Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity by Wen-lin Pang, Wen-lin Pang, Tian-gang Li, Tian-gang Li, Yin-ying Wang, Yin-ying Wang, Li-yun Song, Li-yun Song, Li Li, Li Li, Xiao-ya Li, Xiao-ya Li, Yong Qiu, Yong Qiu, Zhong-shan Yang, Zhong-shan Yang

“…Furthermore, 16s rRNA gene sequencing was conducted to elucidate the contribution of gut microbiota to UC pathogenesis.ResultsThe primary components of SC include Proline, Phenylalanine, Isoleucine, Lucidenic acid M, and Pyroglutamic acid. …”
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An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers by Ruyi Liu, Ruyi Liu, Ruyi Liu, Ruyi Liu, Ruyi Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Weicheng Huang, Weicheng Huang, Weicheng Huang, Weicheng Huang, Weicheng Huang, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng

“…We also performed a single-cell sequencing analysis of candidate genes via TISCH2. A correlation analysis between the Anoscore, the tumor microenvironment and drug sensitivity was conducted in GI cancers. …”
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Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Inhibit Apoptosis of Pulmonary Microvascular Endothelial Cells in COPD Mice Through miR-30b/Wnt5a Pathway by Song Q, Zhou A, Cheng W, Zhao Y, Liu C, Zeng Y, Lin L, Zhou Z, Peng Y, Chen P

“…Finally, we determined the expression of miR-30b and Wnt5a in tissues from patients with COPD.Results: BMSCs-derived exosomes could significantly reduce apoptosis of CSE-induced PMVECs and increase the expression of miR-30b (p< 0.05). Based on miR sequencing, miR-30b was highly enriched in BMSCs-derived exosomes. …”
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Multiomics profiling reveals the benefits of gamma-delta (γδ) T lymphocytes for improving the tumor microenvironment, immunotherapy efficacy and prognosis in cervical cancer by Xiaofeng Wang, Chen Li, Jie Chen, Zhiqiang Wu, Zhen Zhang, Junyi Li, Lu Yu, Yong Guan, Yuhan Zhang, Yuanjie Cao, Yancheng Liu, Hui Bai, Shaochuan Liu, Miaomiao Gao, Chenglu Lu, Zhen Tao, Zhiyong Yuan

“…As the cervix is a mucosa-rich tissue, the role of γδ T cells in cervical cancer warrants further investigation.Methods We employed a multiomics strategy that integrated abundant data from single-cell and bulk transcriptome sequencing, whole exome sequencing, genotyping array, immunohistochemistry, and MRI.Results Heterogeneity was observed in the level of γδ T-cell infiltration in cervical cancer tissues, mainly associated with the tumor somatic mutational landscape. …”
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Microdissection and Single-Cell Suspension of Neocortical Layers From Ferret Brain for Single-Cell Assays by Lucia Del-Valle-Anton, Salma Amin, Víctor Borrell

“…The genetic study of this complex structure has been commonly approached by bulk-sequencing of the entire cortex as a whole. Here, we describe the methodology to study this layered tissue in all its complexity by microdissecting two germinal layers at two developmental time points. …”
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Novel Polymorphism in a Promoter of MBL2 Gene Result in Lower MBL Expression in Chronic Infection Caused by HCV by Paywast Jamal Jalal

“…The level of these proteins was measured by ELISA method and compared with each other based on the detected SNPs by PCR and sequencing methods in the responsible genes. Our results showed that the polymorphism at position -221 in the MBL2 promoter significantly reduce the level of MBL protein more than the SNP at position -551. …”
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Diaporthaceae associated with root and crown rot of maize by S.C. Lamprecht, P.W. Crous, J.Z. Groenewald, Y.T. Tewoldemedhin, W.F.O. Marasas

“…Partial gene sequences of the translation elongation factor 1-alpha gene were added to confirm the species monophyly. …”
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Enterokinase deficiency associated with novel TMPRSS15 gene mutations: a case report by Yunxi Li, Ruijuan Li, Yanyan Pan, Weiran Zhou, Xingcui Wang, Linlin Dong, Xuemei Liu, Hongxia Zhang

“…Further examination revealed anemia, hypoalbuminemia, and multiorgan damage. Whole-exome sequencing further revealed two novel heterozygous variants of TMPRSS15: c.2611C&gt;T (p.Arg871Ter) and c.1584_1585insCTTT (p.Glu529LeufsTer2). …”
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Skin microbiome differences in pancreatic adenocarcinoma, other cancers, and healthy controls: a pilot study by Taylor Davis, Katherine T. Decker, Dana Hosseini, Gayle Jameson, Erkut Borazanci

“…While recent studies have explored the microbiome’s role in various types of cancer compared to healthy patients, this pilot study is the first to investigate differences in the skin microbiome composition among pancreatic adenocarcinoma patients, individuals with other cancers, and cancer-free controls.MethodsThe study characterizes the skin microbiome’s potential associations with cancer status by analyzing skin swabs from the forehead and cheek of 58 participants using Next Generation Sequencing (NGS), differential abundance analysis, and machine learning techniques.ResultsThe study results indicated that the cancer group displayed a significantly higher mean alpha diversity compared to the control group. …”
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Single-cell chromatin accessibility landscape profiling reveals the diversity of epigenetic regulation in the rat nervous system by Peiyao Ma, Shanshan Duan, Wen Ma, Qiuting Deng, Yeya Yu, Peng Gao, Yue Yuan, Chuanyu Liu

“…Abstract The mammalian nervous system controls complex functions through highly specialized and interacting structures. Single-cell sequencing can provide information on cell-type-specific chromatin structure and regulatory elements, revealing differences in chromatin organization between different cell types and their potential roles of these differences in brain function. …”
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Multiple programmed cell death patterns predict the prognosis and drug sensitivity in gastric cancer by Qiying Song, Shihe Liu, Di Wu, Aizhen Cai

“…The diverse patterns of programmed cell death (PCD) are significantly associated with the pathogenesis and progression of GC, and it has the potential to serve as prognostic and drug sensitivity indicators for GC.MethodThe sequencing data and clinical characteristics of GC patients were downloaded from The Cancer Genome Atlas and GEO databases. …”
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The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study by Ercan Arıcan, Hatice Kübra Kızıl Pat, Neşe Akçay, Sultan Fidan Pedük, Fahri Pat

“…Although there is a lake used by such a large population in our country, there are not enough studies on microbial diversity, so revealing the diversity is important for the lake and the population around the lake. Next-generation sequencing (NGS) research has made it possible to sequence RNA and DNA considerably quicker and cheaply than in by assessing the PCR amplicon, we utilized NGS high-throughput methods for the metagenomic research of Lake Beyşehir sequences of 16S rDNA (regions V3–V4). …”
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Effect of Relocation, Social Housing Changes, and Diarrhea Status on Microbiome Composition of Juvenile Cynomolgus Macaques (<i>Macaca fascicularis</i>) by Keely McGrew, Nicole Monts de Oca, Therese A. Kosten

“…Samples were assessed via 16S rRNA next-generation sequencing for a microbiome analysis and by ELISA for cortisol levels. …”
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Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse by Minjung Kim, Youngseok Yu, Ji-Hoi Moon, InSong Koh, Jae-Hyung Lee

“…Here, we analyzed the RNA-sequencing (RNA-Seq) datasets obtained for 18 days in two-day intervals from neonatal mouse calvarial pre-osteoblast-like cells. …”
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Tepotinib for the Treatment of Lung Adenocarcinoma Harboring MET Y1003N Point Mutation: A Case Report by Yukihiro Umeda, Satomi Kimura, Junya Kimura, Yoshiaki Imamura, Tamotsu Ishizuka

“…After a detailed examination, she was diagnosed with stage IVB lung adenocarcinoma. Next‐generation sequencing revealed an MET Y1003N mutation in the tumor. …”
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The role of candidate genetic polymorphisms in covid-19 susceptibility and outcomes by Anthony Yazbeck, Reem Akika, Zainab Awada, Nathalie K. Zgheib

“…DNA isolated from peripheral blood was genotyped for ACE1 rs1799752, ACE2 rs2074192, TMPRSS2 rs75603675 and OAS1 rs107746771 using TaqMan assays, and for TMPRSS2 rs35074065 using Sanger Sequencing. Candidate genetic variants were analyzed in association with COVID-19 susceptibility, severity, hospitalization and hypoxia, using univariate and multivariate models. …”
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A Canadian Isolate of Hepatitis D (delta) Virus by Véronique Poisson, Daniel B Ménard, Éric Frost, Jean-Pierre Perreault

“…Sequencing of four independent clones revealed 17 substitutions, corresponding to an overall sequence variability of 1%. …”
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Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia by Wisiva Tofriska Paramaiswari, Nurma Sumar Sidik, Miftahuddin Majid Khoeri, Wisnu Tafroji, Wahyu Finasari Said, Dodi Safari

“…Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). recA sequencing was used to confirm identified isolates at the genus level by MALDI-TOF MS. …”
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panniculitis in a cat from northern California by Mary Ann Lee, Dennis J Woerde, Krystle L Reagan, Tatiana G Wolf, Jane E Sykes

“…Culture of a biopsy followed by rpoB gene sequencing at a mycobacterial reference laboratory yielded Mycobacterium porcinum after 6 weeks. …”
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Emergent spinal pathologies in late-term pregnancy by Souvik Roy, Rida Mitha, Raj Swaroop Lavadi, Rohit Prem Kumar, Vinay Jaikumar, Matthew Pease, Nima Alan, Alp Ozpinar, Eren Kuris, Alan H. Daniels, Thomas J. Buell, Robert F. Heary, Nitin Agarwal, D. Kojo Hamilton

“…Several factors such as anesthesia type, positioning, and operation sequencing must be considered when managing acute spinal pathology in late-term pregnant patients, necessitating multi-disciplinary input. …”
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