong Gu, Danyi Wen

“…Abstract Advances in sequencing technologies are reshaping clinical diagnostics, prompting the development of new software tools to decipher big data. …”
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A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae by Jun Wu, Guomeng Li, Zhimou Lin, Yangzhi Zhang, Wenyuan Yu, Rong Hu, Shuai Zhan, Yazhou Chen

“…Notably, 96.19% of the assembled sequences were anchored to eight chromosomes. The genome covered 99.24% of BUSCO genes and 95.16% of CEGMA genes, indicating a high level of completeness. …”
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TLR-8, TNF-α, and ESR-1α Gene Polymorphism Susceptibility in Onset of Arthritis by Maryam Mukhtar, Nadeem Sheikh, Andleeb Batool, Tayyaba Saleem, Muhammad Babar Khawar, Mavra Irfan, Saira Kainat Suqaina

“…PCR-RFLP and direct sequencing methods were used for determining genetic variations. …”
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The Diversity of Airborne Bacteria Over the Tibet Plateau Decreased by Taklimakan Dust by Fanli Xue, Zhongwei Huang, Xuefei Huo, Qingqing Dong, Zhengpeng Li, Qianqing Gu

“…In the summer of 2019, we simultaneously collected air samples from the Tibetan Plateau (Ali) and the Taklimakan Desert (Minfeng and Tazhong) and analyzed them using high‐throughput DNA sequencing techniques. We found that the diversity of airborne bacteria decreased by 42.2% during dust events. …”
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Cultivable Fungi from Amazon River Dolphins Engaged in Wildlife Ecotourism in the Anavilhanas National Park, Brazil by Marla Jalene Alves, Fernanda Rodrigues Fonseca, Layssa do Carmo Barroso, Érica Simplício de Souza, Marcelo Derzi Vidal, Ani Beatriz Jackisch-Matsuura, João Vicente Braga de Souza, Salvatore Siciliano

“…Fungi were isolated using culture media and identified through micromorphology assays and ITS region sequencing. Oral swab samples resulted in culture of Trichosporon montevideense and Exophiala dermatitidis. …”
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Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells by Khaya Pearlman Shabangu, Manimagalay Chetty, Babatunde Femi Bakare

“…Taxonomic analysis using 16S/18S rDNA-targeted DGGE and high-throughput sequencing identified Proteobacteria as dominant in biorefinery biomass, followed by Firmicutes and Bacteriodota. …”
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Ophthalmomyiasis Case Caused by Two Blow Fly (Diptera: Calliphoridae) Species in North America by Taylor B. Parker, Kelly A. Meiklejohn, Gregory A. Dahlem, Ralph C. Eagle, Marius J. Heersink

“…A representative subsample of five larvae was used for taxonomic identification via two approaches (a) DNA analysis, via sequencing of the complete mitochondrial genome (mtGenome) and comparison of the mtGenome and mitochondrial COI barcode region to GenBank, and (b) morphology, examination of the posterior spiracles using microscopy, and comparison to published larval descriptions of blow flies. …”
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Stubble Management Plays a Greater Role than Tillage Practice in Shaping Soil Microbiome Structure and Function by Chang Xu, Ruey Toh, Jishun Li, Yi Zhou, Matthew D. Denton

“…Soil samples were collected from a long-term field trial implementing no tillage (NT) and conventional tillage (CT) with stubble retention and removal. Metagenomic sequencing facilitated the assembly of a gene catalog comprising 4.36 billion non-redundant genes. …”
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GLI1-altered mesenchymal tumor involving the parietal pleura: case report and literature review by Yuanli Zhong, Baizhou Li, Gangping Wang, Yuqing Liu, Zhenwei Chen

“…Immunohistochemical staining demonstrated positivity for CD56, S-100, and SMA. Next-generation sequencing (NGS) revealed a PTCH1-GLI1 fusion. Based on the morphological and immunophenotypic characteristics, molecular studies confirmed the diagnosis of a GLI1-altered mesenchymal tumor. …”
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Faster model-based estimation of ancestry proportions by Santander, Cindy G., Refoyo Martinez, Alba, Meisner, Jonas

“…The ADMIXTURE software is a widely used model-based approach to account for population stratification, however, it struggles with convergence issues and does not scale to modern human datasets or the large number of variants in whole-genome sequencing data. Likelihood-free approaches optimize a least square objective and have gained popularity in recent years due to their scalability. …”
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Structural and functional divergence of homoeologous genes in allopolyploid plant genomes by A. Y. Glagoleva, O. Y. Shoeva, E. K. Khlestkina

“…Thanks to the whole genome sequencing of a wide range of angiosperm species and comparative analysis of genome structure, the sequence of events that formed the genomes of modern plant taxa was restored. …”
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Transcriptomics profiling of Parkinson’s disease progression subtypes reveals distinctive patterns of gene expression by Carlo Fabrizio, Andrea Termine, Carlo Caltagirone

“…Methods Whole blood RNA-Sequencing data underwent differential gene expression analysis, followed by multiple pathway analyses. …”
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Gut Microbiota Variations between Henoch-Schonlein Purpura and Henoch-Schonlein Purpura Nephritis by Fang Zhou, Qimin Shao, Lihong Jia, Chunyan Cai

“…Fecal samples were collected and analyzed by sequencing the V3-V4 region of the 16S rRNA gene. The diversity of the fecal gut microbiota was compared between the patient groups. …”
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Colonic Adenocarcinoma with Plasmacytoid Feature: Histopathology and Molecular Characteristics of a Rare Neoplasm with an Unusual Presentation by Noor Marji, Jasrerman Dhillon, Gregory Y. Lauwers, Sebastian Feuerlein, Reza Nikfar, Monica Chatwal, Aram Vosoughi

“…Further whole-exome sequencing and whole transcriptome analysis confirmed the colorectal origin of the tumor. …”
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Post-mortem and histological lesions in the European minks (Mustela lutreola) induced by spontaneous infection with coronavirus SARS-CoV-2 by I. A. Subbotina, I. N. Gromov

“…Profound and detailed studies of the virus circulation in the European mink population and the clinical manifestations of the disease, comprehensive approaches to the disease diagnosis, including epidemiological studies, clinical and post-mortem examinations, molecular genetic laboratory diagnostics (polymerase chain reaction and full-genome sequencing) contributed to better understanding of the disease features. …”
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The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values by Qiyan Yan, Yuanyuan Chen, E-Bin Gao, Yanbo Lu, Junhua Wu, Haiyan Qiu

“…Microbial DNA was isolated from the samples followed by high throughput Illumina sequencing targeting 16 S rRNA gene. Compared to the healthy group, the RV-infected group exhibited reduced microbial diversity. …”
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LAMA2 Congenital Muscle Dystrophy: A Novel Pathogenic Mutation in Bulgarian Patient by Ivanka Dimova, Ivo Kremensky

“…Here we present a patient diagnosed postmortem as having early-onset LAMA2-related muscular dystrophy as a result of mutations in LAMA2, identified by Sanger sequencing in his parents: a novel nonsense mutation c.4452T>A in exon 31, identified in the mother, and a known pathogenic nonsense mutation c.2901C>A in exon 21, detected in the father. …”
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Ammonium oxidation by bacteria and archaea have functional implications for nitrification across a forested landscape by Jennifer Wen, Rima Upchurch, Donald R. Zak

“…Using high‐throughput sequencing, we identified the amoA of AOA and AOB from northern forest stands that occur across a natural gradient of nitrification, soil pH, and net N mineralization (i.e., NH4+ availability). …”
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Zim4rv: an R package to modeling zero-inflated count phenotype on regional-based rare variants by Xiaomin Liu, Yi-Ju Li, Qiao Fan

“…Abstract Background With the advance of next-generation sequencing, various gene-based rare variant association tests have been developed, particularly for binary and continuous phenotypes. …”
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The tetracycline resistome is shaped by selection for specific resistance mechanisms by each antibiotic generation by Kevin S. Blake, Yao-Peng Xue, Vincent J. Gillespie, Skye R. S. Fishbein, Niraj H. Tolia, Timothy A. Wencewicz, Gautam Dantas

“…We employ a high-throughput barcode sequencing protocol that can discriminate between strains in mixed culture and quantify their relative abundances. …”
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