PLIN1 suppresses glioma progression through regulating lipid metabolism by Kui Luo, Kai Zhuang, Hao Wu, Yuanbing Chen, Yi Liu, Fan Yang, Zhifei Wang

“…Here, we conducted RNA next-generation sequencing with 33 glioma samples and 15 normal brain samples. …”
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Synergistic effects of Rhizobium, Bacillus and Arbuscular Mycorrhizal fungi on enhancing cotton growth by Tasleem Sultana, Pavan Kumar Pindi

“…Microbial identification was performed using 16S rRNA gene sequencing. Biochemical evaluations of consortium-inoculated plants included measurements of protein, sugar, proline, and chlorophyll levels, along with IAA quantification. …”
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Fitness landscapes of human microsatellites. by Ryan J Haasl, Bret A Payseur

“…Advances in DNA sequencing technology and computation now enable genome-wide scans for natural selection to be conducted on unprecedented scales. …”
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First report of the chloroplast and mitochondrial genomes of the Indian pitcher plant, Nepenthes khasiana Hook.f. by Ruchishree Konhar, Debasis Dash, Devendra Kumar Biswal

“…These organelle genomes were assembled as part of a whole-genome sequencing project aimed at gaining deeper insights into their evolutionary relations with genomes of other carnivorous plants. …”
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p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas by Paola Campomenosi, Paola Assereto, Massimo Bogliolo, Gilberto Fronza, Angelo Abbondandolo, Antonella Capasso, Patrizia F. Bellomo, Roberto Monaco, Anna Rapallo, Andrea Sciutto, Roberto Orecchia, Elio Geido, Walter Giaretti

“…Analysis of p53 mutations spectrum of the sorted nuclei was done by Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing. Overall, we studied 20 adenocarcinomas, the corresponding control mucosa, and 7 lymph node metastases.…”
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Congenital Oral Squamous Cell Carcinoma in a Suckling Piglet by Jasmine Hattab, Abigail Rose Trachtman, Pietro Giorgio Tiscar, Marco Di Domenico, Jessica Maria Abbate, Antonio Ieni, Giuseppe Marruchella

“…However, next-generation sequencing study did not demonstrate any papillomavirus sequences in the case reported herein.…”
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A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools by Neeta Longjam, Rajib Deb, A. K. Sarmah, Tilling Tayo, V. B. Awachat, V. K. Saxena

“…Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DIVA), and real time-PCR can be used for detection of antibody against nonstructural proteins. Nucleotide sequencing for serotyping, microarray as well as recombinant antigen-based detection, biosensor, phage display, and nucleic-acid-based diagnostic are on the way for rapid and specific detection of FMDV. …”
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Effects of subclinical hypothyroidism during pregnancy on mtDNA methylation in the brain of rat offspring by Liangzhuo Xie, Yangling Huang, Xiande Ma, Xiaoqiu Ma, Jian Wang, Tianshu Gao, Wei Chen

“…Materials and methods Sixteen SD rats were randomly divided into two groups: control group (CON) and SCH group. BS-seq sequencing was used to analyze mtDNA methylation levels in the offspring’s brain tissues; the 2,7-dichlorofluorescin diacetate (DCFH-DA) probe method was employed to detect reactive oxygen species (ROS) levels in brain tissues; electron microscopy was utilized to observe the mitochondrial structure in the hippocampal tissues of the offspring. …”
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Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle by Mohammed A. Hamad, Ahmed M. Al-Shammari, Shoni M. Odisho, Nahi Y. Yaseen

“…BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. …”
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Molecular investigation of Hepatozoon canis in dogs in Mosul city by Zeena D. Zaki, Wasan A. Alobaidii

“…The total positive sample was 33 dogs out of 120 dogs (27.5%) the relationship between the infection with the parasite and the age of animals recorded a high prevalence of infection in dogs less than 6 months while the lowest prevalence of infection in animals more than 2 years old, The male dogs showed a high prevalence of infection when compared with female dog, The large breeds dogs recorded high prevalence of infection than small breeds dogs, The outdoor dogs showed a high prevalence of infection Animals which suffer from paleness in mucous membrane showed high prevalence, while healthy animals recorded lowest prevalence of infection, gene sequence showed there were only three isolates out of the total samples sent for genetic sequencing.…”
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Placental Findings in Lysosomal Storage Disease Diagnosis: A Case Report of Galactosialidosis by Sasha Libbrecht, Francois Eyskens, Sabine Declercq, Cecile Colpaert

“…The diagnosis was confirmed by massive parallel sequencing, revealing a single nucleotide variation in the CTSA gene (c.265A>C, p.Ser89Arg). …”
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Nidoviruses associated with aquatic animals by L. P. Buchatsky, V. V. Makarov

“…Representatives of the other families of aquatic animal nidoviruses were identified using metagenomic deep sequencing (metagenomics), but their effect on the host organism has not yet been adequately studied. …”
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Altered microbiome and metabolome profiling in fearful companion dogs: An exploratory study. by Luigi Sacchettino, Michele Costanzo, Iolanda Veneruso, Valeria D'Argenio, Maria Mayer, Francesco Napolitano, Danila d'Angelo

“…Here, taking advantage of the next generation sequencing approach, we sought to investigate the potential connection between gut microbiota and microbiome in dogs suffering from generalized fear (n = 8), when compared to healthy subjects (n = 8), who all lived in different families. …”
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Intratumoural CD8+ CXCR5+ follicular cytotoxic T cells have prognostic value and are associated with CD19+ CD38+ B cells and tertiary lymphoid structures in colorectal cancer by Fangze Wei, Xiaotian Xu, Jing Wang, Shi Wen Mei, Fu Qiang Zhao, Fei Huang, Ti Xian Xiao, Guo Jing Wang, Baojun Wei, Shengkai Huang, Wei Cui

“…By combining single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing, we explored the potential function of TFC cells and developed a prediction model for CRC. …”
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The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers by Xian-Yan Yang, Nian Chen, Qian Wen, Yu Zhou, Tao Zhang, Ji Zhou, Cheng-Hui Liang, Li-Ping Han, Xiao-Ya Wang, Qing-Mei Kang, Xiao-Xia Zheng, Xue-Jia Zhai, Hong-Ying Jiang, Tian-Hua Shen, Jin-Wei Xiao, Yu-Xin Zou, Yun Deng, Shuang Lin, Jiang-Jie Duan, Jun Wang, Shi-Cang Yu

“…Single-cell RNA sequencing (scRNA-seq), spatially resolved transcriptomics (SRT), and multiplex immunofluorescence (mIF) staining analyses verified MCI. …”
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Interpreting Variants of Uncertain Significance in PCD: Abnormal Splicing Caused by a Missense Variant of DNAAF3 by Haixia Zheng, Chongsheng Cheng, Miao He, Wangji Zhou, Yixuan Li, Jinrong Dai, Ting Zhang, Kai‐Feng Xu, Xue Zhang, Xinlun Tian, Yaping Liu

“…Methods Whole exome sequencing (WES) and Sanger sequencing were conducted to identify potential pathogenic variants of PCD. …”
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Germline structural variant as the cause of Lynch Syndrome in a family from Ecuador by Gemma Llargués-Sistac, Laia Bonjoch, Jenifer Muñoz, Xavier Domínguez-Rovira, Teresa Ocaña, Maria Isabel Alvarez-Mora, Celia Badenas, Anna Esteve-Codina, Carlos Reyes-Silva, Gabriela Jaramillo-Koupermann, Maria Teresa Rodrigo, Sandra López-Prades, Miriam Cuatrecasas, Antoni Castells, Francesc Balaguer, Leticia Moreira, Guerau Fernandez, Sergi Castellví-Bel

“…Whole-genome sequencing and transcriptomics allowed the identification of the genomic rearrangement and highlights the importance of the use of these additional approaches to achieve a comprehensive molecular diagnosis in some LS patients.…”
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Genetic and Molecular Characterization of Avian Influenza A(H9N2) Viruses from Live Bird Markets (LBM) in Senegal by Mamadou Malado Jallow, Moussa Moise Diagne, Marie Henriette Dior Ndione, Mamadou Aliou Barry, Ndiendé Koba Ndiaye, Davy Evrard Kiori, Marie Pedapa Mendy, Déborah Goudiaby, Gamou Fall, Malick Fall, Ndongo Dia

“…Subsequently, a subset of H9-positive samples was selected for whole sequencing. From December 2023 to October 2024, 499 samples were tested, and AIV was detected in 58.3% of them. …”
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An exploratory study of INPP4B, a biomarker of gemcitabine chemoresistance in pancreatic cancer by REN Jiaqiang, WU Shuai, SU Tong, LI Jie, HAN Liang, WU Zheng

“…Second-generation high-throughput RNA-seq sequencing was conducted on these resistant cells, and bioinformatics analysis was employed to identify four pancreatic cancer genes from the Gene Expression Omnibus (GEO) datasets (GSE106336, GSE110580, GSE35141, and GSE140077). …”
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Leveraging genomic insights from the neglected malaria parasites P. malariae and P. ovale using selective whole genome amplification (SWGA) approach by Fathia Ben-Rached, Amit Kumar Subudhi, Chang Li, Mariah Alawi, Rohit Satyam, Sui Xu, Guoding Zhu, Raeece Naeem, Sara Mfarrej, Di Liu, Zenaida Stead, Caroline Askonas, Yaobao Liu, Jun Cao, Arnab Pain

“…Techniques that cost-effectively allow the enriching of the genome of interest from complex genome backgrounds for sequencing studies help immensely to perform genomic analyses. …”
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