Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1 by Shengkai Jin, Yuhua Zhou, Jing Lv, Yichen Lu, Yuwei Zhang, Menglu Li, Ninghan Feng

“…Subsequently, we investigated the mechanism of action of IMP using transcriptome sequencing, qPCR, and Western blot analysis. Finally, we validated our findings in vivo using a mouse model. …”
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Molecular anti-tumorigenic mechanism of Radix Polygoni Multiflori-derived exosome-like nanoparticles by Mingqing Yang, Lining Xu, Weiyu Wang

“…This indicated that RPM-ELNs could be internalized by hepatoma cells effectively and inhibit their proliferation and migration in vitro. RNA sequencing and qPCR experiments were performed, and the results of bioinformatic analyses revealed that RPM-ELNs could globally regulate expression levels of numerous genes associated with cancer and the cell cycle. …”
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Inflammatory biomarkers and therapeutic potential of milk exosome-mediated CCL7 siRNA in murine intestinal ischemia-reperfusion injury by WenDong Chen, WenPing Xu, Li Ma, Chun Bi, MeiXu Yang, Wei Yang

“…Despite advances in understanding the pathophysiology of IIRI, effective diagnostic and therapeutic strategies remain limited.MethodsUsing transcriptome sequencing in a mouse model of IIRI, we identified potential biomarkers that were significantly upregulated in the IIRI group compared to the sham group. …”
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Computational thinking for young indigenous learners in New Zealand by Wendy Fox‐Turnbull, Shaoqun Wu, Tiana Mayo, Matthew Stafford, Swati Gulati

“…Technology practice is most successful when embedded within authentic contexts, thus this paper presents a study that facilitated the learning of two concepts of computational thinking: sequencing and orientation within culturally embedded technology practice. …”
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CCI: A Consensus Clustering-Based Imputation Method for Addressing Dropout Events in scRNA-Seq Data by Wanlin Juan, Kwang Woo Ahn, Yi-Guang Chen, Chien-Wei Lin

“…Single-cell RNA sequencing (scRNA-seq) is a cutting-edge technique in molecular biology and genomics, revealing the cellular heterogeneity. …”
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Single-Cell Transcriptome Analysis Profile of Meniscal Tissue Macrophages in Human Osteoarthritis by Jingbin Zhou, Zhihong Zhao, Chen He, Feng Gao, Yu Guo, Feng Qu, Yujie Liu

“…Here, we have used single-cell RNA sequencing analysis to gain insight into the heterogeneity and functional specialization of human knee macrophages. …”
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The relationship between mutation carriage of BRCA1/2 and clinicopathological characteristics in women with breast cancer – experience from a diagnostic centre in Turkey by Neslihan Duzkale, Onur Can Guler, Suat Kutun, Canan Emiroglu, Serdar Saridemir, Aysun Gokce, Olcay Kandemir, Tugba Taskın Turkmenoglu, Serap Yorubulut, Bahadır Kulah

“…In 170 female patients with BC, BRCA1/2 genes were investigated using Next Generation Sequencing. Demographic and clinicopathological characteristics of the patients and correlations of pedigree analysis with BRCA1/2 mutation status were analysed. …”
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Imatinib-resistance without BCR/ABL Point Mutation in Chronic Myeloid Leukemia by Aytan Shirinova

“…The patient did not achieve a major molecular response (MMR). Next-generation sequencing using a 54 myeloid-targeted gene panel was negative for ABL1 L248V, G250E, Y253H, E255K, F311L, T315I, F317L, F311I, M351T, and other point mutations. …”
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Genomic analysis of antimicrobial resistant Escherichia coli isolated from manure and manured agricultural grasslands by C. Tyrrell, C. M. Burgess, F. P. Brennan, D. Münzenmaier, D. Drissner, R. J. Leigh, F. Walsh

“…The E. coli isolates were phenotypically antimicrobial resistant, predominantly multidrug resistant. Whole genome sequencing identified 31 antimicrobial resistance genes, and mutations in the gyrA, parC, and parE genes, conferring fluoroquinolone resistance. …”
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Covariation Between Microbiome Composition and Host Transcriptome in the Gut of Wild Drosophila melanogaster: A Re‐Analysis by Frances Llanwarne, Adam J. Dobson

“…Previous work in wild male D. melanogaster guts analysed paired measurements of (A) bacterial diversity and abundance, measured by 16S amplicon sequencing; and (B) the host gut transcriptome, but no signature of covariation was detected. …”
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Cryptic coral community composition across environmental gradients. by Gia N Cabacungan, Tharani N Waduwara Kankanamalage, Amilah F Azam, Madeleine R Collins, Abigail R Arratia, Alexandra N Gutting, Mikhail V Matz, Kristina L Black

“…Croix, USVI based on 2b-RAD sequencing data. We then analyzed associations between the distributions of cryptic lineages across the six coral species (i.e., "cryptic coral community composition") and ecoregions, or geographically distinct environmental conditions. …”
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Comparison of the Genetic Alterations between Primary Colorectal Cancers and Their Corresponding Patient-Derived Xenograft Tissues by Sang Mi Yu, Seung-Hyun Jung, Yeun-Jun Chung

“…In this study, we analyzed the genetic alterations of three primary colorectal cancers (CRCs) and matched xenograft tissues in PDX models using a next-generation sequencing cancer panel. Of the 17 somatic mutations identified from the three CRCs, 14 (82.4%) were consistently identified in both primary and xenograft tumors. …”
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Coexistence of T-Cell Lymphoblastic Lymphoma and Ichthyosis Vulgaris: A Case Report by Şule Çalışkan Kamış, Begül Yağcı

“…A homozygous variant in the DSG1 gene was detected through whole-exome sequencing. The diagnosis of HIES was confirmed through clinical evaluation, including elevated serum IgE levels and associated symptoms. …”
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Isolation of Lodderomyces elongisporus from the Catheter Tip of a Fungemia Patient in the Middle East by Suhail Ahmad, Zia U. Khan, Molly Johny, Najat M. Ashour, Wehad H. Al-Tourah, Leena Joseph, Rachel Chandy

“…The identity of the isolate was confirmed by phenotypic characteristics and ribosomal DNA sequencing.…”
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Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid by Marjolein J. G. Hooykaas, Paul J. J. Hooykaas

“…Unexpectedly, we found evidence that a natural isolate from a rose crown gall, called NBC51/LBA8980, was a bacterium that did not belong to the <i>Rhizobiaceae</i> family. Whole-genome sequencing revealed that this bacterium contained three large DNA circles with rRNA and tRNA genes, representing one chromosome and two chromids, respectively, and two megaplasmids, including a Ti plasmid. …”
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Taxonomic and Functional Dynamics of Bacterial Communities During Drift Seaweed Vermicomposting by Manuel Aira, Ana Gómez-Roel, Jorge Domínguez

“…This study aimed to investigate the dynamics of the taxonomic and functional bacterial communities in seaweed during the vermicomposting process by high-throughput sequencing of 16S rRNA gene amplicons. Vermicomposting changed the composition of the bacterial communities, as indicated by the low proportion of bacterial taxa common to the bacterial communities in the raw seaweed and vermicompost (21 to 56 ASVs from more than 900 ASVs per sample type). …”
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Disruption of the β-catenin destruction complex via Ephexin1-Axin1 interaction promotes colorectal cancer proliferation by Jeeho Kim, Young Jin Jeon, In-Youb Chang, Jung-Hee Lee, Ho Jin You

“…Through comprehensive analysis, including RNA sequencing data from TCGA and functional assays, we observed that Ephexin1 promotes tumor proliferation and migration by activating the Wnt/β-catenin pathway. …”
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Composition and functional diversity of soil and water microbial communities in the rice-crab symbiosis system. by Guo Yang, Tian Juncang, Wang Zhi

“…In order to clarify the structure and function of soil and water microbial communities in the rice-crab symbiosis system, the standard rice-crab field with a ring groove was used as the research object. High-throughput sequencing was performed with rice field water samples to analyze the species and abundance differences of soil bacteria and fungi. …”
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Gene Mutation and Its Association with Clinicopathological Features in Young Patients with Non-Small-Cell Lung Cancer by Wencui Kong, Zongyang Yu, Wenwu Wang, Jingrong Yang, Jingfang Wang, Zhongquan Zhao

“…Clinicopathologic information of 102 young NSCLC patients was collected. Direct ctDNA sequencing of a portion of these patients was performed. …”
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Modern and future forestry based on biotechnology by Shihui Niu, Jihua Ding, Changzheng Xu, Jing Wang

“…In the last 2 decades, advances in biotechnologies such as high‐throughput sequencing and genome editing are transforming tree breeding and forestry. …”
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